Comparative Genomics Practical
Platelet-derived growth factor receptors (PDGFR)
Platelet-derived growth factor (PDGF) is one of a variety of growth factors that regulate cell
division and growth. In particular, PDGF plays a role in blood vessel formation or
angiogenesis. The different isoforms of PDGF activate cellular response through receptors
including PDGF receptor alpha (PDGFRa) and beta (PDGFRb).
The goals of this practical are as follows:
1. Explore the genomic location of PDGF receptor alpha and beta.
2. Speculate on the evolutionary origins of these receptors.
3. Understand how the UCSC genome browser works.
1. Identifying PDGFR on the UCSC genome browser
Search the UCSC genome browser (http://genome.ucsc.edu/cgi-bin/hgGateway) using the
term “PDGFR”. The genome browser returns a list of genomic locations for genes that match
your search query. Click on the link to RefSeq genes that correspond to PDGRF alpha and
beta. Remember: you will need two browser open on your desk top so that you can compare
the alpha and beta genes.
1a. PDGFR alpha maps to chromosome ________
1b. PDGFR beta maps to chromosome _________
2. Navigating the genome browser
Use the zoom out buttons on the top right-hand corner to refresh the graphic display.
Zooming out will display more genes in the same graphics window.
2.1 Downstream neighboring gene for PDGFR alpha = _____________
2.2 Downstream neighboring gene for PDGFR beta =________________
2.3 Do the PDGFR genes and their corresponding neighbors share any protein classification?
2.4 What implications, if any, do your answers above together with the genomic location of
these genes have on the origins of the PDGFR genes?
3. Comparative Genomics Track
Zoom out until you only have the PDGFR alpha gene in full view. Scroll down till
you see the “comparative genomics” tracks. Set the “conservation” track to “full”.
3.1 Which 4 species do not show any conservation with human PDGFR-alpha from
position 54800000 – 54810000.
3.2 Explain the following terms that are used to describe the level of detail on a
“track”?
3.2.1 full
3.2.2. dense
3.2.3 squish
3.2.4 pack
3.3 Hide the “conservation” track and scroll to the “Expression and Regulation”
section. Set the “TFBS_conserved” track to “full”. List the two TFBS that are located
at the “most” three prime end of the PDGFR alpha gene.
4. ENSEMBL GENOME BROWSER (www.ensembl.org)
Click on the human division of ensembl and search for PDGFR.
4.1 Identify the ENSEMBL ProteinID for PDGFR Beta.
4.2 Click on the ProteinID and find a summary report of the different domains for the
protein. One of the tracks shows a “SMART domain”. What database does
“SMART” refer too?
4.3 What specific domain(s) is/are reported from the SMART database.