Agilent Technologies HPLC (3D) ChemStation Operation

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For exercises the data-,method-,sequence files should be copied in the proper directory, as described in the instructor notes in the Lab-manual. Material used for delivery: Slides in PowerPoint97 Format: Slides\mod1.ppt Student and Instructor Manual in Word Format: Modules\mod1.doc Lab Sessions in Word97 Format: Laboratories\mod1lab.doc How to handle the material: Manuals in word: H8716ast.doc turn on hidden text for instructor notes. Postscript files for printing H8716ast.ps Student manual H8716ainst.ps Instructor manual 8716stcover.ps student manual cover 8716instcover.ps instructor manual cover H8716alab.ps Student lab manual H8716alabinst.ps Instructor lab manual 8716labcover.ps Student lab manual cover 8716instlabcover.ps instructor lab manual cover Content: Module 1: Introduction to the HPLC ChemStation Module 2: Creating a Method – Acquisition * Module 3: Qualitative Data Analysis Module 4: Integration* Module 5: Quantification Module 6: Basic Reporting* Module 7: Sequence and Batch Review* Module 8: Advanced Reporting* Module 9: Spectra Data Analysis* Module 10: Library Searching* *Perform Laboratory ------------------------------------------------------------------------- Module 14: FLD 1100 Section !!!( an Option) not included in Student/Instructor Manual!!! Revision Date:February 2000 Feedback to Deborah Perkins 648-5411 1.Instructor or Delivery Agent skills required to deliver the product: Instructor must have thorough knowledge and understanding of the HPLC ChemStation software A.04 to A.07. The instructor must know how to customize and maintain Windows 95 and Windows NT . Instructor must have an understanding of HPLC theory and applications. Instructor must know and understand how to use spectral data including: how to explain and use the peak purity software; how to use and demonstrate isoabsorbance plotting; and how to build a UV library. Instructor must know both the original and enhanced integrator. Instructor must know how to build calibration tables, perform sequencing (with bracketing and cyclic recalibrations), and use sequence summary reporting. Helpful but not required: Instructor must be able to create customized reports with the custom report generator, commands and macros. HP ChemStation HPLC 3D H8716A Prerequisite: Understanding of Win95 or WinNT and basic of HPLC Target Audience: User of the HPChemStation module. Rev.A06.XX with spectra Goal for this training: After this training the student will be able to use the HPChemStation with the basic funktions. This training is scheduled with PC only. 1.Day ChemStation structure and instrument configuration Chemstation overview Method creation for acquisition Instrument control Data analyse 2.Day Calibration Basic reporting Setup a sequence Recalibration and Batch review Avanced reporting 3.Day Spectra handling Isoabsorbance plot - 3D plot Peak purity Build a spectra library " ß $ 1 : F • — ¦ ³ ´ À ¸ Ò { 9 ˜ : H { Š È Þ Š ‘ " ! z ¨ { à ÷ " — þ ‚ ‰ Þ 5 •CJ( CJ # $ : ! 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<xsd:enumeration value="Republic of Korea"/> <xsd:enumeration value="Russia"/> <xsd:enumeration value="Singapore"/> <xsd:enumeration value="Slovenia"/> <xsd:enumeration value="Socialist Countries"/> <xsd:enumeration value="South Africa"/> <xsd:enumeration value="Spain"/> <xsd:enumeration value="Sweden"/> <xsd:enumeration value="Switzerland"/> <xsd:enumeration value="Turkey"/> <xsd:enumeration value="United Kingdom"/> <xsd:enumeration value="United States"/> <xsd:enumeration value="United States/Canada"/> <xsd:enumeration value="Universal"/> </xsd:restriction> </xsd:simpleType> </xsd:element> <xsd:element name="ReleaseDate" ma:index="18" nillable="true" ma:displayName="Release Date" ma:format="DateTime" ma:internalName="ReleaseDate"> <xsd:simpleType> <xsd:restriction base="dms:DateTime"/> </xsd:simpleType> </xsd:element> <xsd:element name="NativeApplication" ma:index="19" nillable="true" ma:displayName="Native Application" ma:format="Dropdown" ma:internalName="NativeApplication"> <xsd:simpleType> <xsd:restriction base="dms:Choice"> <xsd:enumeration value="AmiPro"/> <xsd:enumeration value="CorelDraw"/> <xsd:enumeration value="Designer"/> <xsd:enumeration value="Excel"/> <xsd:enumeration value="FrameMaker"/> <xsd:enumeration value="Freehand"/> <xsd:enumeration value="Illustrator"/> <xsd:enumeration value="InDesign"/> <xsd:enumeration value="Interleaf"/> <xsd:enumeration value="Lectora"/> <xsd:enumeration value="Lotus123"/> <xsd:enumeration value="Microsoft Word"/> <xsd:enumeration value="MS Office"/> <xsd:enumeration value="MS Windows Media Player"/> <xsd:enumeration value="Not applicable"/> <xsd:enumeration value="PageMaker"/> <xsd:enumeration value="PCL"/> <xsd:enumeration value="PDF"/> <xsd:enumeration value="Photoshop"/> <xsd:enumeration value="Picture Publisher"/> <xsd:enumeration value="PowerPoint"/> <xsd:enumeration value="Quark"/> <xsd:enumeration value="Schema"/> </xsd:restriction> </xsd:simpleType> </xsd:element> <xsd:element name="PageCount" 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value="Superspher Columns"/> <xsd:enumeration value="SureSelect"/> <xsd:enumeration value="Synthesizer"/> <xsd:enumeration value="Syringe Filters"/> <xsd:enumeration value="Syringes"/> <xsd:enumeration value="Systems"/> <xsd:enumeration value="Thermostat Column Compartments"/> <xsd:enumeration value="TMR 8900"/> <xsd:enumeration value="TMRAqua70"/> <xsd:enumeration value="Transducers"/> <xsd:enumeration value="Turbo Pumping Systems"/> <xsd:enumeration value="Turbo Pumps"/> <xsd:enumeration value="UV-VIS Accessories"/> <xsd:enumeration value="UV-VIS Compliance"/> <xsd:enumeration value="UV-Vis Standards & Reagents"/> <xsd:enumeration value="UV-VIS Systems"/> <xsd:enumeration value="UV-VIS-NIR"/> <xsd:enumeration value="Vacuum Manifolds, Parts and Accessories"/> <xsd:enumeration value="Vacuum Supplies"/> <xsd:enumeration value="Vacuum Technologies"/> <xsd:enumeration value="Vials"/> <xsd:enumeration value="VS Series Automatic Leak Detectors"/> <xsd:enumeration value="Workstations"/> <xsd:enumeration value="X-ray Source"/> <xsd:enumeration value="ZORBAX"/> </xsd:restriction> </xsd:simpleType> </xsd:element> </xsd:sequence> </xsd:extension> </xsd:complexContent> </xsd:complexType> </xsd:element> <xsd:element name="Product" ma:index="26" nillable="true" ma:displayName="Product" ma:hidden="true" ma:internalName="Product"> <xsd:complexType> <xsd:complexContent> <xsd:extension base="dms:MultiChoice"> <xsd:sequence> <xsd:element name="Value" maxOccurs="unbounded" minOccurs="0" nillable="true"> <xsd:simpleType> <xsd:restriction base="dms:Choice"> <xsd:enumeration value="G2912A"/> <xsd:enumeration value="G2613A"/> <xsd:enumeration value="G2614A"/> <xsd:enumeration value="G2630A"/> <xsd:enumeration value="G2630B"/> <xsd:enumeration value="G2855B"/> <xsd:enumeration value="G2888A"/> <xsd:enumeration value="G2913A"/> <xsd:enumeration value="G2916A"/> </xsd:restriction> </xsd:simpleType> </xsd:element> </xsd:sequence> </xsd:extension> <• ” ƒ „ … † ‡ ˆ ‰ Š ‹ Œ • Ž • • ‘ ’ “ • 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( E u r o p e ) – A f t e r h i s t r a i n i n g t h e s t u d e n t w i l l b e b l e t o u s e t h e H P L C C h e m S t a t i o n i t h t h e b a s i c f u n c t i o n s . T h i s r a i n i n g i s s c h e d u l e d w i t h P C o n l y . E u r o p e ) < ; # H P L C 2 D C h e m S t a t i o n o f t w a r e ; # H P L C C h e m S t a t i o n o f t w a r e ; # L E B E R , B I L L ( A i t t l e F a l l s , e x 1 ) ; # C h r o m a t o g r a p h y D a t a S y s t e m s ; # ‰ h t t p s : / / e x t r a n e t . c h e m . a g i l e n t . c o m / W o r k s p a c e s / T r a i n i n g / P u b l i c / 0 2 1 1 8 6 , h t t p s : / / e x t r a n e t . c h e m . a g i l e n t . c o m / W o r k s p a c e s / T r a i n i n g / P u b l i c / 0 2 1 1 8 6 u h t t p s : / / e x t r a n e t . c h e m . a g i l e n t . c o m / L i b r a r y / T r a i n i n g / P u b l i c , h t t p s : / / e x t r a n e t . c h e m . a g i l e n t . c o m / L i b r a r y / T r a i n i n g / P u b l i c O 0 x 0 1 0 1 0 0 9 F 5 C 1 4 F 1 C F 5 8 4 7 C 7 B B B A D A 9 A 8 6 3 7 D E A B 0 1 5 5 0 0 7 3 7 B 7 7 E 8 9 0 8 2 3 A 4 E 8 1 E 8 5 6 5 0 F 4 E A 2 A 6 B & B A L D W I N , V I C T O R I A ( N o n A - A m e r i c a s /xsd:complexContent> </xsd:complexType> </xsd:element> <xsd:element name="IndustryGroup" ma:index="27" nillable="true" ma:displayName="Industry Group" ma:description="Select only the Industry Group that is the primary focus of this content." ma:hidden="true" ma:internalName="IndustryGroup"> <xsd:complexType> <xsd:complexContent> <xsd:extension base="dms:MultiChoice"> <xsd:sequence> <xsd:element name="Value" maxOccurs="unbounded" minOccurs="0" nillable="true"> <xsd:simpleType> <xsd:restriction base="dms:Choice"> <xsd:enumeration value="BioPharma"/> <xsd:enumeration value="Clinical Research"/> <xsd:enumeration value="Energy & Fuels"/> <xsd:enumeration value="Environmental"/> <xsd:enumeration value="Food Testing & Agriculture"/> <xsd:enumeration value="Forensics & Drug Testing"/> <xsd:enumeration value="Genomics"/> <xsd:enumeration value="Geochemistry, Mining & Metals"/> <xsd:enumeration value="Homeland Security"/> <xsd:enumeration value="Integrated Biology"/> <xsd:enumeration value="Materials Testing & Research"/> <xsd:enumeration value="Metabolomics"/> S C I t u b t a w t ( S S L <xsd:enumeration value="Pharmaceuticals"/> <xsd:enumeration value="Proteomics & Protein Sciences"/> <xsd:enumeration value="Semiconductor Analysis"/> <xsd:enumeration value="Specialty Chemicals"/> <xsd:enumeration value="Vacuum Solutions"/> </xsd:restriction> </xsd:simpleType> </xsd:element> </xsd:sequence> </xsd:extension> </xsd:complexContent> </xsd:complexType> </xsd:element> <xsd:element name="IndustryType" ma:index="28" nillable="true" ma:displayName="Industry Type" ma:hidden="true" ma:internalName="IndustryType"> <xsd:complexType> <xsd:complexContent> <xsd:extension base="dms:MultiChoice"> <xsd:sequence> <xsd:element name="Value" maxOccurs="unbounded" minOccurs="0" nillable="true"> <xsd:simpleType> <xsd:restriction base="dms:Choice"> <xsd:enumeration value="AgChem"/> <xsd:enumeration value="Agriculture"/> <xsd:enumeration value="Consumer Products"/> <xsd:enumeration value="Disease Discovery"/> <xsd:enumeration value="Drug Development"/> <xsd:enumeration value="Drug Discovery"/> <xsd:enumeration value="Drug Manufacturing/QA/QC"/> <xsd:enumeration value="Drug Testing"/> <xsd:enumeration value="Environmental"/> <xsd:enumeration value="Foods & Flavors"/> <xsd:enumeration value="Forensics"/> <xsd:enumeration value="Fuel Cells"/> <xsd:enumeration value="Genomics"/> <xsd:enumeration value="Homeland Security"/> <xsd:enumeration value="Hydrocarbon Processing"/> <xsd:enumeration value="Nucleic Acid Analysis"/> <xsd:enumeration value="Production-QA/QC"/> <xsd:enumeration value="Proteomics"/> <xsd:enumeration value="Semiconductor"/> <xsd:enumeration value="Specialty Chemical"/> </xsd:restriction> </xsd:simpleType> </xsd:element> </xsd:sequence> </xsd:extension> </xsd:complexContent> </xsd:complexType> </xsd:element> <xsd:element name="Industry" ma:index="29" nillable="true" ma:displayName="Industry" ma:hidden="true" ma:internalName="Industry"> <xsd:complexType> <xsd:complexContent> <xsd:extension base="dms:MultiChoice"> <xsd:sequence> <xsd:element name="Value" maxOccurs="unbounded" minOccurs="0" nillable="true"> <xsd:simpleType> <xsd:restriction base="dms:Choice"> <xsd:enumeration value="Air"/> <xsd:enumeration value="Analysis of Process Chemicals"/> <xsd:enumeration value="Antibacterial Drug Residues"/> <xsd:enumeration value="Bioanalysis"/> <xsd:enumeration value="Biological Warfare Agents (BWA)"/> <xsd:enumeration value="Chemical Warfare Agents (CWA)"/> <xsd:enumeration value="Components Analysis"/> <xsd:enumeration value="Contamination Control"/> <xsd:enumeration value="Environmental Monitoring"/> <xsd:enumeration value="Flavors"/> <xsd:enumeration value="Food"/> <xsd:enumeration value="Fragrances"/> <xsd:enumeration value="Natural Compounds & Additives"/> <xsd:enumeration value="Pesticides & Residues"/> <xsd:enumeration value="Silicon Wafer Analysis for Organic Contaminants"/> <xsd:enumeration value="Soil & Sediment"/> <xsd:enumeration value="Toxic Industrial Chemicals (TIC)"/> <xsd:enumeration value="Ultra-pure Water Analysis"/> <xsd:enumeration value="Water, Soil & Sediment"/> </xsd:restriction> </xsd:simpleType> </xsd:element> </xsd:sequence> </xsd:extension> </xsd:complexContent> </xsd:complexType> </xsd:element> </xsd:schema> <xsd:schema targetNamespace="3a52aef6-3c65-41ed-96e3-cac50d1c25cd" elementFormDefault="qualified" xmlns:xsd="http://www.w3.org/2001/XMLSchema" xmlns:dms="http://schemas.microsoft.com/office/2006/documentManagement/ty pes"> <xsd:import namespace="http://schemas.microsoft.com/office/2006/documentManagement/ty pes"/> <xsd:element name="Abstract" ma:index="5" ma:displayName="Abstract" ma:description="A summary of publication's most important points, presented in paragraph form." ma:internalName="Abstract" ma:readOnly="false"> <xsd:simpleType> <xsd:restriction base="dms:Note"/> </xsd:simpleType> </xsd:element> </xsd:schema> <xsd:schema targetNamespace="ee42ffa4-88aa-408d-9f63-e2d1068a6810" elementFormDefault="qualified" xmlns:xsd="http://www.w3.org/2001/XMLSchema" xmlns:dms="http://schemas.microsoft.com/office/2006/documentManagement/ty pes"> <xsd:import namespace="http://schemas.microsoft.com/office/2006/documentManagement/ty pes"/> <xsd:element name="MainCat" ma:index="21" nillable="true" ma:displayName="MainCat" ma:format="Dropdown" ma:internalName="MainCat"> <xsd:simpleType> <xsd:restriction base="dms:Choice"> <xsd:enumeration value="Products & Services"/> <xsd:enumeration value="Education & Events"/> <xsd:enumeration value="Solutions"/> <xsd:enumeration value="News Releases"/> <xsd:enumeration value="Technical Support"/> </xsd:restriction> </xsd:simpleType> </xsd:element> <xsd:element name="MidCat" ma:index="36" nillable="true" ma:displayName="MidCat" ma:description="Defines the section that this page will appear in the "Narrow Your Result" section of the general site search results." ma:format="Dropdown" ma:internalName="MidCat"> <xsd:simpleType> <xsd:restriction base="dms:Choice"> <xsd:enumeration value="Chemical Analysis"/> <xsd:enumeration value="Classroom Training Courses"/> <xsd:enumeration value="Columns & Supplies"/> <xsd:enumeration value="Downloads and Utilities"/> <xsd:enumeration value="e-Seminars"/> <xsd:enumeration value="Events"/> <xsd:enumeration value="FAQs"/> <xsd:enumeration value="Installation and Maintenance Videos"/> <xsd:enumeration value="Illustrated Parts Breakdowns"/> <xsd:enumeration value="Informatics and Software"/> <xsd:enumeration value="Instruments & Systems"/> <xsd:enumeration value="Life Sciences"/> <xsd:enumeration value="Reagents, Standards & Kits"/> <xsd:enumeration value="Software Familiarization Videos"/> <xsd:enumeration value="Parts Information"/> <xsd:enumeration value="Pharmaceuticals"/> <xsd:enumeration value="Services"/> <xsd:enumeration value="Support Services"/> <xsd:enumeration value="..."/> </xsd:restriction> </xsd:simpleType> </xsd:element> </xsd:schema> <xsd:schema targetNamespace="d60c28fc-3d14-46f3-b174-9a10a2a6c6f4" elementFormDefault="qualified" xmlns:xsd="http://www.w3.org/2001/XMLSchema" xmlns:dms="http://schemas.microsoft.com/office/2006/documentManagement/ty pes"> <xsd:import namespace="http://schemas.microsoft.com/office/2006/documentManagement/ty pes"/> <xsd:element name="Analytical_x0020_Technique" ma:index="37" nillable="true" ma:displayName="Analytical_x0020_Technique" ma:format="Dropdown" ma:internalName="Analytical_x0020_Technique" ma:readOnly="false"> <xsd:simpleType> <xsd:restriction base="dms:Choice"> <xsd:enumeration value="Atomic Absorption (AA)"/> <xsd:enumeration value="Capillary Electrophoresis (CE)"/> <xsd:enumeration value="CE/MS"/> <xsd:enumeration value="Dissolution"/> <xsd:enumeration value="Fluorescence Spectroscopy"/> <xsd:enumeration value="FTIR"/> <xsd:enumeration value="GC"/> <xsd:enumeration value="GC Analyzer"/> <xsd:enumeration value="GC QQQ"/> <xsd:enumeration value="GC Q-TOF"/> <xsd:enumeration value="GC x 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<xsd:enumeration value="Biodiesel/Biogas", e x 1 ) T r a i n i n g M a t e r i a l S y s t e m A c c o u n t L i b r a r y /> <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration <xsd:enumeration value="Biofuel(s)"/> value="Biological 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xmlns:odoc="http://schemas.microsoft.com/office/internal/2005/internalDoc umentation"> <xsd:import namespace="http://purl.org/dc/elements/1.1/" schemaLocation="http://dublincore.org/schemas/xmls/qdc/2003/04/02/dc.xsd" /> <xsd:import namespace="http://purl.org/dc/terms/" schemaLocation="http://dublincore.org/schemas/xmls/qdc/2003/04/02/dcterms .xsd"/> <xsd:element name="coreProperties" type="CT_coreProperties"/> <xsd:complexType name="CT_coreProperties"> <xsd:all> <xsd:element ref="dc:creator" maxOccurs="1" ma:index="9" ma:displayName="Author"/> <xsd:element ref="dcterms:created" minOccurs="0" maxOccurs="1"/> <xsd:element ref="dc:identifier" minOccurs="0" maxOccurs="1"/> <xsd:element name="contentType" minOccurs="0" maxOccurs="1" type="xsd:string" ma:index="35" ma:displayName="Content Type"/> <xsd:element ref="dc:title" maxOccurs="1" ma:index="1" ma:displayName="Title"/> <xsd:element ref="dc:subject" minOccurs="0" maxOccurs="1"/> <xsd:element ref="dc:description" minOccurs="0" 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The application is responsible for updating this value after each revision. </xsd:documentation> </xsd:annotation> </xsd:element> <xsd:element name="lastModifiedBy" minOccurs="0" maxOccurs="1" type="xsd:string"/> <xsd:element ref="dcterms:modified" minOccurs="0" maxOccurs="1"/> <xsd:element name="lastPrinted" minOccurs="0" maxOccurs="1" type="xsd:dateTime"/> <xsd:element name="contentStatus" minOccurs="0" maxOccurs="1" type="xsd:string"/> </xsd:all> </xsd:complexType> </xsd:schema> </ct:contentTypeSchema>

Agilent Technologies HPLC (3D) ChemStation Operation

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