EY 693 Project Proposal Using Bayesian Statistics to Develop Paleoindian Chronologies on the Great Plains, North America Lucy Burris 4/06/2007 Project Summary Accurate and reliable chronologies are important to any study of historic processes. Accurate chronologies are especially important for answering questions of population dynamics, resource use, and cultural response to change in the absence of written or oral records. In this proposal, Bayesian statistical methods will be used to refine the existing chronologies of the North American Great Plains Paleoindian (13,000 - 8,000 years ago) period. Within this period at least 16 different cultural complexes have been identified. Dates of these complexes have been established by ad hoc analysis of the roughly 250 radiocarbon dates available from archaeological sites assigned to this area and time period. Available site information also includes geographic location, artifact and faunal assemblage lists, and occasionally paleoenvironmental reconstructions. Bayesian methods show particular promise for data sets like this since they can be applied to small data sets, they allow the use of auxiliary information, and they permit reevaluation as new data is obtained. Application of Bayesian methods is expected to improve the resolution of the time ranges of the various cultural complexes, to identify where additional dating information is required, and to potentially, allow reevaluation of site cultural assignment when available evidence is problematic. EY693 - Burris Proposal 4/6/2007 Page 1 of 16 Project Description Many of us are intrigued by our past. We trace our genealogies; we participate in Civil War re-enactments. We go to museums to find out about King Tut and Lewis and Clark. We haunt flea markets, thrift stores, and estate auctions to find treasures of the past. All these activities are reasonably well informed by written records and first hand accounts. We know with a fair amount of certainty when events occured and when and where cultures existed and individuals lived. We can build reasonably good chronologies and spatial maps because we have a lot of information at hand. But as the past becomes further removed in time few or no written records exist, first hand accounts are lost or garbled and artifacts become objects without information. One of the goals of archaeology is to build our knowledge of this lost past, to understand what cultures came before, how those people lived, and why they vanished. Without written records chronology building relies upon indirect evidence such as tree-ring and radiocarbon dating, diagnostic artifacts, and stratigraphy. Using the sparse data available and a technique called Bayesian statistics, this proposal intends to refine the chronologies of the ancient past in North America so we can better answer the questions of how early people lived, where they came from, and why they disappeared. We know that anatomically modern humans developed in Africa and dispersed around the globe several hundreds of thousands of years ago. We think that people came to the Americas only within the last 20,000 years or so. This is subject to intense debate as just a handful of archaeological sites have been radiocarbon dated to this early date. Only since 1927 with the discovery of a "Folsom" projectile point embedded in a extinct bison bone at Folsom, New Mexico, have we known that people have been in the Americas for more than a few thousand years or so. We now know that a variety of different cultures - or at least a variety of different tool technologies - occupied the Americas for several thousand years beginning at the end of the Pleistocene (13,00012,000 years ago). What is less clear is how these cultures (or technologies) relate to each other through time and space. Were they coincident, were they sequential, did one always precede another? EY693 - Burris Proposal 4/6/2007 Page 2 of 16 Why do we care about these relationships of the distant past (see for example Kuzmin and Keates 2005)? Understanding when, how long, and where a culture developed, fluoresced, and disappeared can tell us how they used the resources around them and knowing how those resources changed can help us understand how humans have modified their environments and why a culture may have disappeared (or changed into a form no longer recognized as the same). If we know cultures developed over a short period of time over a large spatial area we can suspect that different patterns of development took place compared to slower development in a smaller area. A good example is the Clovis culture which produced distinctive projectile points made of high quality stone with a hafting flute. These points are found across North and northern South America. At one time, it was thought that a small group of Clovis people arrived from Asia and through population growth and migration populated the Americas. This seems reasonable if the culture lasted for one or two thousand years. Recent re-dating of Clovis sites has suggested that the sites were formed within about a 200 year window (Waters and Stafford 2007). This sheds doubt on either the small group idea or on the idea of moderate population growth or on idea of migration. Other explanations for the short time but wide geographic spread of Clovis include a large initial migration into the Americas, hyper population growth, or technology transfer to indigenous people rather than dispersion of Clovis people across the continent. Clearly these alternatives lead to very different understanding of early cultures. Having "robust and reliable chronologies" is essential to any investigation of the past (Buck and Millard 2004). This proposal will revisit the existing chronologies of the North American Great Plains during the Paleoindian period, roughly 13,000 - 8,000 years ago (see Appendix A for a full listing of cultures and dates used in this proposal). Chronology building during this period is important to understanding the peopling of the Americas both in the variety of cultures initially present and in how they used and modified the landscapes around them and in understanding why they disappeared. Building chronologies during this period is difficult due to the scarcity and poor condition of sites, the scarcity and poor condition of suitable material for radiocarbon EY693 - Burris Proposal 4/6/2007 Page 3 of 16 dating (primarily bone and charcoal but also shell and plant materials), the scarcity of culturally distinct artifacts (primarily projectile points), and the lack of precise calibration curves with which to convert radiocarbon dates into calendar dates. Compounding these problems, many radiocarbon dates that were obtained from sites excavated between 1950 and 1970 are problematic due to field and lab issues (Baillie 1990). In many cases obtaining new sample material is impossible since the sites were destroyed at the time of excavation. The North American Great Plains provides a useful geographic area within which to test a Bayesian approach to chronology building. In this proposal the Great Plains are taken to extend from southern Alberta, Canada to the Rio Grande in southern Texas and from the Rocky Mountains to the tall-grass prairies of Kansas and Nebraska (Figure 1). Although sites are scarce, relative to other areas of North America they are welldispersed and wide spread, have good preservation, and represent a continuous occupation from earliest times through the present day. Subsistence patterns during the Paleoindian period were all nomadic hunting and gathering using stone and bone tool technologies - a subsistence pattern that leaves a consistent archaeological record (discounting preservation issues) across time. Sites types generally include short and long term camps, kill sites, lithic quarries and workshops, burials, and tool caches. Sites in the Great Plains include the original Folsom site (described below), the late Pleistocene Clovis site at Blackwater Draw in New Mexico, and the extensive Lindenmeier Folsom camp site in Colorado. Finally, chronologies have been developed against which the methods proposed here can be evaluated. The relatively recent availability of personal computers capable of solving complex computational problems, the availability of software for Bayesian computation (for example WinBugs), and recent interest in paleochronologies with the discussion of "Kennewick Man's" antiquity and connection to modern tribes makes this an opportune time to apply Bayesian methods to chronology determinations (see Bruning 2006 for a discussion of the legal complexities of the Kennewick remains). EY693 - Burris Proposal 4/6/2007 Page 4 of 16 Figure 1. Great Plains as defined by Holliday (2001). Numbers indicate major Paleoindian sites in the area. Background Since the 1950s archaeologists have used radiocarbon dating to establish ages (radiocarbon determinations) and associated calendar dates for excavated sites. A more detailed description of radiocarbon dating is available in Appendix B. Combined with artifact assemblages, these dates have been used to assign sites to cultural groups. With enough site assignments cultural chronologies - the earliest start and end dates for a culture - have been established. Dates have been scarce and a variety of techniques have been used in the past to distinguish good from bad dates, to average multiple determinations, and to process dateable material. The scarcity of data has precluded the definition of precise geographic ranges for cultures and has sometimes caused conflicting interpretations of cultural assignment, particularly when artifact definitions are not EY693 - Burris Proposal 4/6/2007 Page 5 of 16 properly applied (see for example Sellet 2001). Cultures which are currently problematic are the Goshen complex which in some areas seems to be concurrent with Clovis and in others to post-date Folsom (which in most areas are sequential, see discussion in Frison 1990), the Cody complex which appears across wide areas of the Plains but seems to have many manifestations, and Midland whose current time frame looks much like Folsom and whose artifacts look like Folsom projectile points without a diagnostic flute. Archaeologists are asking if Goshen was really contemporary with Clovis, if Cody is a single culture or a hybridization, and if Midland is a variant of Folsom. A goal of this proposal is to attempt to resolve these types of issues in the current chronology. Radiocarbon dating revolutionized our ability to build absolute chronologies of cultural groups from material collected in archaeological excavations. Initially, obtaining dates was an expensive and destructive process, limiting the number of samples submitted to a minimum. While still destructive and expensive, modern dating techniques are far more accurate and require only milligrams rather than grams of material. Sample processing has improved so that issues with marine contamination, bone collagen, and application of calibration curves have been reduced. Statistical analysis techniques have also advanced so that the potential exists to use multiple lines of evidence including stratigraphy, artifact presence, and paleoenvironmental data to refine dating results. Unfortunately, since archaeology is a destructive process, many sites can not be redated using modern methods as the sites no longer exist and materials may not have been preserved. Revising chronologies can require the use of preexisting information, although it may be problematic. Several large data sets have been compiled of radiocarbon dates from the North American Great Plains during the Paleoindian period. Meltzer (2006) has compiled all the radiocarbon dates for the Folsom site in New Mexico - dates span a collection period from the 1970s through the 1990s. Waters and Stafford (2007) as already mentioned have recently evaluated a set of sites that are assigned to the Clovis period and obtained new radiocarbon dates for questionable sites. Holliday (2000) and Eighmy and LaBelle (1996) have recently compiled chronologies of the North American Great Plains EY693 - Burris Proposal 4/6/2007 Page 6 of 16 Paleoindian cultures from Clovis to Cody (approximately 8,000 years ago). These chronologies include uncertainty about culture beginning and end dates, geographic dispersion, and suggest some inconsistencies where cultures may not always follow the came sequence in time. All of these compilations rely upon current best practice (generally ad hoc decisions) to determine the validity of radiocarbon dates to include in their reporting. Because of the recentness of the Meltzer and Waters and Stafford evaluations and redating, both offer valuable "truth" data sets due with which to compare the method proposed here. The research proposed here intends to refine the work of Holliday (2000) and Eighmy and LaBelle (1996) by using Bayesian statistics to include/exclude dates based on geographic location, site stratigraphy, date determination history, and artifact assemblage composition, to establish a potentially stronger chronology of the area and period. The data sets of Meltzer (2006) from Folsom and Waters and Stafford (2007) from the Clovis period will be used as reference data sets to test and develop the method. The work is also expected to determine the overall validity of the cultural assignments of both sites to cultures and cultures as meaningful elements in time and space. The work will also identify specific sites / time frames for which additional radiocarbon determinations should be obtained. If the Bayesian approach is successful, the chronology developed by this method can continue to be refined as new dates become available. Bayesian statistics has been successfully applied to many problems of radiocarbon dating, particularly, the calibration of radiocarbon determinations to obtain calendar dates (Buck et al. 1996). Ox-Cal the primary tool for converting radiocarbon ages to calendar dates is based on a Bayesian framework and provides a probability range for the calendar dates of a given radiocarbon age (or set of ranges). Bayesian statistics has been used on a smaller scale than proposed here to determine an absolute chronology for the JamaCoaque tradition in western Ecuador (Zeidler et al. 1998). The method has also been successfully applied to the determining the likelihood of a given ship wreck on the Great EY693 - Burris Proposal 4/6/2007 Page 7 of 16 Lakes being a particular vessel based on ship size, propulsion method, cargo, and routing (O'Shea 2004). Experimental Design This effort will be conducted in three phases comprised of two pilot studies and a final product. The pilot studies will be used to develop and demonstrate the methods in preparation for the final product. The first pilot study ("Folsom Pilot) will use published radiocarbon dates from the well-studied Folsom site, New Mexico. In 1927, this site was the first to explicitly demonstrate the co-existence of humans and Pleistocene fauna in North America (Meltzer 2006). Primary excavation was conducted in the 1920s with a revisit to the site in the 1930s to collect material for tree-ring dating which was submitted for radiocarbon dating to Willard Libby in 1949 (Meltzer 2006:7). These dating attempts failed and the another visit was conducted in the late 1970s to collect data for paleoenvironmental reconstruction and new radiocarbon dating. The site was revisited for a third time in the late 1990s to resolve questions left unanswered by previous work. In total roughly 50 radiocarbon determinations (both accepted and rejected) from well documented and ordered stratigraphy have been obtained from the site. This data set will be used to test for the best form the prior distributions (for example, assuming that dates are drawn from a uniform distribution on an interval from A to B), to test for the ability to separate acceptable from reject dates, and to determine the computational tractability of the problem. The second pilot study ("Clovis Pilot") will use a recently developed set of radiocarbon dates from the Clovis period in conjunction with earlier dates for theses sites (Waters and Stafford 2007; and others). In 2007, Waters and Stafford completed a project to establish accurate radiocarbon determinations for 30 possible Clovis sites. In addition to re-evaluating existing determinations, they obtained 16 new determination using stateof-the-art techniques. They were able to exclude some dates from the Clovis period and to improve the resolution on the Clovis culture's time horizon to roughly 200 years. Clovis Pilot will endeavor to replicate the Waters and Stafford work and establish both the date range and the accept/reject determinations for the 150 dates they considered. EY693 - Burris Proposal 4/6/2007 Page 8 of 16 While Folsom Pilot is a "within site and within culture" study, Clovis Pilot is "across sites and within culture." The Final Product will use all dates in Holliday (approximately 250 dates [2000]) and Eighmy and LaBelle (1996) plus any new dates (for example those in Meltzer 2006 and Waters and Stafford (2007) that can be obtained for Paleoindian age sites on the Great Plains including geography, stratigraphy, paleoenvironmental factors and artifact assemblages. Based on the methods developed during Folsom and Clovis Pilots, this phase will develop a set of new chronologies based on accept/reject criteria. Finally, using paleoenvironmental data (if sufficient data is available), the chorology will be revisited to identify patterns based on landscape use. Compared to Folsom and Clovis Pilots, the Final Product is "across sites and across culture." If time permits an additional analysis (Final Product B) will build a culture-free chronology and then attempt to identify pseudo-cultural boundaries. The data set will be modified by removing the cultural assignment of all the sites and using the knowledge of geography, site date, and artifact and faunal assemblage to determine the probability that a site (or cluster of sites) belong to a given a priori culture or if there are "natural" groupings of sites which suggests currently unknown cultures. If unknown cultural groupings emerge, this could suggest that technologies are merely tools and do not carry as significant a cultural connotation as is currently assumed. Methods As described above the proposal will be executed in several stages with the results of each stage providing a basis for the following stage. A failure to get suitable results in a given stage will result in a reevaluation of the previous stage results and examination of the assumptions and theory for the failed stage. Figure 2 provides a sketch of the proposed process flow. Steps are described in more detail below. Since this is an investigative proposal, the methods are still being developed and will be refined as new insight is obtained. EY693 - Burris Proposal 4/6/2007 Page 9 of 16 2. 1. Explore Software Tools Acquire data Select/learn Tool 3. Potential Prior Distributions Build Database Build Models Increasing complexity Final Product / B Clovis Pilot Folsom Pilot Refine/ Validate 4. - 7. 8. Publish Results Figure 2. Conceptual diagram of methodology. 1. Acquire data from sites - location (latitude, longitude, elevation), all radiocarbon determinations (value, lab date, lab, source material, stratigraphic position, status), artifact list, paleoenvironmental information, stratigraphic description, faunal evidence and build database an information database (in Microsoft Access or Excel, GIS ready). 2. Evaluate potential software tools WinBugs (freeware), Ox-cal (on-line tool), MSBNx (Microsoft freeware used by O'Shea [2004]), select best, and become proficient in it. WinBugs is a command line / execution script program which allows many forms of continuous distributional assumptions, its ability to handle discrete distributions is less clear. MSBNx, in contrast, handles discrete distributions well (as O'Shea [2004] used it, he assigned discrete probabilities to the chance of a ship being say wind or steam powered). The ability of MSBNx to handle continuous distributions is unknown. Ox-Cal is reported to have the ability to include stratigraphic relationships but whether this EY693 - Burris Proposal 4/6/2007 Page 10 of 16 extends beyond a single site and whether any other type of auxiliary information can be included is also unknown. 3. Compile a candidate list of potential priors based on literature review, other examples, and current best practice. Ox-Cal apparently uses a uniform prior and this is the prior most often suggested in the relevant literature. This may be more due to convenience than any theoretical basis and this is an area requiring additional research. 4. Build and evaluate Folsom Pilot using uniform prior ("no information"), modify with other priors from Step 3. Constrain based on stratigraphy relationships. Goodness of fit test to be determined (Bayesian Information Criterion - BIC, deviance, etc). Revise model as needed. Compare to results published by Meltzer (2006). 5. Build and evaluate Clovis Pilot using prior model from Folsom Pilot. Constrain or condition based on diagnostic artifact/faunal presence / absence. Revise model as needed. Compare results to those published by Waters and Stafford (2007). 6. Build and evaluate Final Product. Constrain based on geography, diagnostic artifacts, /faunal presence/absence. Use prior from Clovis and Folsom Pilots. Revise model as needed. Modify model with conditioning on distance to water, elevation, distance to lithic sources, etc. 7. Build a "culture" free model (Final Product B) to assign a "cultural probability vector" to sites. (similar to assigning ship names to wrecks on the Great Lakes, O'Shea [2004]) 8. Publish results. Since this is an investigative proposal, there is no guarantee of success. Potential pitfalls include the problem that the data and analyses are self-referential and the computational complexity of the data set. The same data that is used to build the models EY693 - Burris Proposal 4/6/2007 Page 11 of 16 will also be used to test them. Potentially, once a base model is established, the input data can be partitioned (if there is enough of it) and one part used to build the model and the remainder used to test. A bootstrap approach may also be valid. The second problem of computational complexity is, at this point, an unknown. WinBugs for instance uses a Monte Carlo Markov Chain simulation approach to obtain a solution. The Gibbs sampler which it uses is fairly efficient and yet in the Final Product, over 250 dates need to be analyzed. Potential solutions include using a computer with a dual core processor and possibly using distributed computing methods (running the computation on multiple machines at once). At least one additional problem may arise and that is during the data collection phase, the required raw data (radiocarbon determinations rather than calendar dates) may not be obtainable. Dates discarded prior to publication (those discarded by the principal investigator) may be impossible to recover. Every effort will be made to acquire this information, but particularly for older dates, it may simply not be available. In this case the dates that are available will be used. Potentially a set of "pseudo-dates" can be constructed to represent lost dates and used to test model robustness. Expected Results It is anticipated that the methods described above will provide chronologies similar to published results for both the Folsom and Clovis Pilots. These results will provide confidence that the method is sound when applied to a data set without a supported reference. The Final Product is anticipated to provide a tighter chronological range for existing cultural partitioning and potentially will suggest that some cultural partitioning is poorly constructed. If Final Product B is produced, it should be especially insightful into the validity of current cultural partitioning. The Final Product will clearly indicate where, if possible, additional radiocarbon determinations should be obtained and which date ranges have less clarity compared to other date ranges. Broader Impacts of the Study The research developed here will be used by archaeologists to establish appropriate site time ranges and as an integral part of research designs. The chronologies developed here will become prior information for future refinement of the chronology as EY693 - Burris Proposal 4/6/2007 Page 12 of 16 more dates are obtained (one of the values of a Bayesian approach). Gaps in the developed chronologies will provide a basis for collecting additional dates to further refine the timeline where data is scarce. The work will also illustrate benefits to be obtained by examining old data. Radiocarbon dating is still an expensive process and the current work will show that old dates that were previously discounted may be salvageable. At least three publications are anticipated: A Folsom Pilot/Clovis Pilot demonstration paper, a Final Product - chronology paper, and a use of paleoenvironmental data to explain cultural disappearance paper. Two additional papers will depend upon funding and analysis results. If Final Product B demonstrates that there are a new unknown cultural groups, this will be a publishable result. Depending upon funding availability, a final paper will use specifically acquired radiocarbon determinations to fill in gaps in the Final Product chronology. The results and methods will be made publicly available through the CSU Anthropology Department Paleoindian web site which is currently under development. References Baillie, M. G. L. 1990 Checking Back on Assemblage of Published Radiocarbon Dates. Radiocarbon 32(3):61-366. Bruning, Susan B. 2006 Complex Legal Legacies: The Native American Graves Protection and Repatriation Act, Scientific Study, and Kennewick Man. American Antiquity 71(3):501-521. Buck, Caitlin E., William G. Cavanagh, and Clifford D. Litton 1996 Bayesian Approach to Interpreting Archaeological Data. John Wiley and Sons, New York. Buck, Caitlin E., and Andrew R. Millard, editors 2004 Bayesian Chorological Data Interpretation: Where Now? In Tools for Constructing Chronologies: Crossing Disciplinary Boundaries, edited by Caitlin E. Buck and Andrew R. Millard, pp. 1-24. Springer, New York. Eighmy, Jeffrey, and Jason M. LaBelle 1996 Radiocarbon Dating of Twenty-seven Plains Complexes and Phases. Plains Anthropologist 41:53-69. Frison, George EY693 - Burris Proposal 4/6/2007 Page 13 of 16 1990 Clovis-Folsom-Goshen Relationships in the Northern High Plains. In Megafauna and Man: Discovery of America's Heartland, edited by Larry D. Agenbroad, Jim I. Mead, and Lisa W. Nelson, pp. 100-108. The Mammoth Hot Springs, South Dakota, Inc. Scientific Papers, Volume 1, Hot Springs South Dakota. Holliday, Vance T. 2000 The Evolution of Paleoindian Geochronology and Typology on the Great Plains. Geoarchaeology 15(3):227-280. Kuzmin, Yaroslav V., and Susan G. Keates 2005 Dates are not Just Data: Paleolithic Settlement Patterns in Siberia Derived from Radiocarbon Records. American Antiquity 70(4):773-789. Meltzer, David J. 2006 Folsom: New Archaeological Investigations of a Classic Paleoindian Bison Kill. University of California Press, Berkeley. O'Shea, John M. 2004 The Identification of Shipwreck Sites: A Bayesian Approach. Journal of Archaeological Science 31:1533-1552. Renfrew, Colin, and Paul Bahn 1996 Archaeology: Theory, Methods, and Practice, 2nd edition. Thames and Hudson, New York. Sellet, Frederic 2001 A Changing Perspective on Paleoindian Chronology and Typology: A View from the Northwestern Plains. Artic Anthropology 38(2):48-63. Taylor, R. E., C. Vance Haynes, Jr., and Minze Struiver 1996 Clovis and Folsom Age Estimates: Stratigraphic Context and Radiocarbon Calibration. Antiquity 70:515-525. Waters, Michael R., and Thomas W. Stafford, Jr. 2007 Redefining the Age of Clovis: Implications for the Peopling of the Americas. Science 315:1122-1126. Zeidler, James A., Caitlin E. Buck, and Clifford D. Litton 1998 Integration of Archaeological Phase Information and Radiocarbon Results from the Jama River Valley, Ecuador: A Bayesian Approach. Latin American Antiquity 9(2):160-179. Budget No budget is required for the pilot studies as all data and software products are available in the public domain. It is an expected result of the final product that there will EY693 - Burris Proposal 4/6/2007 Page 14 of 16 be "holes" or weak spots (time frames with multiple probable cultural assignments or with few dates) in the chronology. Obtaining and retesting approximately 25 samples is expected to be able to resolve these gaps. Retesting each sample is expected to cost ~$1500 (including sample procurement, prep, and testing) for a budget need of $37,500. Appendix A General Chronology and Culture Groups on the North American Great Plains, between 13,000- 8,000 years ago (adapted from Holliday 2000). Cultural Group Clovis Folsom Goshen Midland Northern Plains 11,200 - 10,900 10,900 - 10,200 still unclear still unclear Plainview Agate Basin Dalton Milnesand / Lubbock Lake 10,000 ± 200 10,500 - 10,000 Southern Plains 11,600 - 11,000 10,900 - 10,100 Eastern Plains 10,000 ± 500 9950 Hell Gap Alberta /Alberta-Cody Classic Cody Firstview 10,000 ± 500 10,200 - 9400 9400 - 8800 Fredrick, Jimmy Allen, Angostura Golondrina St. Mary's Hall Texas Angostura 9400 - 7800 9400 - 8200 9200 - 8900 9500 - 8500 8800 - 8000 Dates are the start and end times in radiocarbon years before present (where present is 1950 A.D.) If not enough dates are available for a start and end range, a center point with one standard deviation is given. Because of non-linearities in the radio carbon calibration curve, a rough rule of thumb for BP dates around 10,000 years is 10,000 BP (radiocarbon date) ~ 10,000 BC (calendar date) ~ 12,000 calendar years BP. This relationship is less reliable as dates become younger, note that 0 BP ~ 1950 AD ~ 2000 calendar years BP. EY693 - Burris Proposal 4/6/2007 Page 15 of 16 Appendix B Radiocarbon Dating Radiocarbon dating measures the ratio of carbon 14 (14C) to carbon 12 (12C) in a sample (Renfrew 1996). When a living organism dies it contains a 14C / 12C ratio that matches (with some caveats) the environment in which it lived. Over time, the unstable 14 C decays to 12C changing the ratio of these molecules. Knowing the decay rate and working backward from the current ratio, the time since death as a function of decay rate (age) can be determined. While the ratio of molecules is relatively easy to measure using accelerated mass spectrometry (AMS), determination a calendar date given an age is more difficult. First the 14C / 12C ratio of the environmental pool is not been constant over time, so instead of a mathematical relationship between carbon ratio and age, an empirical calibration curve has been developed based on tree-rings, lake varves, and coral dating. Second, terrestrial and marine carbon pool ratios appear to be different at given points in time and there may even be differences in terrestrial pools between North and South America, thus a given date needs to be conditioned based on the carbon pool or combination of carbon pools most likely to have contributed to organism stored carbon. Third, prior to AMS dating in the 1980s, many grams of material were required for dating. To obtain adequate sample material from some sites, charcoal was collected from several locations with the possibility that although samples were not contemporary in age they were commingled for testing resulting in a average date for all the materials. While this problem has been eliminated with AMS dating which uses only milligrams of material, older dates run this risk. Forth, extraction of datable carbon from bone material has been problematic and resulted in incorrect dates (Taylor et al. 1996). Fifth, radiocarbon dating is useful only to about 40,000 years ago; as samples get older the ability to measure the number of 14C atoms was problematic prior to AMS dating (which measures the atoms not the decay rate). Finally, it is possible for cultural materials to be altered by carbon uptake from the surrounding sediments, say leaching from a nearby coal seam, although this is thought to be rare and generally detectable. Calibration curve improvements are particularly important. Although the practice has been inconsistent, researchers should always publish both the original radiocarbon determination (giving a BP or rcyBP designation) and the current best calibration dates (expressed in BC/AD or calibrated yr BP). This way if the calibration curve changes (as occured in the mid-1990s), new calibration dates can be determined. When only the calibrated dates are published (which is not uncommon since these are dates that are comparable in a temporal sense), there is no possibility of reevaluating them in light of new information. AMS dates for the period from 10,000 - 20,000 years ago, have a standard deviation of about 100 years.(Kuzmin and Keates 2005). EY693 - Burris Proposal 4/6/2007 Page 16 of 16