1
FINAL PROGRAMME
Joint
British Society for Haemostasis & Thrombosis
&
UK Platelet Group
Annual Scientific and general meeting
Assembly Rooms, Bath
18th-19th October 2012
THURSDAY 18th October
09.00
Registration opens & Coffee
10:00 – 12:10 SESSION 1 – Chairpersons – Paul Harrison & Steve Watson
10.00 – 10.40
NOVEL PHOSPHOLIPIDS AND PLATELET PROCOAGULANT ACTIVITY
Dr. Peter Collins, University Hospital of Wales, Cardiff, UK
10.40 – 10.55
O1
TRANSIENT
RECEPTOR
POTENTIAL
CHANNELS
(TRPCs)
CONTRIBUTE TO PLATELET PHOSPHATIDYLSERINE EXPOSURE
Matthew T. Harper, Juan E. C. Londoño, Kathryn Quick, Lutz Birnbaumer, Marc
Friechel, Alastair W. Poole. School of Physiology and Pharmacology, University of
Bristol, Bristol. Department of Experimental and Clinical Pharmacology and
Toxicology, Faculty of Medicine, Saarland University, Homburg, Germany. Molecular
Nociception Group, Wolfson Institute for Biomedical Research, University College
London, London.
10.55 – 11.10
O2
MEGAKARYOCYTE-SPECIFIC DELETION OF THE PROTEIN-TYROSINE
PHOSPHATASES SHP1 AND SHP2 CAUSES A BLOCK IN MEGAKARYOCYTE
DEVELOPMENT AND SEVERE MACROTHROMBOCYTOPENIA
Alexandra Mazharian, Jun Mori, Ying-Jie Wang, Silke Heising, Benjamin G. Neel,
Steve P. Watson and Yotis A. Senis. Centre for Cardiovascular Sciences, School of
Clinical and Experimental Medicine, Institute of Biomedical Research, University of
Birmingham, Birmingham, UK. Weatherall Institute of Molecular Medicine, University
of Oxford, John Radcliffe Hospital, Headington, Oxford, UK. Campbell Family Cancer
Research Institute, Ontario Cancer Institute, Princess Margaret Hospital, University
Health Network and Department of Medical Biophysics, University of Toronto,
Toronto, Ontario, Canada
2
11.10 – 11.25
O3
P2Y12 CONTROLS A MUNC13-4-INDEPENDENT PATHWAY TO
PLATELET SECRETION OF α-GRANULES BUT NOT DENSE GRANULES
Matthew T. Harper, Joshua S. Savage, Christopher M. Williams & Alastair W. Poole
School of Physiology and Pharmacology, Medical Sciences Building, University of
Bristol, Bristol, BS8 1TD, UK.
11.25 – 11.40
O4
A NOVEL MUTATION IN THE DRY MOTIF OF THE P2Y12 RECEPTOR
RESULTS IN CHRONIC BLEEDING IN A PATIENT Stuart Mundell, Kathryn
Garner, Shaista Nisar, Steve Watson, Yatin Patel, Steve Austin on behalf of the UK
GAPP Study Group School of Physiology and Pharmacology. University of Bristol,
University Walk, Bristol, BS8 1TD, UK, School of Clinical and Experimental Medicine
Institute of Biomedical Research, University of Birmingham, Birmingham B15 2TT,
UK, Kings College London, Rayne Institute, St. Thomas' Hospital, London, UK,
4Centre for Haemostasis and Thrombosis, St. Thomas' Hospital, London, UK
11.40 – 11.55
O5
PROCOAGULANT MICROPARTICLES DURING CHEMOTHERAPY FOR
NON-HODGKIN LYMPHOMA Sunna Asghar, Henry Watson, Paraskevi Untiveros,
Dominic Culligan, and Isobel Ford. Cardiovascular Research Programme, School of
Medicine & Dentistry, University of Aberdeen and Department of Haematology,
Aberdeen Royal Infirmary, Aberdeen, Scotland.
11.55 – 12.10
O6
CHARACTERISATION OF THE PROCOAGULANT PROPERTIES OF
MICROPARTICLES DERIVED FROM DIFFERENT CELL TYPES Mohammed A
Alsahli, Hassan S Hamali, Alison H Goodall. Department of Cardiovascular
Sciences, University of Leicester, UK, and College of Applied Medical Sciences, King
Khalid University, Abha, Kingdom of Saudi Arabia
12.10 – 12.40
BSHT AGM – BSHT members only
12.40 – 14.00
LUNCH, EXHIBITION & coffee/tea & poster viewing
14.00 – 15.40 SESSION 2 – Chairpersons – Nicola Mutch and Robert Ariens
14.00 – 14.40
PLATELET POLYPHOSPHATE AND THE CONTACT SYSTEM
Professor Jim Morrissey, Department of Biochemistry, College of Medicine,
University of Illinois, USA
14.40 –14.55
O7
IMMOBILIZED TRANSITION METALS STIMULATE ACTIVATION OF THE
CONTACT PATHWAY Nicola J Mutch, Emily K Waters, Jim H Morrissey. Institute of
Medical Sciences, University of Aberdeen, UK; Department of Biochemistry,
University of Illinois, Urbana, IL, USA
14.55 – 15.10
O8
FLUORESCENT CONFOCAL MICROSCOPY AS A TOOL TO TRACK AND
INVESTIGATE FIBRINOLYSIS Claire Whyte, and Nicola Mutch, Institute of Medical
Sciences, University of Aberdeen, Aberdeen, UK
15.10 – 15.25
O9
IDENTIFICATION OF NOVEL VARIATIONS IN PLATELET GPCRS IN
PATIENTS HISTORICALLY DIAGNOSED WITH TYPE 1 VON WILLEBRAND
DISEASE IN THE EUROPEAN MCMDM-1VWD STUDY J. Stockley, A. Goodeve, S.
Nisar, A.D. Mumford, S.J. Mundell, S.P. Watson and M.E. Daly on behalf of the UK
GAPP Study Group and in collaboration with the EU MCMDM-1VWD study group.
Department of Cardiovascular Science, University of Sheffield, Sheffield, S10 2RX;
3
School of Physiology and Pharmacology, University of Bristol, Bristol, BS8 1TD;
Bristol Heart Institute & School of Cellular and Molecular Medicine, University of
Bristol, Bristol, BS2 8HW; Centre for Cardiovascular Sciences, College of Medical
and Dental Sciences, University of Birmingham, Birmingham, B15 2TT
15.25 –15.40
O10
THE TOLL-LIKE RECEPTOR 2 LIGAND PAM3CSK4 INDUCES PLATELET
ACTIVATION VIA THE SRC/SYK/LAT/PLC2 SIGNALLING PATHWAY. Knut
Fälker & Magnus Grenegård. Linköping University, Faculty of Health Sciences,
Department of Medicine and Health, Division of Drug Research/Pharmacology, and
Department of Clinical and Experimental Medicine, Division of Clinical Chemistry,
58185 Linköping, Sweden
15.40 – 16.10
Coffee Break and EXHIBITION
16.10 – 17.35
SESSION 3 Chairpersons Alison Goodall & Jon Gibbins
16.10 – 16.50
NEW ADVANCES IN ANTIPLATELET AGENTS Dr. Azfar Zaman. Freeman
Hospital, Newcastle UK
16.50 – 17.05
O11
WOULD A NEW FOCUS ON VASCULAR PROSTAGLANDINS IMPROVE
ANTIPLATELET TREATMENT REGIMES? Stan Heptinstall, David Iyú, Jacqueline
R. Glenn, Ann E. White, Sue Fox. Cardiovascular Medicine, University of Nottingham,
Nottingham, UK
17.05 – 17.20
O12
A SUBPOPULATION OF UNINHIBITED PLATELETS CAN PROVIDE THE
FOCUS FOR PLATELET AGGREGATE FORMATION Thomas Hoefer, & Timothy
D. Warner, William Harvey Research Institute, Barts and the London School of
Medicine and Dentistry, Queen Mary University of London
17.20 – 17.35
O13
CROSS REGULATION OF THROMBOXANE A2 (TP) AND P2Y
RECEPTORS IN HUMAN PLATELETS M.I. Butler, R.J. P. Pope, D.A. Marsden, J.F.
Barton, A. D. Mumford, A. W. Poole, T. W. Johnson, and S.J. Mundell. School of
Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom, Bristol
Heart Institute, University of Bristol, Bristol Royal Infirmary, Bristol, United Kingdom
17.35 - 18.35
Poster viewing (authors to be present) & Drinks
19:15
Drinks reception at the Roman Baths
20:00
Conference Dinner in the Pump Room
4
FRIDAY 19th October
9.00 - 11.10
SESSION 4 Chairpersons Jim Crawley & Anne Goodeve
9.00 - 9.40
TARGETING COMPLEMENT IN AYTYPICAL HAEMOLYTIC
SYNDROME. Dr. Daniel Gale, University College London, UK.
9.40 - 9.55
O14
DOMAIN SPECIFICITY OF ANTI-ADAMTS13 ANTIBODIES AT
PRESENTATION AND RELAPSE IN THROMBOTIC THROMBOCYTOPENIC
PURPURA (TTP) Mari Thomas, Marie Scully, Samuel Machin, James Crawley.
Haemostasis Research Unit, Department of Haematology, University College
London, UK. Department of Haematology, University College London Hospital, UK.
Centre for Haematology, Imperial College London, UK
9.55 - 10.10
O15
ANALYSIS OF PROTEIN DISULPHIDE ISOMERASE MOTIFS (CXXC) IN
MATURE VON WILLEBRAND FACTOR. Susie Shapiro, Mike Laffan, Thomas,
McKinnon. Centre for Haematology, Division of Experimental Medicine, Department
of Medicine and Faculty of Medicine, Imperial College London, London, W12 0NN,
United Kingdom
10.10 – 10.25
O16
IDENTIFICATION, CHARACTERISATION AND IN SILICO ANALYSIS OF
DELETION BREAKPOINTS IN THE VWF GENE FROM THE EU MCMDM-1VWD
COHORT Ashley Cartwright, Daniel Hampshire, Lisa Bloomer, Ian Peake and Anne
Goodeve on behalf of the EU-VWD study. Haemostasis Research Group, University
of Sheffield, Sheffield, UK
10.25 - 10.40
O17
DIRECT BINDING BETWEEN PROTEIN S AND TFPI IS ESSENTIAL FOR
PROTEIN S TO ACT AS A COFACTOR FOR TFPI Josefin Ahnström*, Helena M
Andersson*, James TB Crawley, David A Lane. *Authors contributed equally .Centre
for Haematology, Division of Experimental Medicine, Imperial College London
10.40 - 11.10
EXHIBITION & coffee & tea
URAEMIC
11.10 – 13.05 Session 5 Chairpersons Alastair Poole & Chris Gardiner
11.40 – 12.20
FACTOR VIIa AND PLATELETS. Professor Philip de Groot, University Medical
Center, Utrecht. The Netherlands
12.20 – 12:35
O18
THE INTERACTION OF TISSUE FACTOR WITH FILAMIN-A IS
ESSENTIAL FOR THE RELEASE OF TISSUE FACTOR INTO ENDOTHELIAL
CELL MICROPARTICLES Mary Collier, Anthony Maraveyas, Camille Ettelaie.
Biomedical Section, Department of Biological Sciences and Division of Cancer,
Postgraduate Medical Institute in association with Hull York Medical School,
University of Hull, Cottingham Road, Hull, UK.
12.35 – 12:50
O19
A PROCOAGULANT TIME-BOMB ON ENDOTHELIAL CELLS: THE
UPTAKE OF MICROPARTICLES AND RECYCLING OF THE TISSUE FACTOR
COMPONENT Camille Ettelaie, Pui Mei Mah, Anthony Maraveyas, Mary EW Collier.
Biomedical Section, Department of Biological Sciences and Division of Cancer,
Postgraduate Medical Institute in association with Hull York Medical School,
University of Hull, Cottingham Road, Hull, UK.
5
12.50 – 13.05
O20
WHEN IS AN ‘ACTIVATED’ PLATELET ACTIVATED? Emily C. Reddy,
Robert J. Forster, Sarah O’Neill. School of Chemical Sciences, National Centre for
Sensor Research, Dublin City University, Dublin 9, Ireland. Molecular and Cellular
Therapeutics, Royal College of Surgeons in Ireland, Dublin 2, Ireland.
13.05 - 14.10
LUNCH EXHIBITION & coffee & tea, registration
14.10 – 16.45 Session 6 Chairpersons – Paul Harrison & Henry Watson
14.10 – 14.40
PROTEIN DISULPHIDE ISOMERASES AND HAEMOSTASIS
Professor Jon Gibbins, University of Reading, UK
14.40 – 16.40
Scientists in Training best scoring abstracts
14.40 – 14.55
O21
ASPIRIN INHIBITS THE PLATELET-MEDIATED EXPRESSION OF
ANTITHROMBOTIC GENES IN MONOCYTES Robert E Turnbull, Julian van
Capelleveen, Unni Krishnan, Joy R Wright, Nilesh J Samani, Mieke Trip, Suthesh
Sivapalaratnam, Alison H Goodall,
Department of Cardiovascular Sciences,
University of Leicester, UK and Department of Vascular Medicine, Amsterdam Medical
Centre, University of Amsterdam, The Netherlands
14.55 – 15.10
O22
OXIDIZED
LOW-DENSITY
LIPOPROTEINS
INDUCE
PLATELET
ACTIVATION AND SHAPE CHANGE THROUGH A NOVEL CD36-RHO KINASE
DEPENDENT SIGNALLING PATHWAY. Katie S Wraith, Simbarashe Magwenzi,
Ahmed Aburima, Yichuan Wen, David Leake and Khalid M Naseem. 1 Centre for
Cardiovascular and Metabolic Research, Daisy Building (Research Laboratories),
Hull York Medical School, Castle Hill Hospital (Entrance 2), Castle Road, Hull. HU16
5JQ. 2 School of Biological Sciences, University of Reading, Reading UK
15.10 – 15.25
O23
REGULATION OF INTEGRIN CLOSURE IN PROCOAGULANT
PLATELETS Nadine J.A. Mattheij, Karen Gilio, Roger van Kruchten, Shawn M
Jobe, Xiaoping Du, Adam J. Wieschhaus, Athar H. Chishti, Peter Collins, Johan W.M.
Heemskerk, Judith M.E.M. Cosemans. Department of Biochemistry, Cardiovascular
Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The
Netherlands. Department of Pediatrics, Emory University, Atlanta, GA, USA.
Department of Pharmacology, University of Illinois, Chicago, USA. Department of
Molecular Physiology and Pharmacology Tufts University School of Medicine, Boston,
MA, USA. Arthur Bloom Haemophilia Centre, Department of Haematology, Medical
School of Cardiff University, University Hospital of Wales, Cardiff, UK.
15.25 – 15.40
O24
THE C DOMAINS AND CERTAIN PREDICTED UNPAIRED CYSTEINES
OF VON WILLEBRAND FACTOR ARE ESSENTAL FOR ITS SECRETION Susie
Shapiro, Mike Laffan, Thomas McKinnon. Centre for Haematology, Division of
Experimental Medicine, Department of Medicine and Faculty of Medicine, Imperial
College London, London, W12 0NN, United Kingdom
15.40 – 15.55
O25
THE VWF A2 DOMAIN VICINAL C1669-C1670 DISULPHIDE BOND AND
OCCUPANCY OF CALCIUM GOVERN DISTINCT STEPS IN A2 DOMAIN
UNFOLDING Christopher Lynch, David A. Lane, Brenda M. Luken. Centre for
Haematology, Department of Medicine, Imperial College London
15.55 – 16.10
O26
ASPIRIN ATTENUTES PAI-1 EXPRESSION WI THIN
AN
ARTERIAL
THROMBUS Sameer Kurmani, Robert E Turnbull, Danny Chan, Alison H
6
Goodall. Department of Cardiovascular Sciences, University of Leicester, Glenfield
Hospital, Leicester, UK.
16:10 – 16.25
O27
THE EFFECT OF ADAMTS13 MUTATIONS ON ITS SECRETION,
ACTIVITY AND SUBCELLULAR LOCATION Mary Underwood, I. Garagiola, I.
Mackie, S. Machin & F. Peyvandi. Haemostasis Research Unit, University College
London, UK and Angelo Bianchi Bonomi Hemophilia and Thrombosis Centre,
University of Milan
16.25 – 16.40
O28
TARGETING OF TYPE I PROTEIN KINASE A TO LIPID RAFTS IS
REQUIRED FOR COMPETENT PLATELET INHIBITION BY THE CAMPSIGNALING PATHWAY. Zaher Raslan, Ahmed Aburima, Simbareshe Magwenzi,
Kjetil Tasken and Khalid M Naseem. Centre for Cardiovascular and Metabolic
Research, Daisy Building (Research Laboratories), Hull York Medical School, Castle
Hill Hospital (Entrance 2), Castle Road, Hull, UK. Biotechnology Centre of Oslo,
University of Oslo, Blindern, Oslo, Norway
16.45
Close of Conference
7
POSTERS
P1
2-ACETYLPHENOTHIAZINE INHIBITS SUPEROXIDE ION GENERATION IN HUMAN PLATELETS
AND IMPAIRS COLLAGEN-DEPENDENT THROMBUS FORMATION
D.S. Vara (1), M. Campanella (2,3), G. Pula (1). (1), Department of Pharmacy and Pharmacology,
University of Bath (UK); (2), Department of Veterinary Basic Sciences, Royal Veterinary College,
University of London (UK); (3), Consortium for Mitochondrial Research (CfMR), University College
London (UK).
P2
CHANGES IN PLATELET FUNCTION DURING STORAGE – EVALUATION OF A FLOW
CYTOMETRY METHOD FOR STUDIES OF PLATELET ADHESION AND ACTIVATION
Anna Södergren1, Nahreen Tynngård2, Sofia Ramström 1. 1Dept. of Clinical and Experimental
Medicine, Clinical Chemistry, Faculty of Health Sciences, Linköping University, S-581 85 Linköping,
Sweden. 2 Dept. of Clinical and Experimental Medicine, Transfusion Medicine, Faculty of Health
Sciences, Linköping University, S-581 85 Linköping, Sweden.
P3
REGULATION OF RHOA ACTIVATION BY CYCLIC AMP DEPENDENT KINASE SIGNALLING
A. Aburima, K. Wraith and K.M. Naseem. Centre for Cardiovascular and Metabolic Research, Hull
York Medical School, University of Hull, Hull.
P4
OXIDISED LDL ACTIVATES PLATELETS NADPH OXIDASE-DEPENDENT INHIBITION OF THE
CGMP/PROTEIN KINASE G SIGNALLING CASCADE.
Simbarashe Magwenzi, Catriona McNeil, Katie Wraith & Khalid Naseem. Centre for Cardiovascular
and Metabolic Research, Hull York Medical School, University of Hull, Hull.
P5
A ROLE FOR HISTONE DEACETYLASES IN THE REGULATION OF PLATELET FUNCTION
Marfoua S. Ali , Sakthivel Vaiyapuri, Chris I Jones, Jonathan M. Gibbins PhD and Michael J. Fry PhD
Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of
Reading, Reading, United Kingdom
P6
LARGE-SCALE SCREENING FOR EXTRACELLULAR PROTEIN INTERACTIONS INVOLVED IN
PLATELET AGGREGATION
Yi Sun & Gavin J. Wright. Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute,
Hinxton, Cambridge CB10 1HH, United Kingdom
P7
THE POOL NORM: PERFORMANCES TOWARDS APTT AND FACTOR LEVELS WITH STAGO
REAGENTS
L. Loï, F. Nicham, K. Berrendonner, K. Carrière. Diagnostica Stago (France)
P8
A NOVEL PLATELET FUNCTION ASSAY THAT IDENTIFIES BLEEDING RISK AND QUANTIFIES
ANTI-PLATELET DRUG EFFECT
A. Lopez-Alonso*, L. Basabe-Desmonts Ϯ, S. Ramstrom Ϯ, B. Jose Ϯ, M. Sommers Ϯ, L. Redahan¥, P.
Conlon¥, D. Kenny* Ϯ *NBIPI Program, Molecular & Cellular Therapeutics, Royal College of Surgeons
in Ireland (RCSI), Dublin, IRELAND, Ϯ Biomedical Diagnostics Institute (BDI), Dublin City University,
Dublin, IRELAND ¥Beaumont Hospital, Nephrology & Renal Transplant Medicine, Dublin IRELAND
8
P9
DOES CITALOPRAM INHIBIT COLLAGEN-INDUCED PLATELET AGGREGATION?
Richard D A Wilkinson1 & Gavin E Jarvis2, 1 School of Pharmacy, Queen’s University Belfast, 97
Lisburn Road, Belfast BT9 7BL, 2 Department of Physiology, Development & Neuroscience, University
of Cambridge, Downing Street, Cambridge CB2 3EG
P10
ADMINISTRATION OF PROTHROMBIN COMPLEX CONCENTRATES FOR WARFARIN
REVERSAL – ARE WE GETTING IT RIGHT?
Sarah Horton, Rachel Heath, Anthony Houghton, Vanessa Martlew, Jecko Thachil
Department of Haematology, Royal Liverpool University Hospital, Liverpool, UK. L7 8XP.
P11
Regulation of mouse platelet GPCR receptor function by NHERF proteins
Robert J P Pope, Shaista P Nisar, Margaret R Cunningham, Alastair W Poole & Stuart J Mundell.
School of Physiology and Pharmacology, Medical Sciences Building, University of Bristol, Bristol, BS8
1TD
P12
particulate matter: surface modification, size and thrombotic risk.
Smyth E1, Solomon A1, Thorley A2, Tetley T2, Emerson M1 1 Platelet Biology Group and 2Lung Cell
Biology Group, National Heart and Lung Institute, Imperial College London, London SW7 2AZ, UK.
P13
THE ANTI-PLATELET EFFECT OF SILDENAFIL IS DEPENDENT ON NITRIC OXIDE: FROM
WHERE?
Georgina Apostoli and Michael Emerson. Platelet Biology Group, Molecular Medicine, National Heart
and Lung Institute, Imperial College London, London SW7 2AZ.
P14
NANOPARTICLE INDUCED PLATLET AGGREGATION AND VASULAR THROMBOSIS: ROLE OF
DIESEL EXHAUST
1Solomon, A., 1Smyth, E., 1Meetha, N., 2Pitchford, S., 1Luther, P., 1Thorley, A., 1Tetley, T., and
1Michael Emerson. 1Platelet Biology Group, Molecular Medicine Section, National Heart and Lung
Institute, Imperial College London, London, UK. 2Institute of Pharmaceutical Science, Kings College
London, London, UK
P15
A HETEROZYGOTE MISSENSE MUTATION IN A PATIENT WITH GLANZMANN
THROMBASTHENIA LOCATED IN THE EXTRACELLULAR DOMAIN OF GPIIb.
Edwina Dobbin, Gillian McGaffin, Anne White, Wendy Hannant and David Stirling
Haematology Department, Edinburgh Royal Infirmary, 51 Little France Crescent, Edinburgh, EH16
4SA
P16
FACTOR XIII DEFICIENCY: MUTATIONS IN THE FACTOR A SUBUNIT (FX13A1) AND FACTOR B
SUBUNIT (FX13B) GENES.
Edwina Dobbin, Gillian McGaffin, Anne White, Wendy Hannant and David Stirling. Haematology
Department, Edinburgh Royal Infirmary, 51 Little France Crescent, Edinburgh, EH16 4SA
P17
CURRENT TRENDS IN THE USE OF INFERIOR VENACAVA FILTERS
Megan Kell, Richard McWilliams, Vanessa Martlew, Jecko Thachil
Department of Haematology and Radiology, Royal Liverpool University Hospital, Liverpool, UK. L7
8XP.
9
P18
RELATIVE ROLES OF PLATELET-DERIVED THROMBOXANE A2 AND 12-HPETE/HETE IN THE
REGULATION OF PLATELET AGGREGATION AND ADHESION
1Francesca Rauzi, 1,2Nicholas Kirkby, 2Jane A. Mitchell & 1Timothy D. Warner. 1The William Harvey
Research Institute, Barts & the London School of Medicine & Dentistry, Charterhouse Square, London
EC1M 6BQ; 2National Heart & Lung Institute, Imperial College, Dovehouse Street, London SW3 6LY.
P19
EVALUATION OF THE EFFECTS OF DABIGATRAN ON SPECIALIST COAGULATION ASSASYS.
Johnson AE, Kitchen S, Maclean R. Coagulation Department, Royal Hallamshire Hospital, Sheffield,
Glossop Road, S10 2JF.
P20
EVALUATION OF THE EFFECTS OF DABIGATRAN ON ROUTINE COAGULATION TESTS.
Johnson AE, Kitchen S, Maclean R. Coagulation Department, Royal Hallamshire Hospital, Sheffield,
Glossop Road, S10 2JF.
P21
EXTRACORPOREAL MEMBRANE OXYGENATION (ECMO) INDUCES NEUTROPHILS TO
RELEASE INTRACELLULAR DNA LEADING TO HAEMOSTASIS ACTIVATION.
Grace Britton1, Simon Davidson2, Sharon Mumby1, Mark Griffiths1, Gregory Quinlan1. Department of
Critical Care Medicine, Royal Brompton Hospital and National Heart and Lung Institute, Imperial
College, London, UK1, Department of Haematology, Royal Brompton Hospital, London, UK 2
P22
ESTABLISHING A CELL BASED ASSAY OF FIBRINOLYSIS
Sarah Mangles, Carolyn Millar and Mike Laffan. Imperial College, London
P23
SAFETY AND EFFICACY OF FACTOR XI CONCENTRATE FOR COVERING SURGERY IN
PATIENTS WITH FACTOR XI DEFICIENCY
Huseini Kagdi, Pratima Chowdary and Keith Gomez. Katherine Dormandy Haemophilia Centre and
Thrombosis Unit, Royal Free Campus, University College London Medical School
P24
DIAGNOSIS OF HEPARIN-INDUCED THROMBOCYTOPENIA USING THE HITAlert ASSAY
Bhavesh M Popat1, Chris Watson2, Kevin Horner3, Barbara Hopkins2, Simon Davidson4, Steve
Kitchen3, Joost J van Veen3, Sue Pavord2, Gillian Swallow2, Alison H Goodall1. 1Department of
Cardiovascular Sciences, University of Leicester, 2Department of Haematology, Leicester Royal
Infirmary, 3Department of Haematology, Sheffield Hallam Hospital, Sheffield and 4Royal Brompton
Hospital, London, UK.
P25
ASSESSMENT OF A NEW CHROMOGENIC FIX ASSAY.
Leeson KJ, Bowyer AE, Kitchen S, van Veen J J, Makris M. Coagulation Department, Royal
Hallamshire Hospital, Sheffield.
P26
THE ‘USEFULNESS’ OF A VERY HIGH D-DIMER
Rachel Heath, Sarah Horton, Vanessa Martlew, Jecko Thachil
Department of Haematology, Royal Liverpool University Hospital, Liverpool, UK. L7 8XP.
10
P27
PKC AND AKT-DEPENDENT INHIBITION OF GSK3 MODULATES PLATELET FUNCTION
Samantha F. Moore, Marion T.J. van den Bosch, Roger W. Hunter and Ingeborg Hers
School of Physiology and Pharmacology, University of Bristol, Bristol, BS8 1TD, UK.
P28
DIRECT INTERACTION WITH CANCER CELLS IS NECESSARY TO INDUCE PLATELET
ACTIVATION
Annachiara Mitrugno, Niamh Moran.Royal College of Surgeons In Ireland, 123 St Stephen Green,
Dublin 2
P29
GAUCHER DISEASE: AN INHERITED DISORDER WITH AN ACQUIRED BLEEDING DIATHESIS
AS Thomas1,2, DA Hughes1,2, AB Mehta2 & K Gomez1,3
1Department of Academic Haematology, University College London
2Lysosomal Storage Disorders Unit & 3Katherine Dormandy Haemophilia Centre & Thrombosis Unit,
Royal Free Hospital, London NW3 2QG
P30
EVALUATION OF A NEW ADAMTS-13 ANTIBODY ASSAY.
Fretwell RE, Van Veen J, Kitchen S. Sheffield Haemophilia and Thrombosis Centre, Royal
Hallamshire Hospital, Glossop Road, Sheffield, S10 2JF.
P31
BAMBI (BMP AND ACTIVIN MEMBRANE BOUND INHIBITOR) - A NOVEL REGULATOR OF
PLATELET PRODUCTION AND FUNCTION
Isabelle I. Salles, David A. Lane James T.B. Crawley. Centre for Haematology, Imperial College
London, 5th Floor Commonwealth Building, Hammersmith Hospital Campus, Du Cane Road, London,
W12 0NN
P32
DEFINING THE ROLE OF RHOF (RIF) IN PLATELETS
R. Goggs1, J.S. Savage1, H. Mellor2, A.W. Poole1 1School of Physiology and Pharmacology, 2School
of Biochemistry, Faculty of Medical and Veterinary Sciences, University of Bristol, University Walk,
Bristol BS8 1TD
P33
ACTIN ANTAGONISTS DECREASE GPVI DIMERIZATION AND MODIFY THE DISTRIBUTION OF
GPVI IN PLATELET CYTOSKELETAL FRACTIONS
Stephanie M. Jung,1 Steve P. Watson,2 Richard W. Farndale,1 and Masaaki Moroi1, 1Department of
Biochemistry, University of Cambridge, Cambridge, UK and 2 Centre for Cardiovascular Sciences,
University of Birmingham, UK
P34
A COMPARATIVE EVALUATION OF A NEW VON WILLEBRAND FACTOR ACTIVITY ASSAY
WITH AN ESTABLISHED AUTOMATED PLATELET BASED RISTOCETIN COFACTOR ASSAY
AS Lawrie1, F Stufano 2, MT Canciani2, IJ Mackie1, SJ Machin1, F Peyvandi1, 2. 1Haemostasis
Research Unit, Department of Haematology, University College London, London, UK. 2U.O.S.
Dipartimentale per la Diagnosi e la Terapia delle Coagulopatie , A. Bianchi Bonomi Hemophilia and
Thrombosis Center, Fondazione I.R.C.C.S. Ca' Granda, Ospedale Maggiore Policlinico, Università
degli Studi di Milano and Luigi Villa Foundation, Milan, Italy
11
P35
PLATELET FUNCTION AND ACUTE WHOLE BLOOD PROSTANOID FORMATION IN TWO
PATIENTS POSSESSING A HOMOZYGOUS MUTATION IN THE GENE ENCODING CYTOSOLIC
PHOSPHOLIPASE A2α
Kirkby NS1,2, Brooke MA3, Longhurst HJ4, Plagnol V5, Kelsell DP3, MacDonald TT3 , Mitchell JA2,
Warner TD1. 1William Harvey Research Institute, Barts & the London School of Medicine, Queen Mary
University of London, UK; 2National Heart & Lung Institute, Imperial College, London, UK; 3Blizard
Institute, Barts & the London School of Medicine, Queen Mary University of London, UK; 4Department
of Clinical Immunology, Barts Health NHS Trust, London, UK; 5Darwin Building, University College
London Genetics Institute, University College London, UK;
P36
IDENTIFICATION OF A NOVEL THROMBOXANE A2 RECEPTOR N42S VARIANT THAT IS NOT
GLYCOSYLATED RESULTING IN REDUCED SURFACE EXPRESSION
S. Nisar1, B.B. Dawood2, M.L.Jones3, S.Murden3, A.D. Mumford3, J.T Wilde4, S.P.Watson2 & S.J.
Mundell1 on behalf of the UK GAPP Study Group. 1School of Physiology and Pharmacology,
University of Bristol, Bristol, BS8 1TD; 2Centre for Cardiovascular Sciences, College of Medical and
Dental Sciences, University of Birmingham, Birmingham, B15 2TT; 3Bristol Heart Institute & School of
Cellular and Molecular Medicine, University of Bristol, Bristol, BS2 8HW; 4West Midlands Adult
Haemophilia Centre, University Hospital Birmingham National Health Service (NHS) Trust,
Birmingham, United Kingdom.
P37
FIBRINOGEN -CHAIN CROSS-LINKING INCREASES FIBRE THICKNESS OVER TIME, AND
STRAIGHTENS FIBRIN FIBRES
Cédric Duval1, Victoria Ridger2, Helen Philippou1, Robert Ariëns1.
1Mechanisms of Thrombosis; Division of Cardiovascular and Diabetes Research; The Leeds Institute
of Genetics, Health and Therapeutics; University of Leeds; UK.
2Department of Cardiovascular Science; Faculty of Medicine, Dentistry and Health; University of
Sheffield; UK.
P38
A SYSTEMS-BIOLOGY STUDY OF THE COAGULATION CASCADE
Joanne Dunster1,2, Helen Byrne3, Susan Franks4, Jon Gibbins2 , John King5. 1 Institute for
Cardiovascular and Metabolic Research, School of Biological Sciences, 2 Department of Mathematics
and Statistics, University of Reading , 3 Oxford Centre for Collaborative Applied Mathematics,
Mathematical Institute, Oxford OX1 3LB and Department of Comp. Sci., Parks Road, Oxford OX1
3QD,4 Health and Safety Laboratory, Harpur Hill, Buxton, Derbyshire SK17 9JN , & 5 Centre for
Mathematical Medicine, School of Mathematical Sciences, University of Nottingham, University Park,
Nottingham, NG7 2RD, UK .
P39
A SYSTEMS BIOLOGY STUDY OF PLATELET GPVI SIGNALLING
Joanne Dunster1,2, Francoise Mazet1, Sakthi vel Vaiyapuri1, Chris I. Jones1, Will Kaiser1, Marcus
Tindall1,2, Michael J. Fry1, Jonathan M. Gibbins1. 1Institute for Cardiovascular and Metabolic
Research, School of Biological Sciences, University of Reading, 2Department of Mathematics and
Statistics, University of Reading.
P40
PLATELET PHENOTYPE ASSOCIATED WITH THE LOSS-OF-FUNCTION
Trp29CysTHROMBOXANE RECEPTOR VARIANT
S. Westbury1, S. Nisar2, M.L. Jones1, L. Darnige, C3. Bachelot-Loza, S3. Gandrille3, F. Zinzindoue3, AM. Fischer3, S.J. Mundell2, P. Gaussem3, A.D. Mumford1 and the UK GAPP study group. 1 Bristol
Heart Institute, University of Bristol, Bristol, BS2 8HW; 2 School of Physiology and Pharmacology,
University of Bristol, Bristol, BS8 1TD; 3 Université Paris Descartes, Sorbonne Paris Cité, Paris;
INSERM UMR-S765, Faculté de Pharmacie, Paris and AP-HP, Hôpital Européen Georges Pompidou,
Paris.
12
P41
FXIII POLYMORPHISMS AND DIABETES IN ABDOMINAL AORTIC ANEURSYM
Macrae F, Lee Evans H, Sohrabi S, Johnson A, Scott DJA, Ariëns RAS. Mechanisms of Thrombosis;
Division of Cardiovascular and Diabetes Research; The Leeds Institute of Genetics, Health and
Therapeutics; University of Leeds; UK
P42
RITUXIMAB FOR THE TREATMENT OF THROMBOTIC THROMBOCYTOPENIC PURPURA:
BENEFITS OF EARLY ADMINISTRATION IN ACUTE EPISODES AND ROLE OF ELECTIVE
TREATMENT IN PREVENTING RELAPSE
Westwood J1, Webster H1, McGuckin S2, Machin SJ1, Scully M2. 1Haemostasis Research Unit,
University College London, London, United Kingdom. 2University College London Hospital, London,
United Kingdom
P43
PILOT GENOME-WIDE ASSOCIATION STUDY OF THROMBOTIC THROMBOCYTOPENIC
PURPURA: ASSOCIATIONS IN HLA II and III AND B-CELL DEVELOPMENT.
Heelas EO, 1 Eu-Ahsunthornwattang J,2 Petridis C,4 Papouli E,4 McDonald V,1 Machin S,1 Cordell H,2
Scully, M.1,3 . 1 Haemostasis Research Unit, Department of Haematology, University College London,
London, WC1E 6HX. 2 Institute of Genetic Medicine, Newcastle University, International Centre for
Life, Newcastle, NE1 3BZ. 3 University College London Hospital, Euston Road, London, NW1 2BU. 4
Biomedical Research Centre, King’s College London, St Thomas’ Street, London, SE1 9RT
P44
THE ROLE OF FAK FAMILY MEMBERS IN GPVI-DEPENDENT REACTIVE OXYGEN SPECIES
FORMATION
Naadiya Carrim§, Tony G. Walsh§, Alessandra Consonni¥, Mauro Torti¥, Michael C. Berndt§¤, Pat
Metharom§. § Department of Experimental Medicine, Royal College of Surgeons in Ireland, Dublin,
Ireland. ¤ Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland. ¥ Department of
Biochemistry, University of Pavia, Italy
P45
INVERSION OF CHROMOSOME 5 AS A NEW MECHANISM OF HERMANSKY-PUDLAK
SYNDROME 2.
Matthew Jones1, Sherina Murden1, Viv Maloney2, Richard Manning3, Kimberly Gilmour4, V
Bharadwaj5, J de la Fuente5, Subrana Chakravorty5, and Andrew Mumford1. 1 Bristol Heart Institute &
School of Cellular and Molecular Medicine, University of Bristol.2 Wessex Regional Genetics
Laboratory, Salisbury District Hospital, Salisbury. 3 Department of Haematology, Imperial College
Academic Health Care Trust, Hammersmith Hospital, London. 4 Department of Immunology, Great
Ormond Street Hospital for Children NHS Trust, London 5 Department of Paediatric Haematology, St
Marys Hospital, Imperial College Healthcare NHS Trust.
P46
ABNORMALITIES IN PLATELET COUNT AND COAGULATION SCREEN IN INTENSIVE CARE
Y. Arunan, Critical Care Unit, Royal Liverpool University Hospital. C. L. Khoo, Critical Care Unit, Royal
Liverpool University Hospital. J. Walker, Critical Care Unit, Royal Liverpool University Hospital. J.
Thachil, Department of Haematology, Royal Liverpool University Hospital.
P47
PLATELET COUNTING ON THE ACCURI C6 FLOW CYTOMETER.
Andrew Masters and Paul Harrison. Oxford Haemophilia & Thrombosis Centre, Churchill Hospital and
Department of Haematology, The John Radcliffe, Oxford University Hospitals NHS Trust, Oxford, UK.
13
P48
P-SELECTIN AS A SIMPLE-TO-USE APPROACH FOR ASSESSING PLATELET FUNCTION IN
CARDIAC PATIENTS: A COMPARISON WITH FOUR OTHER PLATELET FUNCTION TESTS
Sue Fox, Jane May, Andrew Johnson, Ann White, Jackie Glenn, Natasha Dovlatova, Yanushi
Wijeyeratne, Stan Heptinstall. Cardiovascular Medicine, University of Nottingham, Queens Medical
Centre, Nottingham, UK
P49
EVALUATION OF THE OPTIMUL ASSAY AS A TOOL TO DETECT EX VIVO PLATELET
INHIBITION IN ACUTE CORONARY SYNDROME PATIENTS RECEIVING DUAL ANTIPLATELET
THERAPY
Jane May1, Natalia Dovlatova1, Nicholas S. Kirkby2, Timothy D. Warner2 and Sue Fox1
1Cardiovascular Medicine, University of Nottingham, Queens Medical Centre, Nottingham UK and
2The William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,
Queen Mary University of London, Charterhouse Square, London, UK
P50
WHOLE BLOOD PLATELET AGGREGATION MEASURED BY SINGLE PLATELET COUNTING
USING A NEW DOUBLE FIXATION APPROACH TO FACILITATE REMOTE TESTING
Mohammad Algahtani, Jane May, Ann White, Natalia Dovlatova, Andrew Johnson, Stan Heptinstall,
Sue Fox.
Cardiovascular Medicine, University of Nottingham, Queen’s Medical Centre, Nottingham UK
P51
F7 MUTATION ANALYSIS AND THE INFLUENCE OF POLYMORPHISMS IN THE SCOTTISH FVII
DEFICIENCY COHORT.
Gillian McGaffin1, Anne White1, R Campbell Tait2, Catherine Bagot2, Henry Watson3 and David
Stirling1. 1 Haematology Department, Edinburgh Royal Infirmary, 51 Little France Crescent,
Edinburgh, EH16 4SA, 2 West of Scotland Haemophilia Centre, Glasgow Royal Infirmary, 84 Castle
Street, Glasgow, G4 0SF. 3 Haematology Department, Aberdeen Royal Infirmary, Foresterhill,
Aberdeen, AB25 2ZN.
P52
SIGNALLING VIA CLEC-2 GENERATES A PROCOAGULANT RESPONSE IN PLATELETS
Mohammed A Alsahli1, Jacky A Appleby1, Hassan S Hamali1, Joy R Wright1, Steve P Watson2, Alison
H Goodall1. 1Department of Cardiovascular Sciences, University of Leicester, and 2Centre for
Cardiovascular Sciences Institute of Biomedical Research, University of Birmingham.
P53
STIM1 BINDS TO A NUMBER OF PROTEINS IN HUMAN PLATELETS.
Archana Ambily and Kalwant S. Authi. Cardiovascular Division, Kings College London, 150 Stamford
Street. London SE1 9NH
P54
COMPARISON OF THE IN VITRO ACTIVITY OF PLASMA-DERIVED AND RECOMBINANT FIX
CONCENTRATES. Yao Yu, Mike A Laffan, Carolyn Millar. Centre for Haematology, Imperial College
London, London UK.
P55
HOW COMMON IS OSTEOPOROSIS WITH LOW MOLECULAR WEIGHT HEPARIN?
Klaire Exarchou, Jecko Thachil. Department of Haematology, Royal Liverpool University Hospital,
Liverpool, UK. L7 8XP.
14
P56
VON WILLEBRAND FACTOR SIALYLATION STATUS MODIFIES ITS FUNCTION UNDER SHEAR
STRESS
Agata A Nowak, Mike A Laffan, Thomas AJ McKinnon. Department of Haematology, Faculty of
Medicine, Hammersmith Hospital Campus, Imperial Collage London,
P57
AN IN VITRO FLOW ASSAY TO ANALYSE VON WILLEBRAND FACTOR PROTEOLYSIS BY
ADAMTS13
Thomas A J McKinnon, Agata A Nowak, Aia Mehdi, Carol Wooding and Mike A Laffan. Department of
Haematology, Faculty of Medicine, Hammersmith Hospital Campus, Imperial College London.
P58
THROMBUS FORMATION UNDER FLOW CONDITIONS IS ENHANCED AFTER DESMOPRESSIN
TREATMENT IN PATIENTS WITH POSTOPERATIVE BLEEDING COMPLICATIONS
Frauke Swieringa1, Geert-Jan Kuiper1, Marcus D. Lancé2, Johan W.M. Heemskerk1 and Paola E.J
van der Meijden1 Department of Biochemistry, Cardiovascular Research Institute Maastricht,
Maastricht University, Maastricht, The Netherlands. 2 Department of Anesthesiology, Maastricht
University Medical Centre, Maastricht, The Netherlands
P59
PLATELET FUNCTION TESTING – RATIONALISING RECOMMENDED AGONISTS AND
CONCURRENT USE OF A REMOTE WHOLE BLOOD ASSAY
Gillian C. Lowe1*, Marie Lordkipanidze1*, Natalia Dovlatova1,2, Ban Dawood1, Stan Heptinstall2, Sue
Fox2 and Steve Watson1 on behalf of the UK GAPP Study Group. *These authors contributed equally
to this work. 1Centre for Cardiovascular Sciences, Institute of Biomedical Research, College of
Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, 2Cardiovascular
Medicine, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH
P60
ROLE OF GAP JUNCTIONS AND CONNEXIN HEMICHANNELS IN PLATELET FUNCTION
Sakthivel Vaiyapuri1, Chris I. Jones1, Parvathy Sasikumar1, Leonardo A. Moraes1, Marfoua S. Ali1,
Tanya Sage1, Alexander M. Simon2, Martyn P. Mahaut-Smith3, Jonathan M. Gibbins1.
1Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of
Reading, Reading, United Kingdom. 2Department of Physiology, University of Arizona, Tucson, USA.
3Department of Cell Physiology & Pharmacology, University of Leicester, Leicester, United Kingdom
P61
PURIFICATION AND FUNCTIONAL CHARACTERISATION OF RHINOCETIN, A SNACLEC FROM
THE VENOM OF BITIS GABONICA RHINOCEROS
Sakthivel Vaiyapuri1, Marfoua S. Ali1, Robert A. Harrison2, Andrew B. Bicknell1, Jonathan M. Gibbins1.
1Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of
Reading, Reading, United Kingdom 2Liverpool School of Tropical Medicine, Liverpool, United
Kingdom