The first 7 Years of Ultra-Performance Liquid Chromatography (UPLC):
Scientific Achievements and Daily Work Challenges.
Shulamit Levin
Medtechnica, Israel
shula_levin@medtechnica.co.il
In 2004, the first UPLC® chromatograph was introduced by Waters and by 2011 the rest of the LC
world managed to catch up, as each of the top seven LC vendors had introduced UHPLC type of
instrument. It seems that the promised benefits of simultaneous improvement in sensitivity,
resolution and throughput were real and often more valuable than expected. The presentation will
overview what has UPLC brought to science but at the same time will discuss some everyday work
challenges for its users.
First of all UPLC has become the first choice of LC inlet for LC-MS and LC-MS/MS as will be
demonstrated here. Second, UPLC brought about achievements in the field of pharmaceutics
(cleaning validation, in-process analysis, stability indicating methods, accelerated method
development by QbD, etc), biotechnology (proteomics, peptide mapping, amino acid analysis,
glycan and oligonucleotides) food safety (pesticides and residues), agriculture (metabolomic),
environmental and forensic sciences.
Such separations could be accomplished when the users succeeded to overcome the challenges of
method development and transfer from HPLC to UPLC: rational scaling down and meticulous
operation of the instrumentation.
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