August 2006 CURRICULUM VITAE Paul Anthony Insel, M.D. Departments of Pharmacology and Medicine University of California, San Diego 9500 Gilman Drive La Jolla, California 92093-0636 858/534-2295 (Work) 858/822-1007 (Fax) pinsel@ucsd.edu (E-Mail) Residence: 8660 Kilbourn Drive La Jolla, California 92037 858/558-1816 (Phone/Fax); 858/472-1817 (Cell) Birth date: November 22, 1945 – New York, NY Education: 1962-64 1964-68 George Washington University, Washington D.C. University of Michigan, Ann Arbor, Michigan, M.D., cum laude Military Service: 1970-74 United States Public Health Service (NIH) Professional Experience: 1968-69 1969-70 Honors and Awards 1965 1966-68 1966 1968 1976 1977-82 1981 1994 2002 2005 Rollo McCotter Award, University of Michigan Dean's Award, University of Michigan Alpha Omega Alpha, University of Michigan Lange Award, University of Michigan Katz Research Prize, American Heart Association (runner-up) Established Investigatorship, American Heart Association U.S.-France Cooperative Program in Cancer Research and INSERM Visiting Fellowship UCSD School of Medicine Faculty Distinguished Lecturer Honorary Doctorate (Doctorate Honoris Causa), University of Paris–XII American Association for the Advancement of Science Fellow Board Certifications 1969 1970 1973 National Board of Medical Examiners, Diplomate Medical License in California, Maryland American Board of Internal Medicine, Diplomate Professional 1969 1976 1978 1978 1979 American Association for the Advancement of Science American Federation for Clinical Research Western Society for Clinical Investigation American Society for Pharmacology and Experimental Therapeutics American Society for Cell Biology Intern, Harvard Medical Unit, Boston City Hospital, Boston, Mass. Asst. Resident, Harvard Medical Unit, Boston City Hospital, Boston, Massachusetts; Clinical Fellow in Medicine, Harvard Medical School 1970-74 Clinical Associate and Medical Officer, National Institute of Child Health & Human Development, Gerontology Research Center, NIH, Baltimore, MD 1970-72 Attending Physician, Endocrine-Metabolic Clinic, Baltimore City Hospitals, Baltimore, Maryland 1971-74 Assistant in Medicine, Dept. of Medicine, Johns Hopkins University School of Medicine 1974-77 Research Fellow, Div. of Clinical Pharmacology, Dept. of Medicine and Cardiovascular Research Institute, Univ. of Calif. San Francisco 1974-78 Attending Physician, Emerg. Service, San Francisco General Hospital 1977-78 Assistant Professor of Medicine-in-Residence and Associate, Cardiovascular Research Institute, University of California, San Francisco 1978-81 Assistant Professor of Medicine, Division of Pharmacology, UCSD. 1978Member, Cancer Center, Biomedical Sciences Graduate Program, Neurosciences Graduate Program, Molecular Pathology Graduate Program, UCSD. 1981-87 Associate Professor of Medicine, Division of Pharmacology, UCSD 1981 & 86 Visiting Scientist, INSERM Unite 99 Creteil, France 1987Professor of Pharmacology and Professor of Medicine, UCSD. 1989Director, M.D.-Ph.D. (Medical Scientist Training) Program, UCSD. 2005 Consultant/Visiting Scientist, NHLBI, NIH. Page 1 1979 1980 1980 1980 1983 1997 Endocrine Society American Heart Association (Basic Science & Hypertension Councils) American Society for Clinical Investigation American Society for Biochem. and Mol. Biol. Council for High Blood Pressure Research Association of American Physicians Editorial Boards: 1978-92 19801983-05 1988-91 1988-93 1988-94 1990-95 19901993-95 1994-00 1999-00 20002000200120022003200420052005- Annual Review of Pharmacology and Toxicology Journal of Receptors and Signal Transduction Res. (formerly J. Cyclic Nucleotide Res.) Molecular Pharmacology Endocrinology Journal of Biological Chemistry American Journal of Physiology (Renal Fluid and Electrolyte Physiology) Hypertension American Journal of Physiology (Cell Physiology) Pharmacology and Toxicology Journal of Molecular Medicine Prevention Magazine (Advisory Board) Nauyn Schmiedeberg's Archives of Pharmacology American Journal of PharmacoGenomics Biomed Central Pharmacology (Subject Advisor) Pharmacogenetics Journal of American College of Cardiology (Editorial Advisor) Purinergic Signaling FASEB Journal Molecular Diagnosis & Therapy Editorships: 1992-97 1992-93 19961998-01 1998-01 19992001-03 20002003-06 2006- Deputy Editor, Journal of Clinical Investigation; Co-Chief Editor 1996-97 Associate Editor, Circulation Associate Editor, American Journal of Physiology-Cell Physiology Associate Editor, Journal of Pharmacology and Experimental Therapeutics Associate Editor, British Journal of Pharmacology Associate Editor, Annual Review of Pharmacology and Toxicology Associate Editor, Molecular Pharmacology Associate Editor, Pharmacological Reviews Editor-in-Chief, Molecular Pharmacology Co-Chief Editor, Faculty of 1000, Pharmacology Visiting Professorships and Invited Seminars (since 1985) National Institutes of Health, NIGMS/PRAT Program; NIA; NICHD University of Washington, Dept. of Pharmacology University of Iowa, Dept. of Medicine UCLA, Dept. of Pharmacology University of California, Davis, Dept. of Biochemistry University of California, Riverside, Division of Biomedical Sciences St. Louis University, Dept. of Biochemistry University of Southern California, Dept. of Physiology University of Southern Calif., Dept. of Biochemistry University of Michigan, Dept. of Pharmacology Bowman Gray School of Medicine University of California, Irvine, Dept. of Pharmacology Abbott Laboratories, Pfizer, Allergan, Easai Pharmaceutical Corporation, Wyeth-Ayerst, Proctor and Gamble Research Laboratory, Genentech, Specialty Laboratories, Syntex, Bristol-Myers Squibb Res. Institute, Lederle Laboratories, Athena Neurosciences, Small Molecule Therapeutics, Isis Pharmaceuticals, Axiom Biotechnologies, Acadia Pharmaceuticals; Theravance University of Leicester, Dept. of Pharmacology Glasgow University, Dept. of Biochemistry University of London, Dept. of Biochemical Pharmacology Mario Negri Institute, Italy University of North Carolina, Dept. of Biochemistry City University of Yokohama, Dept. of Pharmacology University of Tokyo, School of Pharmaceutical Chemistry University of Tubingen, Institute of Pharmacology and Toxicology Page 2 University of South Carolina, Depts of Pharmacology and Medicine Emory University Case Western Reserve University, Dept. of Pharmacology Cleveland Clinic Foundation Scripps Clinic and Research Foundation INSERM U-99 University of Essen (Germany), Dept. of Pharmacology; Depts. of Medicine & Pediatrics Stanford University, Howard Hughes Medical Institute Endocrine Society, Introduction to Endocrine Research; Introduction to Molecular & Cellular Research Washington University, Myocardial SCOR Program Georgetown University, Dept. of Physiology and Dept. of Pharmacology University of Western Ontario, Dept. of Pharmacology, London Cardiovascular Research Society Brown University, Depts. of Medicine and Pharmacology Dartmouth University, Dept. of Pharmacology Oberlin College, Depts. of Biology and Neurosciences University of South Alabama, Dept. of Pharmacology Stanford University, Hopkins Marine Station Columbia University, Dept. of Pharmacology Thomas Jefferson University, Dept. of Physiology SUNY Stonybrook, Undergraduate Colloquium University of Nevada, Reno, Dept. of Pharmacology Loma Linda University, Perinatal Biology Division University of Texas, San Antonio, Dept. of Pharmacology University of Pennsylvania, Dept. of Pharmacology University of Minnesota, Dept. of Pharmacology University of Houston School of Pharmacy Harvard Medical School, Seminar in Medicine, Beth Israel Hospital Harvard-University of Massachusetts Toxicology Research Roundtable Presidential Lecturer, American College of Neuropsychopharmacology State of the Art Lecturer, Western Clinical Research Meetings Cornell University Medical School, Dept. of Pharmacology University of Pittsburgh, Dept. of Pharmacology Scripps Research Institute University of Colorado, Dept. of Pharmacology Hokkaido Univ., Dept. of Pathology and Cardiovascular Medicine (Japan) Baylor Univ., Dept. of Medicine New York Univ., Dept. of Medicine University of Alabama, Dept. of Physiology University of Utah, Dept. of Pharmacology Karolinska Inst.(Sweden) Dalhousie University, (Peter Dresel Memorial Lecturer) University of South Alabama (Distinguished Basic Science Lecturer) University of Virginia (Medical Student Research Symposium, Keynote Address) Professional Service: Consultant & 1983 1982-86 1982-87 1978- 19781987-93 1990-95 1991 1991-98 1992-94 1994-00 1997-00 1993 Member, Adv. Panel on Cell. and Molec. Neurobiology; Grant Reviewer, NIMH Member, Pharmacology Study Section, NIH Member, Research Allocation Committee (Chair, 1984-1985), San Diego Heart Assn. NSF, Veterans' Administration, March of Dimes Fdn, Canadian Med. Research Council, Australian Med. Research Council, NIH Ad Hoc reviewer, Wellcome Trust Medical Research Council of Hong Kong; Medical Res. Council UK Ad-hoc reviewer American Cancer Soc. and NIH (NIGMS, NHLBI, NIA, NINCDS, NCI, NINCDS, NCI, DRG) Ad Hoc study section member American Heart Association, California Affiliate South Peer Review Committee, Vice Chair (1989-1991); Chair (1991-1993) Amer. Heart Assoc., Western States Affiliate Research Committee; Vice-chair (1997-99) Board of Science. Councilors (Ad Hoc), Natl. Inst. on Aging Member, Pharmacology Test Cmte, Natl. Board of Medical Examiners; Chair (1994-98) Chair, Applied Pharmacology Task Force, Natl Board of Medical Examiners Member, National Board of Medical Examiners Chair, Step 1 Committee, National Board of Medical Examiners Board of Scientific Advisors, Sandoz Research Institute Page 3 1993 19951996 1996-02 1997 1997 1997 1997,2000 1998-03 19991999-03 2000-01 2000-02 2002-03 20032002-03 2003-04 2002-06 2004 2004 2006 2006-09 Member, Medical Research Council (UK) Review of Cell Regulation Board of Scientific Councilors (Ad Hoc), NIMH, NINDS, NICHD NIGMS Advisory Council, Ad Hoc Member Scientific Advisory Board, Collateral Therapeutics IUPHAR Nomenclature Committee on P2-purinergic receptors University of South Carolina, NSF EPSCO Advisory Committee University of Illinois, M.D./Ph.D. Program Advisory Committee External Advisor, Case Western Reserve University, Pharmacology Training Grant Member, Graduate Research and Training Group, American Association of Medical Colleges; Nominating Committee Member, Program Committee Member American Association of Medical Colleges (AAMC), Advisory Panel on Research; Medical School Curriculum Review Committee Member, Interdisciplinary Committees, National Board of Medical Examiners American Association of Medical Colleges, Basic Science Content Panel Scientific Advisory Board, Axiom Biotechnologies Scientific Advisory Board, Prime Biotech Scientific Advisory Board, Sequenom External Advisor, Cell and Molecular Biology Grad Program, Univ. Pennsylvania and Medical Scientist Training Program, Yale University External Examiner, PhD Theses: Karolinska Institute, Sweden; Nat. Univ Singapore; U of Houston Chair, Keystone Symposium on G-Protein-Coupled Receptors Scientific Advisory Board Member, Cambridge Health Tech Institute Signal Transduction Conference Advisory Member, NIH Roadmap Workshop on Clinical Research Training Ad-hoc Reviewer, Howard Hughes Medical Institute Council Delegate, Medical Sciences, American Association for the Advancement of Science Manuscript Reviewer: Science; Proceedings Natl. Acad. Science, USA; Analytical Biochemistry; Biochemistry; (since 1985) Life Sciences; Biochemical Pharmacology; Journal of Pharmacology and Experimental Therapeutics; FEBS Letters; Journal of Clinical Investigation; Cancer Research; FASEB Journal/Federation Proceedings; Hypertension; Circulation Research; American Journal of Physiology; Molecular and Cellular Endocrinology; Journal of Gerontology; Metabolism; Journal of Allergy & Clinical Immunology; Circulation; Kidney International; Blood; Archives Biochemistry and Biophysics; Journal of Neuroscience; New England Journal of Medicine; Biochimica Biophysica Acta; Journal of Cell Biology; Journal of Molecular and Cellular Cardiology; Journal of Lipid Research; Proceedings of Society for Experimental Biology and Medicine; Association of Proceedings of American Physicians; Regulatory Peptides; European Journal of Pharmacology; Journal of Hypertension; Cell Regulation; Journal of Cell Physiology; Molecular Endocrinology; Trends in Cardiovascular Sciences; Human Genetics, Pharmacogenetics; J Cardiac Failure ; Trends in Pharmacological Sciences, Journal of American College of Cardiology Professional Organizations Councilor, Western Society for Clinical Investigation Publications Committee, Council for High Blood Pressure Research, Am. Heart Assn. President, Pharmacology Education and Research Foundation Program Committee, Am. Soc. Pharmacol. Exp. Therapeutics (Chair, 1996-1999) John Abel Award Selection Committee, Am. Soc. Pharmacol. Exp. Therap, Chair (1993) Program Theme Committee Member Experimental Biology; Chair, Signal Transduction Theme Nominating Committee, Am. Soc. Pharmacol. Exp. Therap. ASPET Award Selection Committee Program Committee, Experimental Biology: '98; Experimental Biology '99; Intl Pharmacol. IUPHAR Meeting, 2002 President, National Association of MD-PhD Directors; 1997- Member, Executive Committee ASPET representative to Experimental Biology Board Chair, Experimental Biology Board 1980-83 1988-91 19901992-99 1992-93 1994-96 1995 1995 1997 1997-00 19992000- University Service: Univ. of Calif. Systemwide: Member, Cancer Research Coord. Committee, 1982-1985; Chair, 1984-85 Page 4 UCSD Clinical Research Center Advisory Committee (1985-1986; Chair 1987-1988); Board of Inquiry, VA Medical Center UCSD: Distinguished Faculty Lecture Committee (1987, 1995); American Cancer Society Award Committee (1988); Searle Award Selection Committee (1990); Ad Hoc Promotion Committees (1992-), Pharmacy School Dean Search Committee; Chair, 2000-2002 School of Medicine Academic Senate Research Committee (1987-1990; Chair, 1988-1990) UCSD School of Medicine: Nominating Committee (1987-1990); Chair 1988;1994-96; Chair, 199798); Ad Hoc Member (1998-99); Recruitment and Admissions Committee (1989-1991, 1992); Faculty Council (Vice Chair, 1990-1991; Chair, 1991-1992, Past Chair 1992-93; Member 1997); Student Summer Research Steering Committee (1994-97); Graduation Speaker Selection Committee (1995-99); Dean’s Self Study Review Committee (1995-96); 25th Anniversary Outstanding Faculty (1998) Selection Committee (1997); Strategic Planning Committee (199798); Strategic Planning Transition Team Committee (1998); Graduate Program Support Committee, Chair (2000); Graduate Program Education Committee (2001-); Self Study Review Committee in Graduate Training (2002); Department of Pediatrics, Search Committee (2004) UCSD Biomedical Sciences (formerly Physiology-Pharmacology) Graduate Program: Core Committee Member; Steering Committee member; Graduate Student Advisor; Admissions Committee member; Minor Proposition Committee member and Chair; Major Proposition Committee member and Chair; Standing and Promotions Committee Chairman; Faculty Membership Committee member UCSD Neuroscience Graduate Program: Minor Proposition Committee UCSD Biology Dept.: Graduate Thesis and Minor Prop. Comm. Member UCSD Chemistry Dept.: Minor Proposition and Graduate Thesis Committee member UCSD Medical Student Advisor UCSD Dept. of Medicine: several ad hoc, review and search committees; Advisory Committee (198386); Education Committee (1984-87; 1999-); Research Committee (1986-88); Honors and Awards Committee (1988-91) UCSD Dept. of Pharmacology: Chair and Member, several ad/hoc review and search committees; Joint Appointments Committee (Chair); Space Committee; Teaching Committee; Long Range Planning Committee UCSD School of Pharmacy Planning Committee Member (2000-2); Dean's Search Committee, Chair (2001); Educational Policy and Academic Oversight Committee (2002-); Committee on Academic Personnel (2004-) Stein Research Institute Scientific Advisory Board (2004-) Research Support: 1977-82 Established Investigator, Amer. Heart Assoc., "Adrenergic Receptors and Cyclic AMP in Cellular Function" – $128,000 1977-78 UCSF Academic Senate Committee on Research and Research Evaluation and Allocation Committee, "Identification and Regulation of Beta-adrenergic Receptors of Cultured S49 Cells – $5,500 1977-78 National Science Foundation, "Genetic Analysis of Hormone Action and Cell Growth" (CoP.I. with Henry Bourne) – $70,000 1978-79 NIH, "Hormonal Control of Uterine Adrenotropic Receptors" (co-invest. with Dr. James Roberts and Alan Goldfien) – $96,000 1978-79 UCSF Committee on Research Evaluation and Allocation, "Insulin Action in Cultured S49 Cells" – $3,000 1978-80 UCSD Academic Senate Committee on Research, "Cellular Mechanism of Refractoriness to Catecholamines" – $4,500 1979-82 National Science Foundation, "Regulation of Beta-adrenergic Receptors and Response" – $211,000 1979-82 American Heart Association "Molecular Properties of -adrenergic Receptors" – $69,000 1979-81 California Heart Association, "Regulation of Beta-adrenoreceptors by Glucocorticoids" – $17,477 1979-80 American Cancer Society, Institutional Research Grant "Polyamine Biosynthesis in S49 Lymphoma Cells" – $2,500 1980-82 Calif. Heart Assoc., "Adrenergic Receptors in Hypertension" – $16,209 1980-85 NIH, SCOR in Hypertension (PI, Morton Printz) Unit 4, "Adrenergic Receptors in Hypertension" and Core, "Radioiodination and Radioligand Binding", – $86,800 1982-83 University of California Cancer Research Coordinating Committee, "Cell Cycle Analysis of Lymphoma Cells Grown in Defined Medium" – $15,500 1982-84 American Cancer Society, "Function of Tumor Cells Grown in Defined Medium" - $121,000 Page 5 1982-85 National Science Foundation, "Regulation of -adrenergic Receptors and Response" – $210,000 1983-85 NIH, "Cellular Metabolism of Adrenergic Receptors" – $217,000 1985-88 National Science Foundation, "Regulation of -adrenergic Receptors and Response" – $290,000 1985-86 Elsa U. Pardee Foundation, "Catecholamine Receptors in Human Erythroleukemia Cells" – $29,000 1986-91 NIH, "Cellular Metabolism of Adrenergic Receptors" – $562,000 1986-91 NIH, "Renal Adrenergic Receptors in Hypertension" – $542,400 1988-93 NIH, "-Adrenergic Receptors in S49 Lymphoma Cells"– $548,000 1989-90 Univ. of California, “Generation of phospholipase C variants and oligonucleotide inhibitors of protein kinase C” - $31,000 1990-95 NIH, SCOR in Myocardial Ischemia (PI, John Ross), Unit I, “Adrenergic Receptors and Post-Receptor Components in Myocardial Ischemia” – $117,000 1991-95 NIH, "Cellular Action of Adrenergic Receptors" – $414,000 1992-97 NIH, "Genetic and receptor mechanisms in hypertension" (PI, Morton Printz), Unit 4, Renal adrenergic receptors in hypertension, – $540,008 1993-97 NIH, "-adrenergic receptors in S49 lymphoma cells" – $497,300 1995-00 NIH, SCOR in Heart Failure (PI, John Ross), Unit III, “Adrenergic receptor signalling in heart failure”, $115,000 1995-99 NIH "Cellular Action of Adrenergic Receptors" – $580,000 1999-04 NIH, "Sympathetic Neuroeffector Junctions and Blood Pressure" (PI, D.T. O’Connor), Core C, “Adrenergic Receptor and Intracellular Signalling Core” – $84,286 1999-00 Univ. of Calif. University-wide AIDS Research Program, “CXCR4 Receptor Signalling and Desensitization” – $60,400 1999-00 Cystic Fibrosis Foundation, “Cyclic AMP Signaling in Airway Epithelial Cells” – $75,000 1999-00 Elsa U. Pardee Fndn., “Tumor-Cell Killing by Cyclic AMP” – $97,620 1999-04 NIH, "Signaling in Anion Trasport and Inflammation in Cystic Fibrosis" (PI, P. Quinton), Project 3, "Cyclic AMP signalling in epithelial cells" – $96,120 2000-04 NIH, "Molecular Physiology of Heart Failure" (PI, K. Chien), Project 4, “Adenylate Cyclase Regulation and Heart Failure” – $140,866 2000-04 NIH/NHLBI, "Gene Transfer of Adenylyl Cyclase to Cardiovascular Cells" – $200,000 2001-05 NIH, ”Autonomics Pharmacodynamics Pharmacogenomics – Receptors and Signal Transduction" (PI, D. O'Connor); Core D – $168,921 2001-05 NIH, Gene Therapy: From Basic Science to Clinical Practice (PI, H.K. Hammond); Project 2, "Gene Transfer of Adenylyl Cyclase to Pulmonary Smooth Muscle Cells" – $175,997 2002-06 NIH/NIGMS, "-adrenergic Receptors of S49 Lymphoma Cells" – $225,000 2003-07 NIH/NIGMS, "Hypertonic Saline and Immune Function," (PI, W. Junger) –$200,000 2003-05 SIRA, “Cell Death in Colon Cancer Cells” (Co-PI with Lars Eckman) - $120,000 2004-08 NIH/NIGMS ”Adrenergic and Purinergic Regulation of Target Cells— $217,500 2005-08 Lymphoma and Leukemia Society SCOR, Unit 3 PI: “Targeting cyclic nucleotide phosphodiesterase in the treatment of CLL”-$195,000. 2005-10 NIH “Sympathetic Neuroeffector Junctions and Blood Pressure - Core E, “Biochemistry and Signal Transduction” (Co-PI with M. Ziegler): – $50,000 Student and Trainee Supervision: UCSF (1975-78) UCSD (1978-present) Medical Student Research Fellows: Michael Shear, Lloyd Stoolman, Robert Goldfein, John Peabody Clinical Pharmacology Fellows: Michael Johnson, M.D., Michael Kennedy, M.D. Graduate Students Supervised for Ph.D. Marshall Snavely (1979-84) Lawrence Mahan (1980-84) Sandra Slivka (1983-88) Jeffrey Jasper (1985-90) Barbara Weiss (1987-90) Bonnie Firestein (1991-95) Sheryl Denker (1992-97) Alex Zambon (1997-00) Brian Head (2001-05) James Swaney (2001-05) David Arthur (2001-06) Page 6 Ross Corriden (2002-) Graduate Students on Rotation: Brian Guth, Susan Brown, Paul Shapiro, Susannah Chang, Dale Schenk, Eric First, Jan McCallister, Gary Heiserman, Teresa Donch, James Moberly, Grai Andreason, Michael Boyle, Alan Fong, Bruce Gill, Scott Struthers, Jeff Frost, Joel Zhou, Tso-Pang Yao, Sonia Villejas, Jody Martin, Karen Anderson, Elizabeth Peters, David Dirig, Fran Putkey, Dan Kalafus, Brian Molles, Melissa Betts, Alex Zambon, Amy Bower, Jason Trama, Scott Burkette, Jennifer Knox, Michael Morrisette, Helen Chen, Kevin Ring, Brett Crawford, Dan Shen, Jon Violin, Scott Coleman, Brett Holquin, David Arthur, Matt Pecot, Brian Head, James Swaney, Chris Means, Ryan Hibbs, Kathryn Nguyen, Ross Corriden, Mike Griffin, John Mongan, Christine Gould, Jacob Gump, Valerie Vitkauskas, Yousuke Horikawa, Chen-Ting Ma, Rhiannon Nolin, Sean Kandel, Cynthia Perry Medical Students Supervised for Independent Study: Daniel Sternfeld, Karin Klijn, *Carlos Corvera, Frank Ho, *J. Paul Jacobson, Allison Nguyen, Matt Bengard, Jason Etzel (Duke University) (*Graduating Medical Student Research Prize) Undergraduate Independent Research Students: Michael Honeysett, Michael Boyle, Foster Carr, Lisa Tall, Daryn Sperling, Virginia Simnad, Blair Conner, Brian Fong, Kirsten Lowrey, Susanne Pederson, Kevin Menkes, Carol Yeo, Cecilia Kao, Jonathon Holt, Derek Guist, Julie Wu, Jason Hofer, Will Ferguson, Kathryn Nguyen, Ross Corriden, Linda Pan, Jean-Paul Strong, Catherine Tzao, Hunaid Gurji, Aaron Nagiel, Kimber Bennett, Nicole Chin, Michael Hwang, Jessica Witherspoon, Madeline Lancaster, LeMar Slater, Darwin Castillo, Ryan Suda, Magnus Lu, Nicolas Boehling, Heidi Petersen, Sean Georgi, Joseph Kaminsky, Tracy Yep, Daisy Chou, Steve Fuhs Postdoctoral Fellows: Mark Dibner, Ph.D. Frederick Darfler, Ph.D. Richard Hughes, Ph.D. Harvey Motulsky, M.D. Kathryn Meier, Ph.D. Peter Munzel, Ph.D. Lawrence Mahan, Ph.D. Bruce Terman, Ph.D. Alan Maisel, M.D. Ruth McKernan, Ph.D. Marthe Howard, Ph.D. Jerene Waite, Ph.D. Martin Michel, Ph.D. Lennart Ransnäs, M.D., Ph.D. Catherine Godson, Ph.D. Denis Leiber, Ph.D. Douglas Craig, Ph.D. Cynthia Jackson, Ph.D. Kazushi Urasawa, M.D., Ph.D. Steven Post, Ph.D. Naoki Mochizuki, M.D., Ph.D. Daniel Kiel, Ph.D. Maria Balboa, Ph.D. Greg Shen, Ph.D. Mingzhao Xing, M.D., Ph.D. Ginell Post, M.D., Ph.D. Rainer Büscher, M.D. Volker Herrmann, M.D. Michael Samardzija, Ph.D. Kuniko Horie, M.D., Ph.D. Rennolds Ostrom, Ph.D. Lizhen Yan, Ph.D. Brinda K. Rana, Ph.D. Lingzhi Zhang, Ph.D. Page 7 1979-80 1979-82 1979-82 1980-83 1981-84 1983-84 1984-85 1984-87 1984-86 1985-87 1985-88 1986-90 1987-89 1986-88 1988-91 1989-91 1991-92 1990-93 1990-93 1992-95 1993-95 1993-95 1993-95 1993-97 1994-97 1995-96 1996-98 1996-99 1997-98 1997-00 1998-01 1999 1999-01 2000; 2002-04 Xiaoqiu Liu, Ph.D Richard Bundey, Ph.D. Shelli Kirstein, Ph.D. Chih-Min Tang, Ph.D. Hiroshi Nishihara, M.D., Ph.D Hemal Patel, Ph.D. Fiona Murray, Ph.D. Neil Siecke, M.D. Evan Riddle, Ph.D. Lin Wang, M.D., Ph.D. Utako Yokoyama, M.D., Ph.D. Visiting Scientists: Esam Moustafa, Ph.D. Rainer Box, Ph.D. Adolpho Garcia-Sainz, Ph.D. Petr Svoboda, Ph.D. Adawia Alousi, Ph.D. Adela Sanchez, Ph.D. Jesus Saiz, Ph.D. Jose Camacho, M.D. Lars Christian Rump, M.D. Luisa Iacovelli, M.D. Christine Bohmann, Ph.D. Stefania Mariggio Darakhshanda Shehnaz, Ph.D. Tetsuo Shiina, Ph.D. Rainer Buscher, M.D. Andre Hornung, M.D. 2000-03 2001-04 2002-05 2002-05 2002-04 2002-05 2003-present 2003-05 2003-05 2005-present 2005-present 1980-81 1987 1987 1986,1988 1989-92 1991-92 1991-92 1992-96 1995 1995 1996 1997 1998-99 2000-02 2004 2004 BIBLIOGRAPHY Original Articles 1. Wolter JR, Insel PA, Willey EN. Eye pathology following maternal rubella: A study on four children. J Ped Ophth, 3:29-35, 1966. 2. Sherwin RS, Kramer KJ, Tobin JD, Insel PA, Liljenquist JE, Berman M, Andres R. A model of the kinetics of insulin in man. J Clin Invest, 53(5):1481-1492, 1974. 3. Insel PA, Liljenquist JE, Tobin JD, Sherwin RS, Watkins P, Andres R, Berman M. Insulin control of glucose metabolism in man: A new kinetic analysis. J Clin Invest, 55(5):1057-1066, 1975. 4. Insel P, Balakir R, Sacktor B. The binding of cyclic AMP to renal brush border membranes. J Cyclic Nucleotide Res, 1:107-122, 1975. 5. Insel PA, Bourne HR, Coffino P, Tomkins GM. Cyclic AMP dependent protein kinase: Pivotal role in regulation of enzyme induction and growth. Science, 190:896-898, 1975. 6. Hochman J, Insel PA, Bourne HR, Coffino P, Tomkins GM. A structural gene mutation affecting the regulatory subunit of cyclic AMP-dependent protein kinase in mouse lymphoma cells. Proc Nat Acad Sci USA, 72(12):50515055, 1975. 7. Coffino P, Bourne HR, Hochman J, Insel P, Melmon KL, Tomkins GM. Hormone mediated lymphoma cell death: Mechanism of glucocorticoid and cyclic AMP action. J Invest Dermatol, 67(5):648-649, 1976. Page 8 8. Insel PA, Maguire ME, Gilman AG, Bourne HR, Coffino P, Melmon KL. Beta adrenergic receptors and adenylate cyclase: Products of separate genes? Mol Pharmacol, 12:1062-1069, 1976. 9. Shear M, Insel PA, Melmon KL, Coffino P. Agonist-specific refractoriness induced by isoproterenol: Studies with mutant cells. J Biol Chem, 251(23):7572-7576, 1976. 10. Hochman J, Bourne HR, Coffino P, Insel PA, Krasny L, Melmon KL. Subunit interaction in cyclic AMP-dependent protein kinase of mutant lymphoma cells. Proc Nat Acad Sci USA, 74(3):1167-1171, 1977. 11. Roberts J, Insel PA, Goldfien RD, Goldfien A. Identification of beta-adrenergic binding sites in rabbit myometrium. Endocrinology, 101:1839-1843, 1977. 12. Roberts JM, Insel PA, Goldfien RD, Goldfien A. -adrenoreceptors but not -adrenoreceptors increase in rabbit uterus with oestrogen. Nature, 270:624-625, 1977. 13. Coffino P, Bourne HR, Insel PA, Melmon KL, Johnson G, Vigne J. Studies of cyclic AMP action using mutant tissue culture cells. In Vitro, 14(1):140-145, 1978. 14. Insel PA, Fenno J. Cyclic AMP-dependent protein kinase mediates a cyclic AMP-stimulated decrease in ornithine and S-adenosylmethionine decarboxylase activities. Proc Nat Acad Sci USA, 75(2):862-865, 1978. 15. Insel PA, Stoolman LM. Radioligand binding to beta-adrenergic receptors of intact cultured S49 cells. Pharmacol, 14:549-561, 1978. 16. Insel PA, Kennedy MS. Colchicine potentiates -adrenoceptor-stimulated cyclic AMP in lymphoma cells by an action distal to the receptor. Nature, 273(5662):471-473, 1978. 17. Galant SP, Underwood S, Duriseti L, Insel PA. Characterization of high-affinity 2-adrenergic receptor binding of (–)-[3H]-dihydroalprenolol to human polymorphonuclear cell particulates. J Lab Clin Med, 92(4):613-618, 1978. 18. Insel PA, Nirenberg P, Turnbull J, Shattil SJ. Relationships between membrane cholesterol, -adrenergic receptors, and platelet function. Biochemistry, 17:5269-5274, 1978. 19. Kaywin P, McDonough M, Insel PA, Shattil SJ. Platelet function in essential thrombocythemia: Decreased epinephrine responsiveness due to a deficiency of platelet -adrenergic receptors. New Engl J Med, 299:505-509, 1978. 20. Galant SP, Duriseti L, Underwood S, Insel PA. Decreased beta-adrenergic receptors on polymorphonuclear leukocytes after adrenergic therapy. New Engl J Med, 299:933-936, 1978. 21. Johnson GL, Bourne HR, Gleason MK, Coffino P, Insel PA, Melmon KL. Isolation and characterization of S49 lymphoma cells deficient in beta-adrenergic receptors: Relation of receptor number to activation of adenylate cyclase. Mol Pharmacol, 15:16-27, 1979. 22. Roberts JM, Goldfien RD, Tsuchiya AM, Goldfien A, Insel PA. Estrogen treatment decreases -adrenergic binding sites on rabbit platelets. Endocrinology, 104(3):722-728, 1979. 23. Kennedy MS, Insel PA. Inhibitors of microtubule assembly enhance beta-adrenergic and prostaglandin E1stimulated cyclic AMP accumulation in S49 lymphoma cells. Mol Pharmacol, 16:215-223, 1979. 24. Insel PA, Sanda M. Temperature-dependent changes in binding to -adrenergic receptors of intact S49 lymphoma cells. Implications for the state of the receptor that activates adenylate cyclase under physiological conditions. J Biol Chem, 254:6554-6559, 1979. Page 9 Mol 25. Kaiser N, Bourne HR, Insel PA, Coffino P. Regulation of phosphodiesterase and ornithine decarboxylase by cAMP is cell cycle independent. J Cell Physiol, 101(3):369-374, 1979. 26. Galant SP, Duriseti L, Underwood S, Allred S, Insel PA. Beta-adrenergic receptors of polymorphonuclear particulates in bronchial asthma. J Clin Invest, 65(3): 577-585, 1980. 27. Darfler FJ, Murakami H, Insel PA. Growth of T-lymphoma cells under serum-free medium: Lack of involvement of the cyclic AMP pathway in long-term cultures. Proc Nat Acad Sci USA, 77(10):5993-5997, 1980. 28. Motulsky HJ, Shattil SJ, Insel PA. Characterization of 2-adrenergic receptors on human platelets using [3H]yohimbine. Biochem Biophys Res Commun, 97(4):1562-1570, 1980. 29. Shattil SJ, McDonough M, Turnbull J, Insel PA. Characterization of -adrenergic receptors in human platelets using [3H]clonidine. Mol Pharmacol, 19:179-183, 1981. 30. Insel PA, Darfler FJ, Kennedy MS. Cholera toxin can recouple hormone receptors that are uncoupled from adenylate cyclase. FEBS Letters, 128(1):59-62, 1981. 31. Dibner MO, Wolfe RA, Insel PA. Replacement of serum with a defined medium increases -adrenergic receptor number in cultured glioma cells. Exp Cell Research, 131:424-427, 1981. 32. Roberts JM, Insel PA, Goldfien A. Regulation of myometrial adrenoreceptors and adrenergic response by sex steroids. Mol Pharmacol, 20:52-58, 1981. 33. Dibner MD, Insel PA. Growth of C6 glioma cells in serum-containing medium decreases -adrenergic receptor number. J Cell Physiol, 109:309-315, 1981. 34. Darfler FJ, Hughes RJ, Insel PA. Characterization of serum-induced alterations in the cyclic AMP pathway in S49 lymphoma cells. J Biol Chem, 256(16):8422-8428, 1981. 35. Dibner MD, Insel PA. Serum catecholamines desensitize -adrenergic receptors of cultured C6 glioma cells. J Biol Chem, 256(14):7343-7346, 1981. 36. Insel PA, Honeysett JM. Glucocorticoid-mediated inhibition of ornithine decarboxylase activity in S49 lymphoma cells. Proc Nat Acad Sci USA, 78(9):5669-5672, 1981. 37. Honeysett JM, Insel PA. Inhibition of ornithine decarboxylase and S-adenosylmethionine decarboxylase activities of S49 lymphoma cells by agents increasing cyclic AMP, J Cyclic Nucleotide Res, 7(15):321-332, 1981. 38. Motulsky HJ, Insel PA. [3H]Dihydroergocryptine binding to alpha-adrenergic receptors of human platelets: a reassessment using the selective radioligands [3H]prazosin, [3H]yohimbine, and [3H]rauwolscine. Biochem Pharmacol, 31(16):2591-2597, 1982. 39. Darfler FJ, Insel PA. Serum-free culture of resting, PHA-stimulated and transformed lymphoid cells, including hybridomas. Exp Cell Res, 138:287-295, 1982. 40. Karliner JS, Motulsky HJ, Insel PA. Apparent "down regulation" of human platelet alpha 2-adrenergic receptors is due to retained agonist. Mol Pharmacol, 21:36-43, 1982. 41. Karliner JS, Motulsky HJ, Dunlap J, Brown JH, Insel PA. Verapamil competitively inhibits 1-adrenergic and muscarinic but not -adrenergic receptors in rat myocardium. J Cardiovas Pharmacol, 4:515-520, 1982. Page 10 42. Hughes RJ, Boyle MR, Brown D, Taylor PW, Insel PA. Characterization of coexisting alpha 1- and beta2-adrenergic receptors on a cloned smooth muscle line, BC3H-1. Mol Pharmacol, 22:258-266, 1982. 43. Snavely MD, Insel PA. Characterization of alpha-adrenergic receptor subtypes in the rat renal cortex: differential regulation of alpha1- and alpha2-receptors by guanyl nucleotides and Na+. Mol Pharmacol, 22:532-546, 1982. 44. Snavely MD, Motulsky HJ, O'Connor DT, Ziegler M, Insel PA. Adrenergic receptors in experimental and clinical pheochromocytoma. Clin Exp Hypertens, A4 (4,5):829-848, 1982. 45. Insel PA, Stengel D, Ferry N, Hanoune J. Regulation of adenylate cyclase of human platelet membranes by forskolin, J Biol Chem, 257(13):7485-7490, 1982. 46. Insel PA, Koachman AM. Cytochalasin-B enhances hormone and cholera toxin-stimulated cyclic AMP accumulation in S49 lymphoma cells. J Biol Chem, 257(16):9717-9723, 1982. 47. Motulsky HJ, Hughes RJ, Brickman A, Farfel Z, Bourne HR, Insel PA. Platelets of pseudohypoparathyroidism patients: Evidence that distinct receptor-cyclase coupling proteins mediate stimulation and inhibition of adenylate cyclase. Proc Natl Acad Sci USA, 79:4193-4197, 1982. 48. Snavely MD, Motulsky HJ, Moustafa E, Mahan LC, Insel PA. -adrenergic subtypes in the rat renal cortex: selective regulation of 1-adrenergic receptors by pheochromocytoma. Circ Res, 51(4):504-513, 1982. 49. Darfler FJ, Mahan LC, Koachman AM, Insel PA. Stimulation by forskolin of intact S49 lymphoma cells involves the nucleotide regulatory protein of adenylate cyclase. J Biol Chem, 257(20):11901-11907, 1982. 50. Stengel D, Guenet L, Desmier M, Insel P, Hanoune J. Forskolin requires more than the catalytic unit to activate adenylate cyclase. Mol and Cell Endocrinol, 28:681-690, 1982. 51. Hughes RJ, Insel PA. Human platelets are defective in processing of cholera toxin. Biochem J, 212:669-678, 1983. 52. Motulsky HJ, O'Connor DT, Insel PA. Platelet 2-adrenergic receptors in treated and untreated essential hypertension. Clin Science, 64:265-272, 1983. 53. Motulsky HJ, Snavely MD, Hughes RJ, Insel PA. Interaction of verapamil and other calcium channel blockers with 1- and 2-adrenergic receptors. Circ Res, 52(2):226-231, 1983. 54. Motulsky HJ, Insel PA. The influence of sodium on the 2-adrenergic receptor system of human platelets: Role for intraplatelet sodium in receptor binding. J Biol Chem, 258(6):3913-3919, 1983. 55. Darfler FJ, Insel PA. Clonal growth of lymphoid cells in serum-free media requires elimination of H202 toxicity. J Cell Physiol, 115:31-36, 1983. 56. Snavely MD, Mahan LC, O'Connor DT, Insel PA. Selective down-regulation of adrenergic receptor subtypes in tissues from rats with pheochromocytoma. Endocrinol, 113(1):354-361, 1983. 57. Meier KE, Snavely MD, Brown SL, Brown JH, Insel PA. 1- and 2-adrenergic receptor expression in the MadinDarby canine kidney epithelial cell line. J Cell Biol, 97:405-415, 1983. 58. Insel PA, Mahan LC, Motulsky HJ, Stoolman LM, Stoolman LM, Koachman AM. Time-dependent decreases in binding affinity of agonists for -adrenergic receptors of intact S49 lymphoma cells: A mechanism of desensitization. J Biol Chem, 258(22):13597-13605, 1983. Page 11 59. Motulsky HJ, Insel PA. ADP- and epinephrine-elicited release of [3H]guanylylimidodiphosphate from platelet membranes: implications for receptor-Ni stoichiometry. FEBS Letters, 164(1):13-16, 1983. 60. Mahan LC, Insel PA. Use of superoxide dismutase and catalase to protect catecholamines from oxidation in tissue culture studies. Analytical Biochemistry, 136:208-216, 1984. 61. Moustafa E, Snavely MD, Insel PA. Selective inhibition by organic mercurials of binding to the beta 1 population of rat renal cortical beta-adrenergic receptors. Biochem Pharmacol, 33(7):1148-1151, 1984. 62. Münzel PA, Healy DP, Insel PA. Autoradiographic localization of -adrenergic receptors in rat kidney slices using [125I]iodocyanopindolol. Am J Physiol, 246:F240-F245, 1984. 63. Meier KE, Sternfeld DR, Insel PA. Alpha1- and beta2-adrenergic receptors co-expressed on cloned MDCK cells are distinct glycoproteins. Biochem Biophys Res Commun, 118(1):73-81, 1984. 64. Darfler FJ, Mullen MD, Insel PA. Serum-stimulated cyclic-AMP production in S49 lymphoma cells grown in serumfree medium. Biochim Biophys Acta, 803:203-209, 1984. 65. Motulsky HJ, Maisel AS, Snavely MD, Insel PA. Quinidine is a competitive antagonist at 1- and 2-adrenergic receptors. Circ Res, 55(3):376-381, 1984. 66. Maisel AS, Motulsky HJ, Insel PA. Hypotension after quinidine plus verapamil: Possible additive competition at alpha-adrenergic receptors. N Engl J Med, 312:167-170, 1985. 67. Meier KE, Sperling DN, Insel PA. Agonist-mediated regulation of alpha1- and beta2-adrenergic receptor in cloned MDCK cells, Am J Physiol Cell Physiol, 246:C69-C77, 1985. 68. Mahan LM, Koachman AM, Insel PA. Genetic analysis of -adrenergic receptor internalization and down regulation, Proc Natl Acad Sci USA, 82(1):129-133, 1985. 69. Snavely MD, Ziegler MG, Insel PA. A new approach to determine rates of receptor appearance and disappearance in vivo: Application to agonist-mediated down-regulation of rat renal cortical 1- and 2-adrenergic receptors. Mol Pharmacol, 27:19-26, 1985. 70. Mahan LC, Motulsky HJ, Insel PA. Do agonists promote rapid internalization of -adrenergic receptors? Proc Natl Acad Sci USA, 82(19):6566-6570, 1985. 71. Healy DP, Münzel PA, Insel PA. Localization of 1- and 2-adrenergic receptors in rat kidney by autoradiography, Circ Res, 57:278-284, 1985. 72. Insel PA, Motulsky HJ, Mahan LC. Regulation of cyclic AMP accumulation in lymphoid cells. Proc Soc Exp Biol Med, 179:472-478, 1985. 73. Wheeler AH, Ziegler MG, Insel PA, Motulsky HJ. Episodic catatonia, hypertension, and tachycardia: elevated plasma catecholamines. Neurology, 35:1053-1055, 1985. 74. DeBlasi A, Liportiti H, Motulsky HJ, Insel PA, Fratelli W. Agonist-induced redistribution of beta-adrenergic receptors on intact human mononuclear leukocytes: redistributed receptors are nonfunctional. J Clin Endocrinol Metab, 61:1081-1088, 1985. 75. Snavely MD, Ziegler MG, Insel PA. Subtype-selective down-regulation of rat renal cortical alpha- and betaadrenergic receptors by catecholamines, Endocrinology, 117:2182-2189, 1985. Page 12 76. Insel PA, Snavely MD, Healy DP, Münzel PA, Potenza CL, Nord EP. Radioligand binding and functional assays demonstrate postsynaptic alpha2-receptors on proximal tubules of rat and rabbit kidney. J Cardiovasc Pharmacol, 7(suppl.8):S9-S17, 1985. 77. Maisel AS, Motulsky HJ, Insel PA. Externalization of -adrenergic receptors promoted by myocardial ischemia. Science, 230:183-186, 1985. 78. Hughes RJ, Insel PA. Agonist-mediated regulation of 1- and 2- adrenergic receptor metabolism in a muscle cell line, BC3H-1. Mol Pharmacol, 29:521-530, 1986. 79. Motulsky HJ, Cunningham EMS, DeBlasi A, Insel PA. Desensitization and redistribution of -adrenergic receptors on human mononuclear leukocytes. Am J Physiol, 250:E583-E590, 1986. 80. Terman BI, Insel PA. Photoaffinity labeling of 1-adrenergic receptors of rat heart. J Biol Chem, 261(12):56035609, 1986. 81. Motulsky HJ, Shattil SJ, Ferry N, Rozansky D, Insel PA. Desensitization of epinephrine-initiated platelet aggregation does not alter binding to the 2-adrenergic receptor or receptor coupling to adenylate cyclase. Mol Pharmacol, 29:1-6, 1986. 82. Mahan LC, Insel PA. Expression of -adrenergic receptors in synchronous and asynchronous S49 lymphoma cells, I. Receptor metabolism after irreversible blockade of receptors and in cells traversing the cell division cycle, Mol Pharmacol, 29:7-15, 1986. 83. Mahan LC, Insel PA. Expression of -adrenergic receptors in synchronous and asynchronous S49 lymphoma cells, I. Relationship between receptor number and response. Mol Pharmacol, 29:16-22, 1986. 84. Shattil SJ, Motulsky HJ, Insel PA, Flaherty L, Brass LF. Expression of fibrinogen receptors during activation and subsequent desensitization of human platelets by epinephrine. Blood, 68(6):1224-1231, 1986. 85. Maisel AS, Motulsky HJ, Ziegler MG, Insel PA. Ischemia- and agonist-induced changes in - and -adrenergic receptor traffic in guinea pig hearts. Am J Physiol, 253 (Heart CRC Physiol 22) H1159-H1166, 1987. 86. Maisel AS, Motulsky HJ, Insel PA. Propranolol treatment externalizes -adrenergic receptors in guinea pig myocardium and prevents further externalization by ischemia. Circ Res, 60(1):108-112, 1987. 87. Terman BI, Slivka SR, Hughes RJ, Insel PA. 1-adrenergic receptor-linked guanine nucleotide-binding protein in muscle and kidney epithelial cells. Mol Pharmacol, 31:12-20, 1987. 88. Slivka SR, Insel PA. 1-adrenergic receptor-mediated phosphoinositide hydrolysis and prostaglandin E 2 formation in Madin-Darby canine kidney cells: possible parallel activation of phospholipase C and phospholipase A 2. J Biol Chem, 262(9):4200-4207, 1987. 89. Nord EP, Howard MJ, Hafezi A, Moradeshagi P, Vaystub S, Insel PA. Alpha 2 adrenergic agonists stimulate Na+-H+ antiport activity in the rabbit renal proximal tubule. J Clin Invest, 80(6):1755-1762, 1987. 90. Howard MJ, Mullen MD, Insel PA. Amiloride interacts with renal - and -adrenergic receptors. Am J Physiol, 253:F21- F25, 1987. 91. Insel PA, Howard MJ, Motulsky HJ, Hughes RJ. Amiloride: An -adrenergic antagonist in platelets, kidney, and muscle cells. J Cardiovasc Pharmacol, 10(Supp 4):S11-S14, 1987. Page 13 92. Hughes RJ, Struthers RS, Fong AM, Insel PA. Regulation of atrial natriuretic peptide receptor on a smooth muscle cell line. Am J Physiol Cell Physiol, 253(6):C809-C816, 1987. 93. Lotersztajn S, Pavoine C, Mallat A, Stengel D, Insel PA, Pecker F. Cholera toxin blocks glucagon-mediated inhibition of the liver plasma membrane (Ca2+-Mg2+)-ATPase. J Biol Chem, 262(7):3114-3117, 1987. 94. Howard MJ, Hughes RJ, Motulsky HJ, Mullen M, Insel PA. Interaction of amiloride with - and -adrenergic receptors: Amiloride reveals an allosteric site on 2-adrenergic receptors. Mol Pharmacol, 32:53-58, 1987. 95. McKernan RM, Howard MJ, Motulsky HJ, Insel PA. Compartmentation of 2-adrenergic receptors of human erythroleukemia (HEL) cells. Mol Pharmacol, 32:258-265, 1987. 96. Henry DY, Gueritte-Voegelein F, Insel PA, Ferry N, Bouguet J, Potier P, Sevenet T, Hanoune J. Isolation and characterization of 9-hydroxy-10-trans, 12-cis-octadecadienoic acid, a novel regulator of platelet adenylate cyclase from Glechoma hederacea L. Labiatae. Eur J Biochem, 170:389-394, 1987. 97. Hughes RJ, Struthers RS, Fong AM, Insel PA. Receptor-induced degradation of atrial natriuretic peptide receptors in a smooth muscle cell. Mol Endocrinol, 2:117-124, 1988. 98. Hughes RJ, Mahan LC, Insel PA. Certain ß-blockers can decrease ß-adrenergic receptor number II. Down regulation of receptor number by alprenolol and propranolol in cultured lymphoma and muscle cells. Circ Res 63(2):279-286, 1988. 99. Maisel AS, Ziegler MG, Carter S, Insel PA, Motulsky HJ. In vivo regulation of -adrenergic receptors on mononuclear leukocytes and heart: Assessment of receptor compartmentation after agonist infusion and acute aortic constriction in guinea pigs. J Clin Invest, 82(6):2038-2044, 1988. 100. Terman BI, Reece JF, Brown RD, Insel PA. The oligosaccharide component of 1-adrenergic receptors from BC3H1 and DDT1 muscle cells: studies with glycosidases and photoaffinity labelling of intact cells. Biochem J, 253:363370, 1988. 101. Slivka SR, Insel PA. Phorbol ester and neomycin dissociate bradykinin receptor-mediated arachidonic acid release and phosphoinositide hydrolysis in Madin-Darby Canine Kidney cells. J Biol Chem, 263(29):14640-14647, 1988. 102. Jasper JR, Motulsky HJ, Insel PA. Characterization of a bromoacetylated derivative of pindolol as a high affinity, irreversible beta adrenergic antagonist in cultured cells. J Pharmacol Exp Ther, 244(3):820-824, 1988. 103. McKernan RM, Strickland WR, Insel PA. Selective blockade and recovery of cell surface 2-adrenergic receptors in human erythroleukemia (HEL) cells. Studies with the irreversible antagonist benextramine. Mol Pharmacol, 33:5157, 1988. 104. Terman BI, Insel PA. Use of 1-deoxymannojirimycin to show that complex oligosaccharides regulate cellular distribution of the alpha1-adrenergic receptor glycoprotein in BC3H-1 muscle cells. Mol Pharmacol, 38:8-14, 1988. 105. Slivka SR, Meier KE, Insel PA. 1-adrenergic receptors promote phosphatidylcholine hydrolysis in MDCK-D1-cells: A mechanism for rapid activation of protein kinase C. J Biol Chem, 263(25):12242-12246, 1988. 106. Ransnäs LA, Insel PA. Quantitation of the guanine nucleotide binding regulatory protein G s in S49 lymphoma cell membranes using antipeptide antibodies to s. J Biol Chem, 263(19):9482-9485, 1988. 107. Jasper JR, Michel MC, Insel PA. The molecular mechanism of -adrenergic receptor blockers with intrinsic sympathomimetic activity. FASEB J, 2:2891-2894, 1988. Page 14 108. Ransnäs LA, Insel PA. Subunit dissociation is the mechanism for hormonal activation of the Gs protein in native membranes. J Biol Chem, 263(33):17239-17242, 1988. 109. Hammond HK, Ransnäs LA, Insel PA. Noncoordinate regulation of cardiac G s proteins and -adrenergic receptors by a physiological stimulus, chronic dynamic exercise. J Clin Invest, 82(6):2168-2171, 1988. 110. Ransnäs LA, Insel PA. Quantitation of a guanine nucleotide binding regulatory protein by an enzyme-linked immunosorbent competition assay. Anal Biochem, 176:185-190, 1989. 111. Weiss BA, Slivka SR, Insel PA. Defining the role of protein kinase C in epinephrine- and bradykinin-stimulated arachidonic acid metabolism in Madin-Darby canine kidney cells. Mol Pharm, 36:317-326, 1989. 112. Michel MC, Brodde O-E, Schnepel B, Behrendt J, Tschada R, Motulsky HJ, Insel PA. [ 3H]idazoxan and some other 2-adrenergic drugs also bind with high affinity to a nonadrenergic site. Mol Pharmacol, 35:324-330, 1989. 113. Ransnäs LA, Svoboda P, Jasper JR, Insel PA. Stimulation of -adrenergic receptors of S49 lymphoma cells redistributes the subunit of the stimulatory G protein between cytosol and membranes. Proc Nat Acad Sci, USA, 86:7900-7903, 1989. 114. Jasper JR, Motulsky HJ, Mahan LC, Insel PA. -Adrenoceptor metabolism in wild-type, Gs, and protein kinase A-variant S49 cells. Am J Physiol Cell Physiol, 259(1):C41-C46, 1990. 115. Howard MJ, Insel PA. Elevated extracellular K+ enhances arachidonic acid release in MDCK-D1 cells. Am J Physiol Cell Physiol, 259(2):C224-C231, 1990. 116. Maisel AS, Knowlton KU, Rearden A, Carter S, Ziegler MG, Insel PA, Michel MC. Adrenergic control of circulating lymphocyte subpopulations: Effects of congestive heart failure, dynamic exercise and terbutaline treatment. J Clin Invest, 85(2):462-467, 1990. 117. Michel MC, Regan JW, Gerhardt MA, Neubig RR, Insel PA, Motulsky HJ. Nonadrenergic [ 3H]idazoxan sites are physically distinct from 2-adrenergic receptors. Mol Pharmacol, 37:65-68, 1990. 118. Maisel AS, Lee P, Michel MC, Ziegler M, Insel PA. Pertussis toxin treatment of whole blood offers a novel approach to assess G protein function in congestive heart failure. Circulation, 81:1198-1204, 1990. 119. Jasper JR, Michel MC, Insel PA. The -adrenoceptor antagonist carteolol and its metabolite 8-hydroxycarteolol have different intrinsic sympathomimetic activities. Br J Clin Pharmacol, 30:109S-111S, 1990. 120. Slivka SR, Godson C, Insel PA. ras-transformation of MDCK cells alters responses mediated by protein kinase C without altering responses mediated by bradykinin. J Cell Biochem 42:59-70, 1990. 121. Jasper JR, Michel MC, Insel PA. Amplification of -adrenergic receptor mediated cyclic AMP by antagonists with intrinsic sympathomimetic activity. Mol Pharmacol, 37:44-49, 1990. 122. Waite JJ, Insel PA. G-protein-linked polyphosphoinositide phospholipase C activity in cell membranes from clonally derived and ras-transformed Madin-Darby canine kidney cells. Biochem Cell Biol, 68:1119-1127, 1990. 123. Godson C, Weiss BA, Insel PA. Differential activation of protein kinase C is associated with arachidonate release in MDCK-cells. J Biol Chem, 265(25):8369-8372, 1990. 124. Alousi AA, Jasper JR, Insel PA, Motulsky HJ. Stoichiometry of receptor-Gs-adenylate cyclase interaction. FASEB J, 5:2300-2303, 1991. Page 15 125. Weiss BA, Insel PA. Intracellular calcium and protein kinase C interact to regulate 1-adrenergic and bradykinin receptor-stimulated phospholipase A2 activation in MDCK cells. J Biol Chem, 266(4):2126-2133, 1991. 126. Klijn K, Slivka SR, Bell K, Insel PA. Renal 1-adrenergic receptor subtypes: MDCK-D1 cells, but not rat cortical membranes, possess a single population of receptors. Mol Pharmacol, 39:407-413, 1991. 127. Ransnäs LA, Leiber D, Insel PA. Inhibition of subunit dissociation and release of G s by somatostatin in S49 lymphoma cell membrane: Dependence on Mg++ and GTP. Biochem J, 280:303-307, 1991. 128. Hughes RJ, Howard MJ, Allen JN, Insel PA. Decreased beta2-adrenergic receptor mRNA expression in receptordeficient S49 lymphoma cells. Mol Pharmacol, 40:974-979, 1991. 129. Jasper JR, Michel MC, Insel PA. Partial agonistic activity of two irreversible inhibitors of -adrenergic receptors. Biochem Pharmacol, 42:212-214, 1991. 130. Roth DA, Urasawa K, Leiber D, Insel PA, Hammond HK. A substantial proportion of cardiac G s is not associated with the plasma membrane. FEBS Letters, 296:46-50, 1992. 131. Ransnäs LA, Jasper JR, Leiber D, Insel PA. -adrenergic receptor mediated dissociation and membrane release of the Gs protein in S49 lymphoma cell membranes. Biochem J, 283:519-524, 1992. 132. Hammond HK, Roth DA, Insel PA, Ford CE, White FC, Maisel AS, Ziegler MG, Bloor CM. Myocardial -adrenergic receptor expression and signal transduction after chronic volume overload hypertrophy and circulatory congestion. Circulation, 85:269-280, 1992. 133. Jackson CA, Michel MC, Insel PA. Expression of renal adrenergic receptor subtypes in established hypertension. J Cardiovasc Pharmacol, 19:857-862, 1992. 134. Michel MC, Jäger S, Casto R, Rettig R, Graf C, Printz M, Insel PA, Phillip T, Brodde O-E. On the role of renal adrenergic receptors in spontaneously hypertensive rats. Hypertension, 19:365-370, 1992. 135. Svoboda P, Krapil P, Insel PA, Ransnäs LA. Plasma-membrane-independent pool of -subunit of the stimulatory guanine nucleotide binding regulatory protein in a low-density-membrane fraction of S49 lymphoma cells. Eur J Biochem, 208:693-698, 1992. 136. Leiber D, Jasper JR, Alousi AA, Martin J, Bernstein D, Insel PA. Alteration in G s-mediated signal transduction in S49 lymphoma cells treated with inhibitors of microtubules. J Biol Chem, 268(6):3833-3837, 1993. 137. Michel MC, Brodde O-E, Insel PA. Are cardiac G-proteins altered in rat models of hypertension? J Hypertension, 11:355-363, 1993. 138. Godson C, Bell KS, Insel PA. Inhibition of expression of protein kinase C by antisense cDNA inhibits phorbol ester-mediated arachidonate release. J Biol Chem, 268(16):11946-11950, 1993. 139. Saffitz JE, Nash JA, Green KG, Luke RA, Ransnäs LA, Insel PA. Immunoelectron microscopic identification of cytoplasmic and nuclear Gs in S49 lymphoma cells. FASEB J, 8:252-258, 1994. 140. Blue DR, Craig DA, Ransom JT, Camacho JA, Insel PA, Clarke DE. Characterization of the alpha-1 adrenoceptor subtype mediating [3H]-arachidonic acid release and calcium mobilization in Madin-Darby canine kidney cells. J Pharmacol Exp Ther, 268(3):1588-1596, 1994. 141. Büscher R, Insel PA, Michel M. Is SDZ NVI-085 an alpha-adrenoceptor-subtype selective agonist? Life Sci, 54:991007, 1994. Page 16 142. Michel MC, Insel PA. Comparison of cloned and pharmacologically defined rat tissue 1-adrenoceptor subtypes. Naunyn-Schmiedeberg's Arch of Pharmacol, 350:136-142, 1994. 143. Balboa MA, Firestein BL, Godson C, Bell KS, Insel PA. Protein kinase C mediates phospholipase D activation by nucleotides and phorbol ester in Madin-Darby canine kidney cells. J Biol Chem, 269(14):10511-10516, 1994. 144. Jasper JR, Post SR, Desai KH, Insel PA, Bernstein D. Colchicine and cytochalasin B enhance cyclic AMP accumulation via postreceptor actions. J Pharmacol Exp Ther, 274(2):937-942, 1995. 145. Ping P, Gelzer-Bell R, Roth DA, Kiel D, Insel PA, Hammond HK. Reduced -adrenergic receptor activation decreases G-protein expression and -adrenergic receptor kinase activity in porcine heart. J Clin Invest, 95(3):1271-1280, 1995. 146. Insel PA, Urasawa K, Mochizuki N. Use of antisense cDNA to assess 1-adrenergic receptor coupling to G proteins. Pharmacol Commun, 6(1-3):73-77, 1995. 147. Post SR, Hilal-Dandan R, Urasawa K, Brunton LL, Insel PA. Quantification of signalling components and amplification in the -adrenergic-receptor–adenylate cyclase pathway in isolated adult rat ventricular myocytes. Biochem J, 311:75-80, 1995. 148. Balboa M, Balsinde J, Dennis EA, Insel PA. A phospholipase D-mediated pathway for generating diacylglycerol in nuclei from Madin-Darby canine kidney cells. J Biol Chem, 270(20):11738-11740, 1995. 149. Mochizuki M, Urasawa K, Sanchez A, Insel PA. Developmental regulation of the subunit of Gq/G11 as determined by quantitative immunoblot analysis. Biochem Biophys Res Commun, 210(2):246-252, 1995. 150. Mochizuki N, Hibi M, Kanai Y, Insel PA. Interaction of the protein nucleobindin with G i2, as revealed by the yeast two-hybrid system. FEBS Letters, 373:155-158, 1995. 151. Balboa MA, Insel PA. Nuclear phospholipase D in Madin-Darby canine kidney cells. J Biol Chem, 270(50):2984329847, 1995. 152. Godson C, Masliah E. Balboa MA, Ellisman M, Insel PA. Isoform-specific redistribution of pretein kinase C in living cells. Biochim Biophys Acta, 1313:63-71, 1996. 153. Hughes RJ, Anderson KL, Kiel D, Insel PA. Cloning of GRK2 cDNA from S49 murine lymphoma cells. Am J Physiol: Cell Physiol, 270(3):C885-C891, 1996. 154. Xing M, Insel PA. Protein kinase C-dependent activation of cytosolic phospholipase A2 and mitogen-activated protein kinase by 1-adrenergic receptors in Madin-Darby canine kidney cells. J Clin Invest, 97(5):1302-1310, 1996. 155. Firestein BL, Xing M, Hughes RJ, Corvera CU, Insel PA. Heterogeneity of P2u - and P2y-purinergic receptor regulation of phospholipases in MDCK cells. Am J Physiol, 271:F610-F618, 1996. 156. Post S, Jacobson JP, Insel PA. P2 purinergic receptor agonists enhance cAMP production in Madin-Darby canine kidney epithelial cells via an autocrine/paracrine mechanism. J Biol Chem, 271(4):2029-2032, 1996. 157. Denker S, McCaffrey JM, Palade GE, Insel PA, Farquhar MG. Differential distribution of subunits and subunits of heterotrimeric G-proteins on Golgi membranes of the exocrine pancreas. J Cell Biol, 133(5):1027-1040, 1996. Page 17 158. Lortie M, Novotny WF, Peterson OW, Vallon V, Malvey K, Mendonca M, Satriano J, Insel PA, Thomson SC, Blantz RC. Agmatine, a bioactive metabolite of arginine production: degradation, and functional effects in the rat kidney. J Clin Invest, 97(2):413-420, 1996. 159. Post SR, Aguila-Buhain O, Insel PA. A key role for protein kinase A in homologous desensitization of the 2adrenergic receptor pathway in S49 lymphoma cells. J Biol Chem, 271(2):895-900, 1996. 160. Mochizuki N, Cho G, Wen B, Insel PA. Identification and cDNA cloning of a novel human mosaic protein, LGN, based on interaction with Gi2. Gene, 181:39-43, 1996 161. Balsinde J, Balboa MA, Insel PA, Dennis E. Differential regulation of phospholipase D and phospholipase A2 by protein kinase C in P388D1, macrophages. Biochem J, 321:805-9, 1997. 162. Hughes RJ, Pasillas M, Saiz J, Jasper J, Insel PA. Decreased transcript expression coincident with impaired glycosylation in the 2-adrenergic receptor gene does not result from differences in the primary sequence. Biochim Biophys Acta, 1356:281-291, 1997. 163. Xing M, Tao L, Insel PA. Role of extracellular signal-regulated kinase and PKC in cytosolic PLA2 activation by bradykinin in MDCK-D1 cells. Am J Physiol Cell Physiol, 272(2):C1380-C1387, 1997. 164. Xing M, Firestein BL, Shen GH, Insel PA. Dual role of protein kinase C in the regulation of cPLA 2-mediated arachidonic acid release by P2u receptors in MDCK-D1 cells: Involvement of MAP kinase-dependent and -independent pathways. J Clin Invest, 99(4):805-814, 1997. 165. Dao TT, Kailasom MT, Parmer RJ, Le HV, Le Verge R, Kennedy BP, Ziegler MG, Insel PA, Wright FA, O'Connor DT. Expression of altered 2-adrenergic phenotypic traits in normotensive humans at genetic risk of hereditary (essential) hypertension. J Hypertension, 16:779-792, 1998. 166. Balboa M, Insel PA. Stimulation of phospholipase D via α 1-adrenergic receptors in MDCK cells independent of PKC and -activation. Mol Pharmacol, 53:221-227, 1998. 167. Yang M, Büscher R, Taguchi K, Insel PA, Michel MC. Protein Kinase C Does Not Mediate Phenylephrine-Induced Down-regulation of Madin-Darby CAnine Kidney Cell Alpha-1B Adrenoceptors. J Pharmacol Exp Ther, 286(1):3643, 1998. 168. Gao M, Ping P, Post S, Insel PA, Tang R, Hammond HK. Increased expression of adenylylcyclase type VI proportionately increases -adrenergic receptor-stimulated cAMP in neonatal rat cardiac myocytes. Proc Natl Acad Sci, 95:1038-1043, 1998. 169. Büscher R, Herrmann V, Insel PA. PCR-based method for identifying genetic variations in human 1B- and 2adrenergic receptors. Mol Genet Metab 64(4):266-270, 1998. 170. Post SR, Rump LC, Zambon A, Hughes RJ, Buda MD, Jacobson JP, Kao CC, Insel PA. ATP activates cAMP production via multiple purinergic receptors in MDCK-D1 epithelial cells: Blockade of an autocrine/paracrine pathway to define receptor preference of an agonist. J Biol Chem 273(36):23093-23097, 1998. 171. Xing M, Post S, Ostrom RS, Samardzija M, Insel PA. Inhibition of Phospholipase A2-mediated arachidonic acid release by cyclic AMP defines a negative feedback loop for P 2Y receptor activation in Madin-Darby Canine Kidney D1 cells. J Biol Chem, 274(15):10035-10038, 1999. 172. Büscher R, Herrmann V, Ring KM, Kailasam MT, O’Connor DT, Parmer RJ, Insel PA. Variability in phenylephrine response and essential hypertension: a search for human 1B-adrenergic receptor polymorphisms. J Pharmacol Exp Ther 291(2): 793-798, 1999. Page 18 173. Shehnaz D, Torres B, Balboa M, Insel PA. Pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS), a putative P2Y1 receptor antagonist, blocks signaling at a site distal to the receptor in Madin-Darby Canine Kidney-D1 cells. J Pharmacol Exp Ther, 292(1):346-350, 2000. 174. Ostrom RS, Violin JD, Coleman S, Insel PA. Selective enhancement of -adrenergic receptor signaling by overexpression of adenylyl cyclase type 6: co-localization of receptor and adenylyl cyclase in caveolae of cardiac myocytes. Mol Pharmacol, 57:1075-1079, 2000. 175. Ostrom RS, Gregorian C, Insel PA. Cellular release of and response to ATP as key determinants of the set-point of signal transduction pathways J Biol Chem, 275(16):11735-11739, 2000. 176. Herrmann V, Büscher R, Go MM, Ring KM, Hofer JK, Kailasam MT, O’Connor DT, Parmer RJ, Insel PA. 2adrenergic receptor polymorphisms at codon 16, cardiovascular phenotypes and essential hypertension in Whites and African Americans. Am J Hypertension, 13(9):1021-1026, 2000. 177. Horie K, Insel PA. Retrovirally-mediated transfer of a G protein-coupled receptor kinase dominant-negative mutant enhances endogenous calcitonin receptor signaling in Chinese hamster ovary cells. J Biol Chem, 275(38):2943329440, 2000. 178. Yan L, Herrmann V, Hofer JK, Insel PA. -Adrenergic receptor/cAMP-mediated signaling and apoptosis of S49 lymphoma cells. Am J Physiol Cell Physiol, 279(5):C1665-C1674, 2000. 179. Zambon AC, Hughes RJ, Mezaros JG, Wu JJ, Torres B, Brunton LL, Insel PA. P2Y2 receptor of MDCK cells: cloning, expression, and cell-specific signaling. Am J Physiol: Renal Physiol, 279(6):F1045-F1052, 2000 180. Brodde O-E, Büscher R, Tellkamp R, Radke J, Dhein S, Insel PA. Blunted cardiac responses to receptor activation in subjects with Thr164Ile 2-adrenoceptors,Circulation, 103:1048-1050, 2001. 181. Büscher R, Belger H, Eilmes KJ, Tellkamp R, Radke J, Dhein S, Hoyer PF, Michel MC, Insel PA, Brodde, O -E. In-vivo studies do not support a major functional role for the Gly 389Arg 1-adrenoceptor polymorphism in humans. Pharmacogenetics, 11:199-205, 2001 182. Rana BK, Pan L, Insel PA. Use of an in silico approach to define the gene structure of eukaryotic adenylyl cyclases. Biochem Biophys Res Commun, 285:152-157, 2001. 183. Zambon AC, Brunton LL, Barrett KE, Hughes RJ, Torres B, Insel PA. Cloning, expression, signaling mechanisms, and membrane targeting of P2Y 11 receptors in Madin Darby canine kidney cells, Mol Pharmacol, 60(1):26-36, 2001. 184. Ostrom RS, Gregorian C, Drenan RM, Gabot K, Rana BK, Insel PA. Key role for constitutive cyclooxygenase-2 of MDCK cells in basal signaling and response to released ATP, Am J Physiol: Cell Physiol, 281:C524-C531, 2001. 185. Ostrom RS, Gregorian C, Drenan RM, Xiang Y, Regan JW, Insel PA. Receptor number and caveolar colocalization determine receptor coupling efficiency to adenylyl cyclase, J Biol Chem, 276(45):42063-9, 2001. 186. Zhang L and Insel PA. Bcl-2 protects lymphoma cells from apoptosis but not growth arrest promoted by cyclic AMP and dexamethasone. Am J Physiol: Cell Physiol, 281:C1641-1647, 2001. 187. Qi A-D, Zambon AC, Insel PA, Nicholas RA. Arginine-265 at the juxtaposition of TM 6 and the 3rd extracellular loop contributes to the markedly different nucleotide selectivities of human and canine P2Y 11 receptors, Mol Pharmacol, 60(6):1375-1382, 2001. 188. Zheng B, Ma Y-C, Ostrom RS, Lavoie C, Gill GN, Insel PA, Huang X-Y, Farquhar MG. RGS-PX1: A gap for Gs and a sorting nexin in vesicular trafficking, Science, 294:1939-1942, 2001. Page 19 189. Torres B, Zambon AC, Insel PA. P2Y11 receptors activate adenylyl cyclase and contribute to nucleotide-promoted cAMP formation in MDCK-D1 cells: A mechanism for nucleotide-mediated autocrine-paracrine regulation, J Biol Chem, 277(10):7761-5, 2002. 190. Büscher R, Eilmes KJ, Grasemann H, Torres B, Knauer N, Sroka K, Insel PA, Ratjen F. β2 adrenoceptor gene polymorphisms in cystic fibrosis lung disease. Pharmacogenetics. 12:347-53, 2002. 191. Ostrom RS, Liu X, Head B, Gregorian C, Seasholtz TM, Insel PA. Localization of adenylyl cyclase isoforms and G protein-coupled receptors in vascular smooth muscle cells: expression in caveolin-rich and noncaveolin domains. Mol Pharmacol, 62(4):983-992, 2002. 192. Loomis WH, Namiki S, Ostrom RS, Insel PA and Junger WG. Hypertonic stress increases T cell interleukin-2 expression through a mechanism that involves ATP release, P2 receptor, and p38 MAPK activation. J Biol Chem, 278:4590-6, 2003. 193. Bruck H, Leineweber K, Büscher R, Ulrich A, Radke J, Insel PA and Brodde O.-E. The Gly27Glu β2-adrenoceptor polymorphism slows the onset of desensitization of cardiac functional responses in vivo. Pharmacogenetics, 13:5966, 2003. 194. Lee A, Rana BK, Schiffer H, Brann M, Insel PA, Weiner D. Distribution analysis of non-synonymous polymorphisms within the G protein-coupled receptor gene family. Genomics. 81:245-8, 2003. 195. Ostrom RS, Naugle JE, Hase M, Gregorian C, Insel PA, Brunton LL, Meszaros JG. Angiotensin II enhances adenylyl cyclase signaling via calcium-calmodulin: Gq-Gs crosstalk regulates collagen production in cardiac fibroblasts. J Biol Chem. 278: 24461-8, 2003. 196. Hughes RJ, Torres B, Zambon A, Arthur D, Bohmann C, Rump LC, Insel PA. Expression of multiple P2Y receptors by MDCK-D1 cells: P2Y1 receptor cloning and signaling. Drug Dev Res, 59:1-7, 2003. 197. Nishihara H, Kizaka-Kondoh S, Insel PA, Eckmann L. Inhibition of apoptosis in normal and transformed intestinal epithelial cells by cyclic AMP through induction of inhibitor apoptosis protein–2 (IAP-2). Proc Natl Acad Sci USA, 100:8921-6, 2003. 198. Patel HH, Fryer RM, Gross ER, Bundey RA, Hsu AK, Isbell M, Eusebi LOV, Jensen RV, Gullans SR, Insel PA, Nithipatikom K, Gross GJ. 12-Lipoxygenase in opioid-induced delayed cardioprotection: gene array, mass spectrometric and pharmacological analysis. Circ Res, 92(6):676-82, 2003. 199. Bundey RA, Insel PA. Quantification of adenylyl cyclase messenger RNA by real-time polymerase chain reaction. Anal Biochem 319:318-322, 2003. 200. Brindikova TA, Bourcier N, Torres B, Pchejetski D, Gekle M, Maximov GV, Montminy V, Insel PA, Orlov SN, Isenring P. Purinergic-induced signaling in C11-MDCK cells inhibits the secretory Na-K-Cl cotransporter. Am J Physiol Cell Physiol, 285(6):C1445-53, 2003. 201. Liu X, Ostrom RS, Insel PA. Cyclic AMP-elevating agents and adenylyl cyclase overexpression promote an antifibrotic phenotype in pulmonary fibroblasts. Am J Physiol – Cell Physiol, 286:C1089-99, 2004. 202. Ostrom RS, Bundey RA, Insel PA. Nitric oxide inhibition of adenylyl cyclase type-6 activity is dependent upon lipid rafts and caveolin signaling complexes, J Biol Chem, 279:19846–53, 2004. 203. Zhang L, Insel PA. The pro-apoptotic protein Bim is a convergence point for cAMP/protein kinase A- and glucocorticoid-promoted apoptosis of lymphoid cells. J Biol Chem, 279:20858-65, 2004 Page 20 204. Nishihara H, Hwang M, Kisaka-Kondohi S, Eckmann L, Insel PA. Cyclic AMP promotes cAMP-responsive elementbinding protein-dependent induction of cellular inhibitor of apoptosis protein-2 and suppresses apoptosis of colon cancer cells through ERK1/2 and p38 MAPK. J Biol Chem, 279:26176-83, 2004. 205. Le Corre P, Parmer RJ, Kailasam MT, Kennedy BP, Skaar T, Ho H, Leverge R, Smith DW, Ziegler MG, Insel PA, Schork NJ, Flockhart D, O'Connor DT. Human sympathetic activation by α 2-adrenergic blockade with yohimbine: Bimodal, epistatic influence of cytochrome P450-mediated drug metabolism, Clinical Pharmacology and Therapeutics, 76:139-53, 2004. 206. Chen Y, Namiki S, Rictors J, Insel PA, Junger W. Defining a putative osmoreceptor in human neutrophils that involves ATP release conversion to adenosine and activation of A 2 adenosine and P2 receptors. J Leukocyte Biol, 76:245-53, 2004. 207. Egan LJ, Smith JR, Truong F, Kirstein S, Sugimoto Y, Narumiya S, Varki NM, Insel PA, Kagnoff M, Eckmann L. Thromboxane A2 and prostacyclin receptors are opposing regulators of the severity of experimental murine colitis, manuscript submitted. 208. Head BP, Patel HH, Roth DM, Lai NC, Niesman IR, Farquhar MG, Insel PA. G-protein coupled receptor signaling components localize in both sacrolemmal and intracellular caveolin-3-associated microdomains in adult cardiac myocytes, J Biol Chem, 280 (35):31036-31044, 2005. 209. King D, Etzel JP, Chopra S, Smith J, Cadman PE, Rao F, Funk S, Rana BK, Schork NJ, Insel PA, O’Connor DT. Human response to α2-adrenergic agonist stimulation studied in an isolated vascular bed in vivo: Biphasic influence of dose, age, gender, and receptor genotype, Clin Pharmacol Exptl Therap, 77:388-403, 2005. 210. Etzel JP, Rana BK, Wen G, Parmer R, Schork N, O”Connor DT, Insel PA. Genetic variation at the human α2Badrenergic receptor locus: role in blood pressure variation and yohimbine response, Hypertension, 45:1207-13, 2005. 211. Tang CM, Hoerning A, Buescher, R, O’Connor DT, Ratjen F, Grasemann H, Insel PA. Human adenosine 2B receptor: SNP discovery and evaluation of expression in patients with cystic fibrosis, Pharmacogenetics and Genomics, 15:321-327, 2005. 212. Swaney JS, Roth DM, Olson ER, Naugle JE, Meszaros JG, Insel PA. Inhibition of cardiac myofibroblast formation and collagen synthesis by activation and overexpression of adenylyl cyclase, Proc Natl Acad Sci USA, 102:427442, 2005. 213. Arthur DB, Akassoglou K, Insel PA. P2Y2 receptor activates NGF/TrkA receptor signaling to enhance neuronal differentiation, Proc Natl Acad Sci USA, 102:19138-19143, 2005. 214. Zambon AC, Zhang L, Minovitsky S, Kanter J, Prabhakar S, Salomonis N, Vranizan K, Dubchak I, Conklin B, Insel PA. Gene expression patterns define key transcriptional events in cell-cycle regulation by cAMP and protein kinase A. Proc Natl Acad Sci USA, 102:8561-8566, 2005. 215. Akimova OA, Bouricer N, Taurin S, Bundey RA, Grygorczyk K, Gekle M, Insel PA, Dulin NO, and Orlov SN. Clsecretion in ATP-treated renal epithelial C7-MDCK cells is mediated by activation of P2Y1 receptors, phospholipase A2 and protein kinase A. J. Physiol. 568:789-801, 2005. 216. Arthur DB, Akassoglou K, Insel PA. P2Y2 receptor activates NGF/TrkA receptor signaling to enhance neuronal differentiation, Proc. Natl Acad. Sci. USA, 102:19138-19143, 2005. 217. Patel HH, Head BP, Petersen HN, Niesman IR, Feramisco JR, Farquhar MG, Gross GJ, Insel PA*, Roth DM*. Protection of adult rat cardiomyocytes from ischemic cell death: role of caveolar microdomains and opioid receptors, Am J Physiol - Heart and Cardiovascular Physiol, 291(1):H344-50, 2006 (*co-senior authors). Page 21 218. Buescher R, Hoerning A, Patel HH, Zhang S, Arthur, DB, Grasemann H, Ratjen F, Insel PA. P2Y 2 receptor polymorphisms and haplotypes in cystic fibrosis and their impact on Ca 2+ influx, Pharmacogenetics and Genomics 16:199-205, 2006. 219. Hoekstra HI, Hirschmann RJ, Bundey RA, Insel PA, Crossland JP. A single amino acid mutation contributes to adaptive beach mouse color pattern, Science 7;313(5783):101-4, 2006. 220. Riddle EL, Rana BK, Murthy KK, Rao F, Eskin E, O’Connor DT Insel PA. Polymorphisms and Haplotypes of the Regulator of G Protein Signaling-2 Gene in Normotensives and Hypertensives, Hypertension 47:3-415-420, 2006. 221. Arthur DB, Georgi S, Akassoglou K, Insel PA. Inhibition of apoptosis by P2Y2 receptor activation: novel pathways for neuronal survival, J Neuroscience, 26(14):3798 –3804, 2006. 222. Swaney JS, Patel HH, Yokoyama, U, Head BP, Roth DM, Insel PA. Focal adhesions in (myo)fibroblasts scaffold adenylyl cyclase with phosphorylated caveolin, J Biol Chem, 281:17173-79, 2006. 223. Arthur DB, Akassoglou K, Insel PA. P2Y2 and TrkA receptors interact with Src family kinase for neuronal differentiation, Biochem Biophys Res Commun, 347(3):678-82, 2006. 224. Head BP, Patel HH, Roth DM, Murray F, Swaney JS, Niesman IR, Farquhar MG, Insel PA. Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components, J Biol Chem. 2006 Jul 3; [Epub ahead of print]. 225. Bundey RA, Insel PA. Adenylyl cyclase 6 over-expression decreases the permeability of endothelial monolayers via preferential enhancement of prostacyclin receptor function, Mol Pharmacol. 2006 Aug 2 [Epub ahead of print]. 226. Arthur DB, Taupenot L, Insel PA. Nerve growth factor-stimulated neuronal differentiation induces changes in P2 receptor expression and nucleotide-stimulated catecholamine release, J Neurochem (in revision). 227. Rana BK, Insel PA, Payne S, Abel K, Beutler E, Ziegler MG, Schork NJ, O’Connor DT. A large population-based sample reveals the contribution of gene-gender interactions in blood pressure in Caucasian-Americans, Hypertension (in revision). 228. Murray F, Patel HH, Suda RYS, Zhang S, Thistlethwaite PA, Yuan JX, and Insel PA. Expression and Activity of cAMP Phosphodiesterase Isoforms in Pulmonary Artery Smooth Muscle Cells from Patients with Pulmonary Hypertension; Role of PDE1, Am J Physiology- Lung and Resp. Physiology (in revision). 229. Akimova, OA, Grygorzcyk A, Bundey RA, Gekle M, Insel PA, Orlov SG. Transient activation and delayed inhibition of NA, K+, Cl- cotransport in ATP-treated C11-MDCK cells: evidence of Ca2+-mediated and independent signalling triggered by distinct P2Y receptors, J Biol Chem (in revision). 230. Patel HH, Zhang S, Murray F, Suda R, Head BP, Yokoyama U, Swaney JS, Niesman IR, Schermuly RT, Pullamsetti S, Thistlethwaite PA, Miyanohara A, Farquhar MG, Yuan JX and Insel PA. Increased caveolin-1 and caveolae expression contribute to the pathophysiology of idiopathic pulmonary arterial hypertension (submitted). 231. Steagall WK, Barrow BJ, Glasgow CG, Woo J, Ethrmantraut M, Lin J, Insel PA, Moss J. β 2-adrenergic Receptor Polymorphisms in Cystic Fibrosis, Pharmacogenet Genomics (in revision). 232. Rana BK, Shiina T, Rao F, Mauboubi V, Kirstein SL, Ziegler MG, O’Connor DT, Insel PA. A polymorphism in the 5’ untranslated region of the dopamine D1 receptor influences receptor expression (in revision). 233. Chen Y, Corriden R, Inoue Y, Yip L, Hashiguchi, N, Zinkernagel, A, Nizet V, Insel, PA*, Junger WG*. Polarized ATP release, P2Y2 receptors, and A3 adenosine receptors guide neutrophil chemotaxis, Science (in revision). (*cosenior authors) 234. Yip L, Cheung, CW, Chen Y, Hashiguchi N, Corriden R, Insel PA, Junger WG. Expression and activity of adenosine P1 and nucleotide P2 receptors during T-cell activation (submitted). 235. Zhang S, Patel HH, Schach C, Murray F, Remillard CV, Thistlethwaite PA, Insel PA, Yuan JX-J. Pulmonary artery smooth muscle cells from normal subjects and patients with idiopathic pulmonary arterial hypertension show divergent cAMP-mediated effects on TRPC expression and capacitative Ca 2+ entry (submitted). Page 22 236. Yip L, Cheung CW, Corriden R, Chen Y, Insel PA, Junger WG. Hypertonic stress regulates T cell function by the opposing actions of extracellular ATP and adenosine (submitted). Book Chapters, Invited Publications and Review Articles 1. Insel PA, Kramer KJ, Berman M, Sherwin RS, Liljenquist JE, Tobin JD, Andres R. Modelling the insulin glucose system in man. Fed Proc 33:1865-1868, 1974. 2. Bourne HR, Coffino P, Hochman J, Insel PA, Jones P, Melmon KL, Tomkins GM. Genetic and functional studies of the cyclic AMP receptor in a cultured mammalian cell. In Proceedings of the Sixth International Congress of Pharmacology, vol. 1, Receptors and Cellular Pharmacology (E. Klinge, ed.), Pergamon Press, New York, pp 223232, 1976. 3. Coffino P, Bourne HR, Friedrich U, Insel PA, Lemaire I, Hochman J, Melmon KL, Tomkins GM. Somatic genetic analysis of cyclic AMP action. Recent Prog in Hormone Res, 32:669-682, 1976. 4. Coffino P, Gray JW, Lemaire I, Insel PA. Growth regulation by cyclic AMP in mouse lymphoma cells. In Cyclic Nucleotides and the Regulation of Cell Growth (Morad Abou-Sabe, ed.), Dowden, Hutchison and Ross, Inc., Stroudsbourg, Pennsylvania, pp 276-278, 1977. 5. Melmon KL, Insel PA. Inflamatory and immune responses: Cell individuality amidst ubiquitous hormonal signals, Johns Hopkins Med J, 141:15-22, 1977. 6. Melmon KL, Weinstein Y, Poon T, Bourne HR, Shearer GM, Coffino P, Insel PA. Receptors for low molecular weight hormones in lymphocytes. In Immunopharmacology (J.W. Hadden, R.C. Coffey and F. Spreafica, eds.), Plenum Press, New York, pp. 331-356, 1977. 7. Insel PA. Membrane-active hormones: Receptors and receptor regulation. In International Review of Biochemistry, series II, Biochemistry and Mode of Action of Hormones (H.V. Rickenberg, ed.) University Park Press, pp 1-43, 1978. 8. Miller R, Insel PA, Melmon KL. Drugs in inflammatory disease. In Clinical Pharmacology (K.L. Melmon and H.F. Morrelli, eds.), Macmillan, pp 657-708, 1978. 9. Insel PA, Shear M, Sanda M, Melmon KL, Peabody J, Kennedy M. Refractoriness to beta-adrenergic agonists in S49 cells. Frontiers in Catecholamine Research, pp. 483-485, 1979. 10. Melmon KL, Verlander M, Krasny L, Goodman M, Kaplan N, Castagaoli M, Insel PA. Chemistry and Pharmacology of Catecholamines and other low molecular weight ligands conjugated to insoluble carriers. Frontiers in Catecholamine Research, pp. 474-476, 1979. 11. Roberts JM, Goldfien RD, Goldfien A, Insel PA. Regulation of alpha-adrenergic receptors and response by estrogen. Frontiers in Catecholamine Research, pp. 441-443, 1979. 12. Roberts JM, Goldfien RD, Insel PA. Dihydroergocryptine binding and alpha-adrenoreceptors in smooth muscle. Nature (Matters Arising) 283:108-109, 1980. 13. Insel PA, Snavely MD. Catecholamines and the Kidney: Receptors and renal function. Annu Rev of Physiology, 43:625-636, 1981. Page 23 14. Insel PA. Use of a genetic approach to study cyclic AMP metabolism, in Cell Regulation by Intracellular Signals (S. Swillens and J. Dumont, eds.) NATO an Advanced Study Institutes Series, Series A: Life Sciences, Vol. 44, pp. 137-145, 1982. 15. Insel PA, Darfler FJ, Hughes RJ, Meier KE, Dibner MD, Mahan LC, Snavely MD, Boyle MR, Koachman AM. Cell biological studies of adrenergic receptors. Hormones and Cell Regulation, (Dumont JE, Nunez J, Schultz G, eds.) Elsevier Biomedical Press, Vol. 6, 167-178, 1982. 16. Darfler FJ, Insel PA. Growth of hybridomas & other lymphoid cells in hormonally defined media. Cold Spring Harbor Conference on Cell Proliferation, Growth of Cells in Defined Media, Vol. 9, pp. 717-726, 1982. 17. Motulsky HJ, Insel PA. Adrenergic receptors in man: direct identification, physiological regulation, and clinical alterations. N Engl J Med, 307:18-29, 1982. 18. Insel PA, Motulsky HJ. Calcium channel blocking agents. N Engl J Med (Letter to Editor), 308:1361-2, 1983. 19. Ferry N, Adnot S, Insel PA, Hanoune J. Le systeme adenylate cyclasique des plaquettes humaines. Nouvelle Revue Francaise d'Hematologie 25:343-353, 1983. 20. Motulsky HJ, Insel PA. The study of cell surface receptors with radioligand binding: Methodology, data analysis and experimental problems. In Receptor Science in Cardiology (S.I. Haft and J.C. Karliner, eds.). pp. 1-42, 1984. 21. Darfler FJ, Insel PA. Growth of lymphoid cells in serum-free medium. In Methods for Serum-Free Culture of Neuronal and Lymphoid Cells, Alan R. Liss, Inc.: New York, pp. 187-196, 1984. 22. Motulsky HJ, Insel PA. Radioligand binding to adrenergic receptors in humans, in Norepinephrine: Clinical Aspects (M. Ziegler and R. Lake, eds.) pp. 271-306; Wiliams and Wilkins, 1984. 23. Insel PA. Identification and regulation of adrenergic receptors in target cells. Am J Physiol Endocrinol Metab, 247-E53-E58, 1984. 24. Insel PA, Motulsky HJ. A hypothesis linking intracellular sodium, membrane receptors, and hypertension. Life Sciences 34:1009-1113, 1984. 25. Meier KE, Insel PA. Hormone receptors and response in cultured renal epithelial cell lines. In Tissue Culture of Epithelial Cells (M. Taub, ed.), Plenum Pub. Corp., pp 145-178, 1985. 26. Insel PA. Adrenergic receptors on human blood cells, in Pharmacology of Adrenoceptors (E. Szabaodi, C.M. Bradshaw, S. Nahorski, eds.), Macmillan Press, pp. 215-225, 1985. 27. Insel PA, Hughes RJ, Meier KE, Mahan LC, Snavely MD. Cellular metabolism of alpha- and beta-adrenergic receptors. Adrenergic Receptors: Molecular Properties and Therapeutic Implications (R.D. Lefkowitz, E. Lindenbaur, eds.), F. K. Schattaner Verlag., pp. 537-548, 1985. 28. Motulsky HJ, Mahan LC, Insel PA. Radioligands, agonists and membrane receptors on intact cells: data analysis in a bind. Trends in Pharmacol Sci, 6(8):317-319, 1985. 29. Insel PA. Cardiovascular alpha2 receptors (Letter to the Editor), J Mol Cell Cardiol, 18, 457-459, 1986. 30. Insel PA, Motulsky HJ. Physiologic and pharmacologic regulation of adrenergic receptors, in Adrenergic Receptors in Man (P.A. Insel, ed.) Marcel Dekker, pp. 201-236, 1987. Page 24 31. Motulsky HJ, Insel PA. In Vitro methods for studying human adrenergic receptors: Methods and applications. In Adrenergic Receptors in Man (P.A. Insel, ed.), Marcel Dekker, pp. 139-160, 1987. 32. Maisel AS, Motulsky HJ, Insel PA. Life cycles of cardiac 1 -and -adrenergic receptors. Biochem Pharmacol, 36(1):1-6, 1987. 33. Mahan LC, McKernan RM, Insel PA. Metabolism of alpha- and beta-adrenergic receptors. Annu Rev Pharmacol Toxicol, 27:215-235, 1987. 34. Insel PA, Mahan LC, Hughes RJ, Motulsky HJ, Snavely MD. Agonist-regulated metabolism of alpha- and betaadrenergic receptors. Epinephrine in the Central Nervous System, (J. Stolk, D.C.U. Prichard and K. Fuxe, eds.) pp. 188-195, 1988. 35. Insel PA, Motulsky HJ. Regulation of alpha-2 adrenergic receptors. In The Alpha2-Adrenergic Receptors (L.E. Limbird, ed.), Humana Press, pp. 281-321, 1988. 36. Insel PA, Terman BI, Slivka SR, Hughes RJ, Howard MJ. Molecular mechanisms and regulation of alpha 1adrenergic receptors. Proc. Xth Intl. Congress of Pharmacology, (M.J. Rand and C. Raper, eds.) Excerpta Media, pp.13-16, 1988. 37. Loterstajn S, Pavoine C, Mallat A, Stengel D, Insel P, Pecker F. Hormonal inhibition of the liver plasma membrane (Ca++-Mg++) ATPase is mediated by a Gs-like protein. Cell Calcium Metabolism 87, (G. Fiskum, ed.) pp. 3-11, 1988. 38. Insel PA. Phenylalkylamines are promiscuous receptor blockers. Trends in Pharmacol Sci, (Letter 9:10), 1988. 39. Insel PA. How should we study brain adrenergic receptors in aging? Neurobiol of Aging, 9:64-66, 1988. 40. Insel PA, Ransnäs LA. G proteins & cardiovascular disease. Circulation, 78:1511-1513, 1988. 41. Michel MC, Insel PA, Brodde O-E. Renal -adrenergic receptor alterations: a cause of essential hypertension? FASEB J, 3:139-144, 1989. 42. Insel PA. Adrenergic Receptors: Evolving concepts on structures and function. Am J Hypertension, 2:112S-118S, 1989. 43. Michel MC, Insel PA. Are there multiple imidazoline binding sites? Trends Pharmacol Sci, 10(9):342-344, 1989. 44. Insel PA. Structure & function of alpha-adrenergic receptors. Am J Med, 87:2A-12S-18S, 1989. 45. Insel PA, Maisel AS Alpha1- and beta-adrenergic receptors in myocardial ischemia and injury. Adrenergic System and Ventricular Arrhythmias in Myocardial Infarction (J. Brochman and A. Schomig, eds.) Springer-Verlag, pp. 8190, 1989. 46. Insel PA. Analgesic-antipyretics and anti-inflammatory agents; drugs employed in the treatment of rheumatoid arthritis and gout. In: Goodman and Gilman's Pharmacological Basis of Therapeutics, 8th edition, pp. 638-681, 1990. 47. Michel MC, Brodde O-E, Insel PA. Peripheral adrenergic receptors in hypertension. Hypertension, 16:107-120, 1990. 48. Taylor P, Insel PA Molecular basis of pharmacologic selectivity. In: Principles of Drug Action, 3rd ed., (W.B. Pratt and P. Taylor, eds.), pp. 1-102, 1990. Page 25 49. Taylor P, Insel PA. Molecular basis of drug action. In: Principles of Drug Action, 3rd ed., (W.B. Pratt and P. Taylor, eds.) pp. 103-200, 1990. 50. Insel PA, Characterization and function of renal -adrenergic receptors. Excerpta Medica, Sixth Alpha-Adrenoceptor Symposium. Tokyo, pp. 43-52, 1990. 51. Maisel AS, Ransnäs LA, Insel PA. -adrenergic receptors and the Gs protein in myocardial ischemia and injury. Basic Research in Cardiology, 85 (Suppl.):47-56, 1990. 52. Insel PA, Wasserman SI. Asthma: A disease of altered adrenergic receptor sensitivity? FASEB J, 4:2732-2736, 1990. 53. Insel PA. Characterization of 1-adrenergic receptors: structure and function. J Vasc Med Biol, 2:225-230, 1990. 54. Insel PA. G Proteins in myocardial failure. Congestive Heart Failure Index and Reviews, 3:3, 1990. 55. Insel PA. GTP-binding protein-coupled receptors. Introduction to Endocrine Invest.: Techniques and Concepts. pp. 85-92, 1990 and 1991. 56. Hughes RJ, Howard MJ, Insel PA. Beta2-adrenergic receptor regulation in receptor-deficient S49 lymphoma cells. Adrenoceptors: Structure, Mechanisms, Function (E. Szabadi and C.M. Bradshaw, eds). Birkhaüser Verlag-Basel, pp. 347-348, 1991. 57. Insel PA. ß-adrenergic receptors in pathophysiologic states and in clinical medicine. In: The Beta-Adrenergic Receptors (J.P. Perkins, ed.), pp. 295-343, 1991. 58. Insel PA, Hughes RJ, Ransnäs LA, Leiber D, Jasper JR, Motulsky HM, Alousi A. Adrenergic receptors coupled to adenylyl cyclase. Adrenoceptors: Structure, Mechanisms, Function (E. Szabadi and C.M. Bradshaw, eds). Birkhaüser Verlag-Basel, pp. 117-126, 1991. 59. Urasawa K, Insel PA. GTP-binding proteins in cardiovascular disease. (Book Chapter) G Proteins: Signal Transduction and Disease, G. Milligan and M.O. Wakelam, eds. Academic Press, pp.47-85, 1992 60. Insel PA, Weiss BA, Slivka SR, Howard MJ, Waite JJ, Godson CA. Regulation of phospholipase A 2 by receptors in MDCK-D1 cells. Biochem Soc Trans, 19:329-333, 1991. 61. Jasper JR, Insel PA. Evolving concepts of partial agonism: the -adrenergic receptor as a paradigm. Biochem Pharmacol, 43(2):119-130, 1992. 62. Insel PA. GTP-binding proteins and post-receptor components in hypertension. Pharmacol & Toxicol, 70:528-531, 1992. 63. Insel PA, Urasawa K, Leiber D, Roth D, Hammond HK. Regulation of the G s protein in the heart. New Aspects Of The Treatment Of Failing Heart. Yasuda H. and Kawaguchi, eds. Springer-Verlag: Tokyo, pp. 58-61, 1992. 64. Insel PA. Adrenergic receptors, G-proteins, and cell regulation: Implications for aging research. Experimental Gerontology, 28:341-348, 1993. 65. Insel PA, Hammond HK. -adrenergic receptors in heart failure. J Clin Invest, 92(6): 2564-5, 1993. 66. Jackson CA, Insel PA. Renal -adrenergic receptors and genetic hypertension. Ped Nephrol, 7:853-858, 1993. Page 26 67. Insel PA. GTP-binding protein-coupled receptors. Introduction to Molecular & Cellular Res, Endocrine Soc., pp. 113-120, 1993 and 1994. 68. Cohen JS, Insel PA. Weaning patients addicted to codeine for pain relief. Consultant, 36:196-97, 1996. 69. Insel PA. Adrenergic receptors: evolving concepts and clinical implications. New Engl J Med, 334:580-585, 1996. 70. Cohen JS, Insel PA. The Physician's Desk Reference: Problems and possible improvements. Arch Intern Med, 156:1375-1380,1996. 71. Insel PA. Analgesic-antipyretic and anti-inflammatory agents and drugs employed in the treatment of gout in Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th ed., pp. 617-658, 1996. 72. Michel M, Insel PA. Making sense out of antisense for the study of drug receptors. J Clin Invest, 98(5):1074-1075, 1996. 73. Insel PA, Balboa MA, Mochizuki N, Post SR, Urasawa K, Xing M. Mechanisms for activation of multiple effectors by alpha1-adrenergic receptors. Adv Pharmacol, 42: 451-453, 1997. 74. Hammond HK, Ping P, Insel PA. Cardiac G-protein receptor kinase activity: effect of a beta-adrenergic receptor antagonist. Adv Pharmacol, 42: 507-510, 1997. 75. Insel PA, Firestein BL, Xing M, Post SR, Jacobson JP, Balboa, MA, Hughes RJ. P 2 purinoceptors utilize multiple signalling pathways in MDCK D1 cells. J Autonom Pharmacol 16:311-3, 1997. 76. Varki AP, Kagnoff MF, Insel PA. Passing the baton at high speed: time to hand over to a new editorial board. J Clin Invest, 99(4):553-4, 1997. 77. Insel PA. Solo dances within a monograph. a2-Adrenergic Receptors: Structure, Function and Therapeutic Implications spotlight on alpha2-adrenergic receptors. Trends in Pharmacol Sci 19(3):116, 1997. 78. Insel PA. Agents for the treatment of gout, Quick Look: Pharmacology, Robert B. Raff and Gerald H. Sterling, eds. (book chapter). Fencecreek Publishing, 1997. 79. Insel PA, Büscher R, Xing M, Balboa M, Guist D. Mechanisms of 1B-adrenergic receptor signalling. Pharmacol & Toxicol, 83 (Suppl): 17-19, 1998. 80. Büscher R, Herrmann V, Insel PA. Human adrenoceptor polymorphisms: Evolving recognition of clinical importance. Trends in Pharmacol Sci, 20(3):94-99, 1999. 81. Balsinde J, Balboa MA, Insel PA, Dennis EA. Regulation and inhibition of phospolipase A 2. Annu Rev Pharmacol Toxicol, 39:175-189, 1999. 82. Post SR, Hammond HK, Insel PA. -adrenergic receptors and receptor signaling in heart failure. Annu Rev Pharmacol Toxicol, 39:343-60, 1999. 83. Ostrom RS, Insel PA. Caveolar microdomains of the sarcolemma: Compartmentation of signaling molecules comes of age. Circ Res, 84:1110-1112, 1999. 84. Post SR, Ostrom RS, Insel PA. Biochemical methods for detection and measurement of cyclic AMP and adenylyl cyclase activity, Adrenergic Receptor Protocols, Curtis A. Machida, ed. (book chapter), Humana Press, Inc., pp. 363-374, 2000. Also appeared in Methods in Molecular Biology, 126:363-74, 2000. Page 27 85. O’Connor DT, Insel PA, Ziegler MG, Hook VY, Smith DW, Hamilton BA, Taylor PW, Parmer RJ. Heredity and the Autonomic Nervous System in Human Hypertension. Current Hypertension Reports, 2:16-22, 2000. 86. Ostrom RS, Insel PA. Signal transduction pathways in caveolae. Science & Medicine, 7:44-53, 2000. 87. Ostrom RS, Post SR and Insel PA. Stoichiometry and compartmentation in G protein-coupled receptor signaling: implications for therapeutic interventions involving Gs. J Pharmacol Exp Ther, 294(2):407-412, 2000. 88. Glew, RH, Insel, PA. Academic health center administrators don't perish when they don't publish, Acad Med, 75(10):996-998, 2000. 89. Dempsey EC, Newton AC, Mochly-Rosen D, Fields AP, Reyland ME, Insel PA, Messing RO. EB Symposium Report: Protein kinase C isozymes and the regulation of diverse cell responses. Amer J Physiol: Lung Cell Mol Physiol, 279(3):L429-L438, 2000. 90. Rana BK, Shiina T and Insel PA. Genetic variations and polymorphisms of G-protein coupled receptor polymorphisms: functional and therapeutic implications. Annu Rev Pharmacol Toxicol, 42:593-624, 2001. 91. Insel PA, Ostrom RS, Zambon AC, Hughes RJ, Balboa MA, Shehnaz D, Gregorian C, Torres B, Firestein BL, Xing M, Post SR. P2Y receptors of MDCK cells: epithelial cell regulation by extracellular nucleotides. Clin Exp Pharmacol Physiol, 28:351-354, 2001. 92. Rana BK and Insel PA. Useful GPCR Websites. Trends Pharmacol Sci, 22(9):485-486, 2001. 93. Insel PA, Ostrom RS, Michel MC, and Büscher R. α1-Adrenergic receptors of MDCK-D1 cells utilize multiple signalling components. Catecholamine Research: Molecular Insights to Clinical Medicine, (T. Nagatsu, T. Nabeshima, R. McCarthy, D.S. Goldstein, eds.) Kluwer Academic/Plenum: New York, pp 257-60, 2002. 94. Rana BK and Insel PA. G-protein-coupled receptor websites. Trends Pharmacol Sci, 23(11):535-536, 2002. 95. Michel MC, Jasper J, Insel PA. Adrenoceptors: a convergence of thinking. Molecular Interventions, 2:198-200, 2002. 96. Ostrom RS, Rana BK and Insel PA. Stoichiometry of G Protein-coupled Receptor Signaling: Implications in the Genomic Era Pharmaceutical News. 9:295-303, 2003. 97. Insel PA and Ostrom RS. Forskolin as a tool for examining adenylyl cyclase expression, regulation and G protein signaling. Cellular and Molecular Neurobiology. 23(3):305-314, 2003. 98. Michel MC, Insel PA. Receptor gene polymorphisms: lessons on function relevance from the β 1-adrenoceptor. Brit J Pharmacol, 138:279-282, 2003. 99. Insel PA. Location, location, location. Trends Endocrinol Metab. 14(3):100-102, 2003. 100. Insel PA, Kirstein SL. Naturally occurring variants in catecholamine receptor genes. Auton Autacoid Pharmacol, 23:228-30, 2003. 101. Insel PA, Feldman R. Norepinephrine Receptors. In: Encyclopedia of Endocrine Diseases. Elsevier, 3:375-82, 2004. 102. Koopmans RP, Insel PA, Michel MC. Pharmacogenetics of hypertension treatment: a structured review, Pharmacogenetics, 13:705-13, 2003. Page 28 103. Kirstein SL, Insel PA. Autonomic nervous system pharmacogenomics: a progress report, Pharmacological Reviews, 56:31-52, 2004. 104. Tang C-M, Insel PA. GPCR expression in the heart: "New" receptors in myocytes and fibroblasts. Trends Cardiovasc Med, 14:94-9, 2004. 105. Bundey RA, Insel PA. Discrete Intracellular Signaling Domains Of Soluble Adenylyl Cyclase: Camps of cAMP? Science STKE 231:pe19, 2004. 106. Rana BK, Insel PA. Receptor databases and computational websites for ligand binding. Receptor Binding Techniques, (A Davenport, ed.), Humana Press, Totowa, NJ, 1-15, 2005. 107. Ostrom RS, Insel PA. The evolving role of lipid rafts in G protein-coupled receptor signaling: Implications for molecular pharmacology. Brit J Pharmacol, 143(2):235-45, 2004. 108. Siecke N, Insel PA. Clarifying the effects of adrenergic receptor polymorphisms by measuring synaptic parameters. J Am. College Cardiology, 44(10):2016-18, 2004. 109. Insel PA, Kornfeld S, Majerus PW, Marks AR, Marks PA, Relman AS, Scharschmidt BF, Stossel TP, Varki AP, Weiss SJ, Wilson JD. Historical highlights 1924-2004: Blasts from the past. J. Clin Invest. 114(8):1017-1033, 2004. 110. Riddle E, Schwartzman R , Bond M, Insel PA. Multi-tasking RGS Proteins in the Heart: the Next Therapeutic Target? Circulation Research, 96:401-11, 2005. 111. Michel MC, Insel PA. Adrenergic Receptors in Clinical Medicine. In: The Adrenergic Receptors in the 21st Century (D. Perez, ed.). Humana Press, 1-58829-423-4, 2005. 112. Insel PA, Head BP, Ostrom RS, Patel HH, Swaney JS, Tang C-M, and Roth DM. Caveolae and Lipid Rafts: G Protein–Coupled Receptor Signaling Microdomains in Cardiac Myocytes. Ann. NY Acad Sci., 1047:166-172, 2005. 113. Insel PA, Head BP, Patel HH, Roth DM, Bundey RA, Swaney JS. Compartmentation of G-protein-coupled receptors and their signaling components in lipid rafts and caveolae Biochem Soc Transactions, 33:1131-34, 2005. 114. Tang CM, Insel PA. Genetic variation in G-protein-coupled receptors – consequences for G-protein-coupled receptors as drug targets. Expert Opinion on Therapeutic Targets, 9:1247-65, 2005. 115. Murray F, Insel PA, Yuan JX-J. Role of O2-sensitive K+ and Ca2+ channels in the regulation of the pulmonary circulation: Potential role of caveolae and implications for high altitude pulmonary edema. Respir Physiol Neurobiol, 151:192–208, 2006. 116. Ostrom RS, Insel PA. Methods for the study of signaling molecules in lipid rafts and caveolae. In: Transmembrane Signaling Protocols. (H. Ali, B. Haribabu, ed.). Humana Press, 1-58829-546-X, 2006. 117. Murray F, Insel PA, Yuan JX. Role of O(2)-sensitive K(+) and Ca(2+) channels in the regulation of the pulmonary circulation: potential role of caveolae and implications for high altitude pulmonary edema, Respir Physiol Neurobiol, 151(2-3):192-208, 2006. 118. Insel PA, The IUPS Congress and Experimental Biology: An experiment that worked! The Physiologist (in press). 119. Head BP and Insel PA. Do caveolins regulate cells by actions outside of caveolae? Trends Cell Biol (in revision). 120. Insel PA, Tang C-M, Hahntow I, Michel M. Impact of GPCRs in clinical medicine: genetic variants and drug targets, Biochim Biophys Acta (submitted). Page 29