Trillium Lyse Concentrate
Material Safety Data Sheet
TRILLIUM DIAGNOSTICS
Revision Number: 1
Revised Date: 29 Jan, 2014
1. Identification of the substance/mixture and of the company
1.1 Product Identifier
Product Name:
Trillium Lyse Concentrate
Product Number:
LYS-30
1.2 Relevant identified uses of the substance or mixture and uses advised against
Trillium Lyse Concentrate is a 10X concentrate when diluted 1:10 with reagent grade water, is intended
for use for the lysis of red blood cells for the purpose of antibody labeling of white blood cells in whole
blood. Trillium Lyse Concentrate is also suitable for use in any laboratory application in which removal
of red blood cells from whole blood is necessary.
This product is intended for use as a General Laboratory Reagent. Not for in vivo use.
1.3 Details of the supplier of the safety data sheet
Manufacturer:
Trillium Diagnostics, LLC
Street/PO:
PO BOX 67
City, State, Postal Code,
Brewer, Maine, 04412, USA
Contact for Technical Inquiries:
Bruce H. Davis, info@trilliumdx.com
Phone, Fax, E-mail:
(207) 945-0900, (207) 942-0346, info@trilliumdx.com
1.4 Emergency telephone numbers
ITALY:
+39 06 305 4343 (Centro Antiveleni, Roma)
FRANCE:
+33 1 40 05 48 48 (Centres antipoison et de toxicovigilance, Paris)
GERMANY:
+49 551 19240 (Giftinformationszentrum-Nord, Göttingen)
NETHERLANDS:
+31 88 755 85 61 (National Poisons Information Centre, Utrecht)
SPAIN:
+34 91 562 04 20 (Servicio de Información Toxicológica, Instituto Nacional de
Toxicología, Madrid)
UNITED KINGDOM:
+44 (0)20 7188 0100 (Guy’s & St. Thomas’ Poisons Unit, Medical Toxicology
Services, London)
UNITED STATES
OF AMERICA:
+1 800 222 1222 (All US poison centers can be reached at this number)
See www.who.int/ipcs/poisons/centre/en/ for a worldwide directory of poison control centers.
Phone: 207-945-0900  Fax: 207-942-0346  info@trilliumdx.com  www.trilliumdx.com
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Trillium Lyse Concentrate
MATERIAL SAFETY DATA SHEET
2. Hazards identification
TRILLIUM DIAGNOSTICS
2.1 Classification of the substance or mixture
The product is not classified as hazardous according to Directive 1999/45/EC and Directive
67/548/EEC.
2.2 Label elements
The product composition does not contain dangerous substances in amounts that need to be
hazard labeled according to EC Directive 88/379/EEC.
R-Phrases:
None apply.
S- Phrases
S13
Keep away from food, drink, and animal feedstuffs.
S18
Handle and open container with care.
S20 /21 When using do not eat, drink or smoke.
S24 /25 Avoid contact with skin and eyes.
S26
In case of contact with eyes, rinse
S27/28
After contact with skin, wash with soap and water.
S35
This material and its container must be disposed of in a safe way
S36/37/39 Wear suitable protective clothing, gloves and eye/face protection.
2.3 Other Hazards
There are no reported health hazards for the product in the current formulation and applications.
The statements below refer to the hazard information known about the substances in their pure
forms. The product composition does not contain dangerous substances in amounts that need
to be hazard labeled according to EC Directive 88/379/EEC. No toxic effects are to be expected
when the product is handled appropriately.
Potential Health Effects

Ammonium Chloride (H4ClN)-Ammonium chloride may be harmful if ingested, inhaled, or
absorbed through the skin. It can cause serious irritation to the skin, respiratory tract and
especially the eyes. Use gloves when handling and avoid skin contact. Handle in
accordance with good laboratory practices using appropriate precautions. Avoid skin or eye
contact. Do not inhale or ingest.
 EDTA (Ethylenediaminetetraacetic acid) – EDTA may be harmful if ingested, inhaled, or
absorbed through the skin. Use gloves when handling and avoid skin contact. Handle in
accordance with good laboratory practices using appropriate precautions. Avoid skin or eye
contact. Do not inhale or ingest.
 Pottassium Phosphate (H2KO4P) – Potassium Phosphate may be harmful if ingested,
inhaled, or absorbed through the skin. It can cause serious irritation to the skin, respiratory
tract and especially the eyes. Use gloves when handling and avoid skin contact. Handle in
accordance with good laboratory practices using appropriate precautions. Avoid skin or eye
contact. Do not inhale or ingest.
Phone: 207-945-0900  Fax: 207-942-0346  info@trilliumdx.com  www.trilliumdx.com
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Trillium Lyse Concentrate
MATERIAL SAFETY DATA SHEET
Potential Chronic Health Effects:
 Carcinogenic Effects: None of the component substances are known to be carcinogenic.
 Mutagenic Effects: None of the component substances are known to be mutagenic..
TRILLIUM DIAGNOSTICS
3. Composition/information on ingredients
3.1 Substances
There are no reported or expected further health hazards for the product in the current
formulations and applications. The components of this product contain substances that may be
hazardous when available in significant amounts and should be treated as potentially
biohazardous.
3.2 Mixtures
Components:
Trillium Lyse Concentrate
Ammonium Chloride
EDTA
Potassium Phosphate
CAS-No.
EC-No.
Annex I Index-No
12125-02-9
60-00-4
7778-77-0
235-186-4 017-014-00-8
200-449-4 607-429-00-8
231-913-4 None
4. First aid measures
4.1 Description of first aid measures
After skin contact: Wash with plenty of water for 15 minutes. Remove contaminated
clothing. Seek medical advice if irritation develops.
After eye contact: Check for and remove contact lenses. Flush eye with plenty of water for
15 minutes and seek medical advice.
After Ingestion:
Rinse mouth with water for 15 minutes and seek medical advice. Do
NOT induce vomiting unless directed to do so by medical personnel.
After Inhalation:
Expose to fresh air. If breathing problems persist seek medical advice.
4.2 Most important symptoms and effects, both acute and delayed
Not available
4.3 Indication of any immediate medical attention and special treatment needed
See above under section 4.1
5. Firefighting measures
5.1 Extinguishing media
Carbon dioxide or dry chemical extinguishers or water are appropriate.
Small Fire: Use DRY chemical powder.
Large Fire: Use water spray, fog or foam. Do not use water jet.
Suitable extinguishing media include carbon dioxide, dry chemical extinguishers, or water.
5.2 Special hazards arising from the substance or mixture
Phone: 207-945-0900  Fax: 207-942-0346  info@trilliumdx.com  www.trilliumdx.com
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TRILLIUM DIAGNOSTICS
Trillium Lyse Concentrate
MATERIAL SAFETY DATA SHEET
Hazardous decomposition products formed under fire conditions – Oxides of phosphorous,
potassium oxides, carbon oxides, nitrogen oxides, and hydrogen chloride gas
The product itself is not combustible.
5.3 Advice for firefighters
This product does not cause special protective equipment to be required. In the event of a large
laboratory fire, wear full protective clothing and NIOSH-approved self-contained breathing
apparatus with full-face piece operated in the pressure demand or other positive pressure
mode. Water spray may be used to keep fire-exposed containers cool. Poisonous gases may
be produced in fires.
6. Accidental release measure
6.1 Personal precautions, protective equipment and emergency procedures
Use universal precautions, appropriate personal protective equipment and standard safe
laboratory practices to clean up spilled substance promptly. Absorb spill onto an appropriate
material. Consult local, state or federal regulations for more information regarding proper
disposal.
6.2 Environmental precautions
Do not let product enter drains. Avoid discharge into the environment.
6.3 Methods and material for containment and cleaning up
Soak up spills with an appropriate absorbent material. Consult local, state, or federal regulations
for proper disposal.
6.4 Reference to other sections
See Section 2.3, 7 and 8 for follow protective measures.
7. Handling and storage
7.1 Precautions for safe handling
Universal precautions should be followed when using this product. Avoid inhaling, ingesting
and contact with eyes and skin. Wash hands after handling. Wear appropriate personal
protective equipment and follow safe laboratory practices.
 No eating, drinking, or smoking in work areas
 Wear appropriate personal protective equipment
 Wash hands after use
 Remove contaminated clothing and protective equipment before leaving work area
 Avoid inhaling, ingesting, and contact with eyes and skin
7.2 Conditions for safe storage, including any incompatibilities
Store Trillium Lyse Concentrate vials upright, tightly capped at 2-8C when not in use. Avoid
unnecessary cycles of warming and cooling. Protect product from freezing, temperatures
above 30C, or prolonged exposure to light.
After dilution of the Trillium Lyse Concentrate, the diluted working solutions of the buffer should
be refrigerated (2-8C).
Phone: 207-945-0900  Fax: 207-942-0346  info@trilliumdx.com  www.trilliumdx.com
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MATERIAL SAFETY DATA SHEET
TRILLIUM DIAGNOSTICS
7.3 Specific end use(s)
Trillium Lyse Concentrate is intended for use for lysing red blood cells in preparations of whole
blood. Trillium Lyse concentrate can be used for removal of red blood cells prior to white blood
cell antibody labeling, or for other laboratory assays in which red blood cell removal from whole
blood is necessary. Universal precautions should be used.
8. Exposure controls/personal protection
8.1 Control parameters
The product does not contain any materials that need to be monitored at the workplace in
accordance with Directive 98/24/EC. Universal precautions should be followed when using this
product.
8.2 Exposure controls
Universal precautions should be used. Under normal conditions, the use of this product should
not require respiratory protection. Wear appropriate personal protective equipment and follow
safe laboratory practices.
Body protection:
None required
Eye protection:
Safety glasses according to EN 374
Skin protection:
Gloves according to EN 166
Respiratory protection:
None required when product is used as recommended
9. Physical and chemical properties
9.1 Information on basic physical and chemical properties
Trillium Lyse Concentrate
Appearance:
Clear, colorless liquid
Odor:
None
Odor Threshold:
Not Applicable
pH:
Neutral
Initial boiling Point:
Not Applicable
Melting point / freezing point:
Not available
Flash point:
Not Applicable
Evaporation Rate:
Not available
Flammability:
Not Applicable
Upper / Lower Flammability Limits: Not Applicable
Vapor Pressure:
Not Applicable
Vapor Density:
Not Applicable
Relative Density:
Not available
Solubility:
Fully miscible in water
Partition coefficient:
Not available
Auto-ignition temperature:
Product is not self-igniting
Decomposition Temperature:
Not Applicable
Viscosity:
Not available
Explosive properties:
Product is not explosive
Oxidizing properties:
Product is not oxidizing
Phone: 207-945-0900  Fax: 207-942-0346  info@trilliumdx.com  www.trilliumdx.com
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9.2 Other information
No other information available.
TRILLIUM DIAGNOSTICS
10. Stability and reactivity
10.1 Reactivity
Stable under normal temperatures and pressures.
10.2 Chemical stability
The product components in their final concentrations and volumes are stable and nonhazardous when properly handled.
10.3 Possibility of hazardous reactions
No hazardous incompatibilities identified
10.4 Conditions to avoid
None identified.
10.5 Incompatible materials
No hazardous incompatibilities identified.
10.6 Hazardous decomposition products
No hazardous decomposition products are known to be formed by this product.
11. Toxicological information
11.1 Information on toxicological effects
The complete toxicological effects of the product components in their final concentrations and
volumes have not been fully investigated and are expected to be non-hazardous when properly
handled. However, the product contains substances that may cause serious problems when
available in significant amounts. The product should be handled using universal precautions
and considered to be potentially infectious.
Acute Toxicity:
Skin Corrosion/Irritation:
Serious Eye Damage/Irritation:
Respiratory or Skin Sensitization:
Toxicity on Repeated Exposure:
Carcinogenity:
Mutagenicity:
Toxicity for Reproduction:
No toxic effect known.
No irritant effect known.
No irritant effect known.
No sensitizing effect known.
No toxic effect known.
No effect known.
No effect known.
No toxic effect known.
12. Ecological information
12.1 Toxicity
Undetermined.
Phone: 207-945-0900  Fax: 207-942-0346  info@trilliumdx.com  www.trilliumdx.com
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12.2 Persistence and degradability
Undetermined.
TRILLIUM DIAGNOSTICS
12.3 Bioaccummulative potential
Undetermined.
12.4 Mobility in soil
Undetermined.
12.5 Results of PBT and vPvB assessment
Undetermined.
12.6 Other adverse effects.
The complete ecological properties of the product components in their final concentrations and
volumes have not been investigated and are expected to be non-hazardous when properly
handled. However, the product contains substances that may cause serious problems when
available in significant amounts. No ecological problems are to be expected when the product is
handled and used with due care and attention.
13. Disposal considerations
13.1 Waste treatment methods
There are no uniform EC regulations for the disposal of chemicals or biohazardous waste.
Country, State, and local regulations are varied and change frequently, we recommend that you
contact either the authorities in charge or appropriate waste disposal companies which will
advise you on how to dispose of potentially infectious biological product waste. Dispose
contaminated packaging in compliance with local, state and federal regulations. Handle
contaminated packaging in the same manner as the substance itself.
14. Transport information
14.1 UN Number
Not applicable.
14.2 UN proper shipping name
Not determined.
14.3 Transport hazard class
Not classified as hazardous under RID/ADR regulations (Ref. UN TRANS/WP.15/AC.1/94
Add.2 Sec.2.2.62.1.6 and 2.2.62.1.7)
14.4 Packing group
Not applicable.
14.5 Environmental hazards
Not applicable.
Phone: 207-945-0900  Fax: 207-942-0346  info@trilliumdx.com  www.trilliumdx.com
LYS MSDS LTR v1 2014
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14.6 Special precautions for users
See subsections 6-8.
TRILLIUM DIAGNOSTICS
14.7 Transport in bulk according to Annex II of MARPOL73/78 and the IBC Code
Not classified.
15. Regulatory Information
15.1 Safety, health and environmental regulations specific for the substance or mixture
Product is not classified. To the best of our knowledge, safety, health, and environmental
regulations according to VO (EC) 1907/2006 are not applicable.
15.2 Chemical safety assessment
No chemical safety assessment has been carried out.
16. Other Information
Literature References
1. “Biosafety in Microbial and Biomedical Laboratories”, 2nd ed.,1988. HHS Publication No.
88-8395, Centers for Disease Control.
2. EC Directives 1999/45/EC, 67/548/EEC, 89/686/EE and 88/379/EEC
3. 29.CFR1910.1030, Occupational Exposure to Bloodborne Pathogens
4. CEN Standards, in accordance with EC Directive 98/24/EC
Notes
The information presented in this Material Safety Data Sheet is based on the present state of
our knowledge. The product should be used according to the instructions provided by the
manufacturer, see “Instructions for use” as presented in the package insert accompanying every
product. We make no warranty, express or implied, with respect to such information, and we
assume no liability resulting from its use. Users should make their own investigations to
determine the suitability of the information for their particular purposes. The product should be
used according to the instructions provided by the manufacturer, see “instructions for use” as
presented in the Package Insert accompanying every product. Trillium Diagnostics, LLC and
distributors thereof shall not be held liable for any claims, losses, or damages resulting from
handling or from contact with the product. For additional information, see our website,
www.trilliumdx.com.
Phone: 207-945-0900  Fax: 207-942-0346  info@trilliumdx.com  www.trilliumdx.com
LYS MSDS LTR v1 2014
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