Proposals for integration with the 7th Framework Programme
The Second Call 2007 CALL 2A: FP7-KBBE-2007-2A
KBBE-2007-1-1-03: Development of genetic systems for crop improvement through a
system biology approach
INTROGRESSION OF GENETIC MATERIAL OF TRITICEAE TRIBE WILD
SPECIES INTO CULTIVATED CEREALS GENOME
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Phone: (37517) 2841901
Fax: (37517) 2841917
E-mail: L.Khotyleva@igc.bas-net.by
Address: 27 Akademicheskaya St., Minsk 220072, Republic of Belarus
Scientific adviser:
Prof. Dr.Sc. L.V. L.Khotyleva
Members of team:
Ph.D. L.V. Koren
Ph.D. O.A. Orlovskaya
Objective: To develop qualitatively new forms of cultivated cereals by introgressing genetic
material of wild species of Triticeae tribe into their genome, to study expression of alien genes in
a new genetic background.
Work plan:
Task 1: to work out an effective program for using wild spicies of Triticeae tribe for expanding
and improving cultivated cereals gene pool and to develop qualitatively new cereals forms;
Task 2: to estimate genome stabilization in obtained cereals hybrids by cytological methods;
Task 3: to make morphological, biochemical and molecular-genetic analyses for revealing
introgression of alien genetic materials;
Task 4: to study expression of alien genes in a new genetic background, the developed forms and
inheritance of agronomic traits determined by the given introgression;
Task 5: to carry out screening of the obtained lines with introgressions of the genetic material of
wild spicies for the quality, resistance to biotic and abiotic factors; to estimate and to select the
best lines with agronomic traits.
Methods: Application of the remote hybridization method in Triticeae tribe for developing new
cereals forms. Study of the produced hybrid material by cytological, electrophoretic and
molecular-genetic methods. Complex estimation of the produced cereals forms for resistance to
biotic and abiotic factors.
Deliverables: A new way of applying genetic resources of wild populations will be
experimentally substantiated for expanding and improving the gene pool of cultivated cereals.
New data on expression in a new genetic background and their inheritance were obtained. As a
result of the work performed, triticale and wheat forms will be developed which contain the
genetic material of wild species of Triticeae tribe and exhibit complex resistance to pathogens
and have a high protein content in seed. The best forms will be included in breeding programs
for developing cultivars resistant to abiotic and biotic stresses.
Publications:
1. L.N. Kaminskaya, L.V. Koren, E.A. Salina, I.N. Leonova, T.T. Efremova, L.V.
Khotyljova. Expression of Vrn genes in triticale and some their molecular-genetic
aspects. Proceedings of the 5 International Triticale Symposium. Vol. 2. June 30 – July 5,
2002. Radzikow, Poland. pp. 103-110.
2. О.А. Orlovskaya. Analysis of chromosome conjugation in F1 and F4 hybrids during
introgression of Aegilops into triticale genome. Doklady Natsionalnoy akademii nauk
Belarusi, 2002. Vol. 46, N 3. pp. 88-91.
3. I.N. Leonova, O.B. Dobrovolskaya, L.N. Kaminskaya, I.G. Adonina, L.V. Koren, L.V.
Khotyljova E.A. Salina. Molecular analysis of the triticale lines with different Vrn gene
systems using microsatellite markers and hybridization in situ. Russian Journal of
Genetics, 2005. Vol. 41, N 9. pp. 1236-1243.
4. О.А. Orlovskaya, L.N. Kaminskaya, L.V. Khotyljova. Development and analysis of
triticale F1 hybrids containing Aegilops alien material. Doklady Natsionalnoy akademii
nauk Belarusi, 2006. Vol. 50, N 3. pp. 77-81.
5. О.А. Orlovskaya, L.N. Kaminskaya, L.V. Khotyljova. Introgression of Aegilops genetic
material into hexaploid triticale genome. Russian Journal of Genetics, 2007. Vol.43, N 3.
pp. 1-6.