Supplementary Figure 1: Genes are differentially expressed in TAMs versus normal
macrophages and gene overexpression in TAMs is compared to M1 macrophages A)
TAMs were isolated from 4T1 tumor tissue with biotin labeled F4/80 antibody by
MACS, sorted and identified by FACS analysis. After sorting, the purity of macrophages
is > 95%; (B). Macroarray data were subjected to SAM analysis to determine the
significance of gene expression and the heat map of the genes served to determine those
genes that are significant by SAM analysis and clustered into groups of TAMs and
normal macrophages. Green to red = low to high expression; (C) Identification of cluster
genes over-expressed by TAMs. *Ratio is the number of focus genes divided by the
number of genes in the canonical pathway; (D). Groups of genes overexpressed in TAMs
were compared to those in M1 macrophages by DNA array analysis. E. Identification of
cluster genes overexpressed by TAMs.
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