BIOLOGY REPRODUCTION and DEVELOPMENT Topic 7: The Secret of Life: Nucleic acids 1. What are the 2 main functions of nucleic acids? 2. Write the name of components of a nucleotide given below. 4. Two nucleotides that on each strand bind with __________ bond and two nucleotides that on the same strand bind with the ______________ bond. 5. The two strands of a DNA double helix are antiparallel. This means that _____. A) the two strands are mirror images B) only one of the two strands can be used as a template for replication, since DNA polymerase only works in one direction C) one strand is actually composed of RNA D) one strand runs in the 5’ --> 3’ direction and the other runs in the 3’ --> 5’ direction E) they both run in the 3’ --> 5’ direction 6. One strand of a DNA molecule has the base sequence ATAGGT. The complementary base sequence on the other strand of DNA will be _______________. 3. What we call the units (monomers) which make up the chains of RNA and DNA? A) Monosaccharides B) Disaccharides C) Amino acids D) Fatty acids E) Nucleotides 4. Which of the following lists the four bases contained in DNA? A) cytosine, guanine, thymine, uracil B) adenine, guanine, hemanine, thymine C) guanine, pyroline, thymine, uracil D) adenine, guanine, purine, thymine E) adenine, guanine, cytosine, thymine 7. Which one of the following accurately reflects complementary base pairing in the DNA molecule? A) guanine–cytosine B) guanine–adenine C) cytosine–hemanine D) uracil–thymine E) adenine–cytosine 8. Which of the following is NOT true about doublestranded DNA? A) It is helical. B) It contains phosphodiester linkages. C) The two strands are said to be complementary. D) Adenine and uracil are present in equal amounts. E) The strands run antiparallel. 9. What is the function of Phosphodiester bonds in DNA structure? 5. Which of the following bases is the purine base? A) Thymine B) Cytosine C) Uracil D) Pyroline E) Adenine 6. What is the main difference between DNA and RNA? Write at least 3 differences. Topic 8: DNA 1. What are the functions of DNA in cell? Write 3 functions. 2. A DNA molecule includes 2000 nucleotides.There are 300 guanine in this DNA. How many hydrogen bonds are there in this DNA? 10. Which bonds hold together double strands of DNA? 11. A DNA molecule includes 2000 nucleotides. There are 300 Guanines. a) How many cytosines are there? b) What is the sum of Adenines and Thyimines? c) What is the total number of Hydrogen bonds found between Guanines and Cytosine? d) What is the total number of Hydrogen bonds found in this DNA molecule? Answer: 2300 3. One strand of DNA that molecule has a nucleotide sequence TAACGTA. Its complementary DNA strand will have a sequence that reads: A) AUUGCAU B) CGGTACG C) ATTGCAT D) ATGCAAT E) ATTCGAT Date: ____/_____________/ 2011 Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT 12. One strand of DNA has a nucleotide sequence TAACGTA. Its complementary DNA strand will have a sequence that reads: A) AUUGCAU B) CGGTACG C) ATTGCAT D) ATGCAAT E) ATTCGAT 1. Mach the sentences. a) mRNA: b) tRNA : c) rRNA : 1) ____ transport amino acids from cytoplasm to ribosome Topic 9: DNA replication 2) ____ form ribosome’s with proteins 1. Mach the correct sentences with the terms given below. a) DNA ligase b) DNA polymerase c) DNA Helicase d) DNA primase e) RNA primer 3) _____ carry information from DNA to ribosome 1.___unwinds the two DNA strands 2.___Produce RNA primers 3.___builds the new strands in a 5’ to 3’ direction 4.___Links the Okazaki fragments 5.___starts the building of new DNA strands 2. 3’ AATGCGTAT 5’ is the one strand of a DNA molecule. What is the sequence of the leading and lagging strand that produced in DNA replication? 2. AAA TTT CCC GGG is the DNA strand. Which of the following is the mRNA strand produced from DNA given above? A) TTT AAA GGG CCC B) UUU AAA GGG CCC C) UUU TTT GGG CCC D) TTT AAA CCC GGG E) AAA TTT CCC GGG 3. The triplets of nucleotides base on the mRNA are called __________. The triplets of nucleotide base on the tRNA are called ___________. 4. Where can we find the RNA in a cell? 5. Where can we find the DNA in a cell? 3. What is the aim of DNA replication? 4. Replication process occurs every time from ……of new strand to……. 6. Which of the following brings the amino acids from different sides of cytoplasm to ribosome? A) DNA B) tRNA C) mRNA D) mtDNA E) rRNA 5. The DNA strand being produced continuously in the 5’ --> 3’ direction is called the _____ strand. A) Okazaki B) leading C) bubble D) lagging E) new Topic 11: Chromatin and Chromosome 6. Two new strands of DNA molecules grow as bases are added by the enzyme _____. A) bacterial duplicating complex B) DNA polymerase C) DNA replicase D) Okazaki enzyme E) polynucleotidase 2. What components are found in the nucleosome? 7. What enzyme joins Okazaki fragments? 4. Explain the following terms: Histone: 1. What is the chromatin? 3. What is the difference between chromatin and chromosome? 8. Which enzyme unwinds the helical structure of DNA? Nucleosome: 10. Explain the following terms: Chromatin: - DNA Helicase: - DNA Polymerase: - DNA Ligase: Chromatid: 12. In DNA replication short replication parts in lagging strand are called as ………………………. Chromosome: Topic 10: RNA Date: ____/_____________/ 2011 Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT 5. What is the correct sequence from smaller to bigger? a- Chromatin b- DNA c- Nucleotide d- Chromosome e- Gene f- Chromatid g- Nucleosome The correct order: _______________________ E) 46 chromosome are found in the gamete cells 6. An egg cell of human karyotype is symbolized as _______ and a sperm cell symbolized as ___________ or ____________. 7. Why a cell divides into two? Write 2 reasons. 8. A human somatic cell contains _____ chromosomes. Answer: 46 7. A nucleosome composes of __________. Answer: DNA and histone protein 8. The region of a chromosome holding the two double strands of replicated DNA together is called _____. A) chromatin B) a centriole C) a centromere D) a chromatid E) an aster 9. The complex of DNA and protein that makes up a chromosome is properly called _____. A) a chromatid B) a chloroplast C) chromatin D) a chromoplast E) a chromagen Topic 12: Cell Karyotype 1. How many chromosomes are found in a human cell? 9. If an intestinal cell in a grasshopper contains 24 chromosomes, a grasshopper sperm cell would contain ____ chromosomes. Answer:12 10. What are the effects of X and Y chromosomes to human? Topic 13: Mitosis 1._____________ is a period from the beginning of one division to the beginning of the next division 2. DNA replication occurs in ___________. Answer: S phase 5. Explain the following phases of Cell cycle. G1: 2. What is the meaning of karyotype? S: G2: 3. Explain the following terms. Haploid chromosomes: Mitosis: Sister chromosomes: 6. How many chromosomes are present in a somatic human cell in G1? A) 23 B) 46 C) 92 D) 184 E) none of the above Homologous chromosomes: 7. Sister chromatids separate during ___________. A) anaphase B) G1 phase B) G2 phase D) metaphase E) prophase Diploid chromosomes: 4. If an organism has 34 chromosomes, its gamete cells are _________________. A) diploid and have 17 chromosomes. B) diploid and 34 chromosomes C) haploid and have 17 chromosomes D) haploid and 34 chromosomes E) triploid and 51 chromosomes 5. Which of the following is false for human karyotype? A) Have 23 pair of chromosome B) Have 23 homologous chromosome pairs C) Have 44 autosome and 2 sex chromosome D) Contain diploid cells Date: ____/_____________/ 2011 8. In animal cell mitosis, the cleavage furrow forms during ___________. A) anaphase B) G1 phase C) cytokinesis D) metaphase E) prophase 10. If a human muscle cells divide mitotically 4 times, what will be expected the number of daughter cells and their chromosome number? Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT 7. Fill in the blanks in figure given below. 12. Sister chromosomes are separated from each other through the each pole in __________ phase. 15. Which of the following is false about mitosis cell division? A) Produce 2 cells. B) Daughter and parent cell are identical C) Chromosome number remains constant D) Seen in body cells E) It decreases chromosome number to haploid (n). 16. "Cytokinesis" refers to _____. A) division of the entire cell B) division of the nucleus C) division of the cell outside the nuclear material D) reduction in the number of chromosomes E) cell movement Topic 14: Meiosis 1. What is the aim of the Meiosis? 2. How many egg cells are produced at the end of meiosis? 3. Which of the following is not correct for meiosis? A) It reduces the chromosome numbers into half B) Four cells form by one meiotic division C) Occurs only in body cell D) Occurs in reproductive cells E) Involves two divisions 4. Why do cells divide in meiosis two times? 5. Which of the following occurs during anaphase I of meiosis? A) Formation of tetrads B) Separation of homologous chromosomes C) Crossing over D) Separation of sister chromatids, with one chromatid pulled toward each pole E) Line up of chromosomes along the equatorial plane 6. What is the crossing-over? In which phase does it occur? 8. At which stage of Meiosis are chromosomes lined up in one plane in preparation for their separation to opposite poles of the cell? A) prophase I B) metaphase I C) anaphase I D) telophase I E) interphase 9. Which of the following best describes the meiosis? A) It is carried out in all tissues that require cell replacement. B) It occurs only in cells in the reproductive structures of the organism. C) It happens in all tissues except the brain and spinal cord. D) It is the first stage of mitosis. 10. Which of the following is true for meiosis II? A) Sister chromatids separate each other B) Crossing over is observed in prophase II C) Chromosome number increase during the metaphase II D) Meiosis II occurs in somatic cells E) After the meiosis II daughter cells can divide many times again. 11. If an organism receives 12 chromosomes from its father, how many chromosomes can be expected in its brain cells? A) 12 B) 24 C) 64 D) 36 E) 38 12. Rice gamete cells contain 12 chromosomes. How many chromosomes do rice diploid cells have? A) 3 B) 6 C) 12 D) 24 E) 48 13. What is the typical result when a diploid cell undergoes meiosis? A) two diploid cells B) two haploid cells C) four diploid cells D) four haploid cells E) two haploid cells and two diploid cells Date: ____/_____________/ 2011 Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT 14. Mitosis and cytokinesis result in the formation of ______; meiosis and cytokinesis result in the formation of ________. A) four diploid cells ... four haploid cells B) two diploid cells ... two haploid cells C) two diploid cells ... four haploid cells D) two diploid cells ... two diploid cells E) four haploid cells ... two diploid cells 15. What are the differences between Mitosis and Meiosis divisions? 16. Why does chromosome number of cells decrease half of parent cell’s chromosomes? 17. Where do Meiosis divisions occur in the body? 18. What is the effect of crossing-over at the variation of organism? Topic 15: Protein Synthesis 1. Why do we need to synthesize proteins? 2. What is the function of DNA in protein synthesis? 3. Which of following carries information coded in DNA to ribosomes? A) tRNA B) mRNA C) rDNA D) rRNA E) mtDNA A) 4 D) 20 B) 8 E) 23 C) 12 8. How many nucleotides are needed to code for a protein with 450 amino acids? A) at least 150 B) at least 300 C) at least 450 D) at least 900 E) at least 1,350 9. Which of the following is NOT associated with RNA? A) ribose B) thymine C) uracil D) phosphates E) single-strandedness 10. Which of the following is false about protein synthesis? A) before synthesis DNA must be unzipped B) code of RNA are given by antisense strand of DNA C) transcription started by RNA polymerase D) mRNA strand is identical to antisense DNA strand except, instead of thymine to uracil E) tRNA can transfer only one type of amino acid 11. Which of the following codons can not code a functional amino acid? A) UAC B) UAA C) GUG D) AUG E) UGG 12. I- m-RNA is attached to ribosomes II- m-RNA moves from the nucleus to cytoplasm III- t-RNA molecules carring aminoacids arrange themselves along the mRNA template IV- m-RNA separates from the DNA strand along which it was synthesized V- m-RNA separates from ribosome Which of the following represent the correct sequence of the steps listed above, leading to the formation of a protein in a eukaryotic cell? A) IV-III-I-II-V B) I-IV-V-II-III C) I-IV-III-II-V D) IV-I-II-V-III E) IV-II-I-III-V 13. How many amino acids can be synthesized by mRNA formed from one strand of DNA which has 4200 total Hydrogen bond and 300 Adenine in protein synthesis? 4. Explain the terms of transcription and translation. 5. When RNA is being made, the RNA base ____ always pairs with the base _____ in DNA. A) U ... T B) T ... G C) U ... A D) A ... U E) T ... A 6. If the each amino acid is coded by 3 bases (codon) of 4 bases (ATGC), there must be 64(43) amino acid. But we have only 20 kind of amino acid. Write the reason. 14. The DNA code is CGT. What messenger RNA is made from this? A) ATU B) GCA C) TCU D) CTA E) UCG 15. The translation process in eukaryotes requires all of the following, EXCEPT _____. A) ribosomes B) RNA polymerase C) aminoacyl tRNA synthetase enzymes D) transfer RNA E) AUG codons 7. 5' TACAGCGCTGGC 3' The above sequence of DNA is part of a gene. How many amino acids are coded for by this segment? Date: ____/_____________/ 2011 Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT Topic 1: Reproduction: A) Asexual Reproduction 1. What are the types of asexual reproduction? 1. 2. 3. 4. 5. 6. 2. Yeasts reproduce by means of ____________. A) Budding B) Fission C) Spores D) Regeneration E) Sexually 3. What is the name of process given aside? A) Binary fission B) Sporulation C) Regeneration D) Meiosis E) Tissue culture 12. What is the type of reproduction given above? A) Stem grafting B) Stolon C) Rhizomes D) Tuber E) Cutting 13. Which of the following organisms can reproduce by regeneration? A) Bacteria B) Starfish C) Elephants D) Bat E) Lizard 14. What is the meaning of tissue culture? 4. _____________________ is the production of offspring from a single parent by simple division. 15. How is the tissue culture applied? 5. Which organisms can reproduce by means of binary fission? 6. Which of the following organisms can reproduce by spores? A) Fish B) Pine C) Mushroom D) Starfish E) Birds 16. What is the bud grafting? Explain. 7. What is the meaning of vegetative propagation? Topic 2: B) Sexual Reproduction 1. Why does sexual reproduction differ from asexual reproduction? 8. Write the types of vegetative propagation. a) b) c) 9. What is the name of reproduction type given below? 2. Which of the followings is not a type of sexual reproduction? A) Conjugation B) Metagenesis C) Oogamy D) Sporulation E) Heterogamy 3. Metagenesis is the alternation of generations and involves the regular alternation between sexual and asexual reproductive phases during the life cycle of an organism. Which of the following organisms has metagenesis in its life cycle? A) Stem grafting B) Stolon D) Tuber E) Cutting C) Rhizomes A) Mosquito D) Ants 10. Which of the following reproduces by stolons? A) Tomato B) Potato C) Strawberry D) Cherry E) Banana B) Frog E) Bees 4. Write the types of Sexual reproduction due to gametes size? 11. Which of the following can reproduce by means of tubers? A) Melon B) Apple C) Banana D) Potato E) Wheat Date: ____/_____________/ 2011 C) Plasmodium Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT 5. Which of the following organisms can reproduce by parthenogenesis? A) Plant B) Ants C) Tigers D) Fish E) jelly fish Anther: 6. What is the parthenogenesis? Receptacle: Filament: Egg: 7. What is the main difference between Isogamy and Heterogmay? 8. What is the Oogamy? Which organisms have it? 4. Which of the following parts of flowers produces pollens? A) Sepal B) Petal C) Anther D) Pistil E) Receptacle 5. Which of the flower parts surrounds and protects other parts of flower while a flower is developing? A) Sepal B) Petal C) Stamen: D) Pistil E) Receptacle Topic 4: Steps of Plant Reproduction 9. What is the meaning of metagenesis? 1. Order the steps of plant reproduction? 1) Gametogenesis A. B. 2) 3) 4) 5) 6) Topic 3: Plant Reproduction 1. Which of the followings is not an example of nonflowering plants? A) Mosses B) Liverworts C) Pine D) Horsetails E) Fern 2. Label the parts of flower given below. 2. Define the following terms: Spermatogenesis: Oogenesis: Pollination: Ovule: Cotyledon: 3. Explain the spermatogenesis process by drawing. 3. Write the functions of flower parts given below. Sepal: 4. What is the main difference between cross and selfpollination? Petal: Ovary: 5. Why do plants prefer cross pollination? Style: Stigma: Date: ____/_____________/ 2011 Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT 6. Explain the Double Fertilization process that occurs in the flowers. 3. The site of leaf production is termed a/an ______________ and the length of stem in between each is termed a/an __________________ . 4. What is the seed dormancy? 7. Match the structures present at fertilization and the structures they develop into as follows: a- Egg ___ Fruit b- Ovary wall ___ Embryo c- Ovule ___ Seed d- Polar cells ___ Endosperm 8. In the process of pollination, pollen grains are transferred from the _____ to the _____. A) ovary ... Anther B) stigma ... Ovary C) anther ... Sepal D) carpel ... Stigma E) anther ... Stigma 9 Classify fruits according to their structure and give one example for each one. a) b) c) d) 10. Which part of seed forms the root system of plant? A) Testa B) Radicle C) Seed coat D) Endosperm E) Plumule 5. What are the node and internode? 6. Which of the followings is not requirement of the plant germinations? A) The dormancy of seed must be broken B) Water must be present in a sufficient amount to be absorbed by testa C) Light must be present for photosynthesis D) Oxygen must be available for respiration E) The temperature must be high enough for seed enzyme 7. Primary growth refers to __________. A) seed dormancy. B) an increase in the length of plant C) an increase in the diameter of a stem D) growth produced by lateral meristems E) production of fruits 8. The seed contains all of the following Except ______. A) An embryo B) Store food C) Sufficient water for germination D) Integuments E) Seed coat Topic 6: Animal Reproduction 11. Which of the following fruits belongs to the group of accessory fruits? A) Lemon B) Orange C) Melon D) Strawberry E) Fig 12. After fertilization, the _____ develops into a seed and the _____ develops into a fruit. A) ovule ... Ovary B) pollen grain ... Ovule C) ovary ... Ovule D) egg ... Ovule E) egg ... Ovary Topic 5: Plant Development 1. What conditions must be available for respiration of the germinating seed? 1. Explain reproduction in honey bees. 2. Which of the following species of animals reproduces by parthenogenesis? A) Flat worms B) Sea stars C) Hydra D) Honey bee D) Squid 3. How sponges reproduce sexually? 4. What is the meaning of hermaphroditic organisms? Explain it. 2. Which of the following hormones is about control of plant growth? A) Auxins B) Endocrine C) Insulin D) Adrenaline E) Thyroxin Date: ____/_____________/ 2011 Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT 5. In a bee colony or hive, there are three types of members: Worker, Drones and a Queen. The workers and queen are female, whereas the drones are male. Which of these organisms is haploid (n)? A) Drones B) Adult Queen C) Young Female D) Worker E) The larva of fertilized egg Topic 8: Animal Development 1. What is the zygote? 6. Write the gender (sex) of organisms given below in the bee colony. 2. What are the steps of animal development? Queen: 3. What is the aim of fertilization? Drones: Workers: 7. What are the differences of internal and external fertilizations? 8. Which organisms reproduce by external fertilization? 4. How many sperm cells can enter into an egg cell? Why? 5. Which of the following structures is used to drill coat of the egg cell during fertilization? A) Tail B) Acrosome C) Pollen D) Nucleus E) Mitosis Topic 7: Vertebrate Reproductive System 6. Zygote divides repeatedly until 32 cells are formed, this process is known as a ___________. 1. What are the functions of testes and ovary in Vertebrate Reproductive System? 7. Many successive divisions later, a ball of cells forms, known as a ___________. 8. Write the names of stages of animal development to below of their picture? 2. What are the external and internal fertilizations? Give example for each of them. ____________________ _____________ _________________ _________________ 3. Which of the following organisms reproduces externally? A) Chicken B) Whale C) Frog D) Tiger E) Rat 4. How do fish reproduce? 5. The fertilization of an egg by a single sperm forms a _______________. Date: ____/_____________/ 2011 9. In which development process the embryonic layers are produced? A) Blastula B) Fertilization C) Cleavage D) Gastrulation E) Morula Signature: _____________________ BIOLOGY REPRODUCTION and DEVELOPMENT Topic 9: Differentiation and Organogenesis 1. What are the different embryonic layers to form different tissues and organs? 2. Which of the following organs is formed by Mesoderm? A) Brain B) Gland C) Kidney D) Lungs E) Pharynx 4. Which of the following is a watery substance which supports the embryo during embryological development? A) Chorion B) Placenta C) Amnion D) Allantois E) Vitellus 5. Explain the following embryonic tissues. Ectoderm: Endoderm: Mesoderm: 6. Write the across of organs or organ systems that develop from which embryonic tissue. Brain: Ectoderm Bones: ________ Kidney: ________ Liver: ________ Lung: ________ Heart: ________ Spinal Cord: ________ 7. In the given diagram below, process B is known as A) Differentiation C) Spermatogenesis E) Meiosis B) Oogenesis D) Germination Date: ____/_____________/ 2011 Signature: _____________________