Workshop GRK1167: Gene-delivery methods Date: 2nd of March begin at 9:00 am Talk: 20 min + 10 min discussion Location: LIN Matthies Seminar Room Supervisors: Stefanie Kliche (Tel.: 67-15855 email stefanie.kliche@med.ovgu.de) Luca Simeoni ( Tel.: 67-17894 email luca.simeoni@med.ovgu.de) Oliver Stork (Tel. 67-55100 email oliver.stork@ovgu.de) Topic 1: Chemical methods Juliane Handschuh Research paper for: Xu Wang Zhihui Zhu Thomas Mack Supervisor: Stefanie Kliche - Overview - Calcium phosphate transfection - Gene transfer using liposomes - Gene transfer using nanoparticles Topic 2: Physical methods Nishanth Gopala - Overview Research paper for: Marie-Christin Wanger - Microinjection Katharina Wex - Biolistic particle delivery system Marc Borgmann - Electroporation Mateusz Poltorak - Delivery using ultrasound Supervisor: Luca Simeoni Topic 3: Viral methods Amarendra Reddycherla - Overview Research paper for: Annabel Hinderfeldt - Delivery using Adenoviruses Barbara Schweitzer - Delivery using Lentiviruses Supervisor: Oliver Stork Overview: - Papers for the topics will be provided at the end of January by the responsible supervisor - Introduce each method - Compare (if possible) transfection efficiency of primary cells with cell lines - Suitable for in vivo applications Research paper: - Chosen by the student - From high ranking journals - Contains in vivo applications (if possible) of this method If you are not sure whether your selected research paper fulfills these criteria’s, please contact your supervisor. In case you cannot find a suitable research paper, please contact your supervisor (we will have a back up).