Titre de la formation Developmental Biology workshop Organisateur de formation : UPMC- Institut Curie Public visé : ● Métiers, fonctions ou postes occupés (ingénieurs, techniciens,…) PhD and Master 2 students ● Conditions d’accès et Pré-requis demandés aux stagiaires : connaissances, niveau d’études et/ou VAE This course is proposed to PhD students who have previously acquired basic knowledge in Developmental Biology ; Student selection is based on application and letter of motivation. Objectifs : ● Objectif général de la formation This module is an intensive laboratory and lecture course which provides a comprehensive coverage of the paradigms, problems and technologies of modern developmental biology. Students receive training in theoretical and experimental aspects of normal cell development as well as abnormal aspects of these processes that may cause birth defects or diseases. They are exposed to a variety of embryonic systems, including genetic model system (C. elegans, Drosophila, zebrafish, mouse) and well-established experimental attributes (frog, chick) using state-of-the-art instrumentation and methodology. Lectures and seminars are given by renowned international researchers on topics including cell specification and differentiation, pattern formation, morphogenesis and organogenesis, intracellular signalling and developmental regulation of gene expression The practicals are designed to reinforce some of the ideas put in the lectures and allow the students to discuss freely with researchers. Contenus : ● programme de la formation. Practicals : C. Elegans : Realisation and observation of mutants, olfactive tests. Zebrafish: Analysis of Nodal mutants. Injection in mutants and analysis of operated embryos. Xenopus : Manipulating embryonic axes, Spemann experiment, mesoderm induction in vitro, Nieuwkoop recombination, gastrulation in vitro. Drosophila : Transgenic embryo analysis : study of cuticule, wing and leg imaginal discs, formation of mecano-sensorial organs. Mouse : Culture of mouse oocytes and early mouse embryos, .Rhombencephale study in mouse, Development of organs derived from the primitive intestine, Embryo and Organ Culture Avian: Whole embryo culture. Intracardiac retroviral injection. Chick-quail grafts. Neural tube electroporation. Neural crest cell culture Seminars : Morphogenetic gradients during development, vertebrate gastrulation, endoderm development and differentiation, early steps in the regionalisation of the vertebrate embryo, neural induction , neural crest development and migration, cell migration during organ morphogenesis, axis formation and segmentation in vertebrates, epigenetic decisions and reproduction in mammals.,germline stem cells, embryonic stem and progenitor cells during development., organ development and cancer, genetic evolution during development, development of marine organisms, stem cell development in plants Organisation matérielle : ● Durée et Rythme de la formation Short session : 2 weeks seminars Long session : 3 weeks practical and 2 weeks seminars ● Nombre Maximal et Minimal de Participants. Short sesssion : 30-35 students Long session : 18 students. Calendrier : ● Dates des sessions 2011 : Short session : October 18th to 30th Long session : September 26th- October 30th One session per year Lieu du cours: Practicals : UPMC, 9 quai St Bernard 75005 Paris Seminars : Institut Curie26 rue d’Ulm75005 Paris Validation : PhD students : scientific paper presentation. Master students : project presentation Frais de participation : None Date limite pour candidater : End of May Contacts Pédagogiques : Claire Fournier-Thibault at UPMC claire.fournier@snv.jussieu.fr Jean-René Huynh at Curie Institute Jean-Rene.Huynh@curie.fr Contacts pour l’ecole doctorale : Devaux Frédéric Devaux at biologie.ens.fr Site web du cours : A venir