TITLE: MINIMUM REQUIREMENTS FOR PROTECTIVE ATTIRE IN ANIMAL FACILITIES
Based on NIH Recommendations
Purpose: Provide basis for utilizing a protective program through assessment of risk when
working with various animal species and specific protective measures to reduce the risk, with an
emphasis on personnel protective equipment and hygiene. Principles serving as the basis for
selecting appropriate protective measures include: 1) reducing or avoiding animal-related
accidents by understanding the potential for physical risks unique to each species, i.e. biting,
scratching, kicking, etc; 2) sound personal hygiene based on knowledge of when contamination
is most likely to occur, methods of avoidance and methods of decontamination, as exemplified
by proper use of barrier methods and adequate hand washing practices; and 3) decreasing or
eliminating exposure to zoonotic diseases by understanding common diseases that may be
encountered with each species.
In addition to protection provided by Personal Protective Equipment, PPE, hand washing is an
important adjunct to the use of disposable gloves or in place of exam gloves for prevention of
the spread of infectious organisms or other contaminants to both personnel and animals. While
the use of gloves will greatly decrease the spread of contaminants from a person’s hands, they
do not completely eliminate this transfer due to micro-breaks in the glove materials, regardless
of type (latex, vinyl or nitrile). For effective hand washing, soaps or alcohol-based hand rubs are
recommended.
Definitions:
Uniform: Clothing assigned for use only within the animal facility. It cannot be worn outside the
animal facility without appropriate covering.
Street clothes covering: A garment such as a laboratory coat or coveralls, worn to protect street
clothes from contamination. This garment is not to be worn outside the animal facility. A
unifrom may be substituted for a covering garment.
Disposable exam gloves: Vinyl, latex or nitrile gloves which prevent contamination of skin by
wet or dirty surfaces.
Bite protection gloves: Gloves constructed of materials resistant to punctures, such as Kevlar
and stainless steel mesh, which are worn either over exam gloves or under other protective
gloves to reduce the likelihood of bite punctures.
Arm length bite protection gloves: Heavy, reinforced gloves, usually of leather or similar
material. The sleeves of these gloves should extend up to or over the elbows offering protection
of the hands and forearms. These gloves do not necessarily prevent an animal from biting or
inflicting injury; however, they usually prevent the bite from breaking the skin.
Mucous membrane protection: A device or combination of devices, such as face shields,
surgical face masks combined with protective safety glasses or goggles, etc., which protect the
mouth, nose, and eyes from splash or droplet contamination.
Shoe covering: Stretch booties, usually made of paper or plastic, worn over street shoes to
protect them from contamination. These should not be worn outside the animal facility.
Dedicated footwear may be substituted for shoe coverings. If dedicated shoes are worn, shoe
coverings may be used to cover them when moving outside the facility.
Hand washing: The single most important method in the prevention of the spread of disease to
animals and staff is hand washing. Multiple studies have demonstrated that hand washing
decreases the risk of oral intake of fecal and oral infective agents. Workers should wash their
hands before and after coming into contact with animals. It is not clear that specific antibacterial
hand washing agents are better than ordinary soap and water. It should be emphasized that
even respiratory diseases are most likely transmitted hand to mouth rather than aerosol to lung,
and that hand washing circumvents this route.
Zoonoses: Diseases of non-human animals that may be transmitted to man or may be
transmitted from man to non-human animals.
NONHUMAN PRIMATES (NHP)
Zoonotic Diseases: NHPs and humans are similar enough that many of the same agents that
cause disease in one species cause disease in one species cause disease in the other, and
dissimilar enough that agents relatively asymptomatic in one species can devastate the other.
Cercopithecine herpesvirus-1 (Herpes B), tuberculosis, and various enteric infections are some
of the more common zoonoses associated with NHPs. Even though research NHPs go through
a specific quarantine process, because they may be wild caught and/or living in outdoor housing
areas, they may still harbor certain zoonotic diseases. Table 1 outlines select bacterial, viral and
parasitic agents that are transmissible from NHPs to humans and vice versa, that can be of
concern.
Wounds: All animal procedures should be performed by properly trained personnel, with a
vigilant approach to the prevention of bites, scratches, and splashes both for the minimization of
physical damage and the prevention of disease transmission, especially Cercopithecine
herpesvirus-1 for macaque species. Proper sleeves, mucous membrane protection and
appropriate gloves should be worn when scratches, bites and splashes are possible. The
macaque exposure SOP must be followed when wounds and/or mucous membrane splashes
occur, and all incidents reported.
Protective measures: Table 2 outlines recommendations for specific activities associated with
different risks of exposure. The guidelines assume that NHPs have passed through an
appropriate quarantine.
RODENT and RABBIT SPECIES:
Zoonotic Disease: Zoonotic disease associated with modern rodent and rabbit research holding
facilities in infrequent in incidence due to utilizing specific pathogen free animals and breeding
programs, sentinel health monitoring programs , approved vendor procurements and policies for
the importation of rodents and rodent products. Most zoonotic threats from rodents originate
from wild caught species used for research (which necessitates special PPE requirements and
husbandry), the use of contaminated rodent products or rarely from feral rodents gaining access
to research animals. Human infections have mainly resulted from direct contact with the
animals, their feces or urine, or indirectly by inhaling dried excreta carried on aerosolized dust.
Table 3 lists select agents that are transmissible from rodent and rabbit species to humans that
can be of concern.
Attention to hygiene, to include use of disposable gloves, frequent changing of gloves, careful
hand washing, and use of facemasks will aid prevention of zoonotic disease associated with
rodent and rabbit species.
Allergy: Allergic skin and respiratory reactions are quite common in personnel working with
laboratory animals. Hypersensitivity reactions to animal allergens are serious occupational
health problems that develop in many individuals after repeated exposure. Hypersensitivity
reactions include nasal congestion rhinorrhea (runny nose), sneezing, itching of the eyes,
asthma and a variety of skin manifestations such as redness, localized itching and flaking skin,
and hives. Of the species used in biomedical research, the guinea pig, rabbit, mouse and rat
appear to be the most allergenic. Urinary and salivary proteins from the animal’s fur, bedding,
and caging are known source of allergens.
Methods of prevention include reduction of direct animal contact time, use of biological safety
cabinets, filter tops on animal cages, ventilated caging rack systems, HEPA filtered bedding
dump stations, and protective clothing, masks, or respirators when working with these species.
The N95 dust and mist respirator has been found to be extremely effective in minimizing
exposure to aerosolized allergens. Individual concerns should be discussed with Occupational
Health or a personal physician.
Wounds: Training in proper handling and restraint of rabbits and rodents is the single most
effective measure in protecting personnel from bites and scratches from these species. Other
than the reported incidence of rate bite fever, the frequency and severity of bites resulting from
rabbits and rodents appears to be minimal. Bite protection gloves may be indicated when
working with rabbits and rodents inoculated with hazardous materials that may be shed in the
saliva, or when working with fractious rodent species. Wearing long sleeves while handling
rabbits can help in avoiding scratches.
Protective Measures: Table 4 outlines recommendations for specific activities associated with
different risks of exposure.
FISH and AMPHIBIAN SPECIES
Zoo notice Disease: Zoonotic diseases associated with fish and amphibian research holding
facilities is infrequent in incidence, but can occur. Table 5 lists select agents transmissible from
fish and frog species to humans that can be of concern.
Allergy: Aerosolized fish proteins can be a source of allergic reactions for people and symptoms
can range from allergic rhinitis to asthma. There also reports of occasional reactions to
amphibious skin and secretions that range from cutaneous to respiratory signs.
Protective measures: Table 6 outlines recommendations for specific activities associated with
different risks of exposure