Emmanuel Mongin (McGill Centre for Bioinformatics, McGill University)
Titre : Prediction, analysis and functional characterization of vertebrate long range
enhancers
Résumé : We computationally predicted a set of Teleost specific regulatory modules
(CRM) using a method developed in our laboratory (Blanchette & al, 2006). We used
Oryzias latipes (Medaka), as reference genome to build regulatory regions. CRM
construction is based on both co-occurrence of the same matrix through the same window
and its conservation with other organisms. About 90 000 putative Medaka CRM were
found. In order to validate our predictions we transfected into Medaka fishes some of
these CRMs. Starting with CRMs conserved with human we showed that 90% of the
predicted CRMs trigger specific expression in Medaka embryos. We present here the
method developed for large-range enhancer prediction as well as the biological
framework established to test these regions in Medaka fish.