彭國証 教授 Peng, Kou-Cheng
經歷：
1987-1989
The Wistar Institute, Philadelphia, Pennsylvania, USA
Research Assistant, Department of Biochemistry, Clemson University
The Wistar Institute, Philadelphia, Pennsylvania, USA
1989-1991
Teaching Assistant, Department of Biochemistry, Clemson University
The Wistar Institute, Philadelphia, Pennsylvania, USA
1992-1993
Postdoctoral Fellow, Unit of Protein Biochemistry
University of Georgia, Athens, Georgia, USA
1993-1995
Postdoctoral Fellow, Department of Biochemistry and Molecular Biology
University of Georgia, Athens, Georgia, USA
1995-1996
Postdoctoral Fellow, Department of Microbiology
Purzer Pharmaceutical Company, Taipei, Taiwan,
1996-1998
Senior Scientist, Department of Research and Development
INSERM, Paris, France,
1998-2000
Visiting Scientist, U478
AGT Biotechnology, Taiwan
2000-2001
Senior Scientist
Yuan Zu University, Taiwan
2001-2001
Assistant Professor
National Dong Hwa University
2001-2009
Institute of Biotechnology/Department of Life Science
Assistant Professor
National Dong Hwa University
2009-present
Institute of Biotechnology/Department of Life Science
Professor
更詳細的教師個人基本資料表（PDF 檔）
一、專業領域：
1.
2.
3.
Protein Biochemistry
Microbial Secretome
Molecules and Mechanisms involving Trichoderma harzianum and infected plant host
實驗室介紹
二、期刊論文：
1. Yi-Jen Hsieh, Chih-Jui Chang, Chin-Feng Wan, Chin-Piao Chen,Yann-Lii Leu *, Kou-Cheng Peng * Molecules 2013, 18, 1949-1962. (2013, Feb).
apoptosis-inducing effect of extract derived from the roots of Euphorbia formosana mediates caspase-dependent cell death via Fas and Mitochondrial Pathway in le
cells.Molecules, 18, 1949-1962. (SCI, 26/56, CHEMISTRY, ORGANIC). NSC 101-2313-B-259-001.本人為通訊作者.
2. Cheng CH, Yang CA, Peng KC*. (2012, Nov). Antagonism of Trichoderma harzianum ETS 323 on Botrytis cinerea Mycelium in Culture Conditions. Phytopa
102(11):1054-63. (SCI, 42/190, Plant Sciences).本人為通訊作者.
3. Marthandam Asokan S, Lin HS, Yang HH, Peng KC*. (2012, Oct). Trichoderma harzianum ETS 323 mediated resistance in Brassica oleracea var capitata to Rhiz
solani involves the novel expression of a glutathione S-transferase and a deoxycytidine deaminase.Journal of Agricultural and Food Chemistry, 60(43):10723-3
3/57, Agriculture, Multidisplinary).本人為通訊作者.
4. Lin YR, Lo CT, Liu SY*, Peng KC* (2012, Mar). Involvement of pachybasin and emodin in self-regulation of Trichoderma harzianum mycoparasitic coiling.Jo
Agricultural and Food Chemistry, 60(9):2123-8. (SCI, 3/57, Agriculture, Multidisplinary).本人為通訊作者.
5. Yang CA, Cheng CH, Lee JW, Lo CT, Liu SY, Peng KC* (2012, Mar). Monomeric L-amino acid oxidase-induced mitochondrial dysfunction in Rhizoctoni
reveals a novel antagonistic mechanism of Trichoderma harzianum ETS 323.Jouranl of Agricutural and Food Chemistry, 60(10):2464-71. (SCI, 3/57, Agr
Multidisplinary).本人為通訊作者.
6. Cheng CH, Yang CA, Liu SY, Lo CT, Peng KC* (2012, Jan). L-amino acid oxidase-induced apoptosis in filamentous Botrytis cinerea.Analytical Bioch
420(1):93-5. (SCI, 19/73, Chemistry, Analytical).本人為通訊作者.
7. Cheng CH, Yang CA, Liu SY, Lo CT, Huang HC, Liao FC, Peng KC* (2011, Sep). Cloning of A Novel L-Amino Acid Oxidase from Trichoderma harzianum E
and Bioactivity Analysis of Overexpressed L-Amino Acid Oxidase.Journal of Agricultural and Food Chemistry, 59(17):9142-9. (SCI, 3/57, Agr
Multidisplinary).本人為通訊作者.
8. Yang CA, Cheng CH, Liu SY, Lo CT, Lee JW, Peng KC* (2011, Sep). Identification of Antibacterial Mechanism of l-Amino Acid Oxidase Derived from Trich
harzianum ETS 323.FEBS Journal, 278(18):3381-94. (SCI, 89/290, Biochemistry & Molecular Biology).本人為通訊作者.
9. Roy T. W. Huang, K. C. Peng, * H. N. Shih, G. H. Lin, * T. F. Chang, S. J. Hsu, Tina S. T. Hsu and Ivan J. B. Lin* (2011, Jun). Antibiotic Properties of Etho
Functionalized Imidazolium Salts.Soft Matter, 2011(7) 8392-8400. (SCI, 27/134 Chemistry, Physical).本人為通訊作者.
10. Yang CA, Cheng CH, Lo CT, Liu SY, Lee JW, Peng KC* (2011, May). A Novel l-Amino Acid Oxidase from Trichoderma harzianum ETS 323 Associat
Antagonism of Rhizoctonia solani.Jouranl of Agricutural and Food Chemistry, 59(9):4519-26. (SCI, 3/57, Agriculture, Multidisplinary). NSC 99-2324-B-259-001.
通訊作者.
11. Liu SY, Shibu MA, Jhan HJ, Lo CT, Peng KC*. (2010, Oct). Purification and Characterization of Novel Glucanases from Trichoderma harzianum ETS 323..Jo
Agricutural and Food Chemistry, 58(19):10309-14. (SCI, 3/57, Agriculture, Multidisplinary). NSC 99-2324-B-259-001.本人為通訊作者.
12. Chiu YH, Hsieh YJ, Liao KW, Peng KC* (2010, Feb). Preferential promotion of apoptosis of monocytes by Lactobacillus casei rhamnosus soluble factors.
Nutrition, 29(1):131-40. (SCI, 13/74, Nutrition & Dietetics). NSC 98-2317-B-259-001.本人為通訊作者.
13. Liu SY, Feng PC, Lo CT, Peng KC* (2010, Feb). Plate assay for fungal enzymes using cellophane membranes.Analytical Biochemistry, 397(1):121-3. (SCI, 1
Biochemistry & Molecular Biology). NSC 98-2324-B-259-001.本人為通訊作者.
14. Yang HH, Yang SL, Peng KC, Lo CT, Liu SY* (2009, Sep). Induced proteome of Trichoderma harzianum by Botrytis cinerea.Mycological Research, 113(Pt 9)
(SCI, 5/24, Mycology). NSC 98-2317-B-259-001.
15. Liu SY, Lo CT, Shibu MA, Leu YL, Jen BY, Peng KC* (2009, Aug). Study on The Anthraquinones Separated From The Cultivation of Trichoderma harzianum
Th-R16 and Their Biologically Activity.Jouranl of Agricutural and Food Chemistry, 57(16):7288-92. (SCI, 3/57, Agriculture, Multidisplinary)
97-2317-B-259-001.本人為通訊作者.
16. Lin JS, Chiu YH, Lin NT, Chu CH, Huang KC, Liao KW, Peng KC* (2009, Feb). Different effects of probiotic species/strains on infections in preschool chil
double-blind, randomized, controlled study. .Vaccine, 27(7):1073-9. (SCI, 41/139, Immunology). NSC 97-2301-B-259-001.本人為通訊作者.
17. Shih-Bin Lin, Shan-He Chen and Kou-Cheng Peng*（2009 年 01 月）。Preparation of antibacterial chito-oligosaccharide by altering the degree of deacetyl
β-chitosan in a Trichoderma harzianum chitinase-hydrolysing process。Journal of the science of food and agriculture，89: 238-44。（SCI，10/57, Agr
Multidiscilinary）。NSC 96-2317-B-259-001。本人為通訊作者。
18. Tseng SC, Liu SY, Yang HH, Lo CT, Peng KC* (2008, Aug). Proteomic study of biocontrol mechanisms of Trichoderma harzianum ETS 323 in response to Rhiz
solani .Journal of Agricultural and Food Chemistry, 56(16):6914-22. (SCI, 3/57, Agriculture, Multidisplinary). NSC 96-2317-B-259-001.本人為通訊作者.
19. Liu SY, Lo CT, Chen C, Liu MY, Chen JH, Peng KC* (2007, Apr). Efficient isolation of anthraquinone-derivatives from Trichoderma harzianum ETS 323 .Jo
biochemical and biophysical methods (Journal of Proteomics), 10 期, P.391-P.395. (SCI, 12/72, Biochemiscal Research Methods). NSC 95-2317-B-259-001.本人
作者.
20. Wu TZ, Su CC, Chen LK, Yang HH, Tai DF, Peng KC* (2005, Nov). Piezoelectric immunochip for the detection of dengue fever in viremia phase. Biosens
Bioelectronics, 21(5):689-95. (SCI, 1/27, Electrochemistry). NSC 91-2323-B-259-002.本人為通訊作者.引用次數依據 Google scholar citations 20121214.
21. Yang WJ, Lai JF, Peng KC, Chiang HJ, Weng CN, Shiuan D (2005, Sep). Epitope mapping of Mycoplasma hyopneumoniae using phage displayed peptide libra
the immune responses of the selected phagotopes.Journal of Immunological Methods, 304(1-2):15-29. (SCI, Immunology, 100/139). NSC 90-2311-B-259-001.引
依據 Google scholar citations 20121214.
22. Peng KC, Huang HS, Tzeng YM, Liu SY (2005, Jan). Circular dichroism analysis of destruxins from Metarhizium anisopliae. Journal of biochemical and bio
methods (Journal of Proteomics), 62(1):41-50. (SCI, 12/72, Biochemical Research Methods). NSC 91-2311-B-259-001.
23. Vandewalle A*, Cluzeaud F, Peng KC, Bens M, Luchow A, Gunther W, Jentsch TJ. (2001, Feb). Tissue distribution and subcellular localization of the ClC-5
channel in rat intestinal cells .American journal of physiology : cell physiology, 280(2):C373-81. (SCI, 19/79, Physiology).
24. Wu MS*, Yang CW, Bens M, Peng KC, Yu HM, Vandewalle A. (2000, Oct). Cyclosporine stimulates Na+-K+-Cl- cotransport activity in cultured mouse medulla
ascending limb cells.Kidney Internatioanl, 58(4):1652-63. (SCI, 4/73, Urology & Nephrology).
25. Peng KC, Cluzeaud F, Bens M, Van Huyen JP, Wioland MA, Lacave R, Vandewalle A.* (1999, Jun). Tissue and cell distribution of the multidrug resistance-as
protein (MRP) in mouse intestine and kidney.Jouranl of Histochemistry & Cytochemistry, 47(6):757-68. (SCI, 109/181, Cell Biology).本人為第一作者.
26. Peng KC, Puett D, Brewer JM.* (1997, Jun). Homodimer formation by the individual subunits of bovine lutropin as determined by sedimentation equilibrium.Jo
Molecular Endocrinology, 47(6):757-68. (SCI, 40/122, Endocrunology & Metabolism).本人為第一作者.
27. Angelova K, Ergul A, Peng KC, Puett D.* (1997, Mar). Metabolism of endothelin-1 by neuroblastoma x glioma hybrid (NG108-15) cells.Neuroscience
225(1):1-4. (SCI, 170/244, Neurosciences).
28. Zhang N, Peng KC, Chen L, Puett D, Pierce M.* (1997, Feb). Circular dichroic spectroscopy of N-acetylglucosaminyltransferase V and its substrate interactions
of Biological Chemistry, 272(7):4225-9. (SCI, 66/290, Biochemsitry & Molecular Biology).
29. Bermek H, Peng KC, Angelova K, Ergul A, Puett D.* (1996, Oct). Endothelin degradation by vascular smooth muscle cells.Regulatory peptides, 66(3):155-6
41/79, Physiology).
30. Peng KC, Bousfield GR, Puett D, Ward DN.* (1996, Aug). Circular dichroic spectroscopy of Arg46-nicked ovine lutropin alpha and derived fragments.Journal of
chemistry, 15(6):547-52. (SCI).本人為第一作者.
31. DeSilva TM, Peng KC, Speicher KD, Speicher DW.* (1992, Oct). Analysis of human red cell spectrin tetramer (head-to-head) assembly using complementary u
peptides.Biochemistry, 31(44):10872-8. (SCI, 106/290, Biochemistry & Molecular Biology).
二、研討會論文：
1. Amino acid sequence analysis of a putative L-amino acid oxidase fromT. harzianumETS 323.Yi-Wei Cheng,Kou-Cheng Peng.2007 生化暨生醫年會.
2. Purification, Crystallization and Preliminary X-ray Crystallographic Analysis of Native Xylanase and Xylotriose Complex fromTrichoderma harzianumETS323.S
Liu, En-Hung Liu, Kun-Che Chang, Yuan-Lung Chiang, Ya-Ting Yang, Ying-Cheng Hsieh, Chaur-Tsuen Lo, Yi-Hung Lin,Yen-Chieh Huang, Hong-Hsian
Chun-Jung Chen,Kuo-Cheng Peng＊, and Ming-Yih Liu*.2007 Annual Meeting of American Microbiology Society.
3. Proteomic study of the biocontrol mechanisms ofTrichoderma harzianumETS 323 responding toRhizoctonia solani.Shih-Chi Tseng, Shu-Ying Liu, Hsueh-Hu
Chaur-Tsuen Lo andKou-Cheng Peng. 2008 Taiwan Proteomics Society Eastern International Conference, June 04-05, P18.
4. The proteomic study of the biocontrol mechanisms of Trichoderma harzianum ETS 323 responding to Botrytis cinerea .Siwy-Ling Yang, Shu-Ying Liu, Hsueh-Hu
andKou-Cheng Peng.2008 Taiwan Proteomics Society Eastern International Conference, June 04-05, P19.
5. 33 Celulases Induction Mechanism ofTrichoderma harzianumstrain ETS-323.H. Lin1, H. Yang2, S. Liu3,K. Peng1.2nd ASM Conference on Beneficial Microb
Diego, CA . October 12-16, 2008.
6. 44B TheLactobacilus casei rhamnosusSoluble Factors Prevent LPS-Induced Inflamation by Promoting Monocytes Apoptosis via Mitochondria Pathway.Y. C
Huang2, Y. Hsieh3, J. Lin4, K. Liao1,K. Peng5.2nd ASM Conference on Beneficial Microbes; San Diego, CA . October 12-16, 2008.
7. 60B An antifungal potency ofTrichodermaand anthraquinone derivatives fingerprint system. F. Tsai1, P. Cheng1, S. Liu2,K. Peng1.2nd ASM Conference on Be
Microbes; San Diego, CA . October 12-16, 2008.
8. 核研所 97 年度委託計畫成果發表會(3/20)-菌種最適培養條件評估及發酵製程放大研究纖維酒精之高活性纖維素水解酵素開發張晉源 1、邱奕榮
昆鉦 1、林資翔 1、林佩榕 2、林宏勳 3、施慧儂 3、周俊伯 3、陳麗純 4、黃彥杰 4、Phimonphan Chuankhayan4 楊裕雄 1、袁俊傑 1、吳東昆 1、羅朝村
國証 3、陳俊榮 4、逄筱芳 5、劉明毅 5、門立中 5、黃文松 5.1 交通大學、2 虎尾科技大學、3 東華大學、4 同步輻射中心、5 核能研究所
9. P904 Isolation of Antifungal Metabolites ofTrichoderma.施慧儂、彭國証.The 25thJoint Annual Conference of Biomedical Science.
10. P672 Function of Novel Sm1 ofTrichderma harzianum.謝承翰、彭國証.The 25thJoint Annual Conference of Biomedical Science.
11. Characterization and overexpression ofL-amino acid oxidase fromTrichoderma harzianum ETS-323.C. A. Yang (1), C. H. Cheng (2), S.Y. Liu (3),K. C. Peng(
CPS Annual Meeting, Vancouver, Canada.
12. Purification and characterization of novel glucanases fromTrichoderma harzianumETS.Shu-Ying Liua*, Hua-Jing Jhanb, Chung-Wei Chena,Kou-Cheng Pengb20
Annual Meeting, Vancouver, Canada.
13. Self-upregulation of 3-methyl anthroquinones toTrichoderma harzianummycoparasitic coiling. Yi-Ruu Lin1*, Shu-Ying Liu2, Chaur-Tsuen Lo3, Kou-Cheng Peng
19, 2011, Taipei, ROC. The 26thJoint annual conferenceof Biomedical sciences.第 26 屆生物醫學聯合學術年會。
14. A Novel L-Amino Acid Oxidase fromTrichoderma harzianumETS 323 Associated with Antagonism ofRhizoctonia solani.楊家安 1*,李政偉 2,劉淑瑛 3,羅朝村 4,
5#
March 19, 2011, Taipei, ROC. The 26thJoint annual conference of Biomedical sciences.第 26 屆生物醫學聯合學術年會。
15. Cloning, Overexpression, and Characterization of a Novel FungalL-Phenylalanine Oxidase fromTrichoderma harzianumETS 323.鄭琪樺 1*,羅朝村 2,劉淑瑛 3,
1#
March 19, 2011, Taipei, ROC. The 26thJoint annual conference of Biomedical sciences.第 26 屆生物醫學聯合學術年會。
16. Construction and characterization of ThSM1 transgenic tobacco. Hong-shin Lin1*, Kou-Cheng Peng1#, Kuo-Chih Lin1#, Chaur Tsuen Lo2, Shu-Ying Liu3March 1
Taipei, ROC. The 26thJoint annual conference of Biomedical sciences.第 26 屆生物醫學聯合學術年會。
17. Chrysophanol Induces Various Pathogen Related proteins and Enhances Plant Resistance Against Fungal Pathogen. Marthandam Asokan Shibu1*, Kou-Cheng
Chaur Tsuen Lo2, Shu-Ying Liu3March 19, 2011, Taipei, ROC. The 26thJoint annual conference of Biomedical sciences.第 26 屆生物醫學聯合學術年會。
18. Isolation ofTrichodermaAntifungal Metabolites. Hui-Nung Shih1*, Chaur Tsuen Lo2, Shu-Ying Liu3, Kou-Cheng Peng1#March 19, 2011, Taipei, ROC. The 26thJoin
conference of Biomedical sciences.第 26 屆生物醫學聯合學術年會。
19. Expression ofTrichoderma harzianumETS 323 cellulase genes inEscherichia coliBL21. Bo-Yuan Huang1*, Kou-Cheng Peng1#, Chaur Tsuen Lo2, Shu-Ying Liu3M
2011, Taipei, ROC. The 26thJoint annual conference of Biomedical sciences.第 26 屆生物醫學聯合學術年會。
20. Evaluation and characterization of antifungal compounds from the fermented products of Trichoderma harzianum SL-BNR1-6. Shu-Ying Liu, Fong-Ji Cai, Mart
Asokan Shibu, Chaur-Tsuen Lo, Kou-Cheng Peng. Hawaii, USA, August 6-10, 2011. The American Phytopathological Society (APS) and the International Ass
for Plant Protection Sciences (IAPPS) joint meeting (2011 APS-IPPC Joint Meeting).
21. Biochemical and antibacterial properties of L-amino acid oxidase derived fromTrichoderma harzianumETS323. Shu-Ying Liu, Chi-Hua Cheng, Chaur-Ts
Kou-Cheng Peng, Chia-Ann Yang. Hawaii, USA, August 6-10, 2011. The American Phytopathological Society (APS) and the International Association fo
Protection Sciences (IAPPS) joint meeting (2011 APS-IPPC Joint Meeting).
22. AntagonismTrichoderma harzianumETS 323 andBotrytis cinereaassociated with L-phenylalanine oxidase-incuced reactive oxygen species generation. Shu-Yi
Chia-Ann Yang, Kou-Cheng Peng, Chaur-Tsuen Lo, Chi-Hua Cheng. Hawaii, USA, August 6-10, 2011. The American Phytopathological Society (APS)
International Association for Plant Protection Sciences (IAPPS) joint meeting (2011 APS-IPPC Joint Meeting).
23. Screening strains ofTrichodermaspp. for decomposition of agriculture wastes. Chaur-Tsuen Lo, Ji-Der Hsieh, Kou-Cheng Peng. Hawaii, USA, August 6-10, 20
American Phytopathological Society (APS) and the International Association for Plant Protection Sciences (IAPPS) joint meeting (2011 APS-IPPC Joint Meeting)
24. 以纖維素酶磁性奈米顆粒分解纖維素生成葡萄糖彭國証，2012 植物保護製劑 Face to Face 技術商談媒合會
25. 木黴菌 anthraquinones之抑病原菌及增強植物免疫力功效彭國証，2012 植物保護製劑 Face to Face 技術商談媒合會
26. Activation of a glutathione S-transferase and a deoxycytidine deaminase expression in Brassica oleracea var capitata that correlates with resistance to Rhizoctoni
challenge during Trichoderma harzianum ETS 323 association. MARTHANDAM ASOKAN SHIBU, Hung-Hsun Lin, Hsueh-Hui Yang, Shu-Ying Liu, Chaur-Ts
Kou-Cheng Peng*. Trichoderma and Gliocladium 2012 Conference.
27. Screening strains and mechanisms of Trichoderma spp for phosphate solubilization. Chaur-Tsuen, Lo*, Pei-Char Feng and K.C. Peng. Trichoderma and Gliocladiu
Conference.
28. Illumination Effects on Trichoderma harzianum ETS 323 Cellulases and Anthraquinones Production. Chih-Wei Hsu*, Chaur-Tsuen Lo, Kou-Cheng Peng ,Shu-Yin
Trichoderma and Gliocladium 2012 Conference.
29. Antimicrobial activity of Trichoderma harzianum L-amino-acid oxidase N-terminal sequence derived peptides. HONG-SHIN LIN, Shu-Ying Liu, Kou-Chen
Chaur-Tsuen Lo* . Trichoderma and Gliocladium 2012 Conference.
四、其他著作或專利：
1. United States Patent 8097441: Ethanol production from graminaceous plants by using immobilized carbohydrate hydrolases on nanoparticles. 2012.
五、研究計畫：
起迄年月
研究計畫名稱
誘導作物產生抑病之
95 年 1 月迄 12 月
木黴菌代謝物質分析與檢測方法之建立（一）
主持人/共同主持人
主持人
誘導作物產生抑病之
主持人
96 年 1 月迄 12 月
木黴菌代謝物質分析與檢測方法之建立（二）
誘導作物產生抑病之
主持人
97 年 1 月迄 12 月
木黴菌代謝物質分析與檢測方法之建立（三）
化學生物與資訊人才培育計畫~
主持人
97 年 1 月迄 12 月
I.蛋白質結構與功能之生物基礎
化學生物與資訊人才培育計畫~
主持人
98 年 1 月迄 12 月
I.蛋白質結構與功能之生物基礎
化學生物與資訊人才培育計畫~
主持人
99 年 1 月迄 12 月
I.探討流感病毒蛋白質結構與特性
98 年 2 月迄 12 月
開發台灣本土性木黴菌纖維素水解酵素
共同主持人
98 年 8 月迄 99 年 7 月
木黴菌抗菌物質之開發與應用
主持人
99 年 8 月迄 100 年 7 月
木黴菌抗菌物質之開發與應用
主持人
99 年 9 月迄 100 年 7 月
99 年 4 月迄 99 年 12 月
木黴菌抗菌物質之開發與應
主持人
(延攬博士後研究人才)
生物農藥及其他非農藥防治資材之開發與應用
主持人
100 年 1 月迄 100 年 12 月 生物農藥及其他非農藥防治資材之開發與應用
主持人
101 年 1 月迄 101 年 12 月 生物農藥及其他非農藥防治資材之開發與應用
主持人
木黴菌生物防治物質之開發與應用－
主持人
101 年 08 月 01 日起
〈子計畫二〉木黴菌三向生物防治之
至
次代謝物物質純化、鑑定、
102 年 07 月 31 日
與作物誘導作物抗性之研究與應用
101 年 08 月 01 日起
至
102 年 07 月 31 日
研究木黴菌 L 胺基酸氧化脢之抗微生物機制
主持人
101 年 12 月 01 日起
至
102 年 10 月 31 日
學制 : 學士班
應用新興科技提高有機質肥效
及其教學發展計劃－子計畫–︰
有機質肥奈米與酵成技術及其教學發展計畫（1/3）
共同主持人