January 28, 2005 Multiple choice questions (numbers in brackets indicate the number of correct answers) DNA was first isolated in the 17th century 18th century 19th century 20th century Components of DNA are Phosphate, ribose, pyridines, and purines Phosphate, ribulose, and nucleotides Sulfate, ribose, pyrimidines, and purines Phosphate and nucleotides Phosphate, deoxyribose, pyrimidines, and purines (1) (1) The DNA double helix is stabilized by Ionic bonds Covalent bonds Hydrogen bonds Hydrophobic interactions (2) DNA is synthesized in 5’->3’ direction 3’->5’ direction in both directions (1) The major form of DNA in cells is A-DNA B-DNA C-DNA Z-DNA (1) Z-DNA winds to the left is thicker than B-DNA has a flat major groove has a broad minor groove (2) Sizes of genomes of free-living organisms have been found to range from approximately 2-200 Mbp 0.5-1,000 Mbp 100-2,000,000 Mbp 1,000-1,000,000,000 Mbp 0.5-200,000 Mbp [1 Mbp = 1 million bp] (1) The size of the human genome is about 0.5 Mbp 10 Mbp 200 Mbp 3,000 Mbp 40,000 Mbp 500,000 Mbp (1) Most sequences in the human genome belong to Genes Pseudogenes Gene fragments Interspersed repeats Tandem repeats (1) Which of the following genomes is richest in interspersed repeat sequences? Drosophila genome Human genome Maize genome Saccharomyces genome E. coli genome (1) Which genome has the fewest introns? Drosophila genome Human genome Maize genome Saccharomyces genome E. coli genome (1) A nucleosome consists of Chromatin and nucleotides Chromatin and histones DNA and chromatin DNA and histones Nucleoids and histones (1) Centromers contain Repeated DNA Chromatids Telomeres Proteins Microtubules Genes (3) Gene density can be high in telomeres anywhere on the chromosomes in centromeres in metaphase chromosomes in anaphase chromosomes (1) Self study questions at the end of chapters 1 and 2 of the GENOMES textbook: Chapter 1: 7., 10., 11., 14. Chapter 2: 1., 3., 10., 15.