OHS017
OHS Risk Assessment and
Control Form
Risk assessment completed by: Jaa Y. New
Staff/student number: z3301761
Faculty/Division:
School/Unit:
Medicine
STGCL
Document number
Initial Issue date
30/03/2010
STGCL.RA.48.1
Current version
1.0
Current Version
30/03/2010
Next review date
March 2012
For additional information refer to the OHS Risk Assessment and Control Procedure, the OHS Risk Rating Procedure and the Hierarchy of Risk Controls.
Risk Assessment title: Protein purification from bacteria cells.
Step 1: Identify the activity
Describe the activity:
This Risk Assessment describes protein purification from bacteria cells.
Describe the location:
Medicine Lab, Rm 3.20, Level 3., Research & Education Centre, 4-10 South St., Kogarah. NSW 2217.
Step 2: Identify who may be at risk by the activity
The person carrying out these procedures are at risk.
Steps 3 to 7: Identify the hazards, risks, and rate the risks
1. An activity may be divided into tasks. For each task identify the hazards and associated risks.
2. List existing risk controls and determine a risk rating using the UNSW Risk Rating Procedure.
3. Additional risk controls may be required to achieve an acceptable level of risk. Re-rate the risk if additional risk controls used.
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Page 1 of 5
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6 16/07/2008
Tasks
Hazards
Associated risks
(Step 3)
(Step 4)
Risk rating with existing
controls *
Additional risk controls
required
Risk Rating with additional
controls *
(Step 5)
(Step 6)
(Step 7)
Existing risk controls
C
L
R
Handling of bacteria
culture.
Biological exposure
Handling of bacteria
outside of a Class I
Biohazard Safety Cabinet
risks generating aerosol
particles which may be
inhaled or ingested
An SWP has been
prepared. External and
internal PC-2 training is
compulsory. Conduct
bacteria work in a Class I
Biohazard Safety Cabinet
Wear suitable protective
clothing, goggles and
gloves.
3
E
M
Handling Dithiothreitol
(DTT)
Hazardous substances,
Irritant to eyes.
Toxic by inhalation, in
contact with skin and if
swallowed.
MSDSs are available.
Wear suitable protective
clothing, goggles and
gloves.
2
D
L
Handling of Ammonium
Hydroxide
Hazardous substances,
Causes burns. Harmful
by inhalation or
swallowed or absorbed
by skin.
MSDSs are available.
Wear suitable protective
clothing, goggles and
gloves.
2
D
L
Handling of NP-40
Hazardous substances
Harmful if swallowed.
Risk of serious damage
to eyes.
MSDSs are available.
Wear suitable protective
clothing, goggles and
gloves to prevent contact
with eyes and skin.
2
D
L
Handling of Isopropyl-ßD-thiogalactoside (IPTG)
Hazardous substances
Highly flammable.
Repeated exposure may
cause skin dryness or
cracking.
MSDSs are available.
Wear suitable protective
clothing, goggles and
gloves.
2
D
L
Protease Inhibitor PMSF
Hazardous substances
Harmful. Irritating to
respiratory system and
skin.
MSDSs are available.
Wear suitable protective
clothing, goggles and
gloves.
2
D
L
Triton X-100
Hazardous substances
Harmful if swallowed.
Risk of serious damage
to eyes.
MSDSs are available.
Wear suitable protective
clothing, goggles and
gloves.
2
D
L
(Apply the hierarchy of
risk controls)
C
L
R
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Page 2 of 5
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6 16/07/2008
* C = consequence
L = likelihood
R = risk rating
from the UNSW Risk Rating Procedure
Step 8 Documentation and supervisor approval
Completed by: Jaa Y. New
(signature)
Authorised by:
Prof Beng Chong (Medicine)
(signature)
Date:30/03/2010
Reviewed by:
(signature)
Authorised by
(signature)
Date:
Reviewed by:
(signature)
Authorised by
(signature)
Date:
Reviewed by:
(signature)
Authorised by
(signature)
Date:
Reviewed by:
(signature)
Authorised by
(signature)
Date:
Step 9: Implement the additional risk controls
identified
Indicate briefly what additional risk controls from Step 6 above were implemented, when and by whom.
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Risk control:
Date:
Implemented by:
Step 10: Monitor and review the risk controls
It is important to monitor risk controls and review risk assessments regularly. Review is required when there is a change in the process, relevant legal changes, and where a cause for concern has
arisen. Reviews could be scheduled on an annual basis. If the risk assessment has substantially changed a new risk assessment is warranted.
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
Review date:
Reviewed by:
Authorised by:
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Page 3 of 5
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6 16/07/2008
Documentation
It is a requirement that legal and advisory documentation that supports this risk assessment be listed. Such documentation includes Acts, Regulations, Australian Standards and Codes of Practice,
where applicable.
Australian/New Zealand Standard, Safety in Laboratories Part 3: Microbiological aspects and containment facilities 2002 (AS/NZS 2243.3: 2002)
MSDSs for all the listed chemicals.
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Page 4 of 5
Risk Assessment and Control Form
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6 16/07/2008
UNSW Concise OHS Risk Rating Table
OHS697
What you need to do
1. Consider what can go wrong that can hurt someone
2. Determine what the most likely outcome would be - Consequences
3. Determine how likely those consequences are - Likelihood
4. Calculate the risk rating
5. Required action
How severely could someone be hurt
death or permanent disability to one or more persons
hospital admission required
medical treatment required
first aid required
injuries not requiring first aid
CONSEQUENCES:
Severe
Major
Moderate
Minor
Insignificant
How likely are those consequences?
expected to occur in most circumstances
will probably occur in most circumstances
could occur at some time
is not likely to occur in normal circumstances
may occur only in exceptional circumstances
LIKELIHOOD:
Almost certain
Likely
Possible
Unlikely
Rare
CONSEQUENCES
Insignificant
1
Minor
2
Moderate
3
Major
4
Severe
5
M
H
H
VH
VH
M
M
H
H
VH
Possible
C
L
M
H
H
VH
Unlikely
D
L
L
M
M
H
Rare
E
L
L
M
M
M
LIKELIHOOD
Almost
certain
A
Likely
B
Risk level
Very high
High
Medium
Low
Required action
Act immediately:
The proposed task or process activity must not proceed. Steps must be taken to lower the risk level to as
low as reasonably practicable using the hierarchy of risk controls.
Act today:
The proposed activity can only proceed, provided that:
(i) the risk level has been reduced to as low as reasonably practicable using the hierarchy of
risk controls;
(ii) the risk controls must include those identified in legislation, Australian Standards, Codes of
Practice etc.
(iii) the risk assessment has been reviewed and approved by the Supervisor and
(iv) a Safe Working Procedure or Safe Work Method has been prepared.
(v) The supervisor must review and document the effectiveness of the implemented risk
controls.
Act this week:
The proposed task or process can proceed, provided that:
(i) the risk level has been reduced to as low as reasonably practicable using the hierarchy of
risk controls;
(ii) the risk assessment has been reviewed and approved by the Supervisor and
(iii) a Safe Working Procedure or Safe Work Method has been prepared.
Act this month:
Managed by local documented routine procedures which must include application of the hierarchy of
controls.
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Page 5 of 5
UNSW Concise OHS Risk Rating Table
Effective date: 01/01/2007
Uncontrolled document when printed
Current Version: 2.6,16/07/2008