Thomas J. Waeghe, Ph. D.
Dr. Thomas J. Waeghe received his Bachelor of Science degree in 1977 from Michigan
Technological University, where he was also named “Outstanding Senior Student” for the
Department of Chemistry and Chemical Engineering. He obtained his Ph.D. in Analytical
Chemistry from the University of Colorado in Boulder, Colorado in 1982. His doctoral dissertation dealt with environmental chemistry methodology for trace analysis of aldehydes, and with the scale-down and optimization of glycosyl linkage composition analysis for structural elucidation of microgram quantities of complex carbohydrates.
After obtaining his Ph. D., Dr. Waeghe joined DuPont Agricultural Products in 1983. While at
DuPont he developed a wide variety of GC and HPLC trace-level, impurity, and assay methods to support the product development and improvement, registration, and commercialization of a variety of herbicides, fungicides, and insecticides. He also championed the use of systematic method development for RPLC using DryLab
® computer simulation software, and was a leader in the development and preparation of a new method manual, which contained improved processes for managing change and written guidance and recommended practices for the development, optimization, validation and management of analytical methods.
From 1999 to 2008 Tom worked with Chromatography, Inc. and Agilent Technologies, where he provided expert phone and email technical support for all of Agilent’s LC, GC and instrument consumables for North America, including ZORBAX HPLC columns. In 2008 Tom joined
MAC-MOD Analytical, which was the original exclusive distributor for ZORBAX columns for then-Rockland Technologies in the United States from 1986-1997. Since joining MAC-MOD
Analytical last July, he has provided phone and email technical support and has contributed to technical literature and applications for HALO
®
and ACE
®
HPLC columns.
On a personal note, Tom has been a St. Louis Cardinals fan since 1966, and his all-time favorite baseball player is Bob Gibson. One of the items on his bucket list is to attend a game in St.
Louis, and to see the Cardinals win.

Abstract
HALO
®
Fused-Core
®
Columns for High Throughput and High Resolution Separations:
Importance of Extracolumn Volume and Practical Applications
With the introduction of columns packed with sub-two-micron particles for high-throughput and high-efficiency separations over five years ago, a number of HPLC practitioners have used them for improving productivity or for separating very challenging samples in their laboratories.
Moreover, the significantly higher pressures necessary to fully utilize such columns often require the purchase of instrumentation capable of delivering pressures of 600-1000 bar. As a result, many researchers and workers have tended to avoid purchasing new high pressure instruments for their laboratories, because of the increased expenditures and/or the lack of familiarity with the requirements and limitations of the new technologies. Fortunately, the innovative development and commercialization of columns packed with superficially porous, 2.7-micron
Fused-Core
®
particles have now opened the arena of fast UHPLC and ultrahigh resolution separations to those laboratories with conventional 400-bar instruments.
In this presentation we will discuss some of the theory behind and recent findings associated with Fused-Core
®
particles such as HALO
® . We’ll review extracolumn band broadening and the impact it has on the performance of high-efficiency, low-volume columns. We’ll also describe which modifications to make to a typical 400-bar HPLC system to obtain the “UHPLC-like” performance at pressures that are 40-50% of those obtained for sub-two-micron columns. Lastly,
® some example applications will be presented of isocratic and gradient separations using HALO
Fused-Core
®
columns.