3/21/11 Rabbit Surgeries See procedure notes attached beneath these records.
Todd Kitten, x6-7010, cell: 301-4948, IACUC # AM10030
Presurgical examination normal? Yes  No  Date______________ Initials ______
Notes:_____________________________________________________________________________
Rabbit Mass (kg)
563 Drug Amounts
aceprom. (10 mg/ml)
ket #
ket + xyl + bupren.
bup #
Card#
(ml) Time/Initials
0.6
pedal reflex
test
15 min
30 min
(87 + 8.7 + 0.013 mg/ml)
glycopyrrolate (0.2 mg/ml)
45 min
Surgery start
Surgery finish
Surgical Notes:
Parameter
Anesthesia
Respiration
Cardiovascular
Cath. placement
Blood loss
Hemostasis
Other
Normal/Good Abnormal Comments
Initials:
Immediate Post-operative observations (behaviors observed, time, initials)
Chewing
Lifting head
Eating paper
Repositioned body
Sitting upright
Other:
Return to cage: Time________PM Initials_______
Buprenorphine injection (0.3mg/ml): Vial #______ Vol. (ml)_______ Time______PM Initials______
Follow-up postoperative daily observations: (1= excellent; 5 = poor. Any problems or other observations
are recorded below in "Other comments." *NC = cages not cleaned before surgery; JC = cages just cleaned)
Item
Date_________
Time
AM
Date_________
Time
PM
Date_________
Time
AM
Date_________
Time
PM
Responsiveness (1-5)
Posture/Activity (1-5)
Respiration normal? (Y/N)
Eating/drinking (Y/N)
Urine (1-5; NC; JC)*
Feces (1-5; NC; JC)*
Buprevol. (ml)
norphine
Vial #
Initials
Other comments_____________________________________________________________
_________________________________________________________________________
_________________________________________________________________________
Inoculation and necropsy notes on back.
Rabbit
_____
Inoculation Notes Date____________ 0.8 ml acepromazine SC Time_______ Initials ______
Strain Inoculated (iv) ______________
Time_______ Initials ______
Incision ____OK
_____Clips removed? Other ___________________________________
Initials ______
Necropsy Notes Date____________ 1.2 ml acepromazine SC Time_______ Initials ______
Euthasol (iv, ear vein) _____ ml Time_______ Initials ______
Catheter location: __________________________________
Other necropsy notes:
Description of Procedures Performed
Date____________
Signed_________________________
For catheterization, rabbits were anesthetized as follows. All rabbits were given 0.6 ml acepromazine SC
(10 mg/ml; on the back). At least 10 minutes later, 0.575 ml/kg xylazine + ketamine + buprenorphine
solution was injected SC at an adjacent site. (Premix is 1 ml of 100 mg/ml xylazine and 0.5 ml of
buprenorphine (0.3 mg/ml) added to a 10 ml bottle of 100 mg/ml ketamine. Final solution is 87 mg/ml
ketamine, 8.7 mg/ml xylazine and 0.013 mg/ml buprenorphine.) This was followed by immediate SC
injection of 0.1 mg (0.5 ml) per kg glycopyrrolate. See previous pages for exact times and amounts of
anesthetic injected. Ten minutes later, the rabbits had their neck, sternum, and ears shaved. Ophthalmic
ointment was applied to each eye. This was followed by swabbing of the neck with betadyne and injections
of 0.5 ml (2.5 mg) bupivicaine SC at the site where the incision was to be made.
Rabbits were then carried to the sterile field and covered with a sterile drape. An incision was made in the
neck above the sternum. The right carotid artery was identified and gently dissected away from
surrounding tissue. The artery was tied off with suture at the head end, and clamped with a vascular clamp
at the base of the neck. An 18G needle was bent and introduced into the artery. This served as a guide for
introduction of a catheter into the artery. The needle and clamp were then removed, and the catheter
threaded through the artery until resistance was met, indicating contact with the aortic valve or insertion
into the left ventricle . The catheter was crimped, tied off with suture, and cut. Sutures were also tied
around the artery just caudal to the point of catheter insertion to secure the catheter and prevent bleeding.
The short remaining head end of the catheter was tucked under the subcutaneous tissue of the neck, and the
incision closed with skin clips. The rabbit was returned to the anesthesia room with a warmed deltathermal
pad for observation until recovered.
All animals were returned to their cages as soon as they were upright and recovered. At the end of the day,
animals received ~0.03 mg/kg buprenorphine SC. Buprenorphine (~0.03 mg/kg) was also supplied every 12
hrs for the next two days. Animals were then monitored every 12 hrs until euthanasia, and provided
buprenorphine if a rabbit showed signs of pain. See previous pages for notes on surgery and recovery.
Two days after catheterization, an inoculum of one or more bacterial strains was injected into the marginal
ear vein, as noted on the previous pages. The inoculum consisted of approximately 107 colony forming
units of bacteria, unless otherwise noted. Rabbits were given 0.8 ml (8 mg) acepromazine subcutaneously
and placed in a restrainer prior to inoculation.
Animals were euthanized the next day. Rabbits were given 12 mg acepromazine subcutaneously and placed
in a restrainer. Two ml euthasol (equivalent to ~780 mg of sodium pentobarbital) was then injected into an
ear vein. If death did not occur immediately, additional euthasol was injected. Thoracotomy immediately
followed euthanasia. All animals were necropsied to determine catheter placement and presence of
vegetations on heart valves. Organisms were recovered by homogenizing these tissues in PBS, then
spreading the homogenates on agar plates and incubating to allow for bacterial growth.
Initials
TK
NZ
XG
CM
Name
Todd Kitten
Nicai Zollar
Xiuchun Ge
Cindy Munro