BIOGRAPHICAL SKETCH
NAME
POSITION TITLE
Xiaolian Gao
eRACOMMONS USER NAME
Professor
xgaoxgao
EDUCATION (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
Beijing Institute of Chemical Technology, China
Rutgers, the State University of New Jersey
New Brunswick
DEGREE
YEAR
CONFERRED
FIELD OF STUDY
B. S.
1982
Polymer Science
Ph.D.
1986
Organic Chemistry
NOTE: The Biographical Sketch may not exceed four pages. Items A and B (together) may not exceed two of the four-page limit.
A. Positions and Honors
1989
1989/9-11
1990-1992
1991-1992
1992-1998
19921993-2004
1998-2002
2002-2004
20042004-
Associate Research Scientist, Dept. Biochem. & Biophys., Columbia University
Visiting Scientist, Dept. of Biophysics, Ecole Polytechnique, France
Senior Research Scientist, Dept. Struct. & Biophys. Chem., Glaxo Inc. Research Lab.
Adjunct Assistant Professor, Dept. Biochemistry, Univ. of North Carolina, Chapel Hill
Assistant Professor of Chemistry, University of Houston
Director of the Keck/IMD NMR Center, University of Houston
Adjunct appointment, Biology and Biochemistry, University of Houston
Associate Professor of Chemistry, University of Houston
Professor of Chemistry, University of Houston
Adjunct Professor of Chemistry, University of Houston
Professor of Biology&Biochemistry, University of Houston
1984-1985
1989-1990
1993
1994-1996
1996
2000-2003
2004-2006
2006
2006
2006
2006
Louis Bevier Graduate Fellowship, Rutgers University
Leukemia Society Special Fellowship, the Leukemia Society of America
Junior Faculty Enhancement Award, the Oak Ridge Associated Universities
Junior Faculty Award, American Cancer Society
Research Excellence Award for Assistant Professors, University of Houston
Research Directors, the National Foundation for Cancer Research
NIH BBCA Study Session, member
Sigma Xi Faculty Research Award, the University of Houston (2006)
The AWC Award for Leadership in Technology - the Top Houston Women in Technology (2006)
The Section Award of the American Chemical Society, Greater Houston Section for 2006
the YWCA Outstanding Science and Technology Award (2006)
B. Selected peer-reviewed publications (in chronological order)
1. Stassinopoulos, A., Ji, J., Gao, X., and Goldberg, I. H. (1996) Solution structure of a DNA bulge complex with an
enediyne analog. Science 272, 1943-1946.
2. LeProust, E., Pellois, J. P, Yu, P., Zhang, H., Srivannavit, O., Gulari, E., Zhou, X., and Gao, X (2000) Combinatorial
screening method for synthesis optimization on a digital light-controlled microarray platform. J. Comb. Chem. 2, 355360.
3. Pellois, J. P, Wang, W. and Gao, X. (2000) Peptide synthesis based on t-Boc chemistry and solution
photogenerated acids. J. Comb. Chem. 2, 349-354.
4. Leproust, E., Pearson, C. E., Sinden, R. R., and Gao, X. (2000) The unexpected formation of parallel duplex by
GAA and TTC trinucleotide repeats of friedreich's ataxie. J. Mol. Biol. 302, 1063-1080.
5. Wang, W., Yu, P., Zhang, H., Yang, X., Liang, T.-C., and Gao, X. (2001) Synthesis and characterization of a
tetranucleotide containing alternating phosphonate-amide backbone linkages. Bioorg. & Med. Chem. 9, 107-119.
6. Han, X. and Gao, X. (2001) Sequence specific recognition of ligand-DNA complexes studied by NMR. Current
Medicinal Chemistry. Review. 8, 551-579.
7. LeProust, E., Zhang, H., Yu, P., Gao, X., Xiang, X., and Zhou, X. (2001) Characterization of
oligodeoxyribonucleotide synthesis on glass plates. Nucleic Acids Res. 29, 2171-2180.
8. Gao, X., LeProust, E., Srivannavit, O., Gulari, E., and Zhou, X (2001) Flexible DNA chip synthesis gated by
deprotection using solution photogenerated acids. Nucleic Acids Res. 29, 4744-4750.
9. Nagaswamy, U., Gao, X., Martinis, S. A., and Fox, G. E. (2001) NMR structure of a ribosomal RNA hairpin
containing a conserved pentaloop. Nucleic Acids Res. 29, 5129-5239.
10. Gao, X., Stassinopoulos, A., Ji, J., Kwon, Y., Bare, S., and Goldberg, I. H. (2002) Induced formation of a DNA bulge
by a molecular wedge ligand – Post-activated neocarzinostatin chromophore. Biochemistry 41, 5131-5143.
11. Pellois, J. P., Zhou, X., Srivannavit, O., Zhou, T., Erdogan, G., and Gao, X. (2002) Individually addressable parallel
peptide synthesis on microchips. Nature Biotechnol. 20, 922-926.
12. Cohen, David M., Wei, Jingna, Smith, E. O’Brian, Gao, Xiaolian, Quast, Michael J., and Sokoloff, Louis. (2002) A
Method for Measuring Cerebral Glucose Metabolism In Vivo by 13C-NMR Spectroscopy. Magnetic Resonance in
Medicine, 48, 1063-1067.
13. Kwon, Y., Xi, Z., Kappen, L. S., Goldberg, I. H., and Gao, X. (2003) A New Complex of Post-Activated
Neocarzinostatin Chromophore with DNA. Bulge DNA Binding from the Minor Groove. Biochemistry 42, 1186-1198.
14. Xia, Y., Yee, A., Arrowsmith, C. H., and Gao, X. (2003) 1HC and 1HN Total NOE correlations in a single 3D NMR
experiment. 15N and 13C time sharing in t1 and t2 dimensions for simultaneous data acquisition. J. Biomol. NMR
27, 193-203.
15. Cohen, D. M., Guthrie, P. H., Gao, X., Sakai, R., and Taegtmeyer, H. (2003) Glutamine Cycling in Isolated Working
Rat Heart. Am. J. Physiol. Endocrinol. Metabolism 285, E1312–E1316.
16. Gao, X., Zhou, X., and Gulari, E. (2003) Light directed massively parallel on-chip synthesis of peptide arrays with tBoc chemistry. In situ synthesis and applications. Proteomics, 3, 2135-2141.
17. Gao, X., Gulari, E., and Zhou, X. (2004) In situ synthesis of oligonucleotide microarrays. Biopolymers. 73, 579-596.
18. Gao, X., Pellois, J. P., Kim, K., Na, Y. , Gulari, E., and Zhou, X. (2004) High Density Peptide Microarrays. In situ
synthesis and applications. Molecular Diversity. 8, 177-187.
19. Zhu, G., Xia, Y., Lin, D., and Gao, X. (2004) “TROSY-based correlation and NOE spectroscopy for NMR structural
studies of large proteins” Ch. 5 in Methods in Molecular Biology/Molecular Medicine. Ed. Walker, J. M. 278, 57-78.
Hamans, UK.
20. Zhu, G., Xia, Y., Lin, D., and Gao, X. (2004) “TROSY-based NMR experiments for the study of macromolecular
dynamics and hydrogen bonding” Ch. 8 in Methods in Molecular Biology/Molecular Medicine. Ed. Walker, J. M. 278,
164-184. Hamans, UK.
21. Rouillard, J. M., Lee, W. Truan, G., Gao, X., Zhou, X. and Gulari, E. (2004) Gene2Oligo: Oligonucleotide design for
in vitro gene synthesis. Nucleic Acids Res. 32, W176-180.
22. Li, C., Xia, Y., Gao, X., and Gershon, P. D. (2004) Mechanism of RNA 2'-O-methylation: Evidence that the catalytic
lysine acts to steer rather than deprotonate the target nucleophile. Biochemistry 43, 5680-5687.
23. Xia, Y., Veeraraghavan, S., and Gao, X. (2004) (3,2)D GFT-NMR experiments for fast data collection from Proteins.
J. Biomol. NMR 29, 467-476.
24. Jun, K.-Y., Xia, Y., Han, X., Zhang, H., Timchenko, L., Swanson, S. M., and Gao, X. (2004) 1H, 15N and 13C
Chemical Shift Assignments of RNA Repeats Binding Protein – CUGBP1ab. J. Biomol. NMR 30, 371-372.
25. Zhou, X., Cai, S., Hong, A., Yu, P., Sheng, N., Srivannavit, O., Yong, Q., Muranjan, S., Rouillard, J. M., Xia, Y.,
Zhang, X., Xiang, Q., Ganesh, R., Zhu, Q., Makejko, A., Gulari, E., and Gao, X. (2004). Microfluidic picoarray
synthesis of oligodeoxynucleotides and simultaneously assembling of multiple DNA sequences. Nucleic Acids Res.
32, 5409-5417.
26. Srivannavit, O., Gulari, M., Gulari, E., LeProust, E., Pellois, J. P., Gao, X., Zhou, X. (2004) Design and fabrication of
microwell array chips for a solution-based, photogenerated acid-catalyzed parallel oligonuclotide DNA synthesis.
Sensors and Actuators A. 116, 150-160.
27. Tian, J., Gong, H., Sheng, N., Zhou, X., Gulari, E., Gao, X. and Church, G. (2004) Accurate multiplex gene
synthesis from programmable DNA chips. Nature 432, 1050-1054.
28. Xia, Y., Legge, G., Jun, K.-Y., Qi, Y., Lee, H., and Gao, X. (2005) IP-COSY, a totally in-phase and sensitive COSY
experiment. J. Mag. Chemistry 43, 372-379.
29. Zhao, Q., Nagaswamy, U., Lee, H., Xia, Y., Huang, H.-C., Gao, X., and Fox, G. E. (2005) NMR Structure and Mg2+
binding of an RNA segment that underlies the L7/L12 stalk in the E. coli 50S ribosomal subunit. Nucleic Acids Res.
33, 3145–3153.
30. Mulder, B. A., Anava, S., Yu, Pl, Lee, K. W., Nguyen, A., Murphy, J., Willson, R., Briggs, J. M., Gao, X., and Hardin,
S. (2005) Nucleotide modification at the -phosphate leads to the improved fidelity of HIV-1 reverse transcriptase.
Nucleic Acids Res. 33, 4865-4873.
31. Zhu, Q., Hong, A., Sheng, N., Zhang, X., Jun, K.-Y., Srivannavit, O., Gulari, E., Gao, X., and Zhou, X. (2006)
“Microfluidic Biochip for Nucleic Acid and Protein Analysis” in Methods Mol. Biol. Ed. Rampal, J. B. in press.
32. Vainrub, A., Deluge, N., Zhang, X., Zhou, X., and Gao, X. (2006) “Predicting DNA duplex stability on oligonucleotide
array” in Methods Mol. Biol. Ed. Rampal, J. B., in press.
33. Stähler, P, Beier, M, Gao, X, Hoheisel, J. (2006) “The other side of genomics: Advanced synbio promises to simplify
the exploitation of whole-genome sequence information”. J. Biotec. 124: 206-12.
34. Anbanandam, A., Albarado, D.C., Nguyen, C.T., Halder, G., Gao, X., Veeraraghavan, S. (2006) Insights into
transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain. Proc. Natl. Acad. Sci.
USA. 103, 17225-30.
C. Research Support
Ongoing Research Support
R41 HG003786
Xiaolian Gao (PI)
4/1/06-3/31/08
NIH/GM/HGRI
Parallel DNA Assembling on Microchip
New method development for high throughput long DNA synthesis using microfluidic chip.
10%
R43 GM076941
Xiaolian Gao (co-PI)
4/1/06-3/31/08
NIH/GM/HGRI
Xiaochuan Zhou (PI)
Protein kinase substrate peptide microchip
Developing proteomic peptide chips for profiling and study protein kinases.
10%
R44 GM067364
Xiaolian Gao (PI)
8/1/2003-5/31/2007 (one year extension)
10%
NIH/GM
Development of a peptide microchip system
Development of peptide microfluidic chips for screening of phosphopeptides using STAT3 as a model system.
E1270
Xiaolian Gao (PI)
6/1/03-5/31/06
10%
The Robert Welch Foundation
Parallel synthesis using photogenerated reagents
This proposal is to develop array synthesis of organic compounds using our photogenerated reagent (PGR) chemistry.
Completed Research Support (last three years)
R43 AI 60179-01
Xiaochuan Zhou (PI)
4/16/2004-4/15/2005
NIH/NIAID
Operation site: Atactic Technologies Inc., Houston
Microfluidic real-time PCR array
Develop real time PCR on chip for genetic analysis and quantitative measurements of DNA sequences
N39998-01-C-7071
Xiaochuan Zhou (PI)
1/02-9/03
DARPA
Rapid synthesis of large DNA
Development of methods for assembling 10 kbp DNA sequences using microchip synthesized oligonucleotides
DAAD05-02-C-0038
Xiaochuan Zhou (PI)
1/02-9/03
DARPA
Bioinformatic encoding
Bioinformatic program development for large DNA synthesis and analysis of experimental results
N66001-01C-8065
Susan Hardin (PI)
9/1/01-9/30/04
DARPA
Real-time Sequence Determination
Operation Site: Visigen Biotechnologies, Inc., Houston
Develop technology for real time sequencing of DNA by synthesis and single molecular spectroscopy
Grant # N.A.
Xiaolian Gao (PI)
10/00-9/03
The National Foundation of Cancer Research
Rapid structure elucidation of antitumor agent-DNA/RNA complexes. Towards accelerated discovery of anticancer
therapeutics
Small molecule binding screening of DNA binding and NMR studies
R01 GM49957
Xiaolian Gao (PI)
9/1/99-8/31/04
NIH/GM
NMR and UV studies of antisense oligonucleotides
This proposal is related to NMR studies of the structure and conformation of antisense DNA and RNA duplexes
containing structure modifications.
Grant # N.A.
Xiaolian Gao (PI)
11/01/02-10/31/03
High Performance Computing Inc. (DOD subcontractor)
Database method for rapid and automated determination of protein structures without de novo NMR chemical shift
assignments