NZQA registered unit standard
12363 version 4
Page 1 of 4
Title
Demonstrate knowledge of chromatography systems
Level
5
Credits
4
Purpose
People credited with this unit standard are able to: describe
chromatography, the distribution between phases in a
chromatographic separation, and the instrumental components
of a chromatographic system; quantify the components of a
mixture from chromatographic results; and recommend a
chromatographic system for a given sample.
Classification
Science > Chemistry
Available grade
Achieved
Explanatory notes
1
All work must be carried out in accordance with the quality management system,
documented protocol system or Standard Operating Procedures typically acceptable
in a commercial or research laboratory.
2
Health and Safety practices must conform to Australian/New Zealand Standard
AS/NZS 2243:2006 Set – Safety in Laboratories Parts 1, 2, 3, 7, and 10 available at
http://www.standards.co.nz and http://infostore.saiglobal.com/store.
3
Legislation applicable to this unit standard includes:
Health and Safety in Employment Act 1992;
Hazardous Substances and New Organisms Act 1996.
4
The chromatography systems covered by this unit standard are: Gas Liquid
Chromatography (GLC); High Pressure Liquid Chromatography (HPLC); and Ion
Chromatography.
Outcomes and evidence requirements
Outcome 1
Describe chromatography.
Evidence requirements
1.1
Chromatography is described in terms of separation using mobile and stationary
phases.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2016
NZQA registered unit standard
1.2
12363 version 4
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Chromatography is described in terms of workable phase combinations.
Range
evidence of two examples of workable phase combinations is
required.
Outcome 2
Describe the distribution between phases in a chromatographic separation.
Evidence requirements
2.1
The distribution is described in terms of the system characteristics affecting the
separation.
2.2
The distribution is described in terms of the sample characteristics.
2.3
Examples of sample component are given, and the order of elution of the
sample component is predicted in accordance with the chromatographic
procedure.
Range
evidence of two examples of elution component is required.
Outcome 3
Describe the instrumental components of a chromatographic system.
Evidence requirements
3.1
The mobile phase delivery system is described in terms of its composition and
requirements.
3.2
The sample delivery system is described.
Range
3.3
The column characteristics are described.
Range
3.4
septum, heated port, valve and loop;
HPLC – valve and loop.
column dimensions, stationary phase, support.
The detection system is described.
Range
thermal conductivity detector (TCD) and flame ionisation detector
(FID); ultra violet (UV) and refractive index (RI);
for Ion Chromatography – conductivity.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2016
NZQA registered unit standard
12363 version 4
Page 3 of 4
Outcome 4
Quantify the components of a mixture from chromatographic data results.
Range
external calibration curve, internal standard calculation where sample and
standards contain equal amounts of internal standard.
Evidence requirements
4.1
Components are quantified consistent with the data results.
Outcome 5
Recommend a chromatographic system for a given sample.
Range
sample may include – volatile and non-volatile organics, inorganic gases,
aqueous-based solutions.
Evidence requirements
5.1
The chromatographic system is selected and recommended in accordance with
the sample characteristics.
Planned review date
31 December 2017
Status information and last date for assessment for superseded versions
Process
Version Date
Last Date for Assessment
Registration
1
17 February 1998
31 December 2014
Review
2
23 November 1999
31 December 2014
Review
3
18 June 2010
N/A
Rollover
4
27 January 2015
N/A
Consent and Moderation Requirements (CMR) reference
0152
This CMR can be accessed at http://www.nzqa.govt.nz/framework/search/index.do.
Please note
Providers must be granted consent to assess against standards (accredited) by NZQA,
before they can report credits from assessment against unit standards or deliver courses
of study leading to that assessment.
Industry Training Organisations must be granted consent to assess against standards by
NZQA before they can register credits from assessment against unit standards.
Providers and Industry Training Organisations, which have been granted consent and
which are assessing against unit standards must engage with the moderation system that
applies to those standards.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2016
NZQA registered unit standard
12363 version 4
Page 4 of 4
Requirements for consent to assess and an outline of the moderation system that applies
to this standard are outlined in the Consent and Moderation Requirements (CMR). The
CMR also includes useful information about special requirements for organisations wishing
to develop education and training programmes, such as minimum qualifications for tutors
and assessors, and special resource requirements.
Comments on this unit standard
Please contact NZQA National Qualifications Services nqs@nzqa.govt.nz if you wish to
suggest changes to the content of this unit standard.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2016