miRCURY
LNATM
Selected Publications:
microRNA detection probes for in situ hybridization
LNA™ in situ hybridization, Northern blotting and microarray analysis (miRCURY LNA™
microRNA Array):
The expression of microRNA miR-107 decreases early in Alzheimer's disease and may accelerate
disease progression through regulation of beta-site amyloid precursor protein-cleaving enzyme 1.
Wang WX, Rajeev BW, Stromberg AJ, Ren N, Tang G, Huang Q, Rigoutsos I, Nelson PT. J
Neurosci. 2008 Jan 30;28(5):1213-23.
MicroRNA expression and regulation in mouse uterus during embryo implantation. Hu SJ, Ren G, Liu
JL, Zhao ZA, Yu YS, Su RW, Ma XH, Ni H, Lei W, Yang ZM. J Biol Chem. 2008 Jun 13.
MicroRNA expression in the adult mouse central nervous system. Bak M, Silahtaroglu A, Møller M,
Christensen M, Rath MF, Skryabin B, Tommerup N, Kauppinen S.RNA. 2008 Mar;14(3):432-44. Epub
2008 Jan 29.
MicroRNAs: novel regulators involved in the pathogenesis of Psoriasis? Sonkoly E, Wei T, Janson
PC, Sääf A, Lundeberg L, Tengvall-Linder M, Norstedt G, Alenius H, Homey B, Scheynius A, Ståhle
M, Pivarcsi A. PLoS ONE. 2007 Jul 11;2(7):e610.
Altered retinal microRNA expression profile in a mouse model of retinitis pigmentosa. Loscher CJ,
Hokamp K, Kenna PF, Ivens AC, Humphries P, Palfi A, Farrar GJ. Genome Biol. 2007;8(11):R248.
LNA™ in situ hybridization using Double DIG-labeled probes
Specific requirements of MRFs for the expression of muscle specific microRNAs, miR-1, miR-206 and
miR-133. Sweetman D, Goljanek K, Rathjen T, Oustanina S, Braun T, Dalmay T, Münsterberg A.
Dev Biol. 2008 Jun 21.
LNA™ in situ hybridization and immunostaining
Identification and characterization of microRNAs expressed in the mouse eye. Karali M, Peluso I,
Marigo V, Banfi S. Invest Ophthalmol Vis Sci. 2007 Feb;48(2):509-15.
The chromatoid body of male germ cells: Similarity with processing bodies and presence of Dicer and
microRNA pathway components. Kotaja,Noora, Bhattacharyya,Suvendra N. , Jaskiewicz,Lukasz ,
Kimmins,Sarah, Parvinen,Martti, Filipowicz,Witold, Sassone-Corsi,Paolo. Proc Natl Acad Sci U.S.A.
2006, 103 (8), 2647 – 2652.
A brain-specific microRNA regulates dendritic spine development. Schratt GM, Tuebing F, Nigh EA,
Kane CG, Sabatini ME, Kiebler M, Greenberg ME. Nature. 2006 Jan 19;439(7074):283-9. Erratum in:
Nature. 2006 Jun 15;441(7095):902.
MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat
myogenic cells. Joan C. Ritland Politz, Fan Zhang, and Thoru Pederson. Proc Natl Acad Sci U S A.
2006 Dec 12;103(50): 18957-18962.
LNA™ in situ hybridization - FFPE samples
In situ detection of precursor and mature microRNAs in paraffin embedded, formalin fixed tissues and
cell preparations. Nuovo GJ. Methods. 2008 Jan;44(1):39-46.
Altered MicroRNA Expression Confined to Specific Epithelial Cell Subpopulations in Breast Cancer.
L. F. Sempere, M. Christensen, A. Silahtaroglu, M. Bak, C. V. Heath, G. Schwartz, W. Wells, S.
Kauppinen, and C. N. Cole. Cancer Res. 67, 11612-11620 (2007)
An androgen-regulated miRNA suppresses Bak1 expression and induces androgen-independent
growth of prostate cancer cells. Shi XB, Xue L, Yang J, Ma AH, Zhao J, Xu M, Tepper CG, Evans
CP, Kung HJ, deVere White RW. Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19983-8.
RAKE and LNA-ISH reveal microRNA expression and localization in archival human brain. P.T.
Nelson, D. A. Baldwin, W. P. Kloosterman, S. Kauppinen, R. H.A. Plasterk, and Z. Mourelatos. RNA
12, 187-191 (2006).
LNA™ in situ hybridization - cryosections /frozen sections
Members of the miRNA-200 family regulate olfactory neurogenesis. Choi PS, Zakhary L, Choi WY,
Caron S, Alvarez-Saavedra E, Miska EA, McManus M, Harfe B, Giraldez AJ, Horvitz RH, Schier AF,
Dulac C. Neuron. 2008 Jan 10;57(1):41-55.
A skin microRNA promotes differentiation by repressing 'stemness'. Yi R, Poy MN, Stoffel M, Fuchs E.
Nature. 2008 Mar 13;452(7184):225-9.
Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections. Obernosterer G,
Martinez J, Alenius M. Nat Protoc. 2007;2(6):1508-14.
Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked
nucleic acid probes and tyramide signal amplification. Silahtaroglu AN, Nolting D, Dyrskjøt L,
Berezikov E, Møller M, Tommerup N, Kauppinen S. Nat Protoc. 2007;2(10):2520-8.
miR-200b mediates post-transcriptional repression of ZFHX1B. Christoffersen NR, Silahtaroglu A,
Orom UA, Kauppinen S, Lund AH. RNA. 2007 Aug;13(8):1172-8.
MicroRNA regulation of cyclooxygenase-2 during embryo implantation. Chakrabarty A, Tranguch S,
Daikoku T, Jensen K, Furneaux H, Dey SK. Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):151449.
LNA™ in situ hybridization - cells
Distinctive patterns of microRNA expression associated with karyotype in acute myeloid leukaemia.
Dixon-McIver A, East P, Mein CA, Cazier JB, Molloy G, Chaplin T, Andrew Lister T, Young BD,
Debernardi S. PLoS ONE. 2008 May 14;3(5):e2141.
In situ detection of precursor and mature microRNAs in paraffin embedded, formalin fixed tissues and
cell preparations. Nuovo GJ. Methods. 2008 Jan;44(1):39-46.
An androgen-regulated miRNA suppresses Bak1 expression and induces androgen-independent
growth of prostate cancer cells. Shi XB, Xue L, Yang J, Ma AH, Zhao J, Xu M, Tepper CG, Evans CP,
Kung HJ, deVere White RW. Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19983-8.
MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat
myogenic cells. Joan C. Ritland Politz, Fan Zhang, and Thoru Pederson. Proc Natl Acad Sci U S A.
2006 Dec 12;103(50): 18957-18962.
A brain-specific microRNA regulates dendritic spine development. Schratt GM, Tuebing F, Nigh EA,
Kane CG, Sabatini ME, Kiebler M, Greenberg ME. Nature. 2006 Jan 19;439(7074):283-9. Erratum in:
Nature. 2006 Jun 15;441(7095):902.
LNA™ in situ hybridization - whole mount
MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous
system. Kapsimali M, Kloosterman WP, de Bruijn E, Rosa F, Plasterk RH, Wilson SW. Genome Biol.
2007;8(8):R173.
Cloning and expression of new microRNAs from zebrafish. W. P. Kloosterman, F. A. Steiner, E.
Berezikov, E. de Bruijn, J. van de Belt, M. Verheul, E. Cuppen, and R. H.A. Plasterk. Nucleic Acids
Res. 34, 2558-2569 (2006).
Differences in vertebrate microRNA expression. Ason B, Darnell DK, Wittbrodt B, Berezikov E,
Kloosterman WP, Wittbrodt J, Antin PB, Plasterk RH. Proc Natl Acad Sci U S A. 2006 Sep
26;103(39):14385-9.
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes.
Kloosterman WP, Wienholds E, de Bruijn E, Kauppinen S, Plasterk RH. Nat Methods. 2006
Jan;3(1):27-9.
MicroRNA expression during chick embryo development. Darnell DK, Kaur S, Stanislaw S, Konieczka
JH, Yatskievych TA, Antin PB. Dev Dyn. 2006 Nov;235(11):3156-65.
FGF-4 signaling is involved in mir-206 expression in developing somites of chicken embryos.
Sweetman D, Rathjen T, Jefferson M, Wheeler G, Smith TG, Wheeler GN, Münsterberg A, Dalmay T.
Dev Dyn. 2006 Aug;235(8):2185-91.
MicroRNA Expression in Zebrafish Embryonic Development. Erno Wienholds, Wigard P.
Kloosterman, Eric Miska, Ezequiel Alvarez-Saavedra, Eugene Berezikov, Ewart de Bruijn, Robert H.
Horvitz, Sakari Kauppinen, and Ronald H. A. Plasterk. Science 2005 Jul 08;309(5732):310-311.
Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles
during larval growth . Sokol, Nicholas S. · Ambros, Victor. Genes & Development, 19 (19), 2343 2354 (2005).
LNA™ in situ hybridization (and Northern blotting) - PLANTS
Spatio-temporal accumulation of microRNAs is highly coordinated in developing plant tissues. Válóczi
A, Várallyay E, Kauppinen S, Burgyán J, Havelda Z. Plant J. 2006 Jul;47(1):140-51.
Redundancy and specialization among plant microRNAs: role of the MIR164 family in developmental
robustness. Sieber P, Wellmer F, Gheyselinck J, Riechmann JL, Meyerowitz EM. Development. 2007
Mar;134(6):1051-60.
MicroRNA-mediated regulation of stomatal development in Arabidopsis. Kutter C, Schöb H, Stadler
M, Meins F Jr, Si-Ammour A. Plant Cell. 2007 Aug;19(8):2417-29.
LNA™ in situ hybridization and Northern blotting
Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumors. Lee EJ,
Baek M, Gusev Y, Brackett DJ, Nuovo GJ, Schmittgen TD. RNA. 2008 Jan;14(1):35-42.
Post-transcriptional regulation of the let-7 microRNA during neural cell specification. Wulczyn FG,
Smirnova L, Rybak A, Brandt C, Kwidzinski E, Ninnemann O, Strehle M, Seiler A, Schumacher S,
Nitsch R. FASEB J. 2007 Feb;21(2):415-26.
Post-transcriptional regulation of microRNA expression. Obernosterer G, Leuschner PJ, Alenius M,
Martinez J. RNA. 2006 Jul;12(7):1161-7.
LNA™ in situ hybridization and Northern blotting after LNA™-AntimiR use
Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to upregulation of a large set of predicted target mRNAs in the liver. Elmén J, Lindow M, Silahtaroglu A,
Bak M, Christensen M, Lind-Thomsen A, Hedtjärn M, Hansen JB, Hansen HF, Straarup EM,
McCullagh K, Kearney P, Kauppinen S. Nucleic Acids Res. 2008 Mar;36(4):1153-62.