Peptide Microarray Service
Project Description Form
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Please complete as much of this form as possible as we will use this
information to design your experiments. (Note: for items left blank, we will
insert information based on our successful experiences. No experiments
will begin prior to your approval.)
LC Sciences Use Only
Detailed information about your sample is critical for a successful assay.
Date Received:
(888) 528-8818
(713) 664-8181
www.lcsciences.com
LC Sciences Project #:
Email this form to sales@lcsciences.com. We will contact you to finalize
details of the experiment(s).
Name:
Phone:
Institution:
Email:
[Part 1 – Objectives]
Describe the main objective(s) of the project:
[Part 2 - Peptide Microarray Design & Contents]
Source of Sequences:
Format (Check One):
Accession #:
Tiling (AA Increment
)
Customer Design (See Below)
Other Describe:
Maximum Length of peptides:
(standard up to 8mer):
Design Instructions (if applicable):
Exceptions (exclusions of synthesis):
[Part 3 - Analyte/Sample Description & Assay Conditions]
Sample Name:
Type of Molecule:
Accession #:
Protein
Antibody
DNA
RNA
Enzyme
Cell Lysate
Serum
Other
Mw (kDa):
Sample Source (organism, isolation
method):
Sample Description (if applicable):
Length (AA)
Isoelectric Point:
Dissoc. Constant (Kd):
Functional Tags (e.g. His, GST, Fluor
Dye, tec.):
* Alternate Blocking Buffer:
Components:
pH:
Fold Dilution Required:
Dilution Buffer:
Components:
pH:
Fold Dilution Required:
Binding Buffer:
Components:
pH:
Fold Dilution Required:
Analyte/Sample Concentration (ng/ml):
(standard up to 8mer):
Binding Reaction:
Time:
* Alternate Washing Buffer:
Components:
Washing:
Time:
Temperature:
pH:
Fold Dilution Required:
Temperature:
Volume:
Additional Remarks:
* We will use LC Sciences proprietary blocking buffer and washing buffer unless you suggest an alternate.
© 2007 LC Sciences LLC CF-30-02A
Peptide Microarray Service – Project Description Form
[Part 4 - Detection]
Method of Detection:
Direct detection (dye labeled sample)
Dye Molecule :
Through dye labeled analyte/protein-specific antibody
Through primary antibody and dye labeled secondary antibody
Analyte/Protein-Specific Antibody (if applicable)
Antibody Name:
Accession #:
Antibody Type (e.g. IgG, IgM, etc):
Antibody Immunotype (e.g. anti-GST):
Antibody Raised From (e.g. rabbit):
Antibody Specific For:
Antibody Binding Constant (Kd):
Dye Molecule (if applicable):
Secondary Antibody (if applicable)
Suggested Secondary Antibody:
Secondary Antibody Specific for what
Species:
Dye Molecule:
Polyclonal
Monoclonal
FAb
Single-chain
Mw (kDa):