Mark-Recapture Lab Determining the Size of an Animal Population Background: Scientists can determine the number of animals in a large population by using a sampling technique. This is a laboratory that simulates a population census technique commonly used by wildlife biologists in the field. Here is how it works. The first step is to trap a random sample of animals of the desired species. These animals are then ear-tagged or marked in some other manner and released. The next step is to trap once again. Some of the animals captured may have been marked from the first sample. Scientists then calculate the total population for their specified area based on the ratio of marked animals to unmarked animals. In this activity, a bag represents the area of land where the population study is being conducted. Elbow Macaroni will represent animals. All the Elbow Macaroni in the bag represent the total animal population being studied. Purpose/Objective: To estimate the population by using a sampling technique Materials: Plastic bag Elbow Macaroni (including 20 marked Elbow Macaroni) Calculator Procedure: 1) Shake your bag of Elbow Macaroni (the marked Elbow Macaroni represent previously caught and marked animals). Without looking into the bag, reach in and remove 30 Elbow Macaroni. 2) Record the number of marked Elbow Macaroni (recaught and marked) and the number of unmarked Elbow Macaroni (caught and unmarked) in your data table as trial 1. 3) Return all the Elbow Macaroni to the bag. 4) Repeat steps 1 to 3 four more times (for trials 2 to 5). 5) Calculate averages for each of the columns. 6) Using the average values, calculate the original size of the bean population in the bag by using the following formula: M = number initially marked CwM = average number caught during the trials with marks Cw/oM = average number caught during the trials without marks Calculated Population Size = M x (CwM + Cw/oM) CwM 7) Record the calculated population size in the data table. 8) Verify the actual population size, count the total number of Elbow Macaroni in the bag and record this value in the data table. Data/Results: Bag # _______ Trial Total caught Number caught with marks (CwM) Number caught without marks (Cw/oM) 1 2 3 4 5 Averages Calculated population size = _________ Actual population size = ________ Discussion: 1) Compare the calculated to the actual population size. Explain why they may not agree exactly. 2) What changes to the procedure would improve the accuracy of the activity? 3) Explain why this technique is used more often with animals than with plants when calculating population size. 4) What species of animal would this technique work well for? What species of animal would this technique not work for? Why? 5) Assume you were doing this experiment with living animals. What would you be doing before the first step? In the first step?