USDA ARS GEM Field Day 2006 GEM MINI-NURSERY BOOK GERMPLASM ENHANCEMENT OF MAIZE Field Day - Sept. 21, 2006 DEMONSTRATION PLOT MAP N ENTRY NUMBERS RUN FROM WEST TO EAST 1 75 RANGE 1 Breeding History and Inbreds 150 76 RANGE 2 Top Crosses Field History: Fertilizer: Herbicide: Insecticide: Planted on April 27th, 2006 Total: N 120 lbs/acre, P 40 lbs/acre, and K 40 lbs/acre PPI: Harness 2 pt/acre None GEM homepage: www.public.iastate.edu/~usda-gem Page 1 of 12 USDA ARS GEM Field Day 2006 Germplasm Enhancement of Maize Project USDA-ARS North Central Regional Plant introduction Station Ames, Iowa September 21, 2006 The Germplasm Enhancement of Maize (GEM) project is a cooperative effort of the United States Department of Agriculture’s Agricultural Research Service (USDA-ARS), universities, private industry, international, and non-governmental (NGO) organizations to collaboratively broaden the germplasm base and develop enhanced germplasm from exotic sources. The GEM Project is administered through the USDA-ARS Plant Introduction Research Unit in Ames, IA (Research Leader, Candice Gardner), and the Plant Science Research Unit in Raleigh, NC. The sources of germplasm include exotic temperate and tropical accessions identified by the Latin American Maize Project (LAMP), and from North Central Regional Plant Introduction Station (NCRPIS) maize collection. Other exotic sources include material from Thailand, CIMMYT, etc. The exotic accessions are crossed with adapted proprietary inbreds provided by GEM cooperator companies as part of “in kind support” of the project. Presently GEM has cooperators from 22 private companies, 20 public institutes, one NGO, and 10 international cooperators. Guidance to the GEM project is provided by the GEM Technical Steering Group (TSG) with 10 members representing private industry and the public sector. Breeding history and GEM breeding methods (entries 1-29): Maize has an interesting history of domestication and improvement beginning with the wild relative, teosinthe more than 9,000 years ago. Improvements include better yield, stalk lodging resistance, disease/insect resistance, and stress tolerance. Entries 1-29 are side by side observations of germplasm used in the US Corn Belt. The original Stiff Stalk Synthetic (SS) developed by George Sprague in the early 1930’s, the improved cycles of SS, and derived lines from SS follow. A new feature in 2006 includes some expired PVP lines planted adjacent to the public lines that were involved in the pedigree parentage of the PVP lines. Improvements can be seen for stay green, stalk lodging, and general appearance as the era of breeding advances towards present day germplasm. The most popular double cross of the 1930’s include US 13, and this hybrid and the four parental lines are planted in the demo plot along side B73 x Mo17, and GEM top cross hybrids. The GEM breeding protocol is a modified pedigree method originating from crosses to exotic accessions made by private GEM cooperators (with their proprietary lines). Early generation testing starts at the S2 stage (first year trials), and S3 stage (second year trials). The testers used are elite proprietary lines. In the Midwest, the breeding focus is for 25% tropical breeding crosses, and 25% and 50% temperate breeding crosses. The GEM program in Raleigh, NC focuses on 50% tropical breeding crosses. Recommended and re-test lines, and their topcross hybrids (entries 30-93, with corresponding top crosses in south range from 58 to 121. Retest hybrids from 122-131) GEM inbreds are recommended each year to GEM cooperators based on 2 year’s of trial data. Some of the recommended lines (years 2004-2006) also have value-added traits including disease resistance, % protein, protein quality (amino acids), oil, and starch properties. Also included in this section are GEM lines that are now available to the public. GEM homepage: www.public.iastate.edu/~usda-gem Page 2 of 12 USDA ARS GEM Field Day 2006 Value-added traits (VAT) and Methods (distributed throughout the plot and designated on signs) Grain quality analysis is conducted in our labs in Ames managed by Sue Duvick, USDA-ARS. All VAT data was taken on seed derived from self pollinated lines. No VAT data is available for top crosses although we plan to collect top cross data in 2007. Near infrared spectroscopy (NIR) analysis is done on shelled grain to determine protein, oil, and starch content. GEM target values include protein of 13%, oil 6%, and starch 75%. Amino acid analysis is being studied in collaboration with Paul Scott, USDA-ARS, Corn Insects and Plant Genetics Research Unit in Ames. A protein quality index is used based on the three limiting amino acids-methionine, lysine, and tryptophan. The method used for amino acid analysis is the microbial method reported by Scott et al. in 2004 (Maydica 49: 303-311). Starch thermal properties are analyzed by Differential Scanning Calorimetry (DSC) to determine functionality and potential for food and industrial applications. Further starch research is done by Dr. Jay-lin Jane, Iowa State Department of Food Science and Human Nutrition. Dr. Jane’s research includes the molecular analysis of amylopectin branching, enzymology for starch digestibility, and new lab methods to identify germplasm with “resistant” starch. Resistant starch is now receiving increased attention for human nutrition, diabetes, and obesity. Public Cooperator Research and released public GEM lines: Germplasm developed by public cooperators through specific cooperator agreements (SCA) include 7 Universities. Most of these lines are publicly available and have one or more attributes such as VAT’s, disease resistance, mycotoxin resistance, drought tolerance, and silage yield/quality. North Carolina State University (entries 52-58 and corresponding top crosses 93-99): North Carolina State focuses on the development of 50% tropical germplasm under the leadership of Dr. Major Goodman. In addition to the above entries, public GEM releases from this program include entry 58 (GEMS-0016), entry 61 (GEMS-0030), and entry 64 (GEMS-0003). The North Carolina GEM lines have interesting attributes that include leaf resistance, Fusarium ear rot resistance, high protein, oil, and above average amino acid balance. Twenty-nine registered GEM lines in 2006 are publicly available through NCRPIS, and listed in Crop Sci. 46:996-998, and Crop Sci. 46:1825-1826. More information can be found in Public Cooperator Report 2005, Goodman, on GEM web site. Ohio State University (entry 63 and corresponding top cross entry 88): Dr. Richard Pratt developed GEMS-0002 under disease and stress environments in Ohio, with collaborative breeding and trial support from GEM private cooperators, and the USDA-ARS in Ames. GEMS-0002 is a SS line derived from the 50% exotic breeding cross, FS8A(S):S09. GEMS-0002 is 2 days earlier flowering than B73 in Ames, and has fast dry down with good stress tolerance. GEMS-0002 had outstanding fusarium ear rot and fumonisin resistance in 2005, and studies are being repeated in 2006. The line was registered in 2005, and is publicly available through the NCRPIS. See Crop Sci. 45:2130-2131. University of Delaware (entries 66-68 and corresponding top crosses 83-85): Dr. Jim Hawk developed DE3 and DE4 which are non-stiff stalk lines derived from the Brazilian 50% tropical breeding cross, DKXL212:N11a. DE4 is a good source for gray leafspot resistance. The experimental line, DKXL212:N11a-139 has above average amino acid index (three year’s data, Paul Scott Lab, USDA-ARS, Ames, IA). DE3 and DE4 were registered in 2005. See Crop Sci. 45:26692670. GEM homepage: www.public.iastate.edu/~usda-gem Page 3 of 12 USDA ARS GEM Field Day 2006 Texas A&M University (entries 70-71 and corresponding top crosses 80-82): Dr. Wenwei Xu’s research in Lubbock, TX focuses on the development of lines and breeding methods for abiotic stress tolerance. Tx204 and Tx205 are NS sister lines and derived from the 25% breeding cross, AR01150:N0406. Tx204 has a white cob, and Tx205 has a red cob. The lines have excellent silking ability; stay green; and are resistant to leaf firing under heat and drought stress. Both lines have good combining ability with LH200, B110, B113, and are publicly available through Texas A&M. See Public Cooperator Report 2005, Xu, on GEM web site. University of Wisconsin (entries 132-134): W605S was developed by Dr. Jim Coors in 2004. Inbred W605S (a non-stiff stalk derived from the 25% breeding cross, AR17056:N1019) was released for its high milk production index per acre, and high neutral detergent fiber digestibility (NDFD). The research in Wisconsin includes breeding methods to enhance silage yield and nutritional quality with exotic germplasm. W605S is publicly available from the University of Wisconsin. See Public Cooperator Report, 2005, Coors, on GEM web site, and Silage Breeding website, University of Wisconsin. Cornell University (entries 135-137): Dr. Margaret Smith plans to release 3 GEM lines with anthracnose resistance in the near future. Planted in the demo plot are top crosses with lines from FS8B(T), and AR01150 from the US and Argentina respectively. See Public Cooperator Report 2005, Smith, on GEM web site. Truman State University (entries 138-140): Top crosses with Amylomaize VII lines (70% amylose) are from Dr. Mark Campbell’s research using exotic germplasm from the GEM Project. The 50% tropical breeding cross, GUAT209:S13 was found to be a source of modifier alleles to enhance amylose levels when combined with sources having the amylose extender allele, ae. This germplasm (GUAT209:S13, S4 generation) will be publicly released as GEMS-0067 in the near future. Research now in progress includes identifying and mapping the modifier allele(s) with molecular markers. The three top crosses planted in the demonstration include converted GEM lines derived from Mexican Tuxpeno, Brazilian, US, and Cuban germplasm that have been converted to 70% amylose using the GUAT209:S13 source. All of the hybrids are fully GEM x GEM on both sides of pedigree. See Public Cooperator Report 2005, Campbell, on GEM web site. GEM breeding crosses (entries 72-78) and allelic diversity germplasm (entries 141-150): Some of the breeding crosses that have been found to be useful sources of germplasm are shown in the plot with the corresponding lines identified that were derived from these breeding crosses. All of the breeding crosses are 25% exotic and comprise about five races of maize. The allelic diversity germplasm represents new races that have not been used by GEM. Some of the races include tropical Highland that are very difficult to work in US environments. More information on the structure and organization of the GEM Project, membership, yield trial results, germplasm releases, and summaries of University and USDA-ARS research can be found on our web site, http://www.public.iastate.edu/~usda-gem/. Information and public availability of the LAMP accessions used by GEM can be found at the Germplasm Resources Information Network (GRIN) at the web site, http://www.ars-grin.gov. GEM homepage: www.public.iastate.edu/~usda-gem Page 4 of 12 USDA ARS GEM Field Day 2006 2006 GEM Field Day (Planted on April 27th) Entry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Entry 30 121 Pedigree Corn x teosinthe (wild relative) Teosinthe (wild relative of corn) Original Stiff Stalk Synthetic (SSS C0) B14 LH74 Stiff Stalk Synthetic cycle 5 B73 LH132 BS13(S2)C1 B84 Lancaster Sure crop Mo17 LH51 Oh43 LH39 Wf9 38-11 L317 Hy Wf9 X 38-11 L317 X Hy U.S. 13 double cross hybrid Cuba 164 CUBA164:S20 CUBA164:S2012 CUBA164:S2012-459-001-B CUBA164:S2012-459-001-B x LH185 B73xMo17 LH198 x LH185 Pedigree AR17056:N2035-421-001-B-B AR17056:N2035-421-001xLH198 GEM homepage: www.public.iastate.edu/~usda-gem Shed Date Silk Date 80 84 80 80 84 82 81 83 71 82 74 78 80 80 87 84 83 77 80 77 106 84 83 84 78 79 78 82 91 81 82 90 83 83 86 74 89 77 79 79 83 92 92 92 80 84 83 109 92 85 89 79 81 78 Shed Date Silk Date 83 78 86 79 History Zea mays subspecies parviglumis Zea mays subspecies parviglumis Derived from 16 line synthetic Public inbred derived from SSS CO Related to inbreds B14 and B73 Synthetic population that B73 was derived from Public inbred derived from SSS cycle 5 Related to B73 and B37 B84 was derived from BS13(S2)C0 Public inbred derived from BS13(S2)C0 O.P. that C103 was derived from Public inbred derived from C103 x 187-2 Related to Mo17 Public inbred derived from Oh40B x W8 Related to Oh43 Parent line of US 13 double cross Parent line of US 13 double cross Parent line of US 13 double cross Parent line of US 13 double cross Single cross parent of US 13 double cross Single cross parent of US 13 double cross Most popular hybrid of 1930's-1940's Tropical GEM accession (with many traits) 50% Tropical Breeding Cross 25% Tropical Breeding Cross S3 selection from entry 25 Top Cross of entry 26 to LH185 Popular hybrid from mid-1970's - 1980's Medium maturity check hybrid Comments Developed in 1930's by Sprague Released in 1953 Expired PVP Released in 1972 Expired PVP Released in 1979 Released in 1964 Expired PVP Released in 1949 Expired PVP Origin: Indiana 1936, Wilson Farm Reid Yell. Dent Origin: Indiana 1930's, Reid Yell. Dent Origin: Iowa, 1930's, Richey Lancaster Origin: Illinois, 1930's, Illinois High Yield Lines and Top Crosses 2006 Recommended line Top Cross Page 5 of 12 USDA ARS GEM Field Day 2006 31 120 AR17056:N2035-473-001-B-B AR17056:N2035-473-001xLH198 83 80 85 79 2006 Recommended line Top Cross 32 119 BR52060:S0210-143-001-B-B BR52060:S0210-143-001xLH185 85 77 89 79 2006 Recommended line Top Cross 33 118 BR52060:S0210-147-001-B-B BR52060:S0210-147-001xLH185 87 78 89 79 2006 Recommended line, 5.0% oil Top Cross 34 117 MDI022:N2120-284-001-B-B MDI022:N2120-284-001/LH200 86 82 87 84 2006 Recommended line Top Cross 35 116 MDI022:N2120-333-001-B-B MDI022:N2120-333-001xLH198 84 83 89 84 2006 Recommended line, Above average methionine content Top Cross 36 115 NEI9004:S2818-003-001-B-B NEI9004:S2818-003-001xLH185 84 78 87 80 2006 Recommended line, 13.5% protein Top Cross 37 114 NEI9004:S2818-025-001-B-B NEI9004:S2818-025-001xLH185 84 79 87 79 2006 Recommended line, 13.7% protein Top Cross 38 113 NEI9004:S2818-376-001-B-B NEI9004:S2818-376-001xLH185 83 78 85 80 2006 Recommended line, 13.8% protein Top Cross 39 112 UR05017:S0415-187-001-B UR05017:S0415-187-001xLH185 87 76 89 79 2006 Recommended line, 13.1% protein Top Cross 40 111 UR05017:S0415-180-002-B-B UR05017:S0415-180-002xLH185 82 76 85 79 2006 Recommended line, 15.6% protein, Fusarium resistant Top Cross 41 110 BR51675:N0620-033-001-B-B BR51675:N0620-033-001xLH198 81 80 82 79 2006 Recommended line, good Y/M ratio Top Cross 42 109 BR51675:N0620-053-001-B-B BR51675:N0620-053-001xLH198 82 77 82 77 2006 Recommended line Top Cross 43 108 CH05015:N1204-057-001-B-B-B CH05015:N1204-057-1-B/LH198 82 77 85 78 2005 Recommended line, 14.0% protein, 4.7% oil Top Cross GEM homepage: www.public.iastate.edu/~usda-gem Page 6 of 12 USDA ARS GEM Field Day 2006 44 107 DKB844:S1601-003-002-B-B-B-B-B DKB844:S1601-003-2-B-B/LH185 85 78 88 81 2005 Recommended line, 14.8% protein, and low starch gelatinization temp Top Cross 45 106 DKB844:S1601-073-001-B-B-B-B-B DKB844:S1601-073-1-B-B/LH185 85 78 87 79 2005 Recommended line, low starch gelatinization temperature Top Cross 46 105 CHIS775:S1911b-120-001-B-B-B-B CHIS775:S1911b-120-1-B-B/LH185 83 77 85 77 2005 Recommended line, starch thermal properties research Top Cross 47 104 AR17056:N2025-574-001-B-B-B-B AR17056:N2025-574-1-B-B/LH198 82 76 84 77 Highly digestible starch, 65%, 16.3% protein Top Cross 48 103 UR11003:S0302-1011-001-B-B-B UR11003:S0302-1011-1-B/LH185 81 76 83 79 2005 Recommended line Top Cross 49 102 FS8B(T):N11a-87-1-B-B-sib FS8B(T):N11a-087-1-B/LH200 80 78 82 86 2004 Recommended line, good Y/M ratio Top Cross 50 101 AR03056:N09-182-001-B-B-B-Sib-B AR03056:N09-182-1-B-B x LH198 82 77 85 80 2004 Recommended line, good Y/M ratio Top Cross 51 100 B73 LH198 x LH185 84 76 87 76 Medium maturity check inbred Medium maturity check hybrid 52 99 7541-7-32-1-B-B-B-B_DKXL380 N11 F2S6 DKXL380 N11 F2S6 x (LH132 x LH195) 93 80 96 81 2006 Recommended line for South (NC State U) Top Cross 53 98 1881-002/98_DKXL370AN11F2S3_7521-05-B 1881-002/98 x (FR992 x FR1064) 92 77 95 77 2005 Recommended line for South, 14.6% protein (NC State U) Top Cross 54 97 1883-002/98_DKXL370AN11F2S3_7521-05-B 1883-002/98 x (FR992 x FR1064) 91 80 95 81 2005 Recommended line for South (NC State U) Top Cross 55 96 1886-003/98_DKXL370AN11F2S3_7521-05 1886-003/98 x (LH132 x LH195) 92 81 95 83 2005 Recommended line for South, 13.9% protein (NC State U) Top Cross GEM homepage: www.public.iastate.edu/~usda-gem Page 7 of 12 USDA ARS GEM Field Day 2006 56 95 1895-001/98_DKXL370AN11F2S3_7521-29-B 1895-001/98 x HC33 87 77 91 80 2005 Recommended line for South, 13.8% protein (NC State U) Top Cross 57 94 2410-003/99_SCRGp3N14F2S3_7258-3-B 2410-003/99 x HC33 93 80 91 80 2005 Recommended line for South (NC State U) Top Cross 58 93 GEMS-0016 GEMS-0016 x LH185 85 77 87 79 Gray leafspot resistant (NC State U/NCRPIS) Top Cross 59 92 AR16035:S02-450-001-B-B-B-B-B-B AR16035:S02-450-1-B-B/LH185 90 78 92 79 Orange endosperm for grain carotene research Top Cross 60 91 DKXL212:N11a-139-001-001-B-B-B-B-B DKXL212:N11a-139-001-001-B-B-B-B x LH119 86 78 88 81 Above average amino acid index (UDEL) Top Cross 61 90 GEMS-0030 GEMS-0030 X LH283 95 81 97 83 Above average amino acid index (NC State U/NCRPIS) Top Cross 62 89 GEMS-0030 GEMS-0030 x LH123 92 81 95 83 Above average amino acid index (NC State U/NCRPIS) Top Cross 63 88 GEMS-0002 GEMS-0002 x LH185 80 77 83 77 Fusarium grain mold resistant (OH State U/NCRPIS) Top Cross 64 87 GEMS-0003 GEMS-0003 x LH283 87 82 90 83 Fusarium and fumonisin resistant (NC State U/NCRPIS) Top Cross 65 86 GEMS-0061 GEMS-0061 x LH185 84 77 87 81 Fusarium grain mold resistant, 4.7% oil, Public Release NCRPIS Top Cross 66 85 DE3 LH381 X DE3 81 81 82 85 Deriv DKXL212:N11a-191-1-1-1-1-1-1 (UDEL) Top Cross 67 84 DE4 LH381 X DE4 83 81 85 83 Gray leafspot resistant (UDEL) Top Cross GEM homepage: www.public.iastate.edu/~usda-gem Page 8 of 12 USDA ARS GEM Field Day 2006 68 83 DKXL212:N11a-139-001-001-B-B-B-B-B LH381 x DKXL212:N11a-139-001-001-B-B-B 84 82 87 86 Above average amino acid index (UDEL) Top Cross 69 82 B73 LH200 x LH262 83 83 87 86 Medium maturity check inbred Late maturity check hybrid 70 81 Tx204 LH200 x TX204 85 81 83 83 Drought and heat tolerant (Texas A&M) Top Cross Silking before anthesis 71 79 80 Tx205 B113 x Tx205 TX205 x LH200 85 78 81 84 80 83 Drought and heat tolerant (Texas A&M) Top Cross Top Cross Silking before anthesis Shed Date Silk Date 81 80 77 79 79 81 81 83 81 83 80 80 79 85 Shed Date Silk Date 80 77 77 77 77 78 76 78 80 81 76 76 77 77 81 79 78 77 77 79 79 80 81 84 77 79 79 79 Entry 72 73 74 75 76 77 78 Entry 122 123 124 125 126 127 128 129 130 131 132 133 134 135 Pedigree NEI9004:S2818 MDI022:N2120 AR17056:N2035 DKB844:S1601 CH05015:N1204 UR13085:N0215 CHIS775:S1911b Pedigree CASH:N1410-025-001/HC33 CASH:N1410-018-001/HC33 HC33 x LH287 UR11003:S17a12-300-001/LH287 UR11003:S17a12-054-001/LH287 NEI9004:S2818-788-001/LH287 NEI9004:S2818-761-001/LH287 CUBA164:S2012-596-001/LH287 CUBA117:S1542-037-002/LH287 CUBA117:S1542-015-001/LH287 W605S x LH198 W605S x LH244 W605S x LH332 LH198 x AR01150:N0406-266 GEM homepage: www.public.iastate.edu/~usda-gem Breeding Crosses Origin: Origin: Origin: Origin: Origin: Origin: Origin: Thailand Race: Suwan Peru Race: Cuban Yellow Dent Argentina Race: Cristalino Colorado Mexico Race: Tropical Hybrid Chile Race: Camelia Uruguay Race: Cateto Sulino Mexico Race: Tuxpeño SS breeding cross nSS breeding cross nSS breeding cross SS breeding cross nSS breeding cross nSS breeding cross SS breeding cross Re-Test Top Crosses 2006 Retests 2006 Retests Medium maturity check hybrid 2006 Retests 2006 Retests 2006 Retests 2006 Retests 2006 Retests 2006 Retests 2006 Retests Silage Top Cross (U WIS) Silage Top Cross (U WIS) Silage Top Cross (U WIS) Anthracnose Resistant Top Cross (Cornell) Page 9 of 12 USDA ARS GEM Field Day 2006 136 137 LH198 x FS8B(T):N1802-212 LH198 x FS8B(T):N1802-215 77 77 78 79 Anthracnose Resistant Top Cross (Cornell) Anthracnose Resistant Top Cross (Cornell) 138 CHIS740:S1411a-783-2-B-B ae x FS8B(T):N11a-322-1-B-B ae 79 83 Amylomaize VII Hybrid (Truman State U) 139 CUBA164:S1511b-325-1-B-B ae x DKXL370:N11a20-234-2B-B-B ae 81 81 Amylomaize VII Hybrid (Truman State U) 140 CUBA164:S2012-966-1-B-B ae x DKXL370:N11a20-199-2-BB-B ae 81 83 Amylomaize VII Hybrid (Truman State U) Entry Pedigree (PVP x Race) Shed Date Silk Date Allelic Diversity Program 141 LH132:B-/CHANDELLE [CUB68]{CIMYT}-001 84 85 Origin: Lowland Cuba 142 143 144 145 146 147 148 149 150 LH132:B-/N DE T CALIENTE [GUA159]{CIMYT}-001 MBNA:B-1/GUIRUA [MAG469]{ICA}-001 LH39:B-/CANGUIL GRUESO [ECU447]{ICA}-001 LH74:B-/SAN MARCENO # [GUA724]{INIA}-001 LH74:B-/OLOTON [GUA383]{CIMYT}-001 LH74:B-/GORDO [CHH131]{CIMYT}-001 G39:B-/CONICO [PUE116]{CIMYT}-001 G39/(G39/CONICO [PUE116]{CIMYT}) B47:B-/CONICO [PUE116]{CIMYT}-001 84 93 74 93 93 73 81 82 79 92 104 77 97 96 76 84 84 83 Origin: Lowland Guatemala Origin: Mid-Highland Columbia Origin: Highland Guatemala Origin: Highland Guatemala Origin: Highland Guatemala Origin: Highland Mexico Origin: Highland Mexico Origin: Highland Mexico Origin: Highland Mexico GEM homepage: www.public.iastate.edu/~usda-gem Page 10 of 12 USDA ARS GEM Field Day 2006 GEM Accessions 2006 Field Day All Publicly Available from NCRPIS Accession PI Number Country Classification Race or Source Color/type ARZM 01150 PI 491741 Argentina Temperate Dent. Blanco Rugoso White dent ARZM 03056 PI 491799 Argentina Temperate Dentado Blanco White dent ARZM 16026 PI 516027 Argentina Temperate Cristalino Colorado Orange flint ARZM 16035 PI 516036 Argentina Temperate Cristalino Colorado Orange flint ARZM 17026 PI 493012 Argentina Temperate Cristalino Colorado Orange flint ARZM 17056 PI 493039 Argentina Temperate Cristalino Colorado Orange flint BRA 051675 (PE 027) PI 584913 Brazil Tropical Dente Amarelo Yellow dent BRA 052051 (SE 032) PI 583917 Brazil Tropical Dente Amarelo Yellow dent BRA 052060 (SE 033) PI 583918 Brazil Tropical Dente Amarelo Yellow dent Cash (Ohio) PI 278710 USA Temperate Corn Belt Dent Yellow dent CHIS 740 PI 583890 Mexico Tropical Tuxpeño/Olotillo White dent CHIS 775 PI 576258 Mexico Tropical Tuxpeño White dent CHZM 05015 PI 467165 Chile Temperate Camelia Orange flint Cuba 117 PI 483816 Cuba Tropical Argentino Orange flint Cuba 164 PI 489361 Cuba Tropical Mixed (Creole) Orange semi-flint DK888 Ames 23669 Thailand Tropical Hybrid Yellow DKB844 Ames 23675 Mexico Tropical Hybrid White DKXL212 Ames 23673 Brazil Tropical Hybrid Yellow DKXL370A Ames 23671 Brazil Tropical Hybrid Yellow DKXL380 Ames 23672 Brazil Tropical Hybrid Yellow FS8A(S) (Florida) PI 536619 USA Temperate Mixed Yellow semi-dent FS8B(T) (Florida) PI 536622 USA Temperate Mixed Yellow semi-dent Guatemala 209 PI 498583 Guatemala Tropical Tusón Yellow flint MDI022 PI 571994 Peru Tropical Cuban yellow Yellow flint NEI9004 Ames 26253 Thailand Tropical Suwan Yellow flint St. Croix Gp3 PI 504148 St. Croix Tropical Tusón Yellow dent URZM 05017 PI 583937 Uruguay Temperate Semidentado Riograndense Orange semi-dent URZM 11003 PI 583939 Uruguay Temperate Dente Branco White dent URZM 13085 PI 583927 Uruguay Temperate Cateto Sulino Orange flint GEM homepage: www.public.iastate.edu/~usda-gem Page 11 of 12 USDA ARS GEM Field Day 2006 Contact Information for Germplasm I. Contact Information for GEM Germplasm Contributors for 2006 Field Day: Mike Blanco (mblanco@iastate.edu) GEM Project Coordinator Mark Campbell (campbell@truman.edu) Truman State University (Kirkville, MO) James G. Coors (jgcoors@facstaff.wisc.edu) University of Wisconsin Major Goodman (goodman@unity.ncsu.edu) North Carolina State University Jim Hawk (jhawk@UDel.Edu) University of Delaware Margaret Smith (mes25@cornell.edu) Cornell University Wenwei Xu (we-xu@tamu.edu) Texas A&M University II. Web Sites for GEM germplasm & other information: Germplasm Enhancement of Maize (GEM) at http://www.public.iastate.edu/~usda-gem Germplasm Resources Information Network (GRIN) at http://www.ars-grin.gov Inbred Lines: GEMS-0002, GEMS-0003, GEMS-0016, GEMS-0030, GEMS-0061, and other public GEM releases University of Wisconsin Silage Breeding at http://www.silagebreeding.agronomy.wisc.edu W605S Inbred Line University of Delaware Maize order forms at http://ag.udel.edu/plsc/research/MaizeResearch.htm DE3 and DE4 Inbred Lines Texas A&M University from Wenwei Xu (we-xu@tamu.edu) Tx204 and Tx205 Inbred Lines III. GEM Staff: Mike Blanco, Mack Shen, Andy Smelser, Fred Engstrom, and Sue Duvick IV. Acknowledgements: Larry Lockhart, Program and Farm Manager Mark Millard, Maize Curator GEM homepage: www.public.iastate.edu/~usda-gem Page 12 of 12