Biology: Extend and Refine Study Guide Cells 1. Match the organelle with the way it maintains homeostasis in a cell. a. Converts sunlight into chemical energy b. Synthesizes proteins i 1. Golgi Apparatus c. Provides external support g 2. Vacuole d. Digests and recycles materials j 3. Nucleus e. Produces ATP b 4. Ribosome f. Regulates what comes into and out a 5. Chloroplast g. Stores material e 6. Mitochondrion (Particularly large in plants) c 7. Cell Wall h. Houses ribosomes f 8. Cell Membrane i. Packages materials prior to being d 9. Lysosomes exported out of the cell h 10. Endoplasmic Reticulum j. Houses DNA 2. In the diagram below, list the differences and similarities between prokaryotes and eukaryotes: Prokaryotes No nucleus Small Simple Ex. Bacteria Eukaryotes Cell membrane DNA/RNA Ribosomes Cell wall Larger Complex organelles Ex. Plants, animals, fungi, protists 3. Give a comprehensive reason explaining why water moves into and out of cells. (Consider hypertonic, hypotonic, and isotonic solutions.) Water moves into and out of cells in order to help organisms maintain homeostasis, a condition in which the internal environment maintains equilibrium or stability with the external changes by controlling what substances may enter or leave the cell. In isotonic solutions the concentration of water is equal on both sides of the cell membrane (the amount of liquid leaving the cell is equal to that going in to the cell). In hypertonic solutions the strength of the solution is above strength or concentrated, meaning that there is less water outside of the cell so that the water will leave the cell to maintain balance, causing the cell to shrink, shrivel, or die. In hypotonic solutions, the amount of water is higher outside the cell than it is inside the cell causing the water to move into the cell in order to maintain balance. The cell will swell and may burst or lyse. 4. Explain how the cell membrane regulates the movement of salt, water, and other materials into and out of the cell. Passive Transport No Energy Required (Movement of molecules from an area of high to an area of low concentration) Diffusion Molecules move Osmosis Water moves Active Transport Energy Required (Movement of molecules from an area of low to an area of high concentration) Endocytosis Entering the cell Exocytosis Exiting the cell Facilitated Diffusion Protein channels aid in Movement Ion Pump Energy is required to move particles from an area of low concentration to an area of high concentration. 5. Explain why plants, bacteria, and animals often die when exposed to excessive amounts of saltwater. Plants, animals, and bacteria often die when exposed to excessive amounts of salt-water because the saltwater solution is hypertonic to the cell. The water from the cell would leave the cell and enter the solution in order to maintain homeostasis with this external environment, causing the cell to shrivel and perhaps die. (The great amount of energy required for this process (active transport) causes animals/plants to die) 6. Describe experiments that could be used to demonstrate osmosis. One experiment that could be done would be to take an egg without the shell and submerge it into three different solutions (saltwater, freshwater, and sugar water) to watch the movement of liquid and the affect each solution had on the cell. Another experiment would be to water a plant with saltwater solution and watch the effect it would have on the plant. (hypertonic solution) A third would be gargling with saltwater. (Hypertonic solution) A red blood cell in different solutions (hypertonic, isotonic, and hypotonic) Biochemistry 7. Macromolecules: Fill in the chart below. Macromolecule Function Carbohydrate Store (Quick) Energy Monomers Saccharide Found In Starch Sugar Glucose Characterisitics Complexity changes according to the number of monomers attached Lipid Store (Long term) Energy Fatty Acid Oils Fats Steroids Cell membrane Wax Hydrophobic (does not dissolve in water) Protein Enzymes, Antibodies, Hormone receptors, Neurotransmitters, Hair, Nails and Muscle structure, Metabolism Store Genetic Information Amino Acids Meats Nuts Milk Provides structure to organisms (Muscles, etc..) Nucleic Acid Nucleotides Nucleus DNA RNA Strands with Ribosugar backbone Direct Protein Synthesis 8. Enzymes: a. Enzymes are defined as biological catalysts, because they accelerate chemical reactions in living organisms. b. How could you test the effectiveness of an enzyme as a function of: i. Temperature: If the temperature gets too hot, the enzyme changes shape or denatures. ii. pH: Changes in pH may not only affect the shape of an enzyme but it may also change the shape or charge properties of the substrate so that the substrate cannot bind to the active site. 9. Glucose’s major function in a cell is to store chemical energy until the cell goes through respiration. Respiration goes on in the mitochondria as it converts glucose’s energy into the cellular energy molecule, ATP. Energy of Life 10. Plants use light energy to make glucose molecules that will be used later in their cells for cellular respiration 11. What is the energy molecule for cells? ATP 12. What do plants take in and produce during photosynthesis? Take in: Carbon dioxide CO2 Water H2O Produce: Glucose C6H12O6 Oxygen O2 Note: Cellular respiration is the opposite! 13. What happens to ATP when it releases energy in cellular processes? The third phosphate breaks off RNA, DNA, Viruses 14. Compare sexual reproduction to asexual reproduction: Sexual Meiosis Genetic Variability Gametes Asexual Reproduction Produce offspring Mitosis Exact Copies Somatic Cells (body) 15. Compare and contrast the benefits of sexual and asexual reproduction. Sexual Asexual Genetic variability Keep good characteristics Tend to survive as a species When you have something better with variety good, keep it! 16. Compare the characteristics of bacteria and viruses: Bacteria Reproduce independently Both Respiration (metabolism) Cell membrane Viruses Evolve Diseases DNA/RNA Reproduce in a host Not Both No respiration (metabolism) Capsid Much smaller 17. Compare the general characteristics of DNA and RNA. DNA 2 strands Double helix Template for protein synthesis (makes an mRNA) Thymine Nucleic acid Codes for information Guanine Adenine Cytosine RNA 1 strand Carries the code for protein synthesis Uracil 18. Protein Synthesis: a. What molecule is used as the template for the code on what protein to produce? DNA b. How does the genetic code get to the point where protein synthesis occurs? mRNA carries the code to the ribosome c. What do the following terms have to do with protein synthesis? Circle the one that happens first. i. Transcription 1st Makes the mRNA from the DNA In nucleus ii. Translation Makes the protein (amino acid sequence) from the mRNA code At ribosome 19. Describe the steps of replication and explain why this should occur prior to mitosis. Basic Genetics 20. What causes certain characteristics to be inherited by offspring? For example, two heterozygous parents have children who all have the dominant characteristic. What would explain this? A dominant allele will guarantee its characteristics (phenotype) will show up. A recessive phenotype requires two recessive alleles. (Genotypes) In this example, all of the offspring received a dominant allele. 21. A fly with stripes is crossed with a fly having no stripes. (Stripes is Dominant) All of the offspring have stripes. What is the expected stripe pattern (with or without) of the offspring of these heterozygous striped flies? S S s Ss Ss s Ss Ss 100% would have stripes 22. Two pea plants with the genotype TtRR are crossed. What are the probabilities of producing offspring with the genotypes: a. Ttrr 0% b. TTRR 25% c. ttRr 0%