Kutzler et al.
MI-14-355
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Supplemental Fig. 1: Genetic diversity of constructs and immunization schedule. (a)
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Schematic of the immunization and challenge regiments. RhMs were immunized on weeks 0, 6,
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12, 18 and 48. After a 26 week rest, RhMs were challenged with multiple low dose SIVsmE660
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swarm. (b) Genetic tree of SIV sooty mangabey Envelopes. The consensus envelope was made
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using multiple different SIVsm envelopes. The open blue triangles show sequences from the
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SIVsmE660 swarm stock used in the experiment. The red square represents the consensus
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envelope used for immunization.
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Supplemental Fig. 2: Cytokine production from CD4+ and CD8+ T cells after 4th
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immunization. Intracellular cytokine staining was performed on PBMCs isolated at week 21 and
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stimulated with peptides from Gag and Pol and totaled for CD4 (circles) and CD8 (triangles).
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Bars indicate median.
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Supplemental Fig. 3: Peripheral cellular responses do not correlate to challenge outcome or
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peak viral loads. The percent of CD4+ (left) and CD8+ (right) T cells which secreted cytokines
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after stimulated with Gag and Pol peptides was determined two weeks after 4th immunization (a)
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and two weeks after final immunization (b) and plotted against peak viral load. There is not a
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strong correlation between cellular responses, peak viral loads or challenge outcomes.
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Supplemental Table 1: Genotype analysis of RhMs and challenge outcome. Genotype
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analysis was performed on each RhM to insure it did not affect challenge outcome. RhMs were
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grouped based on challenge outcome for all groups including naïve RhMs. Two RhMs in the
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naïve group did not have TRIM5α analysis performed on them and have been not been included
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in the table.
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