CURRICULUM VITAE
BIOGRAPHICAL
Name: Jay K. Kolls, M.D.
Birthdate:
Home Address: 143 E. Ruelle.
Mandeville, LA
Birthplace: Salisbury, Maryland
Home Phone:
Citizenship: USA
412-736-0033
Business Address:
May 20, 1959
E-Mail Address: jkolls@lsuhsc.edu
LSU Health Science Center
Department of Genetics
CSRB 657
533 Bolivar St
New Orleans, LA 70112
Business Phone: 504-568-6151
Business Fax: 504-568-8500
---------------------------------------------------------------------------------------------------------------------EDUCATION AND TRAINING
UNDERGRADUATE:
Dates
Attended
Name and Location
of Institution
Degree Received
and Year
Major
Subject
Aug. 1977 Ursinus College
B.S. (Cum Laude)
- May 1981 Collegeville, Pennsylvania 1981
Physics
GRADUATE:
Dates
Attended
Name and Location
of Institution
Aug.1981
University of Maryland
– May 1985 School of Medicine
Baltimore, Maryland
Degree Received
and Year
Major Advisor
Subject
M.D.
Medicine
POSTGRADUATE:
Dates
Attended
Name and Location
of Institution
Name of Program Director
and Discipline
Jul. 1985
- Jun.1986
Louisiana State University
Affiliated Hospitals
New Orleans, Louisiana
Internship (combined
Medicine/Pediatrics)
Jul. 1986
- Jun 1989
Louisiana State University
Affiliated Hospitals
New Orleans, Louisiana
Residency (combined
Medicine/Pediatrics)
Jul. 1989
Tulane University Medical Center
- Jun. 1993 New Orleans, Louisiana
Fellowship, Pediatric
Pulmonology
Jul. 1990
Louisiana State University
- Jun. 1993 New Orleans, Louisiana
Fellowship, Adult
Pulmonary Medicine
Jul. 1991
Louisiana State University
- Jun. 1992 Medical Center
New Orleans, Louisiana
Research Fellow,
LSU Section of
Pulmonary/Critical Care
Jul. 1992
Howard Hughes Medical Institute
- Jun. 1993 Laboratory of Dr Bruce Beutler
UT Southwestern
Dallas, Texas
Post-Doctoral
Research Fellow, Immunology
----------------------------------------------------------------------------------------------------------------------
APPOINTMENTS and POSITIONS
ACADEMIC
Years
Inclusive
Name and Location
of Institution or Organization
Jul. 1991
Department of Medicine
- Jun. 1993 Louisiana State University
Rank/Title
Clinical Instructor
2
School of Medicine
New Orleans, Louisiana
Jul. 1993
Department of Medicine
– Jun. 1997 Louisiana State University
School of Medicine
New Orleans, Louisiana
Assistant Professor of
Medicine and Pediatrics
Jul. 1997
Department of Medicine
– Jun. 2000 Louisiana State University
School of Medicine
New Orleans, Louisiana
Associate Professor of
Medicine and Pediatrics
Jul. 2000
Department of Medicine
– Aug. 2003 Louisiana State University
School of Medicine
New Orleans, Louisiana
Professor of Medicine
and Pediatrics
Aug. 2003
- Dec 2008
Department of Pediatrics
Children’s Hospital of Pittsburgh
Pittsburgh, PA
Professor of Pediatrics
and Immunology
Nov. 2006
- Dec 2008
Department of Pediatrics
Children’s Hospital of Pittsburgh
Pittsburgh, PA
Neils K. Jerne Professor of
Pediatrics and Immunology
Jan. 2009present
Department of Genetics
Chair, Professor of Genetics and
Pediatrics
NON-ACADEMIC POSITIONS:
Jul. 1995
- Jun.1997
Children’s Hospital
New Orleans, Louisiana
Member, Pharmacy and
Therapeutics Committee
Jul. 1995-present
Medical Center of Louisiana
New Orleans, Louisiana
Director, Pediatric
Pulmonary Lab
Jul. 1997
- Jun 2000
Children’s Hospital
New Orleans, Louisiana
Director, Pharmacy and
Therapeutics Committee
Jul. 1997
- Jun. 1999
Children’s Hospital
New Orleans, Louisiana
Member, Medical
Executive Committee
3
Jul. 1997
- Aug. 2003
Louisiana State University
HSC
New Orleans, Louisiana
Director, Gene Therapy
Program
Aug. 2003
Children’s Hospital of Pittsburgh
Division Chief,
- present
Pittsburgh, PA
Pediatric Pulmonology
---------------------------------------------------------------------------------------------------------------------CERTIFICATION and LICENSURE
SPECIALTY CERTIFICATION:
Certifying Board
FLEX
ACLS
PALS
American Board of Pediatrics
American Board of Internal Medicine
Pulmonary Medicine
Pediatric Pulmonology
Year
1985
1986
1989
1991
1989
1992
1994, 2002
MEDICAL or OTHER PROFESSIONAL LICENSURE:
Licensing Board/State
Louisiana, 1985 #018641 UPIN# F13287
Pennsylvania: MD422162
Year
1985
2003
---------------------------------------------------------------------------------------------------------------------MEMBERSHIPS in PROFESSIONAL AND SCIENTIFIC SOCIETIES
Organization
Sigma Pi Sigma
American Medical Association
Southern Medical Association
American Academy of Pediatrics
(Candidate Fellow)
American College of Physicians (Associate)
American College of Chest Physicians
American Thoracic Society
American Society of Microbiology
American Association for the Advancement of Science
American Society of Gene Therapy
Society for Biological Therapy
Year
1980
1983
1985
1987
1988
1989
1990
1991
1992
1998
1999
4
Southern Society of Clinical Investigation
American Society of Clinical Investigation
American Academy of Physicians
1999
2004
2008
---------------------------------------------------------------------------------------------------------------------HONORS
Title of Award
Year
Sigma Pi Sigma, Physics Honor Society
Ursinus College
1980
Recipient, Parker B. Francis Pulmonary
Research Fellowship Award
1991
Recipient, American Lung Association
Pediatric Pulmonary Research Fellowship
Award (declined)
1991
Recipient, Louisiana State University
Neuroscience Research Award
1991
Recipient, First Prize,
Louisiana State University
Medicine Research Day
1992
Recipient, Second Place, Merck Young
Investigator Award at the regional Southern
Society of Clinical Investigation Meeting
New Orleans, Louisiana
1993
Recipient, First Place, Merck Young
Investigator Award at the regional Southern
Society of Clinical Investigation Meeting
New Orleans, Louisiana
1994
Recipient, American Federation of Clinical
Research Young Faculty Award
1995
Recipient, American Federation of Clinical
Research Young Faculty Award
1996
Recipient, American Federation of Clinical
Research Young Faculty Award
1997
5
Inaugural Chair, Neils K. Jerne Endowed Chair
In Immunology, University of Pittsburgh
2006
---------------------------------------------------------------------------------------------------------------------PUBLICATIONS
1. Refereed articles
1. Kolls JK, Beckerman RC: Will ABPA Occur in Your Patient with Cystic Fibrosis? J
Respir Dis. 12(2):137-146, 1991.
2. Kolls JK, Kiernan MK, Ascuitto R. Intralobar Sequestration Presenting as
Congestive Heart Failure in a Neonate. Chest. 102(3):974-6, 1992
3. Greenberg S, Xie J, Wang Y, Kolls J, Shellito J, Nelson S, and Summer WR.
Ethanol relaxes pulmonary artery by release of prostaglandin and nitric oxide.
Alcohol. 10(1):21-29. 1993
4. Xie J, Wang Y, Lippton H, Cai B, Nelson S, Kolls J, Summer WR, and Greenberg
S. Tumor necrosis factor inhibits stimulated but not basal release of nitric oxide.
Amer. Rev. Resp. Dis. 148:627-636, 1993
5. Greenberg S, Xie J, Wang Y, Cai B, Kolls J, Nelson S, Hyman A, Summer WR, and
Lippton H. Tumor necrosis factor-alpha inhibits endothelial-dependent relaxation. J.
Appl. Physiol. 74:2394-2403, 1993
6. Kolls JK, Deininger P, Cohen JC, and Larson J. cDNA equalization for reversetranscription-polymerase chain reaction quantitation. Analytical Biochemistry
208(2):264-269, 1993
7. Kolls JK, Beck JM, Nelson S, Summer WR, and Shellito J. Alveolar macrophage
release of tumor necrosis factor in Pneumocystis carinii pneumonia. American
Journal of Respiratory Cell and Molecular Biology. 8(4):370-376, 1993
8. Kolls JK, Nelson S, Summer WR. Recombinant Cytokines and Pulmonary Host
Defense: A Review. American Journal of Medical Sciences. 306:330-335, 1993.
9. Xie J, Wang Y, Kolls J, Malinski T, Nelson S, Summer W, and Greenberg S. Tumor
necrosis factor- inhibits contractions to sympathetic nerve stimulation by a nitric
oxide-dependent mechanism. PSEBM. 203:446-453, 1993.
10. Midulla F, Villani A, Panuska JR, Dab I, Kolls JK, Merolla R, and Ronchetti R.
6
Respiratory syncytial virus lung infection in infants: immunoregulatory role of
infected alveolar macrophages. J. Infect. Dis. 168:1515-1519, 1993
11. Spolarics Z, Spitzer JJ, Wang JF, Xie J, Kolls J, Greenberg S. Alcohol
administration attenuates LPS-induced expression of inducible nitric oxide synthase
in kupffer and hepatic endothelial cells. Biochem. Biophys. Res. Comm. 197:606611, 1993
12. Kolls J, Peppel K, Silva M, Beutler B. Prolonged and effective blockade of TNF
activity through adenovirus-mediated gene transfer. Proc. Nat. Acad. Sci. USA,
91:215-219, 1994
13. Kolls J, Xie J, Leblanc R, Malinski T, Nelson S, Summer WR. Rapid induction of
messenger RNA for nitric oxide synthase II in rat neutrophils, in vivo, by endotoxin
and its suppression by prednisolone. Proc. Soc. Exp. Biol. Med. 205:220-229, 1994
14. Greenberg SS, Xie J, Joseph KO, Kolls J, and Summer W. In vivo administration of
endotoxin and tumor necrosis factor-alpha produce different effects on constitutive
and inducible nitric oxide synthase activity in rat neutrophils and aorta ex vivo. Proc.
Soc. Exp. Biol. Med. 208:199-208, 1995
15. Greenberg SS, Xie J, Wang Y, Kolls J, Malinski T, Summer WR, and Nelson S.
Ethanol suppresses LPS-induced mRNA for nitric oxide synthase II in alveolar
macrophages in vivo and in vitro. Alcohol 11:539-547, 1994
16. Kolls JK and Nelson S. The young adult with cystic fibrosis. Mediguide to Infectious
Disease. 13(6):1-6, 1994.
17. Kolls JK, Lei D, Nelson S, Summer WR, Greenberg S, Beutler B. Adenovirusmediated blockade of tumor necrosis factor in mice protects against endotoxic
shock yet impairs pulmonary host defense. J. Infect. Dis. 171:570-575, 1995.
18. Adams LB, Mason CM, Kolls JK, Scollard D, Krahenbuhl JL, and Nelson S.
Exacerbation of acute and chronic murine tuberculosis by administration of a TNF
receptor expressing adenovirus. J. Infect. Dis. 171:400-5, 1995.
19. Mason CM, Kolls JK, and Neslon S. Pathogenesis and host defense in pulmonary
infections. Curr.Opin.Pulm.Med. 1 (3):163-170, 1995.
20. Nelson S, Mason CM, Kolls J, and Summer WR.. Pathophysiology of pneumonia.
Clin.Chest Med. 16 (1):1-12, 1995.
21. Xie, J., Kolls, J., Bagby, G. and Greenberg, S.S. Independent suppression of nitric
oxide and TNF-alpha in the lung of conscious rats by ethanol. FASEB J. 9:253-261,
7
1995.
22. Greenberg SS, Xie J, Kolls JK, Mason C, Didier P. Rapid induction of mRNA for
nitric oxide synthase II by Mycobacterium tuberculosis and Mycobacterium avium.
Proc. Soc. Exp. Biol. Med. 209:46-53, 1995
23. Greenberg S, Xie J, Kolls J, Nelson S, Didier P, Mason C. Ethanol suppresses
Mycobacteria tuberculosis-induced mRNA for nitric oxide synthase in alveolar
macrophages, in vivo. Alcohol Clin. Exp. Res. 19:394-401, 1995.
24. Shellito JE, Kolls JK, Summer WR. Regulation of nitric oxide release by
macrophages following intratracheal lipopolysaccharide. Am. J. Resp. Cell Mol. Biol.
13:45-53, 1995
25. Kolls JK, Xie J, Lei D, Greenberg S, Summer WR, and Nelson S. Differential
effects of in vivo ethanol on LPS-induced TNF and nitric oxide production in the
lung. Am. J. Physiol. 268:L991-L998, 1995.
26. Kolls J.K., Lei D., Odom G, Nelson S, Summer WR, Gerber MA, Shellito JE. Use of
transient CD4-lymphocyte depletion to prolong transgene expression of E1-deleted
adenoviral vectors. Hum. Gene Ther. 7:489-497,1996.
27. Shellito JE, Kolls JK, Olairu R., Beck JM. Nitric oxide and host defense against
Pneumocystis carinii infection in a mouse model. J. Infect. Dis. 173:432-439, 1996
28. Lei D, Lehmann M, Shellito JE, Nelson S, Siegling A, Volk H-D, and Kolls JK. Non
depleting anti-CD4 antibody treatment prolongs lung-directed E1-deleted
adenovirus-mediated gene expression in rats. Hum. Gene Ther. 7:2273-2279, 1996
29. Kolls JK, Lei D, Vazquez C, Odom G, Summer WR, Nelson S, Shellito JE.
Exacerbation of Murine Pneumocystis Carinii Infection by Adenoviral-mediated
Gene Transfer of a TNF Inhibitor. Am. J. Resp. Cell Mol. Biol. 16:112-118, 1997.
30. Lei D, Lancaster JL, Joshi MS, Nelson S, Stoltz D, Bagby GJ, Odom G, Shellito JE,
and Kolls, JK. Activation of Alveolar Macrophages and Lung Host Defenses Using
Transfer of the Interferon-gamma Gene. 1997. Am. J. Physiol. 272:L852-L859,
1997.
31. Cheng D, Kolls JK, Lei D, and Noel RA. In vitro and In vivo Gene Transfer to Rat
Intestinal Epithelium. Hum. Gene Ther. 8:755-764, 1997
32. Sullivan DE, Dash S, Du H, Hiramatsu N, Ardin F, Kolls J, Blanchard J, Baskin G,
Gerber MA. Liver Directed Gene Transfer in Non-Human Primates. Hum. Gene
Ther. 8:1195-1206, 1997.
8
33. Kolls JK. Lei D. Nelson S. Summer WR. Shellito JE. Pulmonary cytokine gene
therapy. Adenoviral-mediated murine interferon gene transfer compartmentally
activates alveolar macrophages and enhances bacterial clearance. Chest. 111(6
Suppl):104S, 1997.
34. Schwarzenberger, P., Hunt, J.D., Robert, E., Theodossiou, C., and Kolls, J.K.
Receptor-targeted recombinant adenovirus conglomerates - a novel molecular
conjugate vector with improved expression characteristics. J. Virol. 71:8563-8571,
1997.
35. Kolls, J.K., Lei, D., Stoltz, D., Zhang, P., Schwarzenberger, P.O., Ye, P., Bagby, G.,
Summer, W.R., Shellito, J.E., and Nelson, S. Adenoviral-mediated Interferongamma Gene Therapy Augments Pulmonary Host Defense of Ethanol Treated Rats.
Alcoholism: Clin. And Exp. Res. 22:157-162, 1998.
36. Schwarzenberger, P., Harrison, L., Weinacker, A., Gaumer, R., Theodossiou C.,
Summer W., Ye, P., Marrogi, A. J., Ramesh, R., Freeman S., and Kolls, J. Gene
Therapy for Malignant Mesothelioma: A novel approach for an incurable cancer with
increased incidence in Louisiana. J. La. State Med. Soc. 150:168-174, 1998.
37. Ghivizzani, S.C., Lechman, E.R. Kang, R., Tio, C., Kolls, J., Evans, C.H. Robbins,
P.D. Direct adenovirus-mediated gene transfer of interleukin 1 and tumor necrosis
factor alpha soluble receptors to rabbit knees with experimental arthritis has local
and distal anti-arthritic effects. Proc. Nat. Acad. Sci. USA, 95(8): 4613-8, 1998.
38. Schwarzenberger, P., Lei, D., Freeman, S.M., Ye, P., Weinacker, A., Theodossiou,
C., Summer, W., and Kolls, J.K. Antitumor activity with a HSV-tk gene modified cell
line PA-1-STK in malignant mesothelioma. Am. J. Resp. Cell Mol. Biol. 19:333-337,
1998.
39. Kolls J.K. and Xie J. Measurement of TNF and iNOS mRNA using cDNAEqualized Reverse Transcriptase PCR. Methods Mol. Biol. 92:55-66, 1998.
40. Cheung AT, Ree D, Kolls JK, Fusilier J, Coy DH, and Bryer-Ash M. Evidence for
differential regulation of insulin signaling by TNF-alpha. Endocrinology
139(12):4928-35, 1998
41. Schwarzenberger P., Harrison L., Weinacker A., Marrogi A., Byrne P., Ramesh
R.,Theodossiou C., Gaumer R., Summer W., Freeman S.M., and Kolls J.K. The
treatment of malignant mesothelioma with a gene modified cancer cell line: a phase
I study. Hum. Gene Ther. 9(17):2641-9, 1998.
42. Schwarzenberger P., La Russa V., Miller A., Ye P., Huang W., Zieske A., Nelson
S., Bagby G., Stoltz D., Mynatt R., Spriggs M., and Kolls, JK. Interleukin 17
9
stimulates granulopoiesis in mice: use of an alternate, novel, gene therapy derived
method for in vivo evaluation of cytokines. J Immunol. 161(11):6383-9, 1998
43. Mason C.M., Dobard E., Kolls J., and Nelson S. Effect of alcohol on bacterial
translocation in rats. Alcohol Clin. Exp. Res. 1998 Nov;22(8):1640-5
44. Null D, Bimle C, Weisman L, Johnson K, Steichen J, Singh S, et. al. Palivizumab, a
humanized respiratory syncytial virus monoclonal antibody, reduces hospitalization
from respiratory syncytial virus infection in high-risk infants. Pediatrics 102 (3): 531537, 1998
45. Kolls J.K, Habetz S., Vazquez C., Brown J.A., Lei D., Schwarzenberger P., Ye P.,
Nelson S., and Shellito J.E. Interferon-gamma and CD8+ T-cells restore host
defenses against P. carinii in mice lacking CD4+ T-cells. J. Immunology. 162:28902894, 1999.
46. Good D., Schwarzenberger P., Eastham J., Rhoads R., Hunt J., Collins M., Batzer
M., Theodossiou C., Kolls J., and Grimes, S. Cloning and Characterization of the
Prostate Specific Membrane Antigen Promoter. Journal of Cell. Biochem. 74:395405, 1999.
47. Otterbein L.E., Kolls J.K., Mantell L.L., Cook J.L., Alam J., and A.M.K. Choi.
Exogenous administration of heme oxygenase-1 by gene transfer provides
protection against hyperoxia-induced lung injury. J. Clinical Investigation. 103:10471054, 1999.
48. Ritter T., Vogt K., Rieck P., Schilling-Schon A., Kolls J., Hartmann C., Volk H. D.,
Pleyer U. Adenovirus-mediated Gene Transfer of Interleukin-4 to Corneal
Endothelial Cells and Organ Cultured Corneas Leads to High IL-4 Expression. Exp.
Eye Res. 69:563-568, 1999.
49. Ritter T., Risch K., Schroder G., Kolls J., Siegling A., Graser E., Reinke P., Brock
J., Lehmann M., and Volk H.D. Intragraft overexpression of interleukin-4 is neither
sufficient nor essential for tolerance induction to cardiac allografts in a highresponder strain combination. Transplantation 68:1427-31, 1999.
50. Amersi F., Buelow R., Kato H., Ke B., Coito A.J., Shen X.D., Zhao D., Zaky J.,
Melinek J., Lassman C.R., Kolls J.K., Alam J., Ritter T., Volk H.D., Farmer D.G.,
Ghobrial R.M., Busuttil R.W., Kupiec-Weglinski J.W. Upregulation of heme
oxygenase-1 protects genetically fat Zucker rat livers from ischemia/reperfusion
injury. J. Clin. Invest. 104(11):1631-9,1999.
51. Schwarzenberger P., Byrne P., and Kolls J.K. Immunotherapy based treatment
strategies for malignant mesothelioma Current Opinion in Molecular Therapeutics
1:104-111, 1999
10
52. Stoltz D.A., Nelson S., Kolls J. K., Zhang P., Bohm R.P., Murphey-Corb M., Bagby
G.J. In Vitro Ethanol Suppresses Alveolar Macrophage TNF-alpha During Simian
Immunodeficiency Virus Infection. Am. J. Resp. Critical Care Med. 161(1): 1351401, 2000.
53. Mason C.M., Dobard E., Kolls J.K., Nelson S. Ethanol and Murine Interleukin (IL)12 production. Alcoholism: Clin. Exp. Res. 24:553-559, 2000.
54. Hunt, J.D., Robert, E.G., Zieske, A.W., Bautista, A.P., Bukara, M., Lei, D., Shellito,
J.E., Nelson, S., Kolls, J.K., and Skrepnik, N. Orthotopic Human Lung Cancer
Xenografts in BALB/c Mice Immunosuppressed with Anti-CD4 Monoclonal
Antibodies and Chronic Alcohol Consumption, Cancer 88:468-479 (2000).
55. Shean M.K., Baskin G., Sullivan D., Schurr J., Cavender D.E., Shellito J.E.,
Schwarzenberger P., Kolls J.K. Immunomodulation and Adenoviral Gene Transfer
to the Lungs of Non-human Primates. Human Gene Therapy. 11: 1047-1055, 2000.
56. Zhang Z., Kolls J.K., Oliver P., Good D., Schwarzenberger P.O., Joshi M., Ponthier
J.L., and J.R. Lancaster. Activation of Tumor Necrosis Factor-alpha -converting
Enzyme-mediated Ectodomain Shedding by Nitric Oxide. J. Biological Chem.
275:15839-15844, 2000
57. Schwarzenberger P., Huang W., Ye P., Oliver P., Manuel M., Zhang Z., Bagby G.,
Nelson S., and Kolls J.K. Requirement of endogenous stem cell factor and G-CSF
for IL-17 mediated granulopoiesis. J. Immunology. 164(9):4783-9, 2000.
58. Shellito J.E., Ruan S., Tate C., Kolls J.K. Murine CD4+ T-lymphocyte subsets and
host defense against Pneumocystis carinii. Journal of Infect. Dis. 181:2011-2017,
2000.
59. Cheung A.T., Wang J., Ree D., Kolls J.K., and Bryer-Ash M. Tumor necrosis
factor-induces hepatic insulin resistance in obese Zucker (fa/fa) rats via interaction
of leukocyte antigen-related tyrosine phosphatase with focal adhesion kinase.
Diabetes, 49:000-000, 2000.
60. Ritter T., Schröder G., Risch K., Vergopoulos A., Shean M.K., Kolls J., Brock, J.,
Lehmann, M., Volk, H.-D. Ischemia/Reperfusion injury mediated downregulation of
adenovirus-mediated gene expression in a rat heart transplantation is inhibited by
co-application of a TNFRp55-Ig chimeric construct. Gene Therapy, in press.
61. Rodriguez F.H., Nelson S., and Kolls J.K. Cytokine Therapeutics for Infectious
Diseases. Current Pharmaceutical Design. 6:665-680, 2000.
11
62. Zhang Z., Black R.A., Cork J., Mize R.R., Stoltz D., Ye P., Lei D., Schwarzenberger
P.O., Bagby G., Summer W.R., Shellito J.E., Nelson S., and Kolls J.K. Ethanol
Blocks TNF Production by a Post-transcriptional Mechanism. J. Leukocyte Biology,
6: 856-862, 2000.
63. Ritter,T., Schroder,G., Risch,K., Vergopoulos,A., Shean,M.K., Kolls,J., Brock,J.,
Lehmann,M., and Volk,H.D. Ischemia/reperfusion injury-mediated down-regulation
of adenovirus- mediated gene expression in a rat heart transplantation model is
inhibited by co-application of a TNFRp55-Ig chimeric construct. Gene Therapy
7:1238-1243, 2000.
64. Ritter,T., Brandt,C., Prosch,S., Vergopoulos,A., Vogt,K., Kolls,J., and Volk,H.D.
Stimulatory and inhibitory action of cytokines on the regulation of HCMV-IE promoter
activity in human endothelial cells. Cytokine 12:1163-1170, 2000.
65. Schroder,G., Risch,K., Nizze,H., Kolls,J., Reinke,P., Brock,J., Lehmann,M.,
Volk,H.D., and Ritter,T. Immune response after adenoviral gene transfer in
syngeneic heart transplants: effects of anti-CD4 monoclonal antibody therapy.
Transplantation 70:191-198, 2000.
66. Harrison LH, Schwarzenberger PO, Byrne PS, Marrogi AJ, Kolls JK, McCarthy KE.
Gene-modified PA1-STK cells home to tumor sites in patients with malignant
pleural mesothelioma. Ann. Thoracic Surgery, 70(2):407-11, 2000
67. Zhang P., Bagby G.J., Kolls J.K., Welsh D.A., Summer W.R., Andresen J., and
Nelson S. The effects of granulocyte colony-stimulating factor and neutrophil
recruitment on the pulmonary chemokine response to intratracheal endotoxin. J.
Immunology, 166(1):458-65, 2001
68. Zhang Z., Oliver P., Lancaster J.R., Schwarzenberger P.O., Joshi M.S., Cork J.,
and Kolls J.K. Reactive Oxygen Species Mediates Tumor Necrosis Factor-alpha
Converting Enzyme-Dependent Ectodomain Shedding Induced by Phorbol Myristate
Acetate. FASEB J. 15(2):303-305, 2001.
69. Stoltz D.A., Nelson S., Kolls J.K., Zhang P., Bohm R.P., Murphey-Corb M., and
Bagby G.J. Effects of simian immunodeficiency virus and alcohol on blood tumor
necrosis factor-alpha production. Journal of Leukocyte Biology (submitted), 2000.
70. Egawa K., Maegawa H., Shimizu S., Morino K., Nishio Y., Bryer-Ash M., Cheung
A.T., Kolls J.K., Kikkawa R., Kashiwagi A. Protein tyrosine phosphatase-1B
negatively regulates insulin signaling in L6 myocytes and Fao hepatoma cells. J.
Biol. Chem. 276:10207-11, 2001.
71. Schwarzenberger P, Huang W, Oliver P, Osidipe T, Theodossiou C, Kolls JK.
12
Poly-L-lysine-based molecular conjugate vectors: a high efficiency gene transfer
system for human progenitor and leukemia cells. Am. J. Med. Sci. 321(2):129-36,
2001
72. Zhong Q., Kolls J.K., and Schwarzenberger P.O. Retrovirus molecular conjugates:
A novel, high transduction efficiency, potentially safety-improved, gene transfer
system. J. Biol. Chem. 276(27):24601-7, 2001.
73. Kolls J.K., Ye .P, and Shellito J.E. Gene therapy to modify pulmonary host
defenses. Semin. Respir. Infect. 16(1):18-26, 2001
74. Lubberts E., Joosten L.A., Oppers B., van Den Bersselaar L., Coenen-De Roo C.J.,
Kolls J.K., Schwarzenberger P., van De Loo F.A., van Den Berg W.B. IL-1Independent Role of IL-17 in Synovial Inflammation and Joint Destruction During
Collagen-Induced Arthritis. J. Immunol. 167(2):1004-1013, 2001.
75. Ye P., Rodriguez F.H., Kanaly S., Stocking K.L., Schurr J., Schwarzenberger . P,
Oliver P., Huang W., Zhang P., Zhang J., Shellito J.E., Bagby G.J., Nelson S.,
Charrier K., Peschon J.J., and Kolls J.K. Requirement of IL-17 Receptor Signaling
for Lung CXC Chemokine and G-CSF Expression, Neutrophil Recruitment, and Host
Defense. J. Exp. Med., 2001 Aug 20;194(4):519-28.
76. Ye P., Garvey, P.B., Zhang P., Nelson S., Bagby G., Summer W.R.,
Schwarzenberger P., Shellito J.E., and Kolls J.K. IL-17 and lung host defense
against K. pneumoniae infection. Am. J. Respir. Cell and Mol. Biol. 25(3):335-40,
2001.
77. Shellito J.E., Zheng M., Ye, P., Ruan S., Shean M.K., and Kolls J.K. Effect of
alcohol consumption on host release of interleukin-17 during infection with Klebsiella
pneumoniae. Alcohol Clin Exp Res. 25(6):872-81, 2001.
78. Schwarzenberger P., Huang W., Oliver, P., Byrne P., LaRussa V., Zhang Z., and
Kolls J.K. Interleukin 17 Mobilizes Peripheral Blood Stem Cells with Short and
Long Term Repopulating Ability in Mice. J. Immunol., 2001 Aug 15;167(4):2081-6.
79. Zhong Q., Oliver P., Huang W., Good D., La Russa V., Zhang Z., Cork J.R., Veith
R.W., Theodossiou C., Kolls J.K., and Schwarzenberger P. Efficient c-kit Receptor
Targeted Gene Transfer to Primary, Human CD34 Selected Hematopoietic Stem
Cells. J Virol. 2001 Nov;75(21):10393-400.
80. Forlow, S.B. , Schurr, J.R., Kolls, J.K., Bagby, G.J., Schwarzenberger, P.O., and K.
Ley. Increased Granulopoiesis through IL-17 and G-CSF in Leukocyte Adhesion
Molecule-Deficient Mice. Blood. 98(12):3309-14, 2001.
13
81. Zhang Z, Bagby GJ, Stoltz D, Oliver P, Schwarzenberger PO, Kolls JK. Prolonged
ethanol treatment enhances lipopolysaccharide/phorbol myristate acetate-induced
tumor necrosis factor-alpha production in human monocytic cells. Alcohol Clin Exp
Res. 2001 Mar;25(3):444-9.
82. Zheng M., Shellito J.E., Marrero L., Zhong Q., Julian S., Ye P., Wallace V.,
Schwarzenberger P., and Kolls, J.K. CD4+ T-cell independent vaccination against
Pneumocystis carinii in mice. J. Clin. Investigation, 108(10): 1469-74, 2001.
83. Weiss DJ, Baskin GB, Shean MK, Blanchard JL, and Kolls JK. Use of perflubron to
enhance lung gene expression: safety and initial efficacy studies in non-human
primates. Mol. Ther. 5(1):8-15, 2002.
84. Schwarzenberger P. and Kolls J.K. Interleukin 17: an example for gene therapy as
a tool to study cytokine mediated regulation of hematopoiesis. J. Cellular
Biochemistry, 2002 (in press).
85. Nelson S. and Kolls J.K. Alcohol, host defence and society. Nature Reviews
Immunology, 2:205-209, 2002
86. Zhang P, Bagby GJ, Boe DM, Zhong Q, Schwarzenberger P, Kolls JK, Summer
WR, Nelson S. Acute alcohol intoxication suppresses the CXC chemokine
response during endotoxemia. Alcohol Clin Exp Res. 2002 Jan;26(1):65-73.
87. Byrne P, Huang W, Wallace VM, Shean MK, Zhang Z, Zhong Q, Theodossiou C,
Blakesley H, Kolls JK, Schwarzenberger P. Chimerism analysis in sex-mismatched
murine transplantation using quantitative real-time PCR. Biotechniques. 2002
Feb;32(2):279-80, 282-4, 286.
88. La Russa VF, Schwarzenberger P, Miller A, Agrawal K, Kolls J, Weiner R. Marrow
stem cells, mesenchymal progenitor cells, and stromal progeny. Cancer Invest.
2002;20(1):110-23.
89. Schwarzenberger P, Kolls JK, La Russa V. Hematopoietic stem cells. Cancer
Invest. 2002;20(1):124-38.
90. Prakash O, Tang ZY, Zhou P, Peng X, Kolls J, Shellito JE, Nelson S. Ethanol
decreases the efficiency of phosphorylation of thymidine kinase in a human Tlymphocytic cell line. Alcohol Clin. Exp. Res. 2002 Mar;26(3):295-302.
91. Lubberts E, Joosten LA, van de Loo FA, Schwarzenberger P, Kolls J, van den
Berg WB. Overexpression of IL-17 in the knee joint of collagen type II immunized
mice promotes collagen arthritis and aggravates joint destruction. Inflamm. Res.
2002 Feb;51(2):102-4.
14
92. Stoltz DA, Nelson S, Kolls JK, Zhang P, Bohm RP, Murphey-Corb M, Bagby GJ.
Effects of in vitro ethanol on tumor necrosis factor-alpha production by blood
obtained from simian immunodeficiency virus-infected rhesus macaques. Alcohol
Clin Exp Res. 2002 Apr;26(4):527-34.
93. Zhang XY, La Russa VF, Bao L, Kolls J, Schwarzenberger P, Reiser J. Lentiviral
vectors for sustained transgene expression in human bone marrow-derived stromal
cells. Mol Ther. 2002 May;5(5 Pt 1):555-65.
94. Quinton LJ, Nelson S, Boe DM, Zhang P, Zhong Q, Kolls JK, Bagby GJ. The
granulocyte colony-stimulating factor response after intrapulmonary and systemic
bacterial challenges. J Infect Dis. 2002 May 15;185(10):1476-82.
95. Spitzer JA, Zheng M, Kolls JK, Stouwe CV, Spitzer JJ. Ethanol and LPS modulate
NF-kappaB activation, inducible no synthase and cox-2 gene expression in rat liver
cells in vivo. Front Biosci. 2002 May 1;7:A99-A108.
96. Steele C., Zheng M., Young E., Marrero L., Shellito J.E., and Kolls J.K. Increased
Host Resistance Against Pneumocystis carinii Pneumonia in  T Cell-Deficient
Mice: Protective Role of IFN-g and CD8+ T Cells. Infect. Immun. 70(9):5208-15,
2002.
97. Schwarzenberger P. and Kolls J.K. Interleukin 17: an example for gene therapy as
a tool to study cytokine mediated regulation of hematopoiesis. J Cell Biochem
Suppl. 2002;Suppl 38:88-95.
98. Ruan S, Tate C, Lee JJ, Ritter T, Kolls JK, Shellito JE. Local delivery of the viral
IL-10 gene suppresses tissue inflammation in murine P. carinii infection. Infection
and Immunity, 70(11):6107-6113, 2002.
99. Zhao XJ, Marrero L, Song K, Oliver P, Chin SY, Simon H, Schurr JR, Zhang Z,
Thoppil D, Lee S, Nelson S, Kolls JK.. Acute Alcohol Inhibits TNF-alpha Processing
in Human Monocytes by Inhibiting TNF/TNF-alpha-Converting Enzyme Interactions
in the Cell Membrane. J Immunol. 2003 Mar 15;170(6):2923-31.
100. Bagby GJ., Stoltz DA,, Zhang P, Kolls JK, Brown J, Bohm RP, Rockar R,
Purcell J, Murphey-Corb M, and Nelson S. The Effect of Chronic Binge Ethanol
Consumption on the Primary Stage of SIV Infection in Rhesus Macaque. Alcohol
Clin Exp Res 2003 Mar;27(3):495-502
101. Lalonde ES, Beyer G, Friedlander PL, Kolls JK. Efficacy of transfection rates
on head and neck squamous cell cancer by a novel adenovirus: An in vivo and in
vitro study. Head Neck 24(12):1038-46, 2002
15
102. Kolls JK, Kanaly ST, Ramsay AJ. Interleukin 17: an emerging role in lung
Inflammation. Am J Respir Cell Mol Biol 2003 Jan;28(1):9-11
103. Happel K, Zheng M, Schurr JR, Shellito J, Bagby G, Nelson S, and Kolls JK.
Cutting Edge: Role of TLR4 signaling and IL-23 in IL-17 elaboration in response to
Klebsiella pneumoniae infection. J Immunol. 2003 May 1;170(9):4432-6.
104. Collier JJ, Doan TT, Daniels MC, Schurr JR, Kolls JK, Scott DK. J Biol Chem.
2003 Feb 21;278(8):6588-95.
105. Yang J, Reutzel-Selke A, Steier C, Jurisch A, Tullius SG, Sawitzki B, Kolls J,
Volk HD, Ritter T. Targeting of Macrophage Activity by Adenovirus-Mediated
Intragraft Overexpression of TNFRp55-Ig, IL-12p40, and vIL-10 Ameliorates
Adenovirus-Mediated Chronic Graft Injury, whereas Stimulation of Macrophages by
Overexpression of IFN-gamma Accelerates Chronic Graft Injury in a Rat Renal
Allograft Model. J Am Soc Nephrol. 2003 Jan;14(1):214-25.
106. Zhong Q, Kolls JK, Schwarzenberger P. Retrovirus molecular conjugates. Cell
Mol Life Sci. 2002 Dec;59(12):2083-7.
107. Lubberts E, Van Den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P,
Coenen-De Roo CJ, Kolls JK, Joosten LA, Van Den Berg WB. IL-17 Promotes
Bone Erosion in Murine Collagen-Induced Arthritis Through Loss of the Receptor
Activator of NF-kappaB Ligand/Osteoprotegerin Balance. J Immunol 2003 Mar
1;170(5):2655-62.
108. Silverstein DM, Travis BR, Thornhill BA, Schurr JS, Kolls JK, Leung JC,
Chevalier RL. Altered expression of immune modulator and structural genes in
neonatal unilateral ureteral obstruction. Kidney Int. 2003 Jul;64(1):25-35.
109. Friedlander PL, Delaune CL, Abadie JM, Toups M, LaCour J, Marrero L, Zhong
Q, Kolls JK. Efficacy of CD40 Ligand Gene Therapy in Malignant Mesothelioma.
Am J Respir Cell Mol Biol. 2003 Apr 3
110. Nabbe KC, van Lent PL, Holthuysen AE, Kolls JK, Verbeek S, van den Berg WB.
FcgammaRI up-regulation induced by local adenoviral-mediated interferon-gamma
production aggravates chondrocyte death during immune complex-mediated
arthritis. Am J Pathol. 2003 Aug;163(2):743-52.
111. Lanson NA, Friedlander PL, Schwarzenberger P, Kolls JK, and Wang G.
Replication of an Adenoviral Vector Controlled by the Human Telomerase Reverse
Transcriptase Promoter Causes Tumor-Selective Tumor Lysis. Cancer
63(22):7936-7941, 2003.
16
112. Steele C, Marrero L, Swain S, Harmsen AG, Zheng M, Brown GD, Gordon S,
Shellito JE, and Kolls JK. Alveolar macrophage-mediated killing of Pneumocystis
carinii f. sp. muris involves molecular recognition by the Dectin-1 -glucan receptor.
J. Exp. Med. 198(11):1677-1688, 2003.
113. Weiss DJ, Beckett T, Bonneau L, Young J, Kolls JK, Wang G. Transient
increase in lung epithelial tight junction permeability: an additional mechanism for
enhancement of lung transgene expression by perfluorochemical liquids. Mol Ther.
8(6):927-35, 2003
114. McAllister F, Steele C, Zheng M, Young M, Shellito JE, Marrero L, Kolls JK. Tc1
CD8+ T-cells are effector cells against Pneumocystis in mice. J. Immunology.
172(2):1132-8, 2004.
115. Lam NT, Lewis JT, Cheung AT, Luk CT, Tse J, Wang J, Bryer-Ash M, Kolls JK,
Kieffer TJ. Leptin Increases Hepatic Insulin Sensitivity and Protein Tyrosine
Phosphatase 1B Expression. Molecular Endocrinology 2004
116. Kolls JK. HIV Immunology and new therapies. Pediatr Pulmonol. 2004 Feb;37
Suppl 26:59-61.
117. Dubin PJ, Kolls JK. Further defining lung SP cells: their origin and their
heterogeneity, now if we only knew their fate! Am J Physiol Lung Cell Mol Physiol.
2004 Sep;287(3):L475-6.
118. Zhao XJ, Oliver P, Song K, Schurr J, Zhang Z, Kolls JK. Chronic Ethanol
Enhances Ectodomain Shedding in T Cells and Monocytes. Alcohol Clin Exp Res.
2004 Sep;28(9):1399-1407.
119. Kolls JK and Linden A. IL-17 family members and Inflammation. Immunity.
2004 Oct;21(4):467-76.
120. Wang G, Bunnell BA, Painter RG, Quiniones BC, Tom S, Lanson NA Jr, Spees
JL, Bertucci D, Peister A, Weiss DJ, Valentine VG, Prockop DJ, Kolls JK. Adult
stem cells from bone marrow stroma differentiate into airway epithelial cells:
Potential therapy for cystic fibrosis. Proc Natl Acad Sci U S A. 102:186-91, 2005
121. Schurr JR, Young E, Byrne P, Steele C, Shellito JE, Kolls JK. Central role of
toll-like receptor 4 signaling and host defense in experimental pneumonia caused by
gram-negative bacteria. Infect Immun. 2005 Jan;73(1):532-45.
122. Kelly MN, Kolls JK, Happel K, Schwartzman JD, Schwarzenberger P, Combe C,
Moretto M, Khan IA. Interleukin-17/Interleukin-17 Receptor-Mediated Signaling Is
Important for Generation of an Optimal Polymorphonuclear Response against
17
Toxoplasma gondii Infection. Infect Immun. 2005 Jan;73(1):617-21.
123. Kolls JK, Zhang Z. Anti-interleukin-12 antibody for active Crohn's disease. N
Engl J Med. 10: 352(6):627-8, 2005.
124. Steele C, Shellito JE, Kolls JK. Immunity against the opportunistic fungal
pathogen Pneumocystis. Med Mycol. ;43(1):1-19, 2005
125. Koenders MI, Lubberts E, Oppers-Walgreen B, van den Bersselaar L, Helsen
MM, Kolls JK, Joosten LA, van den Berg WB. Induction of cartilage damage by
overexpression of T cell interleukin-17A in experimental arthritis in mice deficient in
interleukin-1. Arthritis Rheum. 52(3):975-83, 2005
126. Nabbe KC, Boross P, Holthuysen AE, Sloetjes AW, Kolls JK, Verbeek S, van
Lent PL, van Den Berg WB. Joint inflammation and chondrocyte death become
independent of Fcgamma receptor type III by local overexpression of interferongamma during immune complex-mediated arthritis. Arthritis Rheum. 52(3):967-74,
2005
127. Mc Allister F, Henry A, Kreindler JL, Dubin PJ, Ulrich L, Steele C, Finder JD,
Pilewski JM , Carreno B, Goldman SJ , Pirhonen J, and Kolls JK. Role of IL-17A,
IL-17F and the IL-17 receptor in regulating Gro-alpha and G-CSF in Bronchial
Epithelium: implications for airway inflammation in cystic fibrosis. J Immunol
175(1):404-12, 2005.
128. Lubberts E, Schwarzenberger P, Huang W, Schurr JR, Peschon JJ, van den
Berg WB, and Kolls JK. Requirement of IL-17 receptor signaling in radiation
resistant cells in the joint for full progression of destructive synovitis. J Immunol
175(5):3360-8, 2005.
129. Koenders M, Kolls JK, Oppers-Walgreen B, van den Bersselaar L, Joosten
LAB, Schurr JR, Schwarzenberger P, van den Berg WB, and Lubberts E. IL-17
receptor deficiency results in impaired synovial expression of IL-1 and MMP-3,-9,-13
and prevents cartilage destruction during chronic reactivated streptococcal cell wallinduced arthritis. Arthritis and Rheum. 52(10):3239-47, 2005.
130. van Lent PL, Nabbe KC, Blom AB, Sloetjes A, Holthuysen AE, Kolls J, Van De
Loo FA, Holland SM, Van Den Berg WB. NADPH-oxidase-driven oxygen radical
production determines chondrocyte death and partly regulates metalloproteinasemediated cartilage matrix degradation during interferon-gamma-stimulated immune
complex arthritis. Arthritis Res Ther. 2005;7(4):R885-95.
131. Hashimoto K, Durbin J, Zhou W, Collins RD, Ho SB, Kolls JK, Dubin PJ, Sheller
JR, Goleniewska K, O’Neal JF, Olson SJ, Mitchell D, Graham BS, Peebles RS.
Respiratory syncytial virus infection in the absence of signal transducer and activator
18
of transcription 1 results in airway dysfunction, airway mucus, and augmented IL-17
levels. JACI, 116(3):550-7, 2005.
132. Happel K, Dubin PJ, Zheng M, Ghilardi N, Lockhart C, Quinton L, Odden AR,
Shellito JE, Bagby GJ, Nelson S, and Kolls JK. Divergent roles of IL-23 and IL-12
in Host Defense against Klebsiella Pneumoniae. J. Exp. Med. 202(6):761-9, 2005.
133. Ruan S, Young E, Luce MJ, Reiser J, Kolls JK, Shellito JE. Conditional
expression of interferon-gamma to enhance host responses to pulmonary bacterial
infection. Pulm Pharmacol Ther. 2005.
134. Pop SM, Kolls JK, Steele C. Pneumocystis: Immune recognition and evasion.
Int J Biochem Cell Biol. 2005.
135. Koenders MI, Kolls JK, Oppers-Walgreen B, Van Den Bersselaar L, Joosten LA,
Schurr JR, Schwarzenberger P, Van Den Berg WB, Lubberts E. Interleukin-17
receptor deficiency results in impaired synovial expression of interleukin-1 and
matrix metalloproteinases 3, 9, and 13 and prevents cartilage destruction during
chronic reactivated streptococcal cell wall-induced arthritis. Arthritis Rheum.
52(10):3239-3247, 2005.
136. Thiele GM, Mandrekar P, Zakhari S, Hoek J, Cook RT, Ray NB, Happel KI, Kolls
JK, Kovacs EJ, Szab G. RSA 2004: Combined Basic Research Satellite
Symposium-Mechanisms of Alcohol-Mediated Organ and Tissue Damage:
Inflammation and Immunity and Alcohol and Mitochondrial Metabolism: At the
Crossroads of Life and Death Session One: Alcohol, Cellular and Organ Damage.
Alcohol Clin Exp Res. 29(9):1735-1743, 2005
137. McAllister F, Steele C, Zheng M, Shellito JE, and Kolls JK. In Vitro Effector
Activity of Pneumocystis murina-Specific T-Cytotoxic-1 CD8+ T Cells: Role of
Granulocyte-Macrophage Colony-Stimulating Factor. Infect Immun. 73(11):7450-7,
2005.
138. Steele C, Rapaka RR, Metz A, Pop SM, Williams DL, Gordon S, Kolls JK,
Brown GD. The Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies
of Aspergillus fumigatus. PLoS Pathog. 2005 Dec 9;1(4):e42
139. Song K, Zhao XJ, Marrero L, Oliver P, Nelson S, Kolls JK. Alcohol reversibly
disrupts TNF-alpha/TACE interactions in the cell membrane. Respir Res. 2005 Oct
24;6:123.
140. Zheng M, Ramsay AJ, Robichaux MB, Norris KA, Kliment C, Crowe C, Rapaka
RR, Steele C, McAllister F, Shellito JE, Marrero L, Schwarzenberger P, Zhong Q,
Kolls JK. CD4+ T cell-independent DNA vaccination against opportunistic
19
infections. J Clin Invest. 115(12):3536-44, 2005.
141. Hallows KR, Fitch AC, Richardson CA, Reynolds PR, Clancy JP, Dagher PC,
Witters LA, Kolls JK, Pilewski JM. Upregulation of AMP-activated kinase by
dysfunctional CFTR in cystic fibrosis airway epithelial cells mitigates excessive
inflammation. J Biol Chem. 281(7):4231-41, 2006.
142. Weiss DJ, Berberich MA, Borok Z, Gail DB, Kolls JK, Penland C, Prockop DJ.
Adult stem cells, lung biology, and lung disease. Proc Am Thorac Soc. (3):193-207,
2006.
143. Koenders MI, Lubberts E, van de Loo FA, Oppers-Walgreen B, van den
Bersselaar L, Helsen MM, Kolls JK, Di Padova FE, Joosten LA, van den Berg WB.
Interleukin-17 acts independently of TNF-alpha under arthritic conditions. J
Immunol. 176(10):6262-9, 2006.
144. Zhang Z, Zheng M, Bindas J, Schwarzenberger P, Kolls JK. Critical role of IL17 receptor signaling in acute TNBS-induced colitis. Inflamm Bowel Dis. 12(5):3828, 2006.
145. McAllister F., Ruan S., Steele C., Zheng M., McKinley L., Ulrich L., Marrero L.,
Shellito J.E., and Kolls JK. CXCR3 and IP-10 in Pneumocystis pneumonia. J.
Immunol. 177(3):1846-54, 2006.
146. Brown LA, Cook RT, Jerrells TR, Kolls JK, Nagy LE, Szabo G, Wands JR,
Kovacs EJ. Acute and chronic alcohol abuse modulates immunity. Alcohol Clin Exp
Res. 30(9):1624-31, 2006.
147. McKinley L, Logar AJ, McAllister F, Zheng M, Steele C, and Kolls JK.
Regulatory T Cells Dampen Pulmonary Inflammation and Lung Injury in an Animal
Model of Pneumocystis Pneumonia. J. Immunol. 177(9):6215-6226, 2006.
148. Dubin PJ, and Kolls JK. IL-23 mediates inflammatory responses to mucoid
Pseudomonas aeruginosa lung infection in mice. Am J Physiol Lung Cell Mol
Physiol. 292(2):L519-28, 2007.
149. Ivanov S, Bozinovski S, Bossios A, Valadi H, Vlahos R, Malmhall C, Sjostrand M,
Kolls JK, Anderson GP, Linden A. Functional Relevance of the IL-23-IL-17 Axis in
Lungs in vivo. Am J Respir Cell Mol Biol. 36(4):442-451. 2007.
150. Rapaka RR, Goetzman ES, Zheng M, Vockley J, McKinley L, Kolls JK, Steele
C. Enhanced defense against Pneumocystis carinii mediated by a novel dectin-1
receptor Fc fusion protein. J Immunol. 178(6):3702-12, 2007.
20
151. Zhang Z, Hinrichs DJ, Lu H, Chen H, Zhong W, Kolls JK. After interleukin12p40, are interleukin-23 and interleukin-17 the next therapeutic targets for
inflammatory bowel disease? Int Immunopharmacol. 7(4):409-16, 2007.
152. Kryczek I, Wei S, Zou L, Altuwaijri S, Szeliga W, Kolls J, Chang A, Zou W.
Cutting Edge: Th17 and regulatory T cell dynamics and the regulation by IL-2 in the
tumor microenvironment. J Immunol. 2007 Jun 1;178(11):6730-3.
153. Kuestner RE, Taft DW, Haran A, Brandt CS, Brender T, Lum K, Harder B,
Okada S, Ostrander CD, Kreindler JL, Aujla SJ, Reardon B, Moore M, Shea P,
Schreckhise R, Bukowski TR, Presnell S, Guerra-Lewis P, Parrish-Novak J,
Ellsworth JL, Jaspers S, Lewis KE, Appleby M, Kolls JK, Rixon M, West JW, Gao
Z, Levin SD. Identification of the IL-17 receptor related molecule IL-17RC as the
receptor for IL-17F. J Immunol. 2007 Oct 15;179(8):5462-73.
154. Hsu HC, Yang P, Wang J, Wu Q, Myers R, Chen J, Yi J, Guentert T, Tousson A,
Stanus AL, Le TV, Lorenz RG, Xu H, Kolls JK, Carter RH, Chaplin DD, Williams
RW, Mountz JD. Interleukin 17-producing T helper cells and interleukin 17
orchestrate autoreactive germinal center development in autoimmune BXD2 mice.
Nat Immunol. Nat Immunol. 2008 Feb;9(2):166-75
155. Aujla S, Chan YC, Zheng M, Fei M, Askew DJ, Pociask DA , Reinhart TA,
McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM,
Myerburg MM, Mason CA, Iwakura Y, and Kolls JK.
IL-22 mediates mucosal
host defense against gram negative bacterial pneumonia. Nat Med. 2008
Mar;14(3):275-81.
156. Raffatellu M, Santos RL, Verhoeven D, Wilson RP, Winter SE, Godinez I,
Sankaran S, Paixao T, George MD, Gordon MA, Kolls JK, Dandekar S, and
Bäumler AJ. IL-17 orchestrates a mucosal response against Salmonella
dissemination from the gut.. Nat Med. 2008 Apr;14(4):421-8.
157. Ruan S, McKinley L, Zheng M, Rudner X, D'Souza A, Kolls JK, Shellito JE.
Interleukin-12 and Host Defense against Murine Pneumocystis Pneumonia. Infect
Immun. 2008 Mar 10;
158. Ouyang W, Kolls JK, Zheng Y. The biological functions of T helper 17 cell
effector cytokines in inflammation. Immunity. 2008 Apr;28(4):454-67.
159. Weiss DJ, Kolls JK, Ortiz LA, Panoskaltsis-Mortari A, Prockop DJ. Stem cells
and cell therapies in lung biology and lung diseases. Proc Am Thorac Soc. 2008
Jul;5(5):637-67
160.
Rapaka RR, Kolls JK. Pathogenesis of allergic bronchopulmonary aspergillosis
21
in cystic fibrosis: current understanding and future directions. Med Mycol. 2008 Jul
30:1-7.
161. Zhao XJ, Dong Q, Bindas J, Piganelli JD, Magill A, Reiser J, Kolls JK. TRIF and
IRF-3 binding to the TNF promoter results in macrophage TNF dysregulation and
steatosis induced by chronic ethanol. J Immunol. 2008 Sep 1;181(5):3049-56.
162. Lu YJ, Gross J, Bogaert D, Finn A, Bagrade L, Zhang Q, Kolls JK, Srivastava A,
Lundgren A, Forte S, Thompson CM, Harney KF, Anderson PW, Lipsitch M, Malley
R. Interleukin-17A mediates acquired immunity to pneumococcal colonization. PLoS
Pathog. 2008 Sep 19;4(9):e1000159.
163. McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A,
Irvin CG, Piganelli JD, Ray A, Kolls JK. TH17 cells mediate steroid-resistant airway
inflammation and airway hyperresponsiveness in mice. J Immunol. 2008 Sep
15;181(6):4089-97.
164. Kolls JK, McCray PB Jr, Chan YR. Cytokine-mediated regulation of antimicrobial
proteins. Nat Rev Immunol. 2008 Nov;8(11):829-35.
165. Nagata T, McKinley L, Peschon JJ, Alcorn JF, Aujla SJ, Kolls JK. Requirement
of IL-17RA in Con A induced hepatitis and negative regulation of IL-17 production in
mouse T cells. J Immunol. 2008 Dec 1;181(11):7473-9.
166. Kreindler JL, Bertrand CA, Lee RJ, Karasic T, Aujla S, Pilewski JM, Frizzell RA,
Kolls JK. Interleukin-17A induces bicarbonate secretion in normal human bronchial
epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2008 Dec 12.
167. Sieve AN, Meeks KD, Bodhankar S, Lee S, Kolls JK, Simecka JW, Berg RE.
A novel IL-17-dependent mechanism of cross protection: respiratory infection with
mycoplasma protects against a secondary listeria infection. Eur J Immunol. 2009
Feb;39(2):426-38.
2. Reviews, invited published papers, proceedings of conference and symposia,
monographs, books and book chapters.
Reviews
1. Steele RW, Thomas M., and Kolls JK. Current Management of CommunityAcquired Pneumonia in Children: An Algorithmic Guideline Recommendation.
22
Infect. Med. 16(1):46-54, 1999
2. Rodriguez FH, Nelson S, Kolls JK. Cytokine therapeutics for infectious diseases.
Curr. Pharm. Des. 6(6):665-80, 2000.
3. Kolls J.K., Ye P., Shellito J.E. Gene Therapy to Modify Pulmonary Host Defenses.
Semin. Respir. Infect. 16(1):18-26, 2001.
4. Kolls, J.K. and Nelson, S. "Alcohol, Immunology, and Society" Nature Reviews
Immunology, 2002.
5. Khan S, Kolls JK. Malnutrition in the critically ill: don't hold the leptin. Am J Respir
Crit Care Med. 173(2):140-1, 2006.
6. Kolls JK. Oxidative stress in sepsis: a redox redux. J Clin Invest. 116(4):860-3,
2006.
7. Flotte TR, Ng P, Dylla DE, McCray PB Jr, Wang G, Kolls JK, Hu J. Viral vectormediated and cell-based therapies for treatment of cystic fibrosis. Mol Ther. 2007
Feb;15(2):229-41.
8. Dubin PJ, McAllister F, Kolls JK. Is cystic fibrosis a TH17 disease? Inflamm Res.
2007 Jun;56(6):221-7.
9. Kolls JK. Balancing mucosal immunity: caught between CYLD and Charybdis.
Immunity. 2007 Aug;27(2):187-9.
10. Zhong W, Kolls JK, Chen H, McAllister F, Oliver PD, Zhang Z. Chemokines
orchestrate leukocyte trafficking in inflammatory bowel disease. Front Biosci. 2008
Jan 1;13:1654-64.
11. Aujla SJ, Dubin PJ, Kolls JK. Th17 cells and mucosal host defense. Semin
Immunol. 2007 Nov 26;
12. Aujla SJ, Dubin PJ, Kolls JK. Interleukin-17 in pulmonary host defense. Exp Lung
Res. 2007 Dec;33(10):507-18.
13. Aujla SJ, Kolls JK. IL-22: A critical mediator in mucosal host defense. J Mol Med.
2009 Feb 14.
14. Dubin PJ, Kolls JK. Th17 cytokines and mucosal immunity. Immunol Rev. 2008
Dec;226:160-71.
15. Dubin PJ, Kolls JK. Interleukin-17A and interleukin-17F: a tale of two cytokines.
Immunity. 2009 Jan;30(1):9-11
23
Book Chapters
1. Kolls, J.K. and Shellito J. Immunology of Pneumocystis carinii Pneumonia. in
Immunopathology of Inflammatory Lung Disaese. R. Kradin and B. Robinson eds.
Butterworth/Heinmann. Stoneham, MA. 1996, pp 325-338.
2. Kolls J.K., Mason C., and Nelson, S. The effect of ethanol on tumor necrosis factor
and cytokine production in lung antibacterial defenses. in Drugs of Abuse, Immunity,
and Infections. H. Friedman, T. Klein, and S. Specter eds. CRC Press. Boca Raton, FL
1996, pp. 143-155
3. Kolls J.K. and Xie J. Measurement of TNF and iNOS mRNA using cDNA-Equalized
Reverse Transcriptase PCR. In Methods In Molecular Biology: PCR in Bioanalysis.
Stephen J. Meltzer ed. Humana Press, Clifton, NJ. 92:55-66, 1998.
4. Kolls J.K., Rhoades B., and Standiford T. "Gene Therapy, Cytokines and Host
Defense" in Cytokines in Pulmonary Disease: Infection and Inflammation Marcel
Dekker, New York, N.Y. 2000.
5. Kolls J.K. Gene Therapy for Pulmonary Infections in “Science and Medicine”.
Joanne T. Douglas and David T. Curiel, Editors. Science and Medicine, Inc.
Philadelphia, PA. 5:4-5, 1998.
6. Kolls J.K. "Gene Therapy for HIV and Aids-related opportunistic infections" in Gene
Therapy in the ICU, editor Dr. Phillip Factor. Kluwer Academic Publishers, Boston, p.
147-1558, 2001.
7. Prellop P. and Kolls J.K. "Use of Gene Therapy to Study Lung Infection and
Immunity" in Gene Therapy in Lung Disease, Steven Albelda, M.D., Editor, Marcel
Dekker, New York, N.Y. , 200.21 (in press).
3. Published Abstracts
1. Kolls JK, Karam GH: Epidemiology of Congenital Cytomegalovirus Infection
(abstract). Presented at the National American College of Physicians Meeting; San
Francisco, CA April, 1989.
2. Kolls JK, Kiernan MK, Ascuitto R. Intralobar Sequestration Presenting as
Congestive Heart Failure in a Neonate (abstract). Presented at the 1990 American
College of Chest Physicians Meeting. Toronto, Canada, October 1990.
24
3. Kolls JK, Nelson S, Beckerman RC, Summer WR: Cell Populations in
bronchoalveolar lavage in severe respiratory syncytial virus infection (abstract).
Presented at the 1991 American College of Chest Physicians Meeting, San Francisco,
California.
4. Kolls JK, Larson J, Deininger P, Shellito J, Nelson S, Beckerman RC, Summer
WR: Constitutive Expression of mRNA For Tumor Necrosis Factor Alpha in Murine
Alveolar Macrophages. Am. Rev. Resp. Dis.145:a833, 1992
5. Kolls JK, Xie J, Werner A, LeBlanc R, Shellito J, Malinski T, Summer WR, and
Greenberg S. Rapid induction of mRNA for nitric oxide synthase II by endotoxin and
inhibition of its transcription by prednisolone. (abstract) Clin Res 40:827A, 1992
6. Kolls J, Peppel K, Silva M, and Beutler. Prolonged and effective blockade of TNF
activity (in vivo) through inhibitor gene transfer: a versatile alternative to gene knockout.
American Society of Hematology Meeting. December 1993 and February 1994,
Southern Society of Clinical Investigation, New Orleans, LA2.
7. Kolls JK, Lyons CR, Lovchik J, Silva M, Lipscomb M, and Beutler B. Modulation of
the acute pulmonary inflammatory response by adenovirus-mediated gene transfer.
Amer. Rev. Resp. Dis. 1994
8. Kolls JK, Mason C, Adams L, Shellito JE, Lei D, and Nelson S. Influence of
adenoviral mediated gene transfer of a TNF soluble recptor on pulmonary host
defenses. Accepted for presentation at the 1995 Southern Society of Clinical
Investigation Meeting. New Orleans, LA.
9. Lei D, Nelson S, Xie J, Greenberg S, and Kolls JK. Divergent effects of in vivo
ethanol on pulmonary TNF and inducible nitric oxide synthase gene expression.
Accepted for presentation at the 1995 Southern Society of Clinical Investigation
Meeting. New Orleans, LA.
10. Odom G, Lei D, Shellito JE, Kolls JK. Pre-treatment with a depleting monoclonal
anti-CD4 antibody prolongs in vivo transgene expression of a recombinant adenoviral
vector. Accepted for presentation at the 1995 Southern Society of Clinical Investigation
Meeting. New Orleans, LA.
11. Kolls JK, Allen K, Lei D, Weinacker A, Freeman S, Summer WR. Comparative
efficacy of killing a mesothelioma cell line by delivery of the HSVtk gene with a
permanently transduced cell line versus an adenoviral vector. J. Invest. Med. 44:62A,
1996
12. Kolls JK. Pulmonary Cytokine Gene Therapy. Presented at the 1996 Aspen Lung
Conference. Aspen, CO.
25
16. Other Publications
Commentaries
1. Khan S, Kolls JK. Malnutrition in the Critically III: Don't Hold the Leptin.
Am J Respir Crit Care Med. 2006 Jan 15;173(2):140-1.
-------------------------------------------------------------------------------------------------------------------PROFESSIONAL ACTIVITIES
TEACHING:
LSUHSC - New Orleans
Lectures on “Pulmonary Potpourri” and “Pediatric Pneumonia” given on a
quarterly basis to LSU Third-Year Students during the Pediatric Block.
Lectures on “Acute Respiratory Failure” and “Pneumonia” given to
Pediatric Residents on an annual basis.
Ward Attending on the Pulmonary Service at Children’s Hospital 8
weeks/year
Attending, University Hospital Pediatric Pulmonary Consultations 52
weeks/year
Director, Pediatric Pulmonary Lab at Medical Center of Louisiana and
University Hospital
Instructor, Introduction to Clinical Medicine since 1993.
Lecture on “Acute Respiratory Failure” and "Respiratory Infections"
LSU pediatric Board Review, annually 1994-2002
University of Pittsburgh
MSTP Molecular Medicine Course – Gene Therapy and
Insertional Mutagenesis
Department of Immunology Seminar Series: IL-17 and Lung
Inflammation
Mentor, MSTP Journal Club
26
First Year Medicine PBL in Pediatrics
Children’s Hospital of Pittsburgh
Epidemic Asthma and Pneumonia Lectures to Housestaff
and Fellows
Thesis Committees:
1995-1996, Served on Thesis Committee, Debbie Sullivan,
Tulane Dept. of Pathology.
1998-2001, Served on Thesis Committee, Chad Steele, LSU
Dept. of Microbiology, Immunology, and Parasitology.
1998-2001, Served on Thesis Committee, Julie Ponthier,
LSU Dept. of Physiology.
1999-2001, Served on Thesis Committee, David Colter,
Tulane Dept. of Biochemistry
1998-2001, Served on Thesis Committee, Floyd Wormley,
LSU Dept. of Microbiology, Immunology, and Parasitology
2000-2003, Served on Thesis Committee, Lee Quinton, LSU
Dept. of Physiology.
2004-2006, Served on Thesis Committee, Russ Traister,
University of Pittsburgh Dept. of Immunology
Training of Students and Post-doctoral Fellows:
LSUHSC - New Orleans
2001-2003
Mentor, Dr. Kyle Happel, Pulmonary Fellow, LSUHSC, New
Orleans
2001-2003
Mentor, Dr. Chad Steele, Post-Doctoral Fellow, LSUHSC
New Orleans
2001-2003
Mentor, Ms. Natasha Brown, Recipient, NHLBI Minority High
School Supplement
2001-2003
Mentor, Mr. Stewart Julian, Recipient, NHLBI Minority High
27
School Supplement
1998-2001
Mentor, Dr. Zili Zhang, Post-Doctoral Fellow, NC State.
Recipient F32 award, NIAAA
1997-2000
Mentor, Dr. Peng Ye, Post Doctoral Fellow
1998-1999
Mentor-Sponsor, Dr. Carols Vazquez, LSU Pulmonary
Fellow and Recipient Robert Wood Johnson Minority
Fellowship
1999-2001
Mentor, Ms. Perri Prellop, Honors Student in Medicine
1999-2001
Mentor, Mr. Patrick Garvey, Honors Student in Medicine
1999-2001
Mentor, Ms. Christine DeLaune, University of New Orleans
Biology Student
1999-2001
Mentor, Ms. Jennifer Abadie, University of New Orleans
Biology Student
1999-2000
Mentor, Mr. Fred Rodriguez, Undergraduate Student,
Christian Bros. University, Memphis Tennessee
1995-1998
Mentor, Dr. Dinghua Lei, Post-Doctoral Fellow
1997-1998
Mentor, Ms. Meredith Lakey, University of New Orleans,
Biology Program
1997-1998
Mentor, Ms. Tolu Osedipie, University of New Orleans,
Biology Program
1994-1996
Mentor, Mr. Guy Odom, University of New Orleans, Biology
Program
University of Pittsburgh
2003-2006
Mentor, Dr. Florencia McAllister, Post-Doctoral Fellow
2004-2007
Mentor, Dr. Laura McKinley, Post-Doctoral Fellow
2005-2008
Thesis Advisor, Rekha Rapaka MSTP trainee
28
2005-2008
Mentor, Dr. Shean Aujla, Post-Doctoral Fellow
2005-2008
Mentor, Dr. Salman Khan, Post-Doctoral Fellow
2005-present
Thesis Advisor, Christopher Crowe, MSTP trainee
2007-present
Sponsor, Dr. Patricia Dubin; K08HL081537 “IL-23: Actions
and Regulation in Pseudomonas aeruginosa Pulmonary
Infection”
2007-present
Sponsor, Dr. Yvonne Chan, K08HL089189, “Role of
Lipocalin 2 in host defense against Klebsiella pneumoniae
infection”
RESEARCH:
GRANTS: (all dollars in direct costs)
1991-1994
Parker B. Francis Pulmonary Research
Fellowship. $106,000
1993-1994
Stanley S. Scott Cancer Center
Tumor Necrosis Factor and Melanoma Metastasis
$6,000
1995-1996
Cystic Fibrosis Foundation, Transient CD4-depletio prolongs
adenoviral-mediated transgene expression. $30,000
12/93-11/98
Collaborator, NIAAA-AA-92-03
Alcohol-induced modulation of cytokines and
nitric oxide in rat lung. $862,778
2/94-1/98
Collaborator, NIAAA-093-47-01
Ethanol, Nitric Oxide , and Lung Host
Defense. $660,000
1996-1998
Adenoviral Vectors Encoding Toxin Genes for the Treatment
of Prostate Cancer, $106,000 Elsa Pardee Foundation
1997-1998
Cloning and Characterization of the Prostate-Specific
Membrane Antigen Promoter, $15,000. CAGNO
1997-1998
Gene Therapy for Malignant Mesothelioma
$15,000. CAGNO
29
1996-1999
Immunosuppression and Adenovirus-mediated Gene
Transfer $150,000 CF Foundation
1997-1999
Leukemia Society $165,114 "Development of a gene
therapy based bone marrow purging system"
1997-2000
Immunomodulation and Adenoviral-Mediated Gene Transfer
Muscular Dystrophy Association, $152,000
1995-2001
P.I. "Alcohol, Immunosuppression, and TNF"
R29AA10384. NIAAA, $350,000, 1.7 percentile priority score
1999-2001
Mentor, F32- AA05543-01, P.I. Zili Zhang, Ph.D.
1997-2002
Collaborator, "T-lymphocyte Subsets and P. carinii
Pneumonia" (P.I. Judd Shellito)
RO1 HL59724-01, $875,000
1997-2002
Collaborator, "Alcohol, TB, and AIDS"
RO1 AA/AI11760-01, $850,000 (P.I. Carol Mason)
1999-2003
P.I. RO1-HL61721-01. "Non-CD4 Host Defenses against P.
carinii Pneumonia". $250,000
1999-2002
Collaborator, R01 CA81125, (P.I.: Paul Schwarzenberger,
M.D.) "IL-17 and Hematopoiesis". $350,000
2000-2003
P.I. -5P50AA009803-089002 "Alcohol Research Center
Molecular/Cellular Biology Laboratory Core". $875,000 (P.I.
of P50 – Steve Nelson).
1998-2003
Co-Associate Program Director, Tulane/LSU/Charity
General Clinical Research Center, MO1-RRO-5096
2001-2002
3R01HL062052-03S1, Minority Supplement to " "CD8 and
Gamma/delta T cells in P. carinii Pneumonia
1998-2004
RO1-HL62052. "CD8 and Gamma/delta T cells in P. carinii
Pneumonia". $875,000 (direct costs)
30
Active: (Role of PI unless otherwise indicated)
2004-2009
R01-HL62052 "CD8 T cells in P. carinii Pneumonia".
$1,250,000 (direct costs)
2003-2008
RO1-HL61721, “Non-CD4 Host Defense against P. carinii
Pneumonia”, 131 Priority Score (2 percentile).
1/2/03-12/31/08. $1,175,000 (direct costs)
2001-2007
"Alcohol, Immunosuppression, and TACE"
R01-AA10384. NIAAA, $1,000,000.
2004-2009
P01 HL076100-01 “Host Defense Against HIV-related
Pulmonary Infections” (Role: Project Leader; Overall PI:
Judd Shellito). $1,300,000 (direct costs)
2004-2009
R01HL079142 “IL-23 / IL-17 and Lung Host Defense” (4th
percentile)
$1,413,300 (direct costs)
2005-2010
5P30DK072506 "Basic and Clinical Studies of Cystic
Fibrosis" (Role: Project Leader; Overall PI: Ray Frizzell).
$396,590 (direct costs)
2006-2011
P50 HL084932, “Host Factors in Fungal Allergy and Fibrosis”
(Role: PI)
$1,610,212/year
2006-2011
R01 AA016688, “Alcohol, ROS, and Macrophage
Epigenetics”
(Role: PI)
$210,000/year
Pending:
RO1-HL61721-10, “Non-CD4 Host Defense against P. carinii
Pneumonia”, 145Priority Score (14th percentile).
4/1/09-3/31/14. $1,250,000 (direct costs)
RO1-HL61721-10A1, “Non-CD4 Host Defense against P.
carinii Pneumonia”, 120Priority Score (1.6 percentile).
4/1/09-3/31/14. $1,250,000 (direct costs)
31
Grant Reviews:
Served on NIH Review Committee for RFA HL-95013 entitled HIV-Associated Pathogens of the
Lung: Life Cycle Regulation.
Served on NIH SCOR Study Section: Molecular
Mechanisms of Asthma, 1996
Practice Site Visit for University of Florida, Gainesville
General Clinical Research Center
NIH Special Emphasis Panel: Program Project Grants on
Gene Therapy for Cystic Fibrosis, Sept. 1998
NIH Special Emphasis Panel: Program Project Grant on
Cardiovascular Gene Delivery, Dec. 1998
Ad Hoc Review for NIAID, April, 1999
Served on NIH SCOR Study Section: Molecular
Mechanisms of Asthma, 2000
Served on NIH SEP - Program of Excellence in Gene
Therapy, 2000
Asked to serve on NIH AACR-4 Study Section, declined
Served on NIH SEP - NHLBI Program Projects in Gene
Therapy for Heart, Lung, and Blood Disorders, 2001
Served on NIH SEP - NHLBI Center for Fetal Gene Delivery,
2001
Ad Hoc Reviewer, Lung Biology and Pathology Study
Section, CSR, 10/2001.
Named to NIH/CSR Lung Biology and Pathology Study
Section, 2002-2004
NIAAA SEP “Stem Cells in Alcohol-Related Disease” 2002
Named to NIH/CSR Lung, Cellular and Molecular
Immunology (LCMI) Study Section, 2003-2006
Chair, LCMI Study Section, 2006-2007
32
Clinical Trials:
Medi-493 “A randomized, double-blind Placebo- Control trial
of an anti-RSV Monoclonal Antibody in Preventing RSV
Hospitalization” Medimmune, Gaithersburg, MD
(1996-1997)
Consultant, Amgen Community Acquired Pneumonia Study:
Development of Time to Resolution of Morbidity (TRM) as
an outcome measure in Community Acquired Pneumonia
(1994-1995)
The Treatment of Malignant Pleural Mesothelioma with a
Gene Modified Cancer Vaccine: A Phase I Study
(Approved FDA, May 1997)
P.I. "Mechanisms of Alcohol-induced Alterations in TNF in
Human Subjects" $975,000 (direct costs): Health Excellence
Fund - Center for Lung Immunology.
Co-I. VICAL: Phase II study of Allovectin for Malignant
Melanoma
“IL-8, LTC4, and LTB4: Their role in RSV-induced
inflammation and bronchospasm in patients with
bronchiolitis.
2. Seminars and invited lectureships related to your research
Invited Lectures (partial listing):
“Using Adenoviral-mediated Gene Transfer as an
Alternative to Gene Knockout” 1995 Cambridge
Healthtech Institute Meeting on Inflammatory
Cytokine Antagonists.
“Immune Responses to Adenoviral Vectors and Its Impact
on Gene Therapy Trials” Humboldt University, Berlin
Germany. Host: Hans-Dieter Volk, Chief of Medical
Immunology, May 1996
Keynote Speaker, 1997 American Lung Association
“Gene Delivery as a Laboratory Tool”
Meet the Professor, 1997 American Lung Association
“Gene Therapy for Acquired Lung Disease”
33
American Lung Association Post-Graduate Course.
“Gene Therapy to Modulate Lung Immunity”, April, 1998
50th Anniversary NHLBI sponsored Symposium.
“Airway Susceptibility to Infections and the Role of
Cytokines”, American Lung Association Meeting, Chicago,
IL. April, 1998
“Gene Therapy to Modulate Lung Immunity” Pulmonary
Consultant, University of Rochester. February, 1999
“Gene Therapy to Modulate Lung Immunity” Fourth
International Symposium on Infections in the Critically Ill
Patient. Barcelona, February 26-27, 1999
“Immune Reconstitution in HIV Infection: Future Reality or
False Hope” American Lung Association Meeting, San
Diego, CA. April, 1999
"Gene Therapy for lung diseases: promises, realities, and
risks" and "Gene Delivery: Viral and Non-Viral Vectors" at
the 2000 European Respiratory Society Meeting, Florence,
Italy
"Gene Therapy to Modulate Lung Immunity" at the
International Symposium on Progress in Drug Discovery &
Development Sciences, Astra-Zeneca Research Foundation,
Bangalore, India, 2001.
"Acute and Chronic Ethanol and their Effects of TACEmediated Ectodomain Shedding" a satellite meeting of 2001
Research Society on Alcoholism, Montreal, Canada, 2001
entitled "New aspects of immunomodulation by alcohol"
“IL-17 Family Members and Lung Host Defense”.
May 20, 2003 American Thoracic Society Meeting,
Seattle, WA
“IL-17 and Lung Host Defense”
August 26, 2003. Wyeth Pharmaceuticals
Cambridge, MA
“Gene transfer in innate and adaptative immunity for
opportunistic infections - immunotherapeutic and vaccine
strategies” 2003 European Respiratory Society, Vienna,
34
Austria
“HIV immunology and new therapies” Congress of
International Pediatric Pulmonology, Feb. 2004, Lisbon,
Portugal
“Gene Therapy” Congress of International Pediatric
Pulmonology, Feb. 2004, Lisbon, Portugal
“IL-17 Family Members and Inflammation” Dept of
Immunology, University of Pittsburgh, August 2004
“IL-17 Family Members and Inflammation” DNAX, Palo Alto,
CA April 2005
“IL-17 Family Members and Inflammation” Genentech,
South San Francisco CA. June 2006
“IL-17 Family Members and Lung Inflammation” Centocor,
Radnor, PA. June 2006
“IL-17 Family Members and Lung Inflammation”
Zymogenetics, Seattle, WA. August 2006
4. Other research related activities
Pharmaceutical Consulting:
Member, Scientific Advisory Board, Inhale Therapeutics,
San Carlos, CA
Member, Scientific Advisory Board, EnGene, Inc.
Vancouver, BC, Canada
Consultant, Abgenix, Inc., Fremont, CA
Consultant, Amgen-Seattle (formerly Immunex Corp.),
Seattle, WA
Consultant, Zymogenetics, Seattle, WA
Editorial:
Ad Hoc Reviewer: American Journal of Respiratory Cell and
35
Molecular Biology, American Journal of Physiology,
Alcoholism, Journal of Clinical and Laboratory Investigation,
Human Gene Therapy, Journal of Clinical Investigation,
Infection and Immunity, Cancer Research, Pediatrics,
Journal of Experimental Medicine, Journal of Immunology,
Blood, Proceedings of the National Academy Sciences
Editorial Board:
Respiratory Research
Section Editor, Journal Of Immunology
Consulting Editor, Journal of Clinical Investigation
LIST OF CURRENT RESEARCH INTERESTS:
The major goal of my research is to investigate mechanisms of lung host defenses in
normal and immunocompromised hosts. Presently, we are investigating how IL-23 and
IL-17 regulate neutrophil recruitment in response to infectious stimuli in the lung. To
this end we study cellular sources of IL-17A, IL-17F, and IL-22 in the lung as well as
their signaling in response to pulmonary infection. We also have long standing interest
to determine if Th17 cells and their cytokine products contribute to airway destruction in
cystic fibrosis. We also have a long-standing interest in understanding cytokine biology
in the lung through over-expression or dominant negative inhibitor strategies using
somatic gene transfer. In these studies we have identified that sub-populations of
CD8+ T-cells polarized in vivo via cytokine gene transfer have effector activity against
P. carinii. We are presently using gene expression profiling and proteomics to define
this effector activity. We also have a program in developing CD4-independent
vaccination against AIDS-related opportunistic infections.
Current Research interest Keywords:
gene therapy, lung immunology, lung host defenses, tumor necrosis factor,
pneumonia, pneumocystis carinii pneumonia, ethanol, gene expression,
polymerase chain reaction, molecular biology
---------------------------------------------------------------------------------------------------------------------SERVICE
1. University and Medical School
Academic Committees: 1998: Ad Hoc Committee for Biometry and Genetics,
LSUHSC
1998-present: Animal Care Committee
1998-present: GCRC Advisory Committee
36
2001: Chancellor of LSUHSC-New Orleans Search
Committee
2002: Member, Institutional BioSafety Committee
2004-2005: member Search Committee for Director,
Vaccine Center, University of Pittsburgh
National Committees:
Allergy, Immunology, and Inflammation Program Committee,
America Thoracic Society, 1999-2002
Respiratory Committee, American Society of Gene Therapy
2001-present
2. Community Activities:
Care Mobile Asthma Screening Program,
Children’s Hospital of Pittsburgh
3. Continuing Medical Education (CME) activities:
37