1 Comparison of field measurements of canopy parameters, and BPMS-derived values of the same parameters (‘validation’). A large part of this thesis is concerned with the analysis of structural information derived from the scattered radiation field simulated using 3D BPMS representations of measured plant canopies. These canopies are constructed using the manually measured key plant parameters described in XXX. In order that we may have faith in any BPMS simulation results, and information derived thereof, it is vital that the BPMS data are subjected to some form of validation, quality assessment and sensitivity analysis. To make quantitative comparisons between field-measured1 and BPMS-dervied values of key canopy indicators such as LAI and directional reflectance it is imperative that any potential sources of bias are isolated. If this can be done, these sources may either be removed, or at the least compensated for in some way. The first stage of experimentation is therefore a direct comparison of BPMS derived canopy parameters and their field-measured equivalents. BPMS canopy representations Validation is carried out using the BPMS data of barley, followed by sugar beet, to compare canopy structural and radiometric parameters. 3D geometric barley canopies have been created for a number of dates, and for three separate fields, with the planting densities and row spacings taken from values measured in the field. These simulated canopies are derived from the measurements of a number of individual plants. The probability of occurrence of any particular plant within the BPMS-generated canopy is random: this agrees with the measured canopies in terms of the relative occurrences of one, two or three tillered plants. This does not take into account the possibility of bias in measurement e.g. a favouring of selecting one and two tillered plants over higher orders due to the difficulty of extracting the larger plants cleanly from the ground. 1 Although both the BPMS plant parameters and other measurements such as those of directional reflectance and LAI were made in the field, we will use ‘field’ or ‘field-measured’ to mean non-BPMS, and vice-versa. The measured plants are ‘cloned’ to create a virtual canopy. By this it is meant that the plants are copied, rotated randomly about the vertical and distributed according to a measured planting distribution. The plants are cloned over an area sufficiently large to prevent ‘edge effects’ (anomalies caused by light rays escaping the canopy unimpeded at the edges becoming significant in bi-directional reflectance simulations). The linear dimensions of the model canopy are determined by the requirement that the camera be positioned sufficiently high above the canopy to allow a view zenith angle of 70o to be achieved without the viewing point falling below the level of the top of the canopy. To vary view zenith, the camera is rotated in the vertical plane about a point on the ground at the centre of the canopy, as if on a boom of length L i.e. L h cos where || 70o and h is canopy height (see Fig. XX). In this case, the canopy must be a minimum of h tan() on a side. In actual fact, the plants were distributed over an area of quadruple the size (twice the length and width) that is actually viewed by the camera. In this way, edge effects are completely avoided. If an aperture of finite size is used (i.e. not a pinhole camera) any off-nadir view will suffer from projection effects (the projection of a square area onto the image plane will be tapezoidal). To ensure the same area of surface is viewed from any view zenith angle an orthogonal camera model is used. view zenith angle L h Directional reflectance simulations Spectral directional hemispherical reflectance was simulated for view zenith angles from –70o to 70o in 10o steps in both the solar principle and cross-principle planes, to match the PSII radiometer measurements. Sampling is increased to every 2o for the 5o either side of the hotspot direction. The reflectances of the various canopy elements such as stem, leaf and soil, are taken from measurements made in the field. Transmittance values are taken from the PROSPECT leaf reflectance model (Jacquemoud and Baret, 1992??). Simulations are carried out at the mean solar zenith and azimuth angles of the time of PSII measurements made in the field. The sun position is also calculated from the date, time, latitude and longitude of the observations to provide a means of verifying the measured solar position. The calculated values are likely to be more accurate than the measurements made in the field, as they were made using a hand-held compass (azimuth) and clinometer (zenith). A Zibbordi-Voss sky radiance model is used to create a map of direct and diffuse solar illumination in order to make the simulations as accurate as possible to the original conditions. The sky radiance is simulated for the location and time of the measurements. Reflectance is simulated at 45 wavelengths varying from 450 to 900nm in steps of 10nm using both direct and diffuse sampling. Bidirectional reflectance is defined as the ratio of surface reflectance to a lambertian plane, so to obtain bidirectional reflectance values, the reflectance of a lambertian plane is simulated under the same viewing and illumination conditions as the vegetation canopy. The simulated canopy reflectance is then divided by that of the lambertian surface to give bidirectional reflectance in much the same way as with the radiometer data. Simulated bidirectional reflectance is then compared to that of the PSII measurements for the same dates and times and spectral bands, and any the similarity (or difference) described and quantified. Leaf Area Index (LAI) The LAI of the modelled canopies is calculated by summing the total one sided area of the stem and leaf canopy primitives over unit area within the canopy. These values are compared with the values made in the field using the Licor LAI-2000 instrument. The LAI-2000 instrument has number of known limitations caused by its design: LAI is understimated in the case of sparse canopies. Error can also be introduced if there is a strong row element to the crop which is not sufficiently sampled by taking measurements along transects at different directions relative to the crop row orientation. To avoid this, LAI values are derived from 3 separate transects at different locarions and orientations within the field, each containing 10 separate LAI measurements. This is carried out several times in each field to arrive at a final LAI measurement. In addition, conditions of strong direct sunlight are not ideal for LAI measurement due to the model the LAI-2000 instrument uses. Masks are used to mask out the operator and the suns direct rays in this case, but all these factors must be considered when comparing the LAI values with the BPMS values. These effects are all be much reduced in the denser canopies. Destructive LAI measurements were made in the field by removing all leaves from plants contained within a fixed ground area, laying them flat, and photographing them. The digitised photos were then classified to allow calculation of the total leaf area. These measurements are also compared with the BPMS and LAI-2000 measurements to give a third estimate. % cover An estimate of % cover for each canopy can be made from scanned nadir photographs of the various canopies taken in the field. By performing an HSI transformation on the images and thresholding the hue channel, the proportions of leaf and soil area within the image can be estimated. If the negative size and scanning resolution are known, % cover can be simply calculated. To highlight the visible soil areas an RGB to HSI transformation was performed on the images. The soil displayed a far higher signal in the hue channel than the vegetation which enabled thresholds to be applied to isolate the visible soil components. Figure XXa and XXb show an example BPMS values of % cover are calculated for each BPMS canopy by simulating the reflectance at nadir using only diffuse illumination (to prevent shadowing within the canopy). The material usage statistics give a direct values of % cover. These values are compared with the values estimated from the nadir photography. Joint gap probability The gap probability of a canopy is the probability that a ray of light entering the canopy at an angle i, will reach a level z within the canopy and then be scattered. It is an important structural parameter, controlled by the density and clumpiness (or ‘gapiness’)of the canopy and the number density, orientation and scattering cross-section of the canopy elements. P(z), the probability of reaching a depth z in the canopy from the top of the canopy (z=0) is e(Ez/o) where E = NQ (N=number density, Q=extinction efficiency, and o = cos(i) (Hapke, B. (1981) Reflectance spectroscopy I, JGR, 86 B4, pp3039-3054). The joint gap probability is the probability that a ray of light reaching a depth z within the canopy will experience a single scattering event and then escape from the canopy along a separate path without being scattered further. In most cases this probability will be of identical form as for the downward journey, but with P(z’) = e(Ez/) , = cos(r). In the case where the phase angle is small, and hence the exitant path is close to the incident direction, the light is bound to escape preferentially, having already found a path to depth z in the canopy, hence P(z’) is unity. This is known as the opposition, or hot-spot, effect. Increased clumpiness in a canopy leads to an increase in the probability of a gap through the canopy to a depth z. This in turn leads to both an increase in the amount of scattering from vegetation layers lower in the canopy, and an increase in the number of clumps of vegetation from which little radiation is transmitted (Goel, N. S. (19XX) Inversion of canopy reflectance models for estimation of biophysical parameters from reflectance data, Rem. Sens. Rev., pp205-251). This will tend to increase the amount of radiation scattered back towards the observer. Hence the importance of gap probabilty in controlling the observed vegetation structural characteristics. Joint gap probability can be estimated from BPMS simulations at nadir viewing and illumination. It can also be determined from LAI-2000 measurements, and, where possible, from hemispherical photography. If the focal length and FOV of the camera are known, we can create an angle map of the camera view, and then map this onto the hemispherical photographs. If we can then estimate where the gaps in the canopy are through classification of the images, the gap probability can be estimated. Sensitivity analysis Analysis is carried out to examine the sensitivity of the BPMS models to small variations in a number of measured parameters. Small errors or biases in measurement may cause quite large discrepancies in LAI, % cover or joint gap probability. The difficulty of accurately characterising tiller zenith angles once a plant has been uprooted may cause the % cover and gap probability estimates to be inaccurate. The tendency is to understimate the tiller zenith angles, and to assume the tillers lie almost in a plane: uprooting the plants and laying them flat to assist measurement will tend to maks azimuthal variations. These errors are likely to cause understimates of % cover (particularly if the tiller zenith angles are understimated), and will introduce errors into any simulation experiment. Error in the characterisation of plant density in particular, and row width (variability along row) to a lesser extent will cause both the %cover and LAI to bs incorrect, This can be rectified by measuring plant density as far as possible from the nadir photography. In addition there is the difficulty of assessing the probability of occurrence of plants with different numbers of tillers within the simulated canopy. Again, this can be estimated as far as possible from the nadir photography, but failure to represent this accurately will not just affect the % cover and LAI, but it will affect the clumpiness of the canopy quite considerably. There are other factors to consider, such as the characterisation of leaf twist along the leaf length as a linear function from the base to the tip, which is not necessarily very realistic. This will affect % cover and the leaf projection function (G). However it is more difficult to perform sensitivity analysis on this type of parameter due to the difficulty of characterising the twist in the first place. It cannot be measured in the same way as the the leaf and stem angles, and must be visually estimated. As far as possible, comparisons between the BPMS representations and the measurements made using the LAI-2000, the PSII radiometer and other methods should come from the same dates. This is not always possible due to the restrictions of weather over the summer: there are BPMS data for days when no radiometry and/or LAI measurements could be made. In most cases, however, there are LAI and PSII measurements data from within a few days either side of the BPMS data. Whilst not ideal, this should be close enough for the purposes of comparison of structural parameters.