695
Table S1
Intact soil physicochemical properties and enzyme activity
Tree species
Dacrydium gracilis
(conifer)
Lithocarpus clementianus
(broadleaf)
(%)
79.0 (0.36)
3.90 (0.05)*
3.08 (0.06)
79.3 (1.22)
4.08 (0.05)
3.13 (0.05)
(μg g-1)
(μg g-1)
3.65 (0.39)
0.37 (0.08) †
4.52 (0.75)
0.20 (0.04)
2309 (240)
0.462 (0.038)**
1.552 (0.123)
1799 (336)
0.313 (0.023)
1.481 (0.101)
(μmol g-1 h-1)
(μmol g-1 h-1)
16.6 (0.81)
0.174 (0.025)
12.5 (2.18)
0.227 (0.057)
(μmol g-1 h-1)
0.193 (0.012)**
0.750 (0.135)
0.625 (0.202)
0.276 (0.016)
1.402 (0.316)
N.D.
5.85 (0.55)**
0.04 (0.08)
9.48 (0.79)
0.29 (0.21)
0.125 (0.009) †
0.171 (0.021)
Soil phycicochemical properties
Soil Water Content
pH (H2O)
pH (KCl)
Total phenol1
Condensed tannins1
Soil microbial community
Total lipid abundance2
Fungi/Bacteria ratio2
Gm+/Gm– ratio2
Soil enzyme activity
Acid phosphatase
-D-glucosidase
N-Acetylglucosamidase
Phenol oxidase
Peroxidase
(nmol g-1)
(μmol g h )
(μmol g-1 h-1)
-1
-1
Soil C and N flux
N mineralization rate
Nitrification rate
(μg g-1 d-1)
(μg g-1 d-1)
Soil respiration rate
(mg CO2 g-1 h-1)
696
1
697
Ushio et al. 2008. Values of the soil enzyme activities and carbon and nitrogen flux were measured for
698
soil samples used for the laboratory incubation experiment before the incubation. Parentheses indicate
699
standard error of mean. Significant differences between the two species were indicated by †P < 0.1, *P
700
< 0.05 and **P < 0.01 (t-test).
data of the surface organic soil beneath each tree crown, from Ushio et al. 2010. 2data modified from
48
701
Table S2
F-values by linear mixed models in the tannin addition experiment under field condition
Effects
Tree
Tannin
Degree of freedom
1
1
Tree × Tannin
1
Total lipid
0.734
2.904
0.707
Fungi/Bacteria ratio
14.78**
0.268
0.113
33.36***
0.015
2.497
0.102
0.013
1.771
1.511
4.420†
0.656
0.585
0.238
1.662
Acid phosphatase
3.280
8.194*
0.347
-D-glucosidase
N-acetylglucosamidase
Phenol oxidase
0.003
6.389*
1.369
9.485*
3.721†
4.403†
0.062
0.653
4.497†
Peroxidase
2.358
1.325
0.004
Soil respiration rate§
0.902
13.51*
0.715
7.307*
2.101
3.254
7.375*
2.909
Soil microbial property
Gm+/Gm− ratio
Lipid profile PC1 (40.6%)
Lipid profile PC2 (18.2%)
Lipid profile PC3 (10.5%)
Soil mineralization activity
Soil physical property
pH (H2O)
pH (KCl)
0.069
702
Values indicate F-statistics. † P < 0.10, * P < 0.05, ** P < 0.01, *** P < 0.001. §Additive mixed model
703
was applied only for the soil respiration rate because the respiration rate was monitored periodically.
49
704
Table S3
705
condition
F-values by additive mixed models in the tannin addition experiment under laboratory
Soil properties
Tree
Tannin
Tree × Tannin
Days
Degree of freedom
1
1
1
§
Total lipid
1.694
0.143
Fungi/Bacteria ratio
20.20***
0.503
8.864**
3.902†
2.448
14.92****
10.15***
13.56***
2.527
8.062**
1.951
0.747
2.610
0.595
0.431
1.073
1.811
1.505
0.127
2.599
0.762
0.002
0.076
75.18****
28.0****
11.75***
1.695
0.131
0.796
0.447
61.81****
10.76**
0.068
Soil microbial property
Gm+/Gm− ratio
Lipid profile PC1 (39.8%)
Lipid profile PC2 (21.8%)
Lipid profile PC3 (9.5%)
59.12***
1.519
2.174
4.672*
12.76****
Soil mineralization activity
Acid phosphatase
-D-glucosidase
N-acetylglucosamidase
Phenol oxidase
Peroxidase
Soil respiration rate
Cumulative CO2
4.140*
13.28***
10.78**
5.198*
0.469
0.105
4.380*
0.015
3.536†
3.221†
38.25****
2169****
7.098**
13.62***
4.581*
3.052†
10.02****
383.3****
Soil physical property
pH (H2O)
pH (KCl)
3.801†
0.429
706
Values indicate F-statistics. † P < 0.10, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
707
§Degree of freedom for days is not shown because additive mixed modeling does not calculate a degree
708
of freedom for a term for which the smoothing function applied.
50