NZQA registered unit standard
8065 version 4
Page 1 of 3
Title
Perform a restriction enzyme digestion
Level
6
Credits
3
Purpose
People credited with this unit standard are able to: discuss the
use of restriction enzymes; carry out restriction enzyme digest;
and interpret results.
Classification
Science > Molecular Biology
Available grade
Achieved
Entry information
Recommended skills
and knowledge
Unit 8050, Perform electrophoresis; and Unit 26488, Determine
enzyme activity; or demonstrate equivalent knowledge and
skills.
Explanatory notes
1
All work must be carried out in accordance with the quality management system,
documented protocol system or Standard Operating Procedures (SOP) acceptable in
a commercial or research laboratory.
2
Health and Safety practices must conform to Australian/New Zealand Standard
AS/NZS 2243:2006 Set – Safety in Laboratories Parts 1, 2, 3, 7 and 10 available at
http://www.standards.co.nz and http://infostore.saiglobal.com/store.
3
Legislation applicable to this unit standard includes:
Health and Safety in Employment Act 1992;
Hazardous Substances and New Organisms Act 1996.
4
Glossary
Laboratory procedures refer to documented systems or processes of operation which
may be found in a SOP manual, quality management system, or in protocol system
documentation. These procedures are external and/or internal laboratory
requirements governing laboratory work.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2016
NZQA registered unit standard
8065 version 4
Page 2 of 3
Outcomes and evidence requirements
Outcome 1
Discuss the use of restriction enzymes.
Evidence requirements
1.1
Restriction enzymes are discussed in terms of their mode of action.
Range
1.2
5' ’sticky ends’, 3' ’sticky ends’, blunt ends, target sequences.
Restriction enzyme digestions are discussed in terms of their application.
Range
restriction fragment length, restriction fragment length
polymorphism analysis (RFLP), DNA profiling.
Outcome 2
Carry out restriction enzyme digest.
Evidence requirements
2.1
Enzyme is handled to ensure activation in accordance with laboratory
procedures.
2.2
Fragments are produced and visualised by electrophoresis in accordance with
laboratory procedures.
2.3
Separation of fragments is achieved to allow fragments to be identified.
Outcome 3
Interpret results.
Evidence requirements
3.1
Results are recorded and analysed in accordance with laboratory procedures.
3.2
Interpretation is consistent with results and sample.
Planned review date
31 December 2018
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2016
NZQA registered unit standard
8065 version 4
Page 3 of 3
Status information and last date for assessment for superseded versions
Process
Version Date
Last Date for Assessment
Registration
1
22 December 1996
31 December 2014
Review
2
23 November 1999
31 December 2014
Review
3
17 September 2010
N/A
Rollover
4
27 January 2015
N/A
Consent and Moderation Requirements (CMR) reference
0152
This CMR can be accessed at http://www.nzqa.govt.nz/framework/search/index.do.
Please note
Providers must be granted consent to assess against standards (accredited) by NZQA,
before they can report credits from assessment against unit standards or deliver courses
of study leading to that assessment.
Industry Training Organisations must be granted consent to assess against standards by
NZQA before they can register credits from assessment against unit standards.
Providers and Industry Training Organisations, which have been granted consent and
which are assessing against unit standards must engage with the moderation system that
applies to those standards.
Requirements for consent to assess and an outline of the moderation system that applies
to this standard are outlined in the Consent and Moderation Requirements (CMR). The
CMR also includes useful information about special requirements for organisations wishing
to develop education and training programmes, such as minimum qualifications for tutors
and assessors, and special resource requirements.
Comments on this unit standard
Please contact NZQA National Qualifications Services nqs@nzqa.govt.nz if you wish to
suggest changes to the content of this unit standard.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2016