Supplementary figure 1: Denaturing gradient gel electrophoresis of the PCR
amplified V2/V3 region of 16S rRNA genes from the caecal fluid of horse 1. The left
and right gels were loaded with 1 and 6 l of PCR product respectively. Numbers at
the top of the gel indicate time, in hours post-oligofructose administration (POA), and
5
L = ladder. Horse 1 initially exhibited histological evidence of laminitis at 12 h POA
and clinical signs of laminitis at 28 h POA.
Supplementary figure 2: Denaturing gradient gel electrophoresis of the PCR
amplified V2/V3 region of 16S rRNA genes from the caecal fluid of horse 2. The left
10
and right gels were loaded with 1 and 6 l of PCR product respectively. Numbers at
the top of the gel indicate time, in hours post-oligofructose administration (POA), and
L = ladder. Horse 1 initially exhibited histological evidence of laminitis at 30 h POA
and clinical signs of laminitis at 38 h POA.
15
Supplementary figure 3: Denaturing gradient gel electrophoresis of the PCR
amplified V2/V3 region of 16S rRNA genes from the caecal fluid of horse 1. The left
and right gels were loaded with 1 and 6 l of PCR product respectively. Numbers at
the top of the gel indicate time, in hours post-oligofructose administration (POA), and
L = ladder. Horse 1 initially exhibited histological evidence of laminitis at 30 h POA
20
and clinical signs of laminitis at 43 h POA.
Supplementary figure 4: Denaturing gradient gel electrophoresis of the PCR
amplified V2/V3 region of 16S rRNA genes from the caecal fluid of horse 1. The left
and right gels were loaded with 1 and 6 l of PCR product respectively. Numbers at
25
the top of the gel indicate time, in hours post-oligofructose administration (POA), and
L = ladder. Horse 1 initially exhibited histological evidence of laminitis at 12 h POA
and clinical signs of laminitis at 20 h POA.
Supplementary figure 5: Denaturing gradient gel electrophoresis of the PCR
30
amplified V2/V3 region of 16S rRNA genes from the caecal fluid of horse 1. The left
and right gels were loaded with 1 and 6 l of PCR product respectively. Numbers at
the top of the gel indicate time, in hours post-oligofructose administration (POA), and
L = ladder. Horse 1 initially exhibited histological evidence of laminitis at 18 h POA
and clinical signs of laminitis at 26 h POA.
35
Supplementary table 1: Raw qPCR and FISH data presented in this publication.
FISH data are expressed as relative abundances, expressed as a percentage of the total
EUBMIX-hybridising organisms. Subjective scores were: 0%, occasional presence
(fewer than one microorganism per field of vision and not possible to quantify), <1%,
40
1-5%, 6-10%, and then in 10% increments up to 91-100%.