The RNAs and Proteins of dsRNA Viruses: Edited by Peter. P. C. Mertens and Dennis H. Bamford
The dsRNA segments and proteins of simian rotavirus A / SA11 (genus Rotavirus: family Reoviridae) :
Structural studies (17, 31, 37, 72, 73, 88, 89): last updated April 2003, references in [square brackets]
Genome
Segment
†
Size [bp]
1
(3302)
2
(2690)
3
(2591)
ORFs
183282
172659
502554
10 2337
4
(2362)
Gene
Product(s)
(':
Protein
Function)
VP1 (Pol)
VP2 (T1)
VP3 (Cap)
VP4
Protein
Size
aa (Da)
1088
(125005)
881
(102431)
Location in
Virus Particle
Inner capsid,
5-fold axis
Inner capsid
835
(98120)
Inner capsid,
5-fold axis
776
(86782)
Outer capsid
spike
-
VP5*
529
247-776
(60000)
-
VP8*
247
1-247
(28000)
Copy
Number/
Particle
12
120
12
Cognate Proteins‡
Orthoreovirus 3(Pol)
Orbivirus VP 1(Pol)
Coltivirus VP1(Pol)
Cypovirus Pol
Orbivirus: VP3
Orthoreovirus: 1
Orbivirus: VP4
Orthoreovirus: 2
GenBank
Accession
Number (s)
X16830
[56]
RNA-dependent RNA polymerase [87].
Part of minimal replication complex [63,87],
Virus specific 3’-mRNA binding [61,62]
Part of virion transcription complex with VP3 [11,73]
X16831
[56]
Inner capsid structural protein [8].
Non-specific ss & dsRNA-binding activity [10]
Myristoylated [15].
Cleaved [23,86].
Part of minimal replication complex [63].
Leucine zipper [56].
Interacts with VP5 [7].
X16062
[44]
X16387
[56]
120
D16346
[77]
X14204
[55]
1
Functions and Properties
Guanylyltransferase [45,68].
Methyltransferase [13].
Basic Protein [44,56].
Part of virion transcription complex with VP1 [11,73].
Non-specific ssRNA binding [62].
VP4 Dimers form outer capsid spike [3].
Interacts with VP6 [89].
Virus infectivity enhanced by trypsin cleavage of VP4 into
VP5* and VP8* [22,46].
Hemagglutinin [26,38].
Cell attachment protein [47,75,85].
P-type neutralization antigen [32,58].
VP5* permeabilizes membranes [16].
Crystal structure of VP8 fragment (galectin fold) [19].
TRAF2 signaling [43].
Protection [33].
The RNAs and Proteins of dsRNA Viruses: Edited by Peter. P. C. Mertens and Dennis H. Bamford
5
(1611)
311515
NSP1
495
(58654)
L18944
[35]
Nonstructural
0
X14914
[57]
L15384
[48]
6
(1356)
241214
VP6 (T13)
397
(4816)
Middle capsid
780
Orbivirus: VP7
L33365
[48]
M27824
[76]
7
(1105)
26-970
NSP3
315
(34600)
Nonstructural
0
-
M87502
[51]
8
(1059)
47-997
NSP2
(VIP)
317
(36700)
Nonstructural
0
Orbivirus: NS2
Orthoreovirus: NS
L04531
[64]
9
(1062)
491026
VP7
326
[7368)
Outer capsid
glycoprotein
780
-
K02028
[4]
10
(751)
41-569
NSP4
175
(20290)
Nonstructural
0
-
AF087678
[9]
2
Associates with cytoskeleton [34].
Extensive sequence diversity between strains [20,42,57].
Two conserved cysteine-rich zinc-finger motifs [57,60].
Virus specific 5’-mRNA binding [34,62].
Interacts with host IFN regulatory factor 3 [29].
Major virion protein [49,72].
Middle capsid structural protein [72].
Homotrimeric 4o structure [72].
Subgroup antigen [30,39].
Myristoylated [15].
Protection (? Mechanism) [11,84].
Crystal structure [50].
Hydrophobic [48,76].
Homodimer [51,66].
Virus-specific 3’- mRNA binding [69,70].
Binds eIF4G1 and circularizes mRNA on initiation complex
[67].
Involved in translational regulation and host shut-off
[14,59,82].
Crystal structure: NSP3 NH3 fragment with 3’- viral RNA
[17] and NSP3 COOH fragment with eIF4G fragment [31].
Non-specific ssRNA-binding [41,62]
Accumulates in viroplasm [65]
Involved in viroplasm formation with NSP5 [25]
NTPase activity [79]
Helix destabilization activity [78]
Functional octamer [79,80]
Binds NSP5 and VP1 [1, 40]
Regulates NSP5 autophosphorylation [1]
Crystal structure (HIT-like fold) [37]
Outer capsid structural glycoprotein [21,49].
G-type neutralization antigen [32].
N-linked high mannose glycosylation and trimming [21].
RER transmembrane protein, cleaved signal sequence [22].
Ca2+ binding [18].
Protection [33].
Enterotoxin [6].
Receptor for budding of double-layer particle through ER
membrane [5, 53].
RER transmembrane glycoprotein [22].
Ca++/ Sr++ binding site [36].
N-linked high mannose glycosylation [21].
Protection [24].
Host cell [Ca2+]i mobilization [81].
The RNAs and Proteins of dsRNA Viruses: Edited by Peter. P. C. Mertens and Dennis H. Bamford
22-615
NSP5
11
(667)
198
(21725)
Nonstructural
0
X07831
-
[54]
M28347
[83]
80-355
NSP6
92
(11012)
Nonstructural
0
-
Interacts with VP2, NSP2 and NSP6 [1, 27].
Homomultimerizes [27,71].
O-linked glycosylation [28].
(Hyper-) Phosphorylated [2, 83].
Autocatalytic kinase activity enhanced by NSP2 interaction
[2].
Non-specific ssRNA binding [52,62].
Product of second, out-of-frame ORF [52].
Interacts with NSP5 [27].
Localizes to viroplasm [52].
': Protein structure/function: RNA polymerase = A(Pol)@; capping enzyme = A(CaP)@; Inner virus structural protein with T = 13 symmetry =
A(T13)"; viral inclusion body or viroplasm matrix protein = A(ViP)@. Other species within the genus may have proteins with significant
differences in sizes.
†
Segments numbered based on migration of SA11 genome segments in SDS-PAGE gel. Migration order may differ among other members of the
genus.
‡
Proteins with similar functions from other genera.
Updated April 2003, by R.F. Ramig & M.K. Estes
(from : The RNAs and Proteins of dsRNA Viruses: Edited by Peter. P. C. Mertens and
Dennis H. Bamford http://www.iah.bbsrc.ac.uk/dsRNA_virus_proteins/Rotavirus.htm)
Please make suggestions for changes or updates to this table by e-mail to Peter Mertens peter.mertens@bbsrc.ac.uk
3
Table supplied by Eric Mossel , Mary Estes and Frank Ramig
(from : The RNAs and Proteins of dsRNA Viruses: Edited by Peter. P. C. Mertens and Dennis H. Bamford)
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7
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8
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9
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10
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11
Table supplied by Eric Mossel , Mary Estes and Frank Ramig
(from : The RNAs and Proteins of dsRNA Viruses: Edited by Peter. P. C. Mertens and Dennis H. Bamford)
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