PCR Cloning Quotation Request Form Instructions 1. Please complete and email this form together with the supporting materials to gene@genscript.com 2. Our service representative will contact you with the quote Customer Information Name: Institution: Shipping address (needed to determine shipping cost): Phone: Email: Template Information Template Type: Plasmid cDNA library Genomic DNA Template Name: Species: Length: Template Sequence: Do you want GenScript to sequence your gene in the starting construct ($25/run, ~700 bases/run)? Yes No Other Information: Template Vector Information* Vector Name: Manufacturer: Size: Copy Number: High Low Antibiotic Marker: Restriction sites used to clone your gene: Gene Orientation: Other Information: *Please include template construct map with this form. If the vector is not commercially available, please include complete construct sequence. Destination Vector Information * Vector Name: Manufacturer: Size: Copy Number: High Low Antibiotic Marker: Other Information: *If you are supplying your own, non-commercial vector, please include complete vector sequence and map with this form. Cloning Strategy Restriction sites to be used for cloning: Desired insert sequence: Desired sequence orientation: Other Information: Quotation Request Checklist* Please make sure that the following materials and data are included with your quotation request: 1. Template sequence 2. Map of the template construct, including both your gene and vector (if available). If the vector is not commercially available, please include complete construct sequence. 3. Map and complete sequence of the destination vector (if supplying your own, non-commercial vector) 4. Desired insert sequence *The following starting materials will be required when you submit your order to GenScript: 1. Template (~ 5ug of plasmid DNA, DNA library, or genomic DNA; concentration 50-100 ng/ul) 2. Destination vector, 5 ug of plasmid DNA (if supplying your own vector) Project Information Is this project for grant application purpose? When will the project start? Immediately Yes Within one month No Within three months Half a year later