BEAUMONT HOSPITAL
DEPARTMENT OF HAEMATOLOGY
Laboratory User Guide
November 2011
Prepared by: Geraldine Healy
Approved By: Dr. Philip Murphy
Dr. John Quinn
Dr. Patrick Thornton
Revision 2: November 2011
Review Date: December 2012
Document Number: HAEMG-LP-038
Page 1 of 21
CONTENTS
Introduction ..............................................................................................................3
Location of the Haematology Department ............................................................3
Contacting the Haematology Department .............................................................3
Clinical Advice and Laboratory Test Interpretation ...........................................4
Laboratory Opening Hours ....................................................................................4
Out-Of-Hours Services ............................................................................................4
General Sample Collection & Labelling Guidelines: In-House...........................5
General Sample Collection & Labelling Guidelines: External ...........................7
Specimen Containers ...............................................................................................8
Transport ..................................................................................................................8
Sample Rejection Criteria .......................................................................................9
Storage.....................................................................................................................10
Guidelines for Haematology Specimens: .............................................................11
Guidelines for Haematology Specimens referred to External Hospitals ..........12
Guidelines for Flow Cytometry Specimens .........................................................13
Guidelines for Routine Coagulation Specimens..................................................14
Guidelines for Non-Routine Coagulation Specimens .........................................15
Guidelines for Coagulation Specimens referred to External Hospitals ...........17
Coagulation Reference Ranges ............................................................................19
Haematology Reference Ranges ...........................................................................20
Test Results .............................................................................................................21
Document Number: HAEMG-LP-038
Page 2 of 21
Introduction
This user guide is designed to enable medical staff to obtain the maximum benefits
from the services provided by the Haematology Department. It provides
information on the tests performed both in the laboratory and those referred to
other centres.
Location of the Haematology Department
The Haematology Department is located on the lower ground floor of Beaumont
Hospital within the Pathology block.
Contacting the Haematology Department
Haematology Office- Enquiries
Reception- Results
01-8092655
01-8092669
Medical Enquiries
Consultant Haematologists
Dr. P. Murphy
Dr. J. Quinn
Dr. P. Thornton (Mon/Wed/Fri only)
01-8093382
01-8092664
01-8093382
Haematology Clinic-Coleman K. Byrne (CKB)
01-8092150/2622
Scientific Enquiries
Chief Medical Scientist
Mr. Karl O’Regan
01-8092662
Senior in Charge of Coagulation
Ms. G Healy
01-8092657
Haematology Laboratory
Coagulation Laboratory
Flow Cytometry Laboratory
Morphology
Special Haematology
Out-Of-Hours
01-8092703
01-8092656/2663
01-8092763
01-8094776
01-8093226
Bleep 252
Pathology Reception-Appointments
Hospital Reception
Warfarin Clinic
01-8092674
01-8093000
01-8092083/3982
Document Number: HAEMG-LP-038
Page 3 of 21
Clinical Advice and Laboratory Test Interpretation
Haematology clinical advice can be obtained by contacting:
 The CKB unit at 8092150/2622.
 The Haematology Registrars at bleep 887/ 276
 The Haematology Senior House Officers at bleep 347/ 490/ 461
Interpretation of Laboratory Tests / procedures may be obtained by phoning the
Chief Medical Scientist or by requesting a senior member of staff.
Laboratory Opening Hours
 The routine opening Hours of the Haematology department are:
Monday-Friday 8am – 8pm
 Please ensure samples arrive in the laboratory as early as possible in the
working day.
 There is no routine Saturday, Sunday or Bank Holiday service.
Out-Of-Hours Services
 The Medical Scientist on call may only be contacted by bleeping 252.
 The following emergency tests will be processed
- Full blood count (FBC)
- Coag screen / INRs /APTTs
- D-Dimer
- Fibrinogen
- Infectious Mononucleosis (IM) screen
- Malaria screen
- ESR (only where temporal arteritis is suspected)
- Sickle screen (urgent theatre cases only)
- Other tests in special circumstances may be arranged with the
Laboratory.
 Send Specimens by means of the pneumatic tube system (2703/2656)
 If a test is urgent hand deliver it to the Laboratory, and notify the Medical
Scientist on-call.
Document Number: HAEMG-LP-038
Page 4 of 21
General Sample Collection & Labelling Guidelines: In-House
 In-house samples must be labelled with the order-com label, (which must be
placed over the existing bottle label). A list of the appropriate test abbreviations
can be found in the tables present in pages 8-15 of this manual.

Thrombophilia Screen (TPSC) requests will no longer be able to
be ordered on PIPE. In order to process a TPSC request, all requests must
first be discussed with the Haematology SpR, Haematology Registrar, or
consultants (Dr. Philip Murphy, Dr. John Quinn or Dr. Patrick Thornton)
available via the hospital switchboard.
If sanctioned, please do the following:
 Complete a Coagulation Downtime Request Form with the Patient's
name, DOB, Episode Number, Ward/Location and Thrombophilia Screen
(TPSC) requested
 Include patient relevant clinical information on the form
 Indicate on the form the name and bleep number of the Doctor on the
Haematology team who has sanctioned the TPSC
 Date, sign and include the requesting Doctor's bleep number
 Take FOUR 2.9 mL Tri-sodium citrate 9NC (green) samples and
 Take ONE 2.7 mL EDTA (pink) sample
 Send the samples to the Coagulation Laboratory
Please note: Anti Cardiolipin Antibodies are processed by Immunology
and are not part of this screen.
TPSC's which have NOT been sanctioned by the Haematology Team will be
rejected and not processed by Scientific Staff.
For more details please read the `Thrombophilia Guidelines' which can be accessed
through `Haematology Guidelines' on the Intranet under `Medical Quicklinks'.
 Urgent samples. Due to the large number of samples received daily with the
order-com label marked as STAT, it is not possible to process each of these as
urgent samples. Therefore it is imperative that if a sample is urgent, the
laboratory must be notified by either telephoning the laboratory staff with the
urgent sample details or hand delivering the sample directly to the laboratory.
Samples from Oncology and CKB must have a blue cap placed over the lid of
the sample to be prioritised.
 Blood Film Requests: Please phone the Haematology Lab if a blood film is
required on an FBC, specifying the clinical indication for this request.
 Add-On D-Dimer Requests: Please phone the Coagulation Lab to prioritise
this request
Document Number: HAEMG-LP-038
Page 5 of 21
 During Computer Downtime: the Haematology Request form (HAEMG-LF007) and/or the Coagulation Request form (HAEMC-LF-026) must be correctly
filled out for in-house samples. The forms must include the following details:
1. Patient name
2. Date of Birth and/or Current episode number
3. Test required
4. Ward/area patient is located
5. Dr. Signature
The sample must be labelled with at least two unique identifiers, one of which
must be the patients’ name.
A hardcopy of the result will be sent either by Chute or porter collection to the
requesting ward, which must be filed in the patient’s chart.
Document Number: HAEMG-LP-038
Page 6 of 21
General Sample Collection & Labelling Guidelines: External
 GP samples must be accompanied by a fully completed Beaumont Hospital GP
request form, to include the following details:
The following details must be recorded on the request form:
 Name of Patient
 DOB
 Practice Name/Address
 Requesting Clinician
 Tests requested
The following details should also be recorded on the request form:
 Patients Address
 An Episode number if available
 Gender
 Date of collection/time
 Drawing doctor’s or phlebotomist’s signature
 Clinical details (where appropriate/relevant-required for ESR)*
 Contact number
The following details must be recorded on specimen:
 Name
 DOB
 Date and time of specimen collection
The following details should be recorded on specimen:
 Drawing doctor’s or phlebotomist’s signature
 ESR is clinically indicated in the following circumstances only:
 Temporal Arteritis/Polymyalgia
 Connective Tissue Diseases
 Lupus
In all other cases, C-Reactive Protein (CRP) is the preferred test.
 HbA1c is analysed in the Biochemistry Laboratory. Patients requiring a FBC
and HbA1c will require 2 EDTA 2.7mL samples sent with the test request.
Document Number: HAEMG-LP-038
Page 7 of 21
Specimen Containers
The Sarstedt monovette system is the specimen collection system in use in
Beaumont Hospital.
Cap
Contents
Application
Volume
Purple Tri-sodium citrate 4NC
Pink
EDTA
ESR only
3.5 mL
Most general Haematology 2.7 mL
Flow cytometry
1.2 mL- Paediatric
PCR
Green Tri-sodium citrate 9NC
Coagulation
2.9 mL
1.2 mL- Paediatric
Red
0.82mg Magnesium/mL Haematology
2.7mL
Orange RPMI & Sodium-Heparin Flow Cytometry
2mL
White No anti-coagulant
Haptoglobin assay
2.7ml or 7.5ml
Transport
 Transport specimens to the laboratory as quickly as possible.
 Place all specimens in bio-hazard bags and transport to the laboratory in a way
as to minimise damage or risk of leakage.
 Samples may be sent via the AV tube system (to 2703/2656) or can be collected
by Porters in the wards at defined times.
Collection times (in-house) are as follows: Monday- Friday collections at 10.00, 12.00, and 15.00
Saturday-Sunday collections at 9.00, 11.00, and 13.00
Collection times (GPs): Each GP on the courier schedule has collections at least
twice weekly.
Exception to the above: Platelet Function Assay (PFA 100)
 The PFA 100 test must be tested 30 mins to 4 hrs after collection and
transported via the porters to the laboratory. Do not use the chute system as it
will interfere with the platelets and consequently the results.
 This test will only be carried out if prior arrangements have been made with the
Coagulation Laboratory.
Document Number: HAEMG-LP-038
Page 8 of 21
Sample Rejection Criteria














Unlabelled / Incorrectly labelled specimens i.e. without two unique patient identifiers
Leaking or improperly sealed containers.
Overfilled: ESR samples where blood is collected into the lid of the container. Coagulation
containers where the blood is filled beyond the marking on the label.
Underfilled: ESR samples must be 85mm full. 2.7 ml FBC and RETFBC samples must have
a minimum of 1mL of blood. 1.2 ml paediatric samples should have at least 500µL for FBC
(up to first indentation of the lid) and at least 1mL for RETFBC. Coagulation samples must
be filled to the line.
Obvious inadequacy of specimen for the test(s) required i.e. only one coagulation specimen
for a Thrombophilia screen
Clotted samples with the exception of a serum clotted sample which can be used for IM and
Haptoglobin testing.
Incorrect anti-coagulant: Samples in the wrong container for the requested test.
Any samples where the laboratory has been informed the specimen was collected from the
incorrect patient.
Haemolysed Coagulation specimens and ESR samples
10mL and 7.5mL EDTA samples for FBC and RETFBC analysis. These samples are not
compatible with the laboratory cap piercing analysers.
8.2 mL sodium citrate samples for Coag screens, INR’s or WINR’s. These samples are
not compatible with the Coagulation analysers.
All in-house TPSC's which have NOT been sanctioned by the Haematology Team will be
rejected and not processed
Aged Samples:Coagulation samples must be less than 8 hours old. Samples greater than 8 hours old the
clotting factors begin to deteriorate which lead to inaccurate results.
INR/WINR samples must be less than 24hrs old.
ESR samples should be less than 8 hours old. Samples greater than 8 hours can lead to a false
lowering of results.
Reticulocyte samples must be less than 6 hours old. Samples greater than 6 hours old can
lead to an incorrect result.
Flow Cytometry samples for lymphocyte analysis must be less than 48 hours old and stored
at room temperature. Samples for white blood cell precursor analysis must be taken in
Sodium Heparin and RPMI and be less than 72 hours old and stored at room temperature.
FBC: EDTA samples must be <48hours
Blood film preparation: samples must be <12 hours old
D-Dimer: Request for D-Dimer received >8 hours post sample collection.
Note for External Users:
 ESR requests which do not have the appropriate clinical information will be rejected.
 In the event whereby 1 EDTA sample is received for FBC and HbA1c analysis, the
HbA1c will be given priority and the FBC request rejected.
In the case of an in-house sample being rejected, the requesting ward will be informed by
telephone and a record of the rejected sample will be kept in the laboratory.
Note: Only External INR requests that are rejected will be telephoned.
Document Number: HAEMG-LP-038
Page 9 of 21
Storage
Samples are stored for one week at room temperature (15-25ºC), after testing, with
the exception of ESRs, which are discarded after reporting. Additional requests
may be added to the original request as follows:
 Reticulocyte count may be added following receipt of a FBC sample but must
be requested within 6 hours of specimen collection to produce accurate results.
 Blood film analysis may be added following receipt of a FBC sample but must
be requested within 12 hours of specimen collection to produce accurate results.
 Depending on the time of collection of the FBC and the stability of the assay,
the following tests may be added to an FBC sample: IM, G6PD, Sickle screen,
PK, HB_El.
 A Malaria screen can be added onto an FBC within 1 hour of collection.
 Fibrinogen and D-Dimers may be added following receipt of a coagulation
sample but must be requested within 8 hours of specimen collection to produce
accurate results
Document Number: HAEMG-LP-038
Page 10 of 21
Guidelines for Haematology Specimens:
Test
Container
Required
Volume /ml
Sample
Full
Blood count
EDTA (pink
capped)
Platelet Check*
0.82mgMg2+/mL
2.7ml standard
In-house: 2-4 Hours
7.5ml and 10ml EDTA
1.2 ml paediatric External: 1 Working Day samples not acceptable
Urgent: 1 hour
2.7mL
In-house: 2-4 Hours
Arrange in advance with
External: 1 Working Day laboratory to obtain sample
tube.
ESR
Trisodium citrate 3.5 ml Must be
4NC /3.5 (purple) filled to the line
Reticulocyte
Count
Haptoglobins
Infectious
mononucleosis
Screen
Blood film
examination
G6PD Screen
Malaria
Expected
Further Information
Turnaround Time
FBC
PLTEXACT
(Can only be
ordered in the
laboratory)
Addressograph label must
only be placed over the
manufacturer’s label on the
bottle.
EDTA (pink
2.7ml standard
In-house: 2-4 Hours
7.5ml and 10ml EDTA
capped)
1.2 ml paediatric External: 1 Working Day samples not acceptable
Urgent: 1 hour
Clotted
7.5ml standard
2.5 Working Days
EDTA samples no longer
sample (white)
2.7 ml paediatric
acceptable (August 2011)
EDTA (pink
2.7ml standard
1 Working Day
capped) Or clotted 1.2 ml paediatric
sample (white)
ESR
EDTA(pink
capped)
Must be requested
by phoning the
Laboratory directly
or on GP request
form
EDTA(pink
capped)
EDTA(pink
capped)
Document Number: HAEMG-LP-038
1 Working Day
Urgent for Temporal
Arthritis: 1 hour
BHIS
Code
2.7ml standard
1-3 Working Days
Sample must be <12 hrs old
1.2 ml paediatric With the exception of the
weekend and films
referred to the
Consultant for Review.
2.7 ml Standard 5 Working Days.
Refer to page 19 for G6PD
Quantitation.
2.7 ml Standard 1-2 hrs for Rapid
When RDT is negative,
Diagnostic Tests(RDTs) films will be processed on
Blood films 4 hours to the next working day
next working day
depending on results of
Page 11 of 21
RETFBC
HP
IM
G6_PD
M_PARA
Test
Container
Required
Sickle solubility EDTA (pink
capped)
Screen
Fresh EDTA
Paroxysmal
(pink capped)
Nocturnal
Haemoglobinuria
Screen
Bone marrow
Bone
marrow aspirate aspirate on glass
Volume /ml
Sample
Expected
Further Information
Turnaround Time
2.7ml Standard
RDTs
1 Working Day
2.7ml Standard
1 Working Day
A minimum of 5
slides.
Stained for next Working Slides must be labelled in BMA
Day. Await Consultant pencil with the patients’
reporting.
Surname and second unique
identifier either D.O.B or
episode number.
slides. Needles and
slides available in
Haematology.
BHIS
Code
For Urgent pre- Anaesthetic SICK_SC
screen contact Laboratory.
Arrange in advance with
Laboratory personnel.
Sample must be freshly
drawn
PNH
Guidelines for Haematology Specimens referred to External Hospitals
Test
Specimen required Referral Laboratory
Plasma Viscosity
1 X EDTA sample
2.7 ml
1 X EDTA sample
2.7 ml
1 X EDTA sample
2.7 ml
1 X EDTA sample
2.7 ml
Haemoglobin
Electrophoresis
HbA2
St James Hospital,
Special Haematology
Laboratory,
Dublin 8.
Test
Mnemonic
VISC
Hb_EL
HbA2
Phone No.’s:
01-4162909 (Plasma
G6PD Deficient
G6_PD
Viscosity)
Samples
01-4162394 (Hb_EL, HbA2,
for Quantitation
Positive Sickle Screens 1 X EDTA sample G6PD, PK)
Hb_EL
2.7 ml
1 X EDTA sample Fax No.: 01-4103513
Pyruvate Kinase
PK
2.7 ml
Document Number: HAEMG-LP-038
Page 12 of 21
Turnaround Time
Verbal reports available
after one week
Written reports
after 6 weeks
Guidelines for Flow Cytometry Specimens
Test
Container
Required
Vol /ml Expected
Further
sample Turnaround Information
Time§
CD4
EDTA (pink capped)
2ml
2 Working days Samples must be <48 CD4
hours old
Lymphocyte Subsets
EDTA (pink capped)
2ml
Lymphoproliferative Panel
EDTA (pink capped)
2ml
2 Working days Samples must be <48 LY_SUB
hours old
4 Working days Samples must be <48 LY_PRO
hours old
Acute Leukaemia Panel
Sodium Heparin
(orange capped)
with 1ml RPMI
2-3ml
Paroxysmal Nocturnal
Haemoglobinuria
Fresh EDTA
(pink capped)
Document Number: HAEMG-LP-038
BHIS
Code
2 Working days Must be arranged in AL
advance with prior
consultation with the
lab. Containers are
only obtained from the
lab.
2.7ml
2 Working Days Arrange in advance
PNH
Standard
with Laboratory
FLOW
personnel. Sample
must be freshly drawn.
Page 13 of 21
Guidelines for Routine Coagulation Specimens
Specimen
Container
Required
Coagulation Screen
Coag Screen (UFH)
Prothrombin Time
Activated Partial
Thromboplastin Time
INR
Trisodium citrate 9
NC/2.9 mL
(green capped)
Warfarin office INR
D-Dimer
Fibrinogen
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Document Number: HAEMG-LP-038
Number of Vol /ml Expected
samples
sample Turnaround
required
Time§
1
1
1
1
1
Must be In-house: 2-4Hours
filled to the External: 1 working Day
line
Urgent: 1 hour
Must be In-house: 2-4Hours
filled to the External: 1 working Day
line
Urgent: 1 hour
Must be In-house: 2-4Hours
filled to the External: 1 working Day
line
Urgent: 1 hour
Must be In-house: 2-4Hours
filled to the External: 1 working Day
line
Urgent: 1 hour
Must be In-house: 2-4Hours
filled to the External: 1 working Day
line
Urgent: 1 hour
Page 14 of 21
Further
BHIS
Information Code
COAGSC
COAGHEP
PT
APTT
INRs only are INR
stable for 24 hrs
Warfarin Office WINR
contact no.
01-8092083
Sample must be D-DIMER
<8 hours old
Sample must be FIBN
<8 hours old
Guidelines for Non-Routine Coagulation Specimens
Specimen
Container
Required
Mixing study
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Factor Assays
Number of Vol /ml Expected
Further Information BHIS
samples
sample Turnaround
Code
required
Time
2
2
Thrombophilia screen Trisodium citrate 9
NC/2.9 mL
(green capped)
Protein C
Antithrombin III
Activated protein C
resistance
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Document Number: HAEMG-LP-038
4
1
1
1
Must be 1 week
Sample must arrive in
MS
filled to the
Laboratory before 4 pm.
line
Must be Case dependent, Tests done in batches. For FII, FV,
filled to the maximum
urgent requests, contact the FVII, FVIII,
line
14 days
laboratory in the morning, FIX, FX,
may be able to facilitate FXI, FXII
testing that day.
Must be 2-3 weeks
Batch tested.
TPSC
filled to the
The Thrombophilia
Cannot order
line
screen (TPSC) includes
on PIPE.
the following tests: PT,
Use a
APTT, FIBN, D-DIMER, downtime
LA, AT3,PC, FPS, APCR form, MUST
and
discuss
5LEIDENand PT_MUT. clinical
Hence, these
details with
Tests do not need to be
Haematology
Ordered on an individual team first.
basis.
Must be 2-3 weeks
Batch tested.
PC
filled to the
Patient must be off
line
warfarin for a minimum of
2wks to perform this assay.
Must be 2-3 weeks
Batch tested
AT3
filled to the
line
Must be 2-3 weeks
Batch tested
APCR
filled to the
line
Page 15 of 21
Specimen
Container
Required
Number of Vol /ml Expected
Further Information BHIS
samples
sample Turnaround
Code
required
Time
Von Willebrand factor Trisodium citrate 9
Lupus anticoagulant
Thrombin time
Reptilase time
Platelet function
analyser (PFA-100)
Factor V leiden
PT mutation
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
Trisodium citrate 9
NC/2.9 mL
(green capped)
EDTA sample
EDTA sample
(pink)
EDTA sample
(pink)
2
1
1
1
1
Must be Case dependent,
filled to the maximum 14
line
days
Must be 2-3 weeks
Batch tested
filled to the
line
VWF
Must be 2 weeks
filled to the
line
Must be 2 weeks
filled to the
line
Must be 4 hours
filled to the
line
TT
EDTA & trisodium citrate
samples are required. These
must NOT be sent in the AV
tube system as this may activate
the platelets. They MUST be
sent to the lab by the porters.
1
6-8 weeks
1
Only tested if APCR is
positive.
6-8 weeks
LA
Order on a
downtime
form
PFA
5LEIDEN
PT_MUT
1
Same day turnaround times refer to results being available to the requesting clinician on the same working day.
Results are available on ward look-up or on Healthlink. Clinicians receiving results by post will incur an added
delay.
Document Number: HAEMG-LP-038
Page 16 of 21
Guidelines for Coagulation Specimens referred to External Hospitals
Test
Specimen required
Referral Laboratory
Test
Turnaround Time
Mnemonic
ADAMTS 13 Assay 2 fresh coag samples <4hrs old should be Haemostasis Research Unit, Hand written Note: Only processed to
centrifuged immediately. Frozen plasma Haematology Dept,
request
facilitate storage in aliquots immediately in the -70ºC freezer University College London,
70ºC freezer, do not get
st
1 Floor, 51 Chenies Mews,
reports back
London, WC1E 6HX, UK
00442076796416
Coag sample taken 4hrs post-heparin Coagulation Laboratory,
tested
every
Anti-Xa Assay
ANTI XA Batch
administration. This can be sent fresh if within St. Vincent’s Hospital,
Friday
4 hours of collection. Double spin, separate and Elm Park,
Written reports: 4-6
freeze plasma aliquots in the -20ºC freezer if Dublin 6.
weeks.
being left over night.
01-2094395
Verbal report available
Note: St Vincent’s must be phoned prior to
within 2 days.
the sample being sent. The type of LMWH must
be written on the request form.
Double spin, separate and freeze plasma Coagulation Laboratory,
aliquots in the -20ºC freezer.
NCHCD,
1 clotted sample, 1 coag sample, 1 vial of Rialto Gate,
HIT Screen
(Heparin-Induced heparin the patient was on. Stable un separated St. James’ Hospital,
Thrombocytopenia) @ room temperature for a few hours or must be Dublin 8.
frozen overnight., if they cannot be sent that 01-4162956
FXIII
Platelet Aggregation Contact must be made by the Haematology
team at Beaumont with the Haematology
Studies
team at St. James’ Hospital. The referral of
the patient must be discussed by both
teams.
Ristocetin CoFactor 2 freshly drawn coag samples, sent
immediately or separated & frozen in the
(RICOF)
-20ºC freezer.
PLTAGG
FXIII
HIT
reports:
4-6
reports:
5
Verbal report available
within 2 days.
day.
Note: ideally the patient must not be on heparin
and the APTT has returned to normal.
Document Number: HAEMG-LP-038
Written
weeks.
Written
weeks.
By appointment only.
reports:4-6
VWF_RCO Written
weeks
Verbal report available
within 5 days.
Page 17 of 21
Test
Specimen required
Referral Laboratory
Test
Turnaround Time
Mnemonic
(drug) Whole blood is not suitable, separated St. Thomas’s Hospital,
WDL
plasma or serum required.
The haemophilia centre,
1ST Floor, North wing,
Lambert Palace Rd,London
SE17EH.
This test is extremely sensitive to pre Biomnis,
Plasminogen
PAI-1
Activator Inhibitor analytical conditions. A freshly drawn coag Three Rock Road,
sample must be mixed immediately by Sandyford Business Estate,
gentle inversion at least six times following Sandyford,
collection.
Must
be
centrifuged Dublin 18
immediately after collection. Frozen 01-2958545
immediately and kept frozen until analysis.
Freshly drawn coag samples, double spun,
Free Protein S
FPS
separated & frozen in the -20ºC freezer.
Warfarin
Level
Document Number: HAEMG-LP-038
Page 18 of 21
2-3 weeks
2 Weeks
2-3 Weeks
Coagulation Reference Ranges
Test
APCR
APR
APTT
AT3
COAGSC
D-Dimer
FII
FV
FVII
FVIII
FIX
FX
FXI
FXII
FVL
FIBN
INR
LA
MS
PFA 100
PT
PT_MUT
PC
RT
TPSC
TT
VWF
WINR
Normal Range/result
Negative
1.5-2.5
24-38
80-120
See PT and APTT
<0.5
60-140
60-140
60-140
60-200
60-140
60-140
60-140
60-140
Negative
1.5-4.5
Different ranges depending on the
reason the patient was put on Warfarin.
Negative
Corrected to within the PT and APTT
normal ranges.
CEPI<164
Units
Ratio
seconds
U/dL
μg/mL
U/dL
U/dL
U/dL
U/dL
U/dL
U/dL
U/dL
U/dL
g/L
Ratio
CADP<116
12-15
Negative
70-140
<20
See individual requests APCR, PC,
FPS, AT3 and LA.
<22
43-150
Therapeutic Range is dependent on
clinical condition
seconds
seconds
U/dL
Secs
Document Number: HAEMG-LP-038
seconds
secs
U/dL
Ratio
Page 19 of 21
Haematology Reference Ranges
Test
FBC
ESR
HP
IM
M_PARA
PNH
RETFBC
SICK_SC
Test
parameters
Normal Range/Result Units
Male
Female
13-17.5
11.5-16.5
Hb
0.37-0.54 0.335-0.54
PCV
4-6.5
3.8-5.8
RCC
79 -96
MCV
27 -32
MCH
12.1-14.3
RDW
140 -400
PLTS
4.0 -11
WBC
Neut 2.0 -7.5
Lymph 1.0 -4.0
Mono 0.2- 1.0
Eosin 0.04- 0.4
Baso 0.01- 0.1
1- 12
1-20
0.45-2.43
NEGATIVE
NEGATIVE
NEGATIVE
RETIC %
0.2 -2.0
RETIC(Abs) 20 - 80
NEGATIVE
Document Number: HAEMG-LP-038
g/dL
L/L
x1012/L
fL
pg
%
x109/L
x109/L
x109/L
x109/L
x109/L
x109/L
x109/L
mm/hr
g/L
%
x109/L
-
Page 20 of 21
Test Results
 Results when authorised, are available on the BHIS / PIPE.
 GP results are also available on Healthlink.
 Results falling outside defined alert limits will be telephoned to the appropriate
ward/ personnel.
 Note: This may not be possible due to an inability to contact the relevant
clinical personnel out of hours. In such cases, the critical alert value will be
telephoned the following day.
The Following Table is a list of these results that will be phoned:
Table: Critical /Action Limits
Test
Hb
PLT
Neutrophils
IM
Malaria Screen
Sickle Screen
INR/WINR
D-Dimer
Fibrinogen
Flow Cytometry
Morphology
Ward/ OPD
GP/
Nursing
Homes/
External Hospitals
<7.0 g/dL
<7.0g/dL
9
<20 x 10 /L
<50 x 109/L
<0.5 x 109/L
<0.5 x 109/L
Positive
Positive
Positive
Positive
Positive
Positive
>5.0
>8.0 (Mon-Thurs) >5.0 (Fri)
>20µg/mL
>5µg/mL
<1.0 g/L
<1.0 g/L
New Leukaemia patients New Leukaemia patients
When Haematology team Printed report sufficient, no
suggests referral contact action necessary.
the clinical team. If
patient discharged, bring
to attention of
Haematology secretary to
contact GP.
Note: It is imperative that contact details of the requesting doctor and/or location of the patient
are attached to the test request so that critical results can be phoned immediately.
Document Number: HAEMG-LP-038
Page 21 of 21