Hellenic Accreditation System
Annex F1/Α15 to the Certificate No. 152-3
SCOPE of ACCREDITATION
of the
Laboratory of Food of Animal Origin Control
of the
Veterinary Services of the Ministry of Agriculture
of the
Natural Resources and Environment of Cyprus
Materials/Products tested
Types of test/Properties measured
Applied methods/Techniques used
Microbiological Testing
1. Fresh meat
1.Screening for the detection of
antibacterial substances
Four Plate Test (FPT) (SOP 29),
96/23/EEC: 1996
Veterinary Drug Residues, 2nd
Ed.:1994
Report Eur 15127-EN
2.Fresh meat and fish
1. Biological screening method for the
detection of antimicrobial substances
Premi-Test (r-biopharm): Standard
diffusion test (SOP 145) for the
detection of antibacterial substances
in fresh meat and fish
3. Meat
1. Detection of Trichinella
Commission Regulation (EC) No
2075/2005, Annex I: Detection
methods, Chapter I: Reference
Method of Detection (Magnetic
stirrer method for pooled sample
digestion) (SOP 121)
4. Food of animal origin
and animal feeding stuffs
1.Detection of Salmonella spp.
ISO 6579:2002
2.Detection of Listeria monocytogenes
ISO 11290-1:1996/Amd 1:2004
3. Enumeration of Campylobacter spp.
ΕN ISO 10272-2:2006
1.Enumeration of colony forming units
of micro-organisms at 30 oC
ISO 4833-1:2013
2. Biological screening method for the
detection of antimicrobial substances
DSM Delvotest SP-NT: Standard
diffusion test (SOP 63) for the
detection of antibacterial substances
in milk
5. Milk
Page 1 of 4
Annexes F1/Α15 and B4 to the Certificate ESYD No.152-3
02.07.2015
Materials/Products tested
6. Raw milk
7. Milk and milk products
8. Food of animal origin
9. All food matrices
Types of test/Properties measured
Applied methods/Techniques used
1.Enumeration of somatic cells
ISO 13366-1:2008 (Microscopic
method)
2.Enumeration of somatic cells
ISO 13366-2:2006 (Fluoro-optoelectronic method)
1.Enumeration of coagulase positive
staphylococci
ISO 6888-2:1999 /Amd 1:2003
2.Enumeration of β-glycuronidase
positive Escherichia coli
ISO 16649-2:2001
3. Enumeration of Enterobacteriaceae
ISO 21528-2:2004
1.Enumeration of Listeria
monocytogenes
ISO 11290-2:1998/Amd 1:2004
2.Detection of Campylobacter spp.
ISO 10272-1:2006
3. Detection of Escherichia coli O157
ISO 16654:2001
1. Detection of staphylococcal
enterotoxins
European screening method (SOP
99) of the CRL- Coagulase positive
Staphylococci, including
Staphylococcus aureus.
“Detection of Staphylococcal
enterotoxins types SEA to SEE in
all food matrices”, Version 5,
September 2010.
10. Salmonella isolates
1.Serotyping of Salmonella spp.
White Kauffmann Le Minor
Scheme_2007, 9th edition (SOP
147)
Chemical Testing
1. Milk and milk powder
1. Determination of aflatoxin M1
content
ISO 14501:2007
Determination of aflatoxin M1
content – Clean-up by
immunoaffinity chromatography
and determination by highperformance liquid chromatography
(SOP 142)
2. Milk, milk powder,
cheese and yoghurt
1. Biochemical screening method for the Quantitative enzyme immunoassaydetection and quantification of Aflatoxin ELISA Afla M1, Tecna, code
M1
MA418 (SOP 73)
Site of assessement: Permanent Laboratory premises, Veterinary Services, Athalassa, Nicosia, Cyprus.
Approved signatories: P. Styllianou, M. Emmanuel, C. Arsenoglou, E. Papageorgiou.
This Scope of Accreditation replaces the previous one dated 26.03.2015.
The Accreditation Certificate No.152-3, to ELOT EN ISO/IEC 17025:2005, has been expanded until 02.03.2016.
Athens, July 02, 2015
Ioannis Sitaras
Director of the Laboratories Accreditation Division
Page 2 of 4
Annexes F1/Α15 and B4 to the Certificate ESYD No.152-3
02.07.2015
Hellenic Accreditation System
Annex F1/B4 to the Certificate No. 152-3
SCOPE of ACCREDITATION
of the
Laboratory of Food of Animal Origin Control
of the
Veterinary Services of the Ministry of Agriculture
of the
Natural Resources and Environment of Cyprus
Tested materials and products
Types of test/ Properties to be
measured
Applied standards/ Techniques to be used
Chemical Tests
1. Raw and pasteurized cow
milk
1.Determination of total solids
content
ISO 6731:2010 (IDF 21:2010)
2. Determination of fat
ISO 1211:2010 (IDF 1:2010)
Gravimetric οf Dry Matter - Reference method
Extraction Gravimetric (Rose Gottlieb) Reference method
3. Determination of Fat, Protein
and Lactose
Routine method, MidIR Spectroscopy
(FTIR/Fourier Transformation Infrared) with the
instrument FOSS FTPlus based on the Guidance
of Infrared Spectroscopy Methods IDF 141C:
2000
4. Determination of Lactose
ISO 22662:2007 (IDF 198:2007)
High Performance Liquid Chromatography
(HPLC)- Reference method
5. Determination of Nitrogen and
calculation of Protein
6. Determination of Aflatoxin M1
ISO/TS 17837:2008 (IDF/RM 25:2008)
Kjeldahl /Block digestion method
ΙSO 14675:2003
Quantitative enzymatic method of
Immunoabsorbance (Screening method) ELISA Aflatoxin M1 Tecna, code MA418
(SOP 73)
Physical Tests
1. Raw and pasteurized milk
Page 3 of 4
1. Determination of freezing point
(GE – moC)
ISO 5764 (IDF 108:2009) - Thermistor
Cryoscopy – Reference method
Annexes F1/Α15 and B4 to the Certificate ESYD No.152-3
02.07.2015
Tested materials and products
Types of test/ Properties to be
measured
Applied standards/ Techniques to be used
Microbiological Tests
1. Raw milk
1.Enumeration of microorganisms
at 30 oC
ISO 4833:2013
2.Detection of antibacterial
substances-Brilliant Reduction
test
In house method based on ISO 13969:2003
(DELVOTEST SP-NT) (SOP 300)
3.Enumeration of somatic cells
ISO 13366-2:2006 (IDF 148-2: 2006)
SPC at 30oC – Reference method
Routine method, Fluoro-opto-electronic Cells
counting – Flowing Cytometry method, with the
instrument FOSS FC
2. Raw milk
1. Bacteria enumeration
Routine method, Fluoro-opto-electronic bacteria
counting - Flowing Cytometry method, with the
instrument IBC BENTLEY-BACTOCOUNT.
ISO 16297:2013
Site of assessment: Permanent laboratory premises, 6 Amphipoleos str, Nicosia, Cyrpus.
Approved signatories: P. Styllianou, M. Emmanuel, C. Arsenoglou, E. Papageorgiou.
This Scope of Accreditation replaces the previous one dated 26.03.2015.
The Accreditation Certificate No.152-3, to ELOT EN ISO/IEC 17025:2005, has been expanded until 02.03.2016.
Athens, July 02, 2015
Ioannis Sitaras
Director of the Laboratories Accreditation Division
Page 4 of 4
Annexes F1/Α15 and B4 to the Certificate ESYD No.152-3
02.07.2015