Supplemental Figure 1.
Summary of locations of the archaeological sites of human skeletal remains analysed in this study.
Present territory of Hungary is shown in a more brightly-coloured area. Legends:  Neolithic KSC site,
 Neolithic ALP site,  Late Neolithic site,  Medieval site,18-19th century site (Vác),  Hungarian
Natural History Museum, Budapest. Numbers mark the excavations as follows: 1_Aszód, 2_Csongrád,
3_Dobóruszka,
4_Ecsegfalva,
5_Folyás,
6_Kisköre,
7_Mezőkövesd,
8_Szakmár,
9_Szarvas,
10_Szegvár, 11_Vác, 12_Vörs, 13_Zalavár, 14_Budapest.
Supplemental Figure 2.
A
Summary of the PCR strategy for the amplification of human mtDNA to reconstruct ancient
sequences of interest. Positions of the overlapping regions on the HVS-I depicted by the start
nucleotides of designed primers used in this study.
Starting nucleotide positions of primers are determined by the revised CRS 26 and expected amplicon
sizes are indicated . Amplicon B1 amplified with primer pair LAF-LAR, B2 with LAF-LBR, B3 with
LAF-LCR, B4 with LAF-LDR, B7 with LBF-LCR, B10 with LCF-LCR, B11 with LCF-LDR, B12
with LDF-LCR, B13 with LDF-LDR.
B
Alignment reconstructed HVS-I sequence of the Szarvas 23/20 remain to the revised Cambridge
Reference Sequence (mit rCRS) with specific control mutations depicted.
Supplemental Table 1.
Summary of SNP-PCR results for control polymorphism C16257A and C16261T in the
archaeological mtDNA samples.
For archaeological remains refer to Table 2. in the main article. +; detected DNA fragments amplified
by SNP-PCR , -; no detectable DNA by the polymorphic PCRs, Nd; not detected by the polymorphic
PCRs. Abbreviations: M1C or M1A: forward primers for control polymorphism C16257A with C or
A nucleotide alteration at the 3’ end of each primer, respectively. M2C, M2A, M2G, M2T: forward
primers for control polymorphism C16261T with C, A, G or T nucleotide alterations at the 3’ end of
each primer, respectively.