NZQA Expiring unit standard
17620 version 4
Page 1 of 3
Title
Explain basic principles of chromatography and applications of
HPLC techniques in the dairy industry
Level
4
Credits
6
Purpose
People credited with the unit standard are able to explain basic
principles of chromatography separation and explain specific
applications of HPLC chromatography separation techniques in
the dairy industry.
Classification
Dairy Manufacturing > Dairy Laboratory Methodology
Available grade
Achieved
Entry information
Critical health and
safety prerequisites
Open.
Explanatory notes
HPLC in this unit standard refers to High Performance Liquid Chromatography.
Outcomes and evidence requirements
Outcome 1
Explain basic principles of chromatography separation in the dairy industry.
Evidence requirements
1.1
Chromatography separation is explained in terms of type and relevant
applications.
Range
1.2
types may include but are not limited to – gas chromatography,
HPLC, thin layer chromatography, size exclusion chromatography,
gel permeation chromatography.
Evidence is required for a minimum of two types.
Chromatography separation is explained in terms of accurate interpretation of
data and avoidance of limitations, and the assessment of systems suitability.
Range
interpretation and avoidance of limitations – isomers, related
compounds;
Primary Industry Training Organisation
SSB Code 101558
 New Zealand Qualifications Authority 2016
NZQA Expiring unit standard
17620 version 4
Page 2 of 3
systems suitability – height equivalent theoretical plate count,
resolution, plate symmetry.
1.3
Chromatography equipment components are explained in terms of essential
and practical requirements for accurate analysis.
Range
1.4
essential requirements – polarity, flow rate, pressure;
practical requirements – waste processing, elution, gradient
elution, ambient versus elevated oven temperature.
Methods used for signal measurement are identified and explained in terms of
limiting factors in the conversion of signals to quantifiable results.
Range
methods of signal measurement – ultraviolet/visible, flame
ionisation, spectro flurometric, electron capture;
limiting factors – symmetry, superposition, retention times,
resolution.
1.5
The use of internal standards is explained in terms of purpose and interpretation
of variance.
1.6
Column structure is explained in terms of separation and causes and remedies
of common problems.
Range
separation – mechanism, reverse phase, normal phase;
common problems – back pressure, variable pressure, dead
volumes, column wear.
Outcome 2
Explain specific applications of HPLC chromatography separation techniques in the dairy
industry.
Evidence requirements
2.1
HPLC applications are explained in terms of fit with, and result variations from,
traditional methods.
Range
applications may include but are not limited to – vitamin analysis,
lactose analysis.
2.2
HPLC separation is explained in terms of the need to clean up milk component
matrices.
2.3
HPLC separation and analysis is explained in terms of commonalties and
differences in procedure and detection for fat soluble versus water soluble
vitamins.
2.4
HPLC separation and analysis of lactose is explained in terms of identification
and correction of profile variations between product batches.
Primary Industry Training Organisation
SSB Code 101558
 New Zealand Qualifications Authority 2016
NZQA Expiring unit standard
17620 version 4
Page 3 of 3
This unit standard is expiring. Assessment against the standard must take place by
the last date for assessment set out below.
Status information and last date for assessment for superseded versions
Process
Version Date
Last Date for Assessment
Registration
1
25 November 2000
31 December 2017
Revision
2
13 June 2003
31 December 2017
Rollover
3
26 January 2007
31 December 2017
Review
4
15 October 2015
31 December 2017
Consent and Moderation Requirements (CMR) reference
0022
This CMR can be accessed at http://www.nzqa.govt.nz/framework/search/index.do.
Please note
Providers must be granted consent to assess against standards (accredited) by NZQA,
before they can report credits from assessment against unit standards or deliver courses
of study leading to that assessment.
Industry Training Organisations must be granted consent to assess against standards by
NZQA before they can register credits from assessment against unit standards.
Providers and Industry Training Organisations, which have been granted consent and
which are assessing against unit standards must engage with the moderation system that
applies to those standards.
Requirements for consent to assess and an outline of the moderation system that applies
to this standard are outlined in the Consent and Moderation Requirements (CMR). The
CMR also includes useful information about special requirements for organisations wishing
to develop education and training programmes, such as minimum qualifications for tutors
and assessors, and special resource requirements.
Primary Industry Training Organisation
SSB Code 101558
 New Zealand Qualifications Authority 2016