Revised 12 February 2016 FIELD Limnology PBIO/ZOOL 719/819 Fall 2005 Instructors: Prof. Alan Baker (Plant Biology) Prof. James Graduate Teaching Assistant: Undergrad Teaching Assistant: Texts & Materials: 1. 2. 3. 4. 5. 6. 7. 8. Office: Spaulding 126 (2-3845) albaker@unh.edu Haney (Zoology) Office: Spaulding 116 (2-2105) jfhaney@unh.edu Sonya Carlson (Zoology) Office: Spaulding 116 (2-2105) scarlson@unh.edu Janet Kleiner jkleiner@unh.edu Common Methods in Limnology, by Lind (recommended) Limnoecology (Lampert & Sommer) (required) Textbook of Limnology (Cole, 4th ed.) (required) A Short Guide to Writing about Biology, by Pechenek (required) USB data storage for PC (required) Data notebook (3 ring) with plastic sheet protectors (required) Clipboard/cover for recording field data (required) Sunny smile and positive attitude (required) Aug 30 Introduction to course: Course Objectives; Research Theme: Diel Vertical Migration (DVM) Learning laboratory techniques: Introduction to laboratory examination and counting of zooplankton. Sep 1 Field Trip 1: Barbadoes Pond (Dover, NH). FOR NEXT CLASS: Stich and Lampert (1981) Sep 6 Lab: Intro to zooplankton and data logger downloading. Computer graphing with SigmaPlot Seminar: 1) PPt presentation & interpretation of lake profiles: Barbadoes Pond trip 2) Discuss Stich & Lampert (1981) Sep 8 Field Trip 2: Barbadoes Pond (Dover, NH). DVM collection Arrive at 3 pm/Return at 10 pm Sep 13 Introduction: Personal gear check for SLCFT (Sawyer Ponds Camping Field Trip) Lab: Zooplankton Identification/Counting Lecture: Light & Temperature Seminar: Discussion of vertical profile plots of DVM at Barbadoes FOR NEXT CLASS: Read: Hansson L.A. (2004) Plasticity in pigmentation induced by conflicting threats from predation and UV radiation. ECOLOGY 85 (4): 1005-1016. Prepare answers: Book Questions #1: L&S Chapter 3: Questions 1, 6 & 7 Sep 15 Seminar: Discuss Book Questions #1& Hansson 2004 Lecture: Solutes Lab: Upload & analyze data: SigmaPlot, zooplankton & phytoplankton counting FOR NEXT CLASS: Read: TBA Prepare answers: Book Questions #2: L&S Chapter 3: Question 4; Chapter 4: Questions 1 & 3 1 Sep 20 Lab: Prepare for SLCFT by labeling containers Seminar: Discuss Book Questions #2 Sep 22 Field Trip 3: Sawyer Ponds (Upper Sawyer and Lower Sawyer Ponds) – Leave Spaulding loading dock at 8 am. Return to Durham campus Friday, September 23 at 2 pm Prepare answers: Book Questions #3: L&S Chapter 4: Questions 7 & 8 Sep 27 Due: Book Questions #3: L&S Chapter 4: Questions 7 & 8 Lab: Upload & analyze data: SigmaPlot, zooplankton & phytoplankton counting Sep 29 Field Trip 3: Mendums Pond Oct 4 Discussion: Introduction to scientific writing and manuscript review (Handouts) Lab: Data uploading and analysis from Mendums Pond Seminar: Discussion of profiles from Mendums Ponds FOR NEXT CLASS: Prepare answers: Book Questions 4: L&S L&S Chapter 4: Questions 11 & 12 Oct 6 Field Trip 4: Russell Pond Leave Spaulding loading dock at 8 am. Return to Durham campus late night Oct 11 Data anaylsis: (1) Fluorometry Data Analysis (2) Epilimnion Chlorophyll Analysis FOR NEXT CLASS: Prepare answers: Book Questions 5: L&S Chapter 6: 7, 8 & 9; L&S Chapter 7: 1 & 2 Oct 13 Discussion: 1) Introduction to scientific writing and manuscript review (handouts), with emphasis on Methods (make list of all methods used) 2) Introduction to statistics: data analysis using parametric and non-parametric tests, means, modes, variance and tests of significance 3) Select lakes and “emphasis” for manuscripts 4) Peek at Russell Pond sonar Lab: Plankton counting & data uploading and analysis from Mendums Pond Seminar: Discuss Book Questions 5 Assignment: Prepare first draft of Methods section due October 18. Oct 18 Field Trip: Stonehouse Pond DVM Meet at 2 pm, Return at 9:30 pm Methods MS due Assignment: Review two METHODS manuscripts (due October 20) Oct 20 Lab: Plankton counting & data uploading and analysis from Russell Pond Data analysis techniques: Regressions Introduction to the use of cladistics Lecture: Diel Vertical Migration (Sonya Carlson) MS Writing: Peer reviews of METHODS MS due 2 Assignments: 1) Write INTRODUCTION (due October 27) 2) Read Papers for Oct 27 Oct 25 Lecture: Origins and Diversity of Lakes and Watersheds Lab: Plankton counting & data uploading and analysis from Stonehouse Pond Data analysis: Examination of fluorescence data Seminar: TBA Oct 27 Lecture: Eutrophication: Causes, consequences and trophic Classification of Lakes Lab: Chaoborus crop analysis and calculation of feeding electivity indicies MS Writing: MS INTRODUCTION: first draft due for peer review Seminar: Impact of Chaoborus predation Nov 1 Lecture: Phytoplankton: Diversity and Competition Lab: Sample processing: Computer Image analysis of plankton body lengths Graphing of plankton profiles MS Writing: Peer Reviews of INTRODUCTION due Assignment: Prepare preliminary manuscript tables and figures (due Nov 8) Nov 3 Lecture: Zooplankton Grazing: Mechanisms and Impact on Phytoplankton Manuscript preparation: Data analysis, graphics and sample processing: Seminar: TBA Nov 8 Lecture: Vertebrate and Invertebrate Predation in the Pelagic Zone Manuscript preparation: Data analysis, graphics and sample processing: Nov 10 Lecture: Biomanipulation as a Lake Restoration Tool Lab: Group projects on Lake Model Videos (fish selective feeding & thermal stratification) Assignment: Review test manuscript (due Nov 17) Manuscript tables and figures due (hardcopies, or PowerPoint files) Nov 15 Lecture: Seasonal and Long-term Succession in Lakes Student DVM Presentations*: Barbadoes Pond & Mendums Pond * 15-min PowerPoint presentations of results (graphs, tables, photos, etc) from phytoplankton and zooplankton emphasis, i.e. two presentations per lake Nov 17 Lecture: Fish and Pelagic Food Webs Manuscript preparation Student DVM Presentations: Russell Pond & Stonehouse Pond Seminar: Comparision of class reviews of the test MS Nov 22 Manuscript preparation Seminar: TBA Nov 24 Thanksgiving Holiday Nov 30 Manuscript preparation Seminar: TBA 3 Dec 1 *Submit DVM manuscripts to editors at 2:00 PM Pick CLS manuscripts to review at 3:00 PM Dec 6 1:00 PM: Reviewers return manuscripts to editors 2:10 PM Authors pick up reviewed manuscripts. 2:20 PM List what manuscript needs: Major changes, minor changes, typographical corrections 2:35 PM List graphs, information, or references needed to revise your manuscript 3:00 PM Conference with instructors to review your progress on your manuscript 4:30 PM Authors revise manuscripts and change to co-authored (CFB journal format) *By the end of class, you are expected to have the abstract, introduction, methods, and 1 table & figure embedded into a new document.* Dec 8 Dec 15 Continue to revise manuscript into co-authored DVM manuscript (CFB journal format) Complete student faculty evaluations Submit Revised DVM manuscript (CFB journal format) to CFB on-line journal (Center for Freshwater Biology Research) 4 Summary of Grading: Manuscript draft Introduction 5% Methods 5% Single Author Manuscript* 15% Co-Authored Manuscript 15% MS reviews Introduction 5% Methods 5% Single Author Manuscript 10% Student Presentations on Lake 5% Student Lectures 10% Book Questions @ 2 pts per hand-in 10% Seminars 10% Class & field trip participation 5% TOTAL 100% *=Single Author Manuscript has emphasis on either phytoplankton or zooplankton Writing Timeline:-Oct 18 Oct 20 Oct 27 Nov 1 Nov 22 Dec 2 Dec 15 Draft 1 of Methods due Review of Methods due Draft 1 of Introduction due Review of Introduction due Single Author DVM Manuscript due Review of Single Author Manuscript due Co-Authored Manuscript due Text topics for student lectures (30 min): 1. Sep 22: Regulation of Phytoplankton (Janet) 2. Oct 6: Lakes and Watersheds 3. Oct 11: Trophic Classification of Lakes 4. Oct 13: Zooplankton Grazing 5. Oct 18: Predation in the Pelagic Zone 6. Oct 20: DVM 7. Oct 25: Seasonal and Long-term Succession in Lakes 8. Oct 27: Pelagic Food Webs and Microbial Loops 9. Nov 1: Fluorescence & Chlorophyll in lakes 5