Wolfson Centre for Inherited Neuromuscular Disease
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Product Datasheet for
Monoclonal Antibody
Name: MANCHO10 - MOUSE ANTI- Utrophin
Clone No. 9E10
Specificity: Utrophin
Species: human, mouse (not fish, not xenopus)
Isotype: Ig G1
Western blot: Stains 400 kD band in Hela total protein extract. Dilution: 1/50
Double labeling of normal human muscle
MANCHO10(9E10) / UTROPHIN
OVERLAY
BUNGAROTOXIN /
NEUROMUSCULAR JUNCTIONS
Immunolocalization: Sarcolemma of fibres in Duchenne muscular dystrophy.
Blood vessels, nerves and neuromuasular junctions.
Dilution:1/4
Immunogen: Fusion Protein: The last 329 amino-acids of utrophin linked to -galactosidase
Epitope mapping: Amino-acids 3164-3184 by phage-display
Storage recommendation: Store one month's use at 4oC and remainder in aliquots at –20oC.
Stable for at least 12 months under these conditions
Supplied as liquid culture supernatant in DMEM/10% horse serum (contains AZIDE: toxic!)
(Identical culture medium can be supplied as negative control)
Reference: An epitope structure for the C-terminal domain of dystrophin and
utrophin. G.E. Morris, S. G. Sedgwick, J. M. Ellis, A. Pereboev, J. S. Chamberlain and
Nguyen thi Man Biochemistry 37 (1998) 11117-11127