OCEAN COUNTY COLLEGE Professor’s Syllabus Professor’s Name: Dr. Catherine Murphy Course Title and Number: General Microbiology/BIOL 264 Lab (Should be taken concurrently with BIOL 264 Lecture) Catalog Description: This course is a study of the various groups of microorganisms, their morphology, physiology, biochemistry, genetics and importance to man and all other life forms. The course will emphasize the major microbial model systems, laboratory techniques of growth, control, research utilization and safety. Current DNA recombinant technology, immunology and biotechnology will also be included. Semester: Fall 2012 (September 5 – December 21) Office Location: Room H232 Phone: (732) 255-0400 extension 2263 Email: cmurphy@ocean.edu or catherine_murphy@portal.occ.edu Office hours: Monday, 10:55-11:50, Tuesday, 8:30-9:20 & 10:55-11:20, Wednesday, 9:25-9:50, Thursday, 10:55-11:50, Friday, 10:55-11:50 Other Meeting Times: To arrange a meeting with your instructor in addition to the regularly scheduled office hours, please contact your instructor directly or contact the Department of Science (extension 2178/khamilton@ocean.edu). Required Text and other Materials: Tortora, G.J., B.R.Funke and C.L. Case. 2010. Microbiology: An Introduction, 10th Edition.. Lab Manual: Available in Ocean Cruiser under Shared files. Course Learning Outcomes/Objectives: The course is intended primarily to serve the needs of students of science and related fields. As a basic science, Microbiology will be highly laboratory oriented and aimed at developing understandings and techniques of investigation. The study will include the scientific principles of the morphology and physiology of microorganisms, their impact on all aspects of life and survival on the planet. Microbes involved in medicine will be reviewed. Their use as research tools will also be explored. The new frontiers of genetic engineering, recombinant DNA, immunology and biotechnology will also be included. General Education Goals Addressed in the Course: 1. To develop the ability to become an independent thinker through mathematical, scientific and philosophical reasoning. 2. To develop the ability to solve problems by collecting, organizing and evaluating information. 3. To develop an understanding of the concepts, theories and fundamental principles of the natural and social sciences. 4. To develop a global perspective on problems and issues that humankind faces, and to explore solutions which are morally, socially, economically, politically and ecologically sound. Course Standards: No collaboration is allowed on any exam. Cheating on exams or plagiarism on reports will be dealt with according to College policies. Refer to Student Handbook Policy # 5180. Cell phones must be turned off and put away during exams and quizzes. Cell phones must be turned off and put away during the lab period. Cells phones must be put either vibrate or silent during lecture. Ringing cell phones and texting disrupts the class and is not allowed. You may tape the lectures. Always feel free to raise your hand to ask questions. In the event that you should need to withdraw from this course please do so by the appropriate time allowed by the Ocean County College calendar. If a student discontinues attending class and does not properly withdraw, an F will be given for a final grade. Attendance Policy: Attendance at all lectures and labs is expected. All absences must be granted by an individual faculty member, who will make the final decision on whether or not to accept a student’s excuse for planning to miss class(es) or for having missed class(es). In emergency situations such as a death in the family or serious illness or other unforeseen occurrence, a student who does not have time to notify his or her Professor should contact the departmental secretary, ext. 2178. A grade of “F” will be assigned to any student who stops attending and does not officially withdraw from the course. Grading Scale: A = B+ = B = C+ = C = D = F = 90-100 86-89 80-85 76-79 70-75 60-69 < 60 Course Outline: Lecture Schedule: Topic The Microbial World and You Functional Anatomy of Prokaryotic and Eukaryotic Cells Quiz 1 Microbial Metabolism Exam 1 Microbial Growth The Control of Microbial Growth Quiz 2 The Eukaryotes Exam 2 Viruses, Viroids, and Prions Principles of Disease and Epidemiology Quiz 3 Microbial Mechanisms of Pathogenicity Innate Immunity Exam 3 Antimicrobial Drugs Microbial Genetics Biotechnology and Recombinant DNA Final Exam Chapter in Text Chapter 1 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 12 Chapter 13 Chapter 14 Chapter 15 Chapter 16 Chapter 20 Chapter 8 Chapter 9 BIOL 264 Laboratory Schedule: Date Topic 9/07/12 Orientation/Safety; Microscopy – Eukaryotic Microbes & Bacteria 9/14/12 [LAB Worksheet 1 from Lab Manual, page 9 - DUE]; Smear Preparation & Simple Stain 9/21/12 Introduction to Unknown: Aseptic Technique – Inoculate streak plate, broth and slant using Unknown 9/28/12 Cultural Characteristics – Analysis of Broth, Slant & Plate; Motility - Wet Mount prepared from Broth culture 10/05/12 Differential Staining - Gram Stain 10/12/12 Metabolism – Biochemical Test Media; Differential Staining Spore Stain 10/19/12 Metabolism (Assessment); Microbe Sensitivity Test – Inoculation (Disk Diffusion plates) 10/26/12 Microbe Sensitivity Test – Evaluation 11/02/12 Conjugation Experiment – Media preparation; [PET LAB REPORT DUE] 11/09/12 Conjugation Experiment (Part I & Part II); [LAB Worksheet 2 from Lab Manual, page 38 and 42 - DUE] 11/16/12 Conjugation Experiment (Part III) [LAB Worksheet 4 from Lab Manual, page 55 -DUE] 11/23/12 Thanksgiving Break 11/30/12 Enumeration of Microbes in Food - Serial Dilution and Pour Plate 12/07/12 Enumeration of Microbes in Food –Assessment, [Lab Worksheet 3 from Lab Manual, page 47 - DUE] 12/14/12 Lab Final 12/21/12 Lab Final Review Statement on Plagiarism: Students should refer to the Student Handbook and review Policy #5180. Statement about Civility: Ocean County College defines civility primarily as the demonstration of respect for others, basic courtesy, reciprocity (treating others as we wish to be treated), and behaviors that create a positive environment in which to learn and to work. Lecture begins promptly. As a courtesy to your fellow students and your Professor, please arrive on time. Questions and participation in lecture discussions is strongly encouraged. Distracting “side bar” conversations will not be tolerated. Cell phones must be turned off or placed on vibrate setting during the lab and lecture periods. Evaluation of Students: 60% Exams (4 exams, 15% each) 15% Lab Final (Cumulative) 10% Quizzes (3 quizzes, lowest dropped) 15% Lab Reports and worksheets (1 Formal lab report, 4 worksheets) Exam format: The types of questions on exams will include multiple choice, matching true or false, fill in, and short answer. *If a student is absent on an exam day, the student may make up the exam at the instructor's convenience. Make up exams will be ESSAY type. Quizzes can not be made up, therefore absence from a quiz is a grade of “0”. It is your responsibility to read the lab exercises prior to the lab. You will be receiving minimal instruction on how to perform the lab experiments, so it is vital that you come prepared. There are no make-up labs. If you are absent for a lab exercise, you are still responsible for the material covered. If you are absent from lab more than two times, points will be deducted from your lab grade. Five (5) points will be subtracted from the final lab grade for each lab that is missed, with the exception of the two allowed absences. Formal Lab reports must be written in a specific format as indicated in the Lab Manual found in Ocean Cruiser Shared files. Lab reports and Worksheets are due according to the schedule above. Campus Resources and Services: There is tutoring available (a) in the writing lab for all subject areas, not just English courses, and (b) in the Mathematics Tutoring Center. In addition Student Success Seminars are scheduled each week and are posted on the college website under “Campus Resources and Services.” More information on college services can be found by using the A-Z index on the college website (for example, under “T’ for Tutoring or under “S” for Study Strategy Sessions.) Statement of Accommodations: If there is any student in this class who has special needs because of learning disabilities or other kinds of disabilities, please feel free to come and discuss this with me, or a staff member in the Center for Academic Excellence. Disclaimer: Individual faculty members may make reasonable changes to his course outline exclusive of course requirements, course calendar, and grading procedures. All individuals should not assume that anything received, sent, or stored in this course or in any course is private. Students’ written work, assignments, and test results may be used anonymously for college assessment purposes. Course content, support materials, and communications (including chats, discussions, emails, and any other forms of communication) may be used for quality assurance purposes by authorized college administrators. Important Notes Ocean Cruiser is the official email communication for students at OCC (firstname_lastname@occ.mailcruiser.com) Failure to pay for this course may result in your being dropped for non-payment.