UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
Busulfan
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Department:
Date SOP was written:
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This lab specific SOP has been reviewed and approved by:
Principal Investigator Name:
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Principal Investigator Signature
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Type of SOP:
☐ Process
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☒Hazardous Chemical
☐ Hazardous Class
Purpose
Busulfan is a carcinogen. This SOP provides information about its hazards and how to mitigate them through
proper controls, handling, and storage. Busulfan is commonly used to treat chronic myeloid leukemia and in
bone marrow transplantation where it is used as a conditioning drug.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 55-98-1
Class: Select Carcinogen
Molecular Formula: C6H14O6S2
Form (physical state): Powder
Color: White
Boiling point: N/A
Potential Hazards/Toxicity
Carcinogenic, Highly Toxic by Inhalation, Toxic by Ingestion, Highly Toxic by Skin Absorption
Busulfan
1
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Acute toxicity
Oral LD50 - mouse - 110 mg/kg.
Engineering Controls
Work with this chemical in a certified ducted fume hood. Facilities storing or utilizing this material should be
equipped with an eyewash facility and a safety shower.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Nitrile or latex gloves are recommended. Gloves must be inspected prior to use. Use proper glove removal
technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of
contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and
dry hands.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with Busulfan.
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses or goggles are recommended.
Skin and Body Protection
Full length pants, closed-toe shoes, and a lab coat.
Hygiene Measures
Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the
product.
First Aid Procedures
If inhaled
Move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
Busulfan
2
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
In case of skin contact
Wash off with soap and plenty of water for 15 minutes. Take victim immediately to hospital. Consult a
physician.
In case of eye contact
Rinse thoroughly with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids.
Get medical aid immediately.
If swallowed
Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Busulfan
3
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
Busulfan
4
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)