Department of Microbiology
Quality Manual
Section: Process Control
Prepared by: QA Committee
Issued by: Laboratory Manager
Approved by: Laboratory Director
Policy QPCMI10001.09
Page 1 of 4
Subject Title: Analytical Process –
Bacteriology
Reagent/Materials/Medi
a List
Original Date: December 14, 2004
Revision Date: March 13, 2007
Annual Review Date: May 31, 2013
Acidine Orange - Difco
Acid-fast stain – VWR / Fisher
ALA discs – Fisher
Amies transport medium – Starplex/Quelab
Antibiotic Disks - Oxoid
Arabinose Diagnostic Tablets - ProLab Diagnostics
Autostreaker (Isoplater)
Bacteroides Bile Esculin (BBE) Agar – OXOID
Beta-glucoronidase (MUG)
Bile Esculin Agar (BE) - OXOID
Blood Agar (BA) – Oxoid
Blood Egg Albumin Agar (BEAA) – Biomedia
Blood with Chloramphenicol and Gentamicin Agar (BCGM)
Brain Heart Infusion Agar with 6 mg/L vancomycin - Oxoid
Brain Heart Infusion Agar (plain) - Oxoid
Brain Heart Infusion Broth (3 mL and 10 mL) - Oxoid
Calcofluor white – Difco
Campylobacter plate (Campy) – OXOID
Cary-Blair transport medium – Central Stores
Catalase (3% H2O2) – Sigma
Cefsulodin, Irgasan, Novobiocin agar (CIN) – PML
Cefinase discs – VWR
Centrifuge (At least 3500 rpm)
Centrifuge tubes (50 mL)
Cephalothin 30g disc – Oxoid
Chocolate Agar (CHOC) – Oxoid
Colistin – Nalidixic Acid Agar (CNA) – Oxoid
Denim Blue Chromogenic Agar for MRSA - Oxoid
E. coli 0157 Latex Agglutination Test – Oxoid
Enterococcus Agar with Vancomycin (EV)
Eosinophil stain – Hansel
Esculin Base Medium (EBM)
UNIVERSITY HEALTH NETWORK/MOUNT SINAI HOSPITAL, DEPARTMENT OF MICROBIOLOGY
NOTE: This is a CONTROLLED document. Any documents appearing in paper form that are not stamped in red "MASTER COPY"
are not controlled and should be checked against the document (titled as above) on the server prior to use.
D:\533570753.doc
Department of Microbiology
Quality Manual
Section: Process Control
Policy QPCMI10001.09
Page 2 of 4
Subject Title: Analytical Process –
Bacteriology
Reagent/Materials/Media
List
Fastidious Anaerobic Agar (BRUC) - Oxoid
Fastidious Anaerobic Broth (THIO) – Oxoid
Fungifluor – Polysciences
GenProbe for S. aureus
Glass slides
Gonogen - Medicorp
Gram Stain – Quelab
Group B Strep Broth (Todd-Hewitt with Gent and NA) - Oxoid
Haemophilus Isolation Agar (HI) - Oxoid
Hektoen (HEK) – OXIOD
Inhibitory Mould Agar (IMA) – Oxoid
Isolator Tubes – Oxoid
Kanamycin/Vancomycin Agar (KV) - Oxoid
Kovac’s Reagent– Refer to Media Manual for preparation
LAP Disks - Remel
0.01 mL sterile calibrated loop
0.001 mL sterile calibrated loop
MacConkey Agar (MAC) – Oxoid
MacConkey Sorbitol (SMAC) - OXOID
MacConkey Agar with 2 mg/L cefpodoxime - Oxoid
Martin-Lewis (ML) - Biomedia
Mueller Hinton Agar (small and large) - Oxoid
Mueller-Hinton Agar powder– Becton Dickinson
Martin-Lewis Agar (ML) – Quelab
MRSA Screen - Denka Seiken
1% methyl- -D-glucopyranoside (MGP) Broth -Oxoid
Manitol Salt Agar powder– Difco Laboratories
mEnterococcus Agar powder – Difco Laboratories
Nalidixic Acid 30 g disc – OXOID
Novobiocin disc (NB)
NaCl powder – Sigma Chemical Company
UNIVERSITY HEALTH NETWORK/MOUNT SINAI HOSPITAL, DEPARTMENT OF MICROBIOLOGY
NOTE: This is a CONTROLLED document. Any documents appearing in paper form that are not stamped in red "MASTER COPY"
are not controlled and should be checked against the document (titled as above) on the server prior to use.
D:\533570753.doc
Department of Microbiology
Quality Manual
Section: Process Control
Policy QPCMI10001.09
Page 3 of 4
Subject Title: Analytical Process –
Bacteriology
Reagent/Materials/Media
List
Oxacillin powder– Sigma Chemical Company
OFbase, Colistin, Bacitracin & Lactose Agar (OCBL) - Oxoid
ONPG-PAM – OXOID
Oxidase Strip - API
Oxidase reagent
PBP2a agglutination kit – Denka Seiken
Dehydrated Rabbit plasma – Remel
PYR Disks - Remel
PYR Dry Slide - Oxoid
Rapid Yeast plus Strips - Oxoid
Rapid Ana II Strips – Oxoid
Salmonella antisera – Abbott Diagnostic Inc.
Salmonella O (A-S)
Salmonella H (phase 1 and 2)
Salmonella H (phase 2)
Salmonella Vi
Salmonella 2-O
Salmonella 4-O
Salmonella 3,10,15,19-O
Salmonella 6,7-O
Salmonella 8-O
Salmonella 9-O
Selenite Broth (SEL) – Med Prep
Shigella antisera –Abbott Diagnostics Inc.
S. sonnei (phase 1 and 2)
S. flexneri polyvalent
S. dysenteriae
S. boydii polyvalent 1
S. boydii polyvalent 2
S. boydii polyvalent 3
Sodium Pyruvate (PYRA) – Oxoid
Spot Indole reagent
Streptococcus Latex agglutination – Pro Lab Diagnostics
Staphylococcus Latex agglutination (Pastorex Staph Plus) – bioRad
Staphylococcus aureus Accuprobe - Gen-Probe
UNIVERSITY HEALTH NETWORK/MOUNT SINAI HOSPITAL, DEPARTMENT OF MICROBIOLOGY
NOTE: This is a CONTROLLED document. Any documents appearing in paper form that are not stamped in red "MASTER COPY"
are not controlled and should be checked against the document (titled as above) on the server prior to use.
D:\533570753.doc
Department of Microbiology
Quality Manual
Section: Process Control
Policy QPCMI10001.09
Page 4 of 4
Subject Title: Analytical Process –
Bacteriology
Reagent/Materials/Media
List
Toluidine blue-O DNA plate (Q-lab)
Trypticase Soy Broth (TSB) – OXOID
Triple Sugar Iron (TSI) – OXOID
Triphenyl-tetrazolium chloride (TTC)
Tube coagulase - Medox
Urea Broth – Oxoid
Vacutainer tubes
Vancomycin powder– Sigma Chemical Company
Vancomycin Screen plate
Vitek susceptibility cards – BioMerieux
Vitek identification cards – BioMerieux
Vortex mixer
Xylose Diagnostic Tablets - ProLab Diagnostics
UNIVERSITY HEALTH NETWORK/MOUNT SINAI HOSPITAL, DEPARTMENT OF MICROBIOLOGY
NOTE: This is a CONTROLLED document. Any documents appearing in paper form that are not stamped in red "MASTER COPY"
are not controlled and should be checked against the document (titled as above) on the server prior to use.
D:\533570753.doc