Impact of Digestive and Oropharyngeal decontamination on the
Intestinal Microbiota in ICU Patients
Robin F Benus 1,2,3, Hermie J Harmsen 1, Gjalt W Welling 1, Rob Spanjersberg 2, Jan
G Zijlstra 2, John E Degener 1, and Tjip S van der Werf
3
Departments of 1 Medical Microbiology, 2 Critical Care, and 3 Internal Medicine, and
Pulmonary Diseases & Tuberculosis, Infectious Diseases & Tuberculosis Service;
University Medical Centre Groningen, University of Groningen, the Netherlands
Correspondence:
Dr TS van der Werf, t.s.van.der.werf@int.umcg.nl
Hanzeplein 1, POBox 30001, AA11, room U3.105, 9700RB Groningen
Tel. +31 50 3611501 Fax +31 50 3619320
Supplemental Data:
Target
Probe/stain Sequence
reference
Total bacteria
EUB338
5’GCTGCCTCCCGTAGGAGT
Amann [1]
Bacteroides / Prevotella
Bac303
5’CCAATGTGGGGGACCTT
Manz [2]
E. rectale / Blautia
Erec482
5’GCTTCTTAGTCA(G/A)GTACCG
Franks [3]
F. prausnitzii-group
Fprau645
5’CCTCTGCACTACTCAAGAAAAAC
Suau [4]
Atopobium-group
Ato291
5’GGTCGGTCTCTCAACCC
Harmsen [5]
Bifidobacteria
Bif164
5’CATCCGGCATTACCACCC
Langendijk [6]
Ruminococci
Rbro730
5’TAAAGCCCAGYAGGCCGC
Harmsen [7]
Rfla729
5’AAAGCCCAGTAAGCCGCC
Rint623
5’TTCCAATGCAGTACCGGG
Hold [8]
Rint helper
5’GTTGAGCCCCGGGCTTT
Aminov [9]
Enterobacteriaceae
EC1531
5’CACCGTAGTGCCTCGTCATCA
Poulsen [10]
Enterococcus faecalis
Enfl84
5’CCTCTTTCCAATTGAGTGCA
Waar [11]
Enterococcus faecium-
Enfm93
5’GCCACTCCTCTTTTTCCGG
Waar [11]
coccoides
Roseburia cluster
group
References
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