Electronic Supplementary Material Direct electrochemistry of glucose oxidase and a biosensor for glucose based on a glass carbon electrode modified with MoS2 nanosheets decorated with gold nanoparticles Shao Su, Haofan Sun, Fei Xu, Lihui Yuwen, Chunhai Fan*, Lianhui Wang* Table S1. The comparsion of the performance of prepared sensor and others reported in the literatures for glucose detection. Glucose biosensor Linear range (mM) Detcti Ref. on limit (μM) Gox-AuNPs@MoS2 0.01-0.3 2.8 This work GOx-graphene 0.1-10 10±2 [1] GOx-graphene-AuNPs-chi 2-10 180 [2] GOx-graphene-chitosan 0.08-12 20 [3] Nafion-GOx-GNSs 0.2-1.4 - [4] GOx-MWCNTs-chitosan 0-7.8 - [5] GOx-colloidal gold 0.08-0.28 10 [6] GOx-CNTs 0-30 80 [7] Nafion-GOx-CNTs-CdTe 0-0.7 - [8] GOx-sol-gel-CNTs 0.2-20 50 [9] GOx-PAN 0-5 20 [10] Nafion-GOx-OMC 0.5-1.5 156.62 [11] tosan Abbreviation: [AuNPs] gold nanoparticles, [GNSs] graphite nanosheets, [MWCNTs] multi-walled carbon nanotubes, [CNTs] carbon nanotubes, [PAN] microporous polyacrylonitrile, [OMC] ordered mesoporous carbon. 0 -4 500M -3 -10 -5 -6 -7 -20 -8 10M -0.6 0 100 200 300 400 Concentration/M -0.4 500 -0.2 E/V (vs. SCE) Figure S1. Cyclic voltammograms of Nafion-GOx-AuNPs@MoS2/GCE in the presence of various concentrations of glucose in the O2-saturated human serum. The inset is the calibration curve corresponding to amperometric responses. Scan rate: 100 mV·s-1. References: 1. Wu P, Shao Q, Hu Y, Jin.J, Yin.Y, Zhang.H Cai. C (2010), Direct electrochemistry of glucose oxidase assembled on graphene and application of glucose detection. Electrochim Acta. 55: 8606-8614. DOI: 10.1016/j.electacta.2010.07.079. 2. Shan CS., Yang HF, Han DX, Zhang QX, Ivaska A, Niu L, (2010) Graphene/AuNPs/chitosan nanocomposite film for glucose biosensing. Biosens. Bioelectron. 25: 1070-1074. DOI: 10.1016/j.bios.2009.09.024. 3. Kang XH, Wang J, Wu H, Aksay IA, Liu J, Lin YH, (2009) Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. Biosens. Bioelectron. 25: 901-905. DOI: 10.1016/j.bios.2009.09.004. 4. Fu CL, Yang WS, Chen X, Evans DG, (2009) Direct electrochemistry of glucose oxidase on a graphite nanosheet-Nafion composite film modified electrode, Electrochem. Commun. 11: 997-1000. DOI: 10.1016/j.elecom.2009.02.042. 5. Liu Y, Wang MK, Zhao F, Xu ZA, Dong SJ, (2005) The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix. Biosens. Bioelectron. 21:984-988. DOI: 10.1016/j.bios.2005.03.003. 6. Liu SQ, Ju HX, (2003) Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. Biosens. Bioelectron. 19: 177-183. DOI: 10.1016/S0956-5663(03)00172-6. 7. Lin YH, Lu F, Tu Y, Ren ZF, (2004) Glucose biosensors based on carbon nanotube nanoelectrode ensembles. Nano Lett. 4: 191-195. DOI: 10.1021/nl0347233. 8. Liu Q, Lu X, Li J, Yao X, Li JH, (2007) Direct electrochemistry of glucose oxidase and electrochemical biosensing of glucose on quantum dots/carbon nanotubes electrodes. Biosens. Bioelectron. 22: 3203-3209. DOI: 10.1016/j.bios.2007.02.013. 9. Salimi A, Compton RG, Hallaj R, (2004) Glucose biosensor prepared by glucose oxidase encapsulated sol-gel and carbon-nanotube-modified basal plane pyrolytic graphite electrode. Anal. Biochem. 333: 49-56. DOI: 10.1016/j.ab.2004.06.039. 10. Zheng H, Xue H, Zhang Y, Shen Z, (2002) A glucose biosensor based on microporous polyacrylonitrile synthesized by single rare-earth catalyst. Biosens. Bioelectron. 17: 541-545. DOI: 10.1016/S0956-5663(02)00010-6. 11. Zhou M, Shang L, Li BL. Huang LJ, Dong SJ, (2008) Highly orderly mesoporous carbons as electrode material for the construction of electrochemical dehydrogenaseand oxidase-based biosensors. Biosens. Bioelectron. 24: 442-447. DOI: 10.1016/j.bios.2008.04.025.