116102638
Phenylmethylsulphonyl
fluoride (PMSF)
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Prof. C M Thomas
Assessor
Dr L. Bingle
Assessment Number*
1818
Date of Assessment
10 / 07 / 2001
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
S101
Dr L. Bingle, Dr J. Hothersall
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Inhibition of protease activity using PMSF
AMOUNT
max/stored
Attach copies of data sheet(s)
HAZARD
RISK PHRASES
HAZDAT NO***
Phenylmethylsulphonyl
fluoride (PMSF)
5g
Toxic
Toxic by inhalation, in
contact with skin and if
swallowed.
Causes burns.
1275
Ethanol
2.5 litres
Highly
flammable
Highly flammable.
CHIPS regulations approved
supply list classification.
84
100 mM PMSF (solution in
ethanol).
2 ml
Harmful and
highly
flammable
Harmful by inhalation, in
contact with skin and if
swallowed. Highly flammable.
5
BIOSCIENCESNO***
INTENDED USE**
Give brief details and attach protocol/instructions
PMSF added to buffer used for dissolving / diluting protein samples as a
protease inhibitor. Working concentration typically 1 mM. Stock solution of PMSF (100 mM in ethanol) stored in 0.1 ml
aliquots.
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
PMSF:
Contact with skin or eyes, or inhalation of powder, could occur during preparation of stock solution.
Ethanol:
Accidental spillage of ethanol from stock bottle could result in a fire risk.
100 mM PMSF solution:
Contact with exposed skin could occur during use of stock solution.
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7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Risks and should be minimal if good chemical practice is followed. Steps described below must be taken to remove risk of
exposure by inhalation of solid PMSF.
Only small amounts of stock solution (typically <0.1 ml on any occasion) are used
8
CONTROL MEASURES
Additional to Good Chemical Practice
Eye protection, gloves and face mask should be worn when preparing stock solution. Avoid raising or inhaling
dust, eg. by dispensing solid to tared container in fume hood with extraction fans switched off. Gloves should be worn when
handling stock solution and more dilute solutions containing PMSF.
Do not dispense ethanol in the presence of a source of ignition, e.g. naked flame.
PMSF should be stored in the “toxic” cupboard.
Ethanol should be stored in the solvents cupboard.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
None
10
MONITORING
Performance of control measures,
None
Personal exposure
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
PMSF: Dispose of as “Special Waste” via Estates Office. Rinse empty container with copious amounts of water before
disposal.
Ethanol: Wash small amounts (<100 ml) down the sink with lots of water or allow to evaporate in fume hood. Larger
Amounts should be disposed of to laboratory’s general non-halogenated waste container.
100 ml PMSF solution: Dispose of to laboratory’s general non-halogenated waste container.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
5 years.
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13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
PMSF (solid): Evacuate area. Wearing rubber gloves, eye protection and face mask sweep up and place in a labelled
bag for waste disposal. Avoid breathing and raising dust, eg. by dampening material with water.
Ethanol: Shut off or remove possible sources of ignition. Soak up spill with inert spill absorbent (e.g. paper towels).
Ventilate affected area.
100 mM PMSF solution: Shut off or remove possible sources of ignition. Soak up spill on inert spill absorbent, e.g. paper
towel.
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
PMSF
Skin contact: Drench the affected area with clean running water. Remove contaminated clothing and shoes and wash
before re-use. Apply calcium gluconate gel on and around affected area (do NOT allow contact with eyes) and in severe
cases transfer casualty to hospital. Continue to massage gel for at least 15 minutes, and until pain is relieved. The affected
area may be covered with a dressing soaked in gel and bandaged lightly.
Eye Contact: Flush the eye with clean running water for at least 20minutes (continue during transfer to hospital). Transfer
casualty to hospital.
Ingestion: Do NOT make the casualty vomit. Wash out mouth with water. If conscious, give 0.5 litre of water to drink.
Obtain medical attention.
Inhalation: Remove from exposure and transfer to hospital. If necessary seek help of a First Aider to resuscitate casualty.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
PMSF (solid): Transfer spilled material and leaking container to suitably labelled container. Avoid generating dust, dampen
spillage with water and scoop up. Thoroughly wash contaminated area with lots of water.
Ethanol: Soak up spill with paper tissues. If a large volume is spilt, allow ethanol to evaporate in fume hood before
Placing tissues in disposal bin.
100 mM PMSF solution: Soak up spill with paper tissue. Wash affected area with lots of water.
CONTACT
PHONE
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.