Symposium GRK1167: Gene

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Workshop GRK1167: Gene-delivery methods
Date: 2nd of March begin at 9:00 am
Talk: 20 min + 10 min discussion
Location: LIN Matthies Seminar Room
Supervisors: Stefanie Kliche (Tel.: 67-15855 email stefanie.kliche@med.ovgu.de)
Luca Simeoni ( Tel.: 67-17894 email luca.simeoni@med.ovgu.de)
Oliver Stork (Tel. 67-55100 email oliver.stork@ovgu.de)
Topic 1: Chemical methods
Juliane Handschuh
Research paper for:
Xu Wang
Zhihui Zhu
Thomas Mack
Supervisor: Stefanie Kliche
- Overview
- Calcium phosphate transfection
- Gene transfer using liposomes
- Gene transfer using nanoparticles
Topic 2: Physical methods
Nishanth Gopala
- Overview
Research paper for:
Marie-Christin Wanger - Microinjection
Katharina Wex
- Biolistic particle delivery system
Marc Borgmann
- Electroporation
Mateusz Poltorak
- Delivery using ultrasound
Supervisor: Luca Simeoni
Topic 3: Viral methods
Amarendra Reddycherla - Overview
Research paper for:
Annabel Hinderfeldt
- Delivery using Adenoviruses
Barbara Schweitzer
- Delivery using Lentiviruses
Supervisor: Oliver Stork
Overview:
- Papers for the topics will be provided at the end of January by the responsible
supervisor
- Introduce each method
- Compare (if possible) transfection efficiency of primary cells with cell lines
- Suitable for in vivo applications
Research paper:
- Chosen by the student
- From high ranking journals
- Contains in vivo applications (if possible) of this method
If you are not sure whether your selected research paper fulfills these criteria’s, please contact
your supervisor.
In case you cannot find a suitable research paper, please contact your supervisor (we will have
a back up).
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