Student Directed Studies (click on student name for the study description) Jose Baquerizo (Summer 2010 – present) – TOPO Cloning nNOS cDNA into the pET151 Vector and Examination of Expression Kirsten Bell, Kristen Rose, Kim Akridge (Summer 2009) – Site directed mutagenesis of eNOS and nNOS cDNA for use in studies of NOS function and regulation Laura Couser, Natalie Dinsmore, Isaac Mensah (Summer 2008) – Expression and isolation of neuronal and epithelial nitric oxide synthase from E. coli transformants James Wright and Charles Simpson (Spring – Summer 2008) Blake Smith (Spring – Summer 2007) Deborah Gaddis (Summer 2006) – A Comparison of HPLC and TrisTricine SDS-PAGE Methods for the Detection and Quantification of Phytochelatins in C-Fern Deserah Strand (Summer and Fall 2005; Spring 2006) – Selection and preliminary ion analysis of Ceratopteris richardii mutants tolerant to toxic levels of cadmium; 2-D PAGE Analysis of Proteins in the Wild Type Strain of Ceratopteris richardii In Response to Cadmium Stress Saman Namatbakhsh (Spring and Summer 2005) – Analysis of Phytochelatin Accumulation in the Sensitive Wild Type Strain of Ceratopteris richardii Exposed to Cadmium Stress David Clements (Summer and Fall 2004) – Selection of Cd Tolerant Mutants of C-Fern and a Preliminary Ion Analysis of The Wild Type Strain and Mutant Cdt1 Patrick Shaw (Fall 2003) - An Assessment of Available Molecular Approaches For Use in a Population Genetics Study of KSU's Pink Lady Slipper Population (Cypripedium acaule) Alicia Saran (Spring and Summer 2002) - Selection of Cadmium Tolerant Mutants of C-Fern Kathy Diehl (Summer and Fall 2001) – SDS PAGE Analysis of Light Induced Proteins in Pea Seedlings: A Laboratory Exercise for Plant Physiology Michelle Hall (2000 - 2001) – Determination of Abscisic Acid Content in Water-Stressed Plants Using an Immunoassay: Development of a Laboratory Exercise for Plant Physiology Dominika James and Jason Welch (2000) – The Use of PCR to Generate Molecular Fingerprints of Daylily Cultivars Marc Hogan (1998 – 1999)– Cloning the Betty L. Siegel Daylily Ken Rogers (1998) – The Effects of Mechanical Pruning on Transpiration Rates in Boltonia asteroids Using a Sap-Flow Sensor Andrew Norman (1997) – Assessment of Differential Display PCR To Identify Biomarkers Produced in Response to Permethrin Stress by Coral Reefs Mariam Yazdani and Amber Love (1995) – Documenting the Effect of Cadmium Chloride on Ceratopteris richardii Spore Germination and Gametophyte Growth