TITLE: A Pine Cone Mystery QUESTION: How does water affect pine cone scales? RESEARCH: The scales on a pine cone close inward when they are exposed to water. When the cells at the base of each scale are saturated with water, the resulting pressure causes the scales of the pine cone to close. This process keeps the seeds from leaving the pine cone when it is wet. If the seeds were to fall from the pine cone when they were wet, then they would drop close to the mother tree and have to compete with it for water and nutrients from the soil. The seeds do much better when the pine cone is open on dry, windy days. The wind blows through the pine cone and carries the seeds to a more ideal location to grow. Sources: "Gone With the Wind Pine Seed Dispersal." AgEd. N.p.. Web. 11 Nov 2012. <http://aged.uga.edu/browseable_folders/ClassStartersMentalSetFadctsFiguresIdeas /Gone_With_The_Wind_Pine_Seed_Dispersal.doc>. Durham, Tim Dr.. "Pine Cone Opening Mechanism." Newton Ask A Scientist. Argone National Lab U.S. Dept. of Energy, 01 Jun 2012. Web. 11 Nov 2012. <http://www.newton.dep.anl.gov/askasci/bot00/bot00947.htm>. Reeves, Walter. "Pine Cones - Why Do They Close Up?." Walter Reeves.Com. The Simple Gardener, Inc., Web. 11 Nov 2012. <http://www.walterreeves.com/gardening-q-anda/pine-cones-why-do-they-close-up/>. VARIABLES: Independent Variable: Water Dependent Variable: Appearance of the pine cones scales Control Variables: Same type of pine cones Same size pine cones Same type of containers Same location of containers Same amount of time pine cones are in the container. HYPOTHESIS: If I place one pine cone in water and one pine cone in air, then the pine cone in water will close its scales, because the cells at the bottom of each scale become saturated causing water pressure to build up and force the scales to move inward (close), thus protecting the seeds located inside the scales. MATERIALS: (2) Pine cones (from the same pine tree) that are the same size and with open scales (2) 1,000 ml containers 800 ml of tap water 1,000 ml plastic beaker PROCEDURE 1) Obtain two pine cones that are the same size and have open scales and are from the same pine tree. 2) Place the two 1,000 ml containers side-by-side on a flat surface, 3) Fill one of the containers with 800 ml of water using the 1,000 ml plastic beaker. 4) Simultaneously (both at the same time), put one of the pine cones in the 1,000 ml container filled with water and put the other pine cone in the empty 1,000 ml container. 5) Repeat steps 1 – 4 two more times so that there are three trials of the experiment. 6) Record observations for all three trials at 9:00 a.m. the next day. RESULTS: THE BELOW PHOTOGRAPHS WERE TAKEN AT 9:00 A.M. THE DAY AFTER THE EXPERIMENT WAS SET UP. Trial #1 Water Trial #2 Water Trial # 3 Water All of the scales on the pine cones in the water closed. The water turned a pale yellow color. The pine cones floated at the surface of the water. Trial #1 Air Trial #2 Air All of the scales on the pine cones in the air stayed open. Trial #3 Air CONCLUSION: 1) Tell whether your hypothesis is correct or incorrect, and support your decision by using specific examples from the data collected. My hypothesis was correct. The results from all three trials showed that the scales on the pine cones closed when place in water. 2) Describe any difficulties with the experiment? It was difficult to find pine cones that were all the same size and from the same tree. 3) Explain any reason to disbelieve your results? I was unable to monitor the pine cones the entire length of the experiment. It is possible that another variable could have been introduced to the experiment. For example, someone could have poked at the pine cones when I was not watching. 4) Write about any research you’ve done to clarify your results. My results showed that the pine cones floated in water. I remembered reading about why things float in class, so I went to my Weather and Water Resources book, and it stated on page 30 that “materials less dense than water float in water.” That means the pine cone is less dense than the water. Source: Weather and Water Reources. Nashua : Delta Education, 2004. 30. Print. 5) Do the results of your experiment cause you to have any further questions that could be answered through conducting another experiment? Water is 100% humidity. Will the pine cone close at a lesser percent of humidity?