J5OwlPel OWL PELLETS INTRODUCTION Owl pellets are the undigested remains of prey ingested by an owl. The owl swallows its prey whole, and during the process of digestion, the soft parts of the prey are dissolved and passed on to the intestine for absorption. The hard, non-digestible parts-bones, teeth, fur, feathers, and chitonous remains of insects are compressed in the gizzard and passed on to the proventriculus, the first chamber of the owl’s stomach, where the pellet remains until it is expelled. These pellets are not eliminated as feces, but are regurgitated through the mouth. Pellets are not found exclusively within the owl families. There are many species of birds known to regurgitate pellets: hawks, eagles, kites, harriers, falcons, and even robins are some of the more familiar ones. Out of all types of pellet ejectors, the efficiency of the process is probably as high in owls as in any other bird. The Common Barn Owl feeds in early morning and early evening and will usually produce one to two pellets per day. Glossy black when fresh, the pellet remains smooth and dark in color when dry. Each pellet has been fumigated to eliminate the presence of any insects and then individually wrapped for preservation. Unless otherwise stated, the pellets in this lab are from the family Tytonidae and more specifically, the Common Barn Owl (Tyto alba). SAFETY 1. Be careful, teasing needles are very sharp. They are not toys. 2. Wash hands before leaving the lab. MATERIALS owl pellet 2 teasing needles forceps glue hand lens paper plate rulers balance bone sorting charts PROCEDURE Part 1: Pellet dissection 1. Work in pairs or as directed by the teacher. 2. Remove the pellet from the aluminum foil. 3. Mass the pellet and record the mass in Table 1. 4. Measure the length and width of the pellet and record these measurements in Table 1. 5. Place the pellet in a paper plate. Carefully separate the bones from dried fur or feathers using a teasing needle and forceps. Place the bones in another part of your plate. 6. Carefully clean the bones and sort them according to type, skulls, jaws, vertebrae, etc. 7. When you are sure that all bones have been removed from the fur, you may discard the fur and/or feathers. 8. Identify the animals found in your pellet comparing your skulls to the skulls in the picture keys. 9. Using a dot of glue, attach each bone beside the matching bone on the diagram on your lab sheet. You will sometimes have many of each type of bone and they will all be glued beside the diagram. 10. Record the types and numbers of animals in Table 2 and in the chart on the board. 11. When the last group has recorded their data on the board, copy the class data in Table 2. Revised 8/09 ASIM Biology: Environment Page 1 of 12 Owl Pellets - J5OwlPel Part 2: Ecological Pyramids The amount of energy or matter in an ecosystem can be illustrated by a drawing called an ecological pyramid. In this activity you will construct three types of ecological pyramids: a number pyramid, a biomass pyramid and an energy pyramid. Each pyramid you construct will consist of four trophic levels. The top of each will relate to your owl, the only tertiary consumer (3o). The next level down will relate to the owl’s prey, the secondary consumers (2o). For the purposes of this activity, assume that all of the prey found in the owl pellets are secondary consumers. The next trophic level down will relate to the organisms eaten by the owl’s prey, the primary consumers, (1o). The base of each pyramid will relate to the organisms eaten by the primary consumers, the producers. Use the information obtained from your owl pellet dissection to construct the ecological pyramids below. Number Pyramid, for one 24 hour period A number pyramid is an ecological pyramid that indicates the total number of organisms in each trophic level 1. Draw your pyramid, labeling the number of 3o, 2o and 1o consumers. (Reminder: There is only 1 owl, which is the 3o consumer, and you have calculated the average number of prey found in each pellet for your class.) 2. Determine the ratio, R, of the number of 2o consumers to the number of 3o consumers. R = # 2˚ consumers # 3˚ consumers 3. Use your ratio, R, to calculate the number of 1o consumers by multiplying the number of 2o consumers by R. Label your pyramid with the number of 1o consumers. # 1o consumers = R (# 2˚ consumers) 4. Calculate the number producers by multiplying the number of 1o consumers by the ratio, R. Finish your pyramid by labeling it with the number of producers. # producers = R ((# 1˚ consumers) Biomass Pyramid, for a 2.5 year lifespan A biomass pyramid is an ecological pyramid that indicates the total mass of organisms in each trophic level 1. Draw your pyramid and label the total biomass of the 3o consumer, assuming the owl’s biomass is 500 g. Label the biomass of the 2o consumers. (Reminder: You have already calculated the average biomass eaten over a 2.5 year lifespan for your class) Revised 8/09 ASIM Biology: Environment Page 2 of 12 Owl Pellets - J5OwlPel 2. Determine the new ratio, R, of the total biomass of the 2˚ consumers to the total biomass of the 3˚ consumers. R = biomass of 2˚ consumers biomass of 3˚ consumers 3. Use your ratio, R, to calculate the total biomass of the 1˚ consumers by multiplying the total biomass of the 2˚ consumers by R. Label your pyramid with the total biomass of the 1˚ consumers. biomass of 1˚ consumers = R (biomass of 2˚ consumers) 4. Calculate the total biomass of the producers by multiplying the total biomass of the 1˚ consumers by R. Do you see a pattern in your calculations? Finish your pyramid by labeling it with total biomass of the producers. Energy Pyramid An energy pyramid is an ecological pyramid that indicates the amount of energy in each trophic level. According to the terms of thermodynamics, only about 10% of the energy available within one trophic level is transferred to organisms at the next trophic level. 1. Assume that the producers in this pyramid, plants, contain a total of 500,000 Calories of food energy. Label the base of your pyramid with this value. 2. Calculate the amount of energy for the 1˚ consumers by multiplying the food energy of the producers by 0.10. Write this value in your pyramid. 3. Complete your energy pyramid by calculating and labeling the energies for the top two trophic levels. Revised 8/09 ASIM Biology: Environment Page 3 of 12 Owl Pellets - J5OwlPel Revised 8/09 ASIM Biology: Environment Page 4 of 12 Owl Pellets - J5OwlPel Revised 8/09 ASIM Biology: Environment Page 5 of 12 Owl Pellets - J5OwlPel Revised 8/09 ASIM Biology: Environment Page 6 of 12 Owl Pellets - J5OwlPel Identifying Owl Pellet Contents* How to measure the jaw Tooth types Jaw Length Revised 8/09 ASIM Biology: Environment Lobed Angled Pointed Page 7 of 12 Owl Pellets - J5OwlPel Rat Vole Mouse Shrew Bird Jaw Length (mm) 17-30 15-20 10-15 7-14 15-40 Tooth Type Lobed Angled Lobed Pointed None Skull and Jaw Shoulder Blade Hip Upper Leg Lower Leg Rib Back Bones Foot Revised 8/09 ASIM Biology: Environment Page 8 of 12 Owl Pellets - J5OwlPel STUDENT DATA SHEET OWL PELLETS Name:_______________________ Date: _______________________ DATA Part 1 Table 1. Owl Pellet Measurements Mass Length Width Table 2. Kinds and Numbers of Animals Found in Owl Pellets Vole Shrew Bird Your Data Class Data Mole Rat ANALYSIS 1. Construct a bar graph from the classroom data collected above. 2. Assuming that the barn owl regurgitates one pellet per day, how many prey items would the owl that produced your pellet consume per year? 3. During the nesting season the young need an enormous amount of food for growth. If the nest contains three young and each of the young eat five mice per night for a month and the two adults eat four per night, how many mice would the parents have to capture in 30 days? Revised 8/09 ASIM Biology: Environment Page 9 of 12 Owl Pellets - J5OwlPel 4. Environmentalists are concerned about the accumulation of certain poisons in predators. Why do you suppose owls, eagles, swordfish, humans and lions are often the most threatened organisms of a community that are exposed to DDT or mercury? 5. Placing the owl at the highest trophic level, create a food web using the following items: vole, deer mouse, mole, house mouse, weasel, shrew, snake, starling, frog, salamander, spider, grubs, earthworms, centipedes, crane fly, seeds, plants, roots. 6a. What valuable information do you think can be gained by studying the owl pellet? b. List other information that can be gained by studying owl pellets. 7. What are owl pellets? 8. What happens to the soft parts of the prey? 9. What is the purpose of the gizzard in the owl pellet formation? 10. Where does the pellet remain until it is expelled? 11 These pellets are not eliminated as _________________, but are regurgitated through the ___________________. Revised 8/09 ASIM Biology: Environment Page 10 of 12 Owl Pellets - J5OwlPel 12. List 6 other birds that are known to regurgitate pellets. 13. Of all the birds that are pellet ejectors, which type of bird is probably the most efficient at this process? ________________________________ 14. How many pellets does the common barn owl produce per day? ________________________ 15. What color are the pellets when fresh? ____________________________________________ 16. Describe the appearance of a dry owl pellet. 17. The pellets are from what owl family? _____________________________________________ 18. Why are the pellets fumigated? 19. How do food webs and food chains differ? 20. Why does the number of organisms decrease as you move up the pyramid level? 21. In terms of energy flow in an ecosystem, why is the amount of energy passed from one trophic level to the next so small? Since we know energy cannot be destroyed, account for the loss of energy that occurs between the prey and the owl. 22. We know that about 50% of the energy in an organism is not digested by the consumer and is simply passed through as waste or feces. Is the energy stored in the feces lost to the ecosystem? Why or why not? Revised 8/09 ASIM Biology: Environment Page 11 of 12 J5OwlPel Part 2 Table 3 B C D E= D/# of grps Biomass Number found in pellet Total number found by class Average number found per pellet for class A PREY TYPES House Mouse 20 g Vole 40 g Shrew 5g Brown Rat 240 g Bird 80 g F= E x 365 Average number eaten per year (1 pellet per day:365 days) G= BxE H= G x 365 I= H x 2.5 Average biomass eaten per 24 hours (g) Average biomass eaten per year (365 days) (g) Average biomass eaten over a 2.5 year lifespan (g) Other Total Drawings of pyramid of number Revised 8/09 Drawing of biomass pyramid ASIM Biology: Environment Drawing of energy pyramid Page 12 of 12