导师介绍
基本资料
陈健
博士、副研究员、硕士生导师，浙江理工大学骨干教师。
1995.09~1999.06
华中农业大学 畜牧兽医学院兽医专业
本科
1999.09~2006.03
浙江大学 生物化学与分子生物学专业 博士
2006.04~2008.08
浙江理工大学 生命科学学院 助理研究员
2008.09~至今
浙江理工大学 生命科学学院 副研究员
其他学术、社会兼职：
中国生物化学与分子生物学学会会员
主讲课程
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《基因工程》、《基因工程大实验》和《核酸技术》等
主要研究方向
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方向 1：杆状病毒分子生物学
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方向 2：家蚕生物反应器
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方向 3：家蚕功能基因
科研项目
1. 浙江省自然科学基金项目，复制缺陷型家蚕BmNPV高通量载体表达系统的构建及应用
研究，Y309028，10万，2010.01~2011.12，排名1
2. 浙江省自然科学基金项目，家蚕生物反应器高效表达蛇毒金属蛋白酶 Agkistin 及其抗血
栓等功能的研究 Y305171，5 万，2006.01~2007.12, 排名 1
3. 国家 863 项目，家蚕生物反应器蛋白质药物规模化制备技术平台 2007AA021703，819
万，2007.07~2010.11，排名 1
4. 国家自然科学基金，家蚕中 microRNA 的基因鉴定和功能研究 30670477，25 万，
2006.01~2009.12，排名 4
5. 国家 973 项目，家蚕小分子 mRNA、小肽功能以及病原体与宿主相互作用机理研究
2005CB121006，160 万，2005.12~2010.12，排名 2
6. 国家科技支撑计划项目，人禽流感防治新型药物和疫苗的应急研究和开发--家蚕生物反
应器生产新型人用禽流感疫苗的试制 2006BAI01B04，700 万，2007.01-2007.12，排名
5
发表论文
1. Chen J, Nie ZM, Lv ZB, Zhu CG, Xu CZ, Jin YF, Wu XF, Zhang YZ. Large-Scale
Purification of hGM-CSF Expressed in Bombyx mori Pupae. Applied Biochemistry and
Biotechnology. 2007 Apr;141(1):149-160. (SCI, IF: 1.102)
2. Chen J, Liu T, Zhu CG, Jin YF, Zhang YZ. Genetic variation of Chinese PRRSV Strains
based on ORF5 sequence. Biochemical Genetics. 2006 Oct, 44(9-10): 425-435. (SCI, IF:
0.876)
3. Chen J, Wu XF, Zhang YZ. Expression, purification and characterization of human
GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor. Journal of Biotechnology.
2006 May 17; 123(2): 236-247. (SCI, IF: 2.6)
4. Lan H, Nie Z, Liu Y, Lv Z, Liu Y, Quan Y, Chen J, Zhen Q, Chen Q, Wang D, Sheng Q, Yu
W, Chen J, Wu X, Zhang Y. In Vivo Bioassay of Recombinant Human Growth Hormone
Synthesized in B. mori Pupae. J Biomed Biotechnol. 2010;2010:306462.
5. Zeng F, Xie H, Nie Z, Chen J, Lv Z, Chen J, Wang D, Liu L, Yu W, Sheng Q, Wu X, Zhang
Y. Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the
Silkworm, Bombyx mori. Comp Funct Genomics. 2009:947490.
6. Nie Z, Lv Z, Qian J, Chen J, Li S, Sheng Q, Wang D, Shen H, Yu W, Wu X, Zhang Y.
Molecular cloning and expression characterization of translationally controlled tumor protein
in silkworm pupae. Mol Biol Rep. 2009 Sep 15.
7. Shen Y, Zhang T, Chen J, Lv Z, Chen J, Wang D, Nie Z, He P, Wang J, Zheng Q, Sheng Q,
Wu X, Zhang Y. Molecular Characterization and Tissue Localization of an F-Box Only
Protein from Silkworm, Bombyx mori. Comp Funct Genomics. 2009:416040.
8. Zhang Y, Xia Q, Xu J, Chen J, Nie Z, Wang D, Zhang W, Chen J, Zheng Q, Chen Q, Kong
L, Ren X, Wang J, Lv Z, Yu W, Jiang C, Liu L, Sheng Q, Jin Y, Wu X. Aligning the
proteome and genome of the silkworm, Bombyx mori. Funct Integr Genomics. 2009
Nov;9(4):447-54. (SCI)
9. Jin R, Lv Z, Chen Q, Quan Y, Zhang H, Li S, Chen G, Zheng Q, Jin L, Wu X, Chen J,
Zhang Y. Safety and immunogenicity of H5N1 influenza vaccine based on baculovirus
surface display system of Bombyx mori. PLoS One. 2008;3(12):e3933.
10. Nie Z, Zhou R, Chen J, Wang D, Lv Z, He P, Wang X, Shen H, Wu X, Zhang Y.
Subcellular localization and RNA interference of an RNA methyltransferase gene from
silkworm, bombyx mori. Comp Funct Genomics. 2008:571023. (SCI)
11. He PA, Nie Z, Chen J, Chen J, Lv Z, Sheng Q, Zhou S, Gao X, Kong L, Wu X, Jin Y,
Zhang Y. Identification and characteristics of microRNAs from Bombyx mori. BMC
Genomics. 2008 May 28;9:248. (SCI)
12. Zhang Y, Lv Z, Chen J, Chen Q, Quan Y, Kong L, Zhang H, Li S, Zheng Q, Chen J, Nie Z,
Wang J, Jin Y, Wu X. A novel method for isolation of membrane proteins: A baculovirus
surface display system. Proteomics. 2008 Sep 22;8(20):4178-4185. (SCI)
13. Nie Z, Xu J, Chen J, Lv Z, Wang D, Sheng Q, Wu Y, Wang X, Wu X, Zhang Y.
Expression Analysis and Characteristics of Profilin Gene from Silkworm, Bombyx mori.
Appl Biochem Biotechnol. 2008,158(1):59-71. (SCI)
14. Jianqing Chen, Jian Chen, Qijing Gai, Zhengbing Lv, Dan Wang, Zuoming Nie, Jiang
Wang, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Molecular Characterization and
Immunohistochemical Localization of a Novel Troponin C During Silkworm Development.
Cell Tissue Res. 2008 Mar;331(3):725-738. (IF: 2.58).
15. Lingyin Kong, Zhengbing Lv, Jian Chen, Zuoming Nie, Dan Wang, Hongdan Shen,
Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Expression analysis and tissue distribution of
two 14-3-3 proteins in silkworm (Bombyx mori). BBA-General subject, 2007
Dec;1770(12):1598-1604. (IF: 2.02).
16. Wang X, Chen J, Lv Z, Nie Z, Wang D, Shen H, Wang X, Wu X, Zhang Y. Expression and
Functional Analysis of CRABP from Silkworm Pupae (Bombyx mori). Journal of Cellular
Biochemistry, 2007 Nov 1; 102(4): 970-997. (SCI, IF: 3.591)
17. Nie ZM, Zhang ZF, Wang D, He PA, Jiang CY, Song L, Chen F, Xu J, Yang L, Yu LL,
Chen J, Lv ZB, Lu JJ, Wu XF, Zhang YZ. Complete sequence and organization of
Antheraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. BMC Genomics. 2007 Jul 24;
8: 248. (SCI, IF: 4.01)
18. Zhang YZ, Chen J, Nie ZM, Lu ZB, Wang D, Jiang CY, He PA, Liu LL, Lou YL, Song L,
Wu XF. Expression of open reading frames in silkworm pupal cDNA library. Applied
Biochemistry and Biotechnology, 2007 Mar;136(3):327-343. (SCI, IF: 0.805)
19. Zheng QL, Chen J, Nie ZM, Lv ZB, Wang D, Zhang YZ. Expression, purification and
characterization of a three-domain Kazal-type inhibitor from silkworm pupae (Bombyx mori).
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 2007
Feb, 146(2): 234-240. (SCI, IF: 1.404)
20. Zhang Y, Chen J, Lv Z, Nie Z, Zhang X, Wu X. Can 29kD rhGM-CSF expressed by
Silkworm pupae bioreactor bring into effect as active cytokine through Orally Administration?
European Journal of Pharmaceutical Sciences. 2006 Jun, 28(3): 212-223. (SCI, IF: 2.482)
21. Yu W, Chen J, Zhao XL. Expression of polyhedrin-hEGF fusion protein in cultured cells
and larvae of Bombyx mori. African Journal of Biotechnology. 2006; 5 (11): 1034~1040
(SCI).
22. 夏学春，陈健，张耀洲。 人乙肝病毒前表面抗原preS1在家蚕培养细胞中的表达。蚕
业科学，2005，31（1）：103-106。
联系方式
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通讯地址：杭州市下沙高教西区 2 号大街 5 号（310018）
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Email: chj1999@126.com
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手机：13093764932
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Tel: 0571-86843193